吻合器痔上黏膜环切术与传统外剥内扎术的对比研究

目的 探讨吻合器痔上黏膜环切术(procedure for proplapse and hemorrhoids,PPH)的适应证、方法、临床应用价值.方法 总结41例PPH手术(Ⅰ组)和62例传统外剥内扎术(Milligan-Morgan手术)(Ⅱ组)临床资料,对手术时间、术后止痛次数、住院时间、术后并发症及住院费用等进行对比研究.结果 总的症状改善满意率分别为95%(Ⅰ组)及82%(Ⅱ组),除住院费用外,Ⅰ组其手术时间、术后止痛次数、住院时间明显优于Ⅱ组,并发症发生率除术后便血、早期大便失禁、早期排尿困难外,余无统计学意义.结论 PPH治疗Ⅲ°～Ⅳ°痔的近期疗效优于Milligan-Morgan手术,且具有安全、有效、手术时间短、住院时间少、恢复快等优点.     

膀胱抗菌机制—粘膜抗菌蛋白初步研究

本文报道以１％乙酸冲洗雌性Ｗｉｓｔａｒ大鼠膀胱和雌性新西兰白兔膀胱，分别获得其膀胱酸溶性提取物。ＡＵ－ＰＡＧＥ分析表明，两种膀胱粘膜酸溶性提取物都有十余条主蛋白带，而不含常见的杀菌物质溶菌酶和防御素样分子。琼脂糖弥散法杀菌试验显示，两种膀胱粘膜酸溶性提取物对致病性大肠杆菌ＭＬ－３５ｐ耐药株都有杀菌活性。进一步采用电泳凝胶琼脂糖弥散法杀菌试验分析，结果表明大鼠膀胱粘膜酸溶性提取物中有两条蛋白带具明显的杀菌活性，我们称这两条蛋白带为ＲａｔＢＰ－１和ＲａｔＢＰ－２。而兔膀胱粘膜酸溶性提取物的杀菌活性亦与两条被称为ＲａｂＢＰ－１和ＲａｂＢＰ－２的蛋白带相关。本文首次提示，在膀胱粘膜内存在抗菌蛋白，可能是膀胱粘膜杀菌作用的分子基础。     

Effect of earth's orbital chirality on elementary particles and unification of chiral asymmetries in life on different levels

Life is chirally asymmetric at all scales from microscopic elementary particles to molecular and macroscopic levels. How these chiral asymmetries in life on different levels are unified remains unanswered. It has been demonstrated that both the biomolecular homochirality and biological rhythms can be caused by the right-handed helical force-field of the Earth's orbital chirality (EOC). Similar to the helical biomolecules (1), it is here suggested that the right-handed EOC force-field could make the right-handed elementary particles more stable than their left-handed enantiomers to result in the symmetry violation of elementary particles, and the EOC could also cause the macroscopic predominant selection of right-handed asymmetries of living objects (e.g. the helical seashells and plants). Our studies indicated that the weak force in weak interaction may only be a form of the EOC force-field at the microscopic particle level, and the chiral asymmetries in life on various levels could be unified by the natural right-handed EOC force-field. Moreover, the chiral and quantum effects, time, mass, rhythms and relativity could also be unified by the interaction of the EOC force-field with chiral motions and structures under certain conditions.     

Ha-VP39 binding to actin and the influence of F-actin on assembly of progeny virions

Summary We present evidence that actin is necessary for the successful assembly of HaNPV virions. Purified nucleocapsid protein Ha-VP39 of Heliothis armigera nuclear polyhedrosis virus (HaNPV) was found to be able to bind to actin in vitro without assistance, as demonstrated by Western blot and isothermal titration calorimeter. H and binding constants (K) detected by isothermal titration calorimeter strongly suggested that Ha-VP39 first binds actin to seed the formation of hexamer complex of actin, and the hexamers then link to each other to form filaments, and the filaments finally twist into cable structures. The proliferation of HaNPV was completely inhibited in Hz-AM1 cells cultivated in the medium containing 0.5µg/ml cytochalasin D (CD) to prevent polymerization of actin, while its yield was reduced to 10^–4 in the presence of 0.1µg/ml CD. Actin concentration and the viral DNA synthesis were not significantly affected by CD even though the progeny virions assembled in the CD treated cells were morphologically different from normal ones and resulted in fewer plaques in plaque assayThe authors equally contributed to the work.The authors equally contributed to the work.     

伯氏疟原虫对青蒿素抗药性的研究

仿Peters剂量递增法用伯氏疟原虫ANKA株及N株对QHS进行了抗药性的研究。经14个月的培育至第58代,QHS im注射“4日抑制性实验”的ED₅₀在RQ/ANKA系及RQ/N系分别为其亲代系的53.4及54.6倍,但经蚊传未获成功。在第40代(I₅₀=25)时,其50%的治愈剂量为其亲代系的5.4倍。停药传代其抗性会逐渐消失。该虫系对青蒿酯钠及蒿甲醚有明显的交叉抗性,其ED₅₀分别为其亲代系的13.1及11.7倍,对伯喹的抗性为2.9倍,对氯喹未见明显交叉抗性。     

VALUE AND LIMITATIONS OF SINUS NODE FUNCTION TE.ST BY ATRIAL PACING IN SICK SINUS SYNDROME DIAGNOSIS

In order to evaluate the significance of the sinus node function test by transvenous atrial pacing in the diagno,sis of sick sinus syndrome (SSS), sinus node recovery time (SRT), co]rrected sinus node recovery time (CSRT) and total atrio- sinus and sino-atrial conduction time (SACT) are observed in 69 patients including non-SSS group 39 cases and SSS group 30 cases. According to our data, we suggest criteria for sinus node dysfunction as follows. SRT>1,400 ms and/or A-V junctional esca.pe before sinus recovery and;or secondary pause, CSRT>560 ms. total SACT>300 ms. With l item abnormal, the false positive rat.e in the non-SSS group is 7.7c7。 and the false negative rate in the S.SS group 3.30i" with 2 items abnormal, the false positive rate in the non-SSS group Oi and the false negative rate in the SSS grOiup 6.7'70. Atropine test results are compared with that of atrial pacing, 95.8To and 84.2% are compatible with each other in these 2 groups. In order to cut the use of invasive technic to the minimum, we propose performing the atropine test first. Only in those with unexplainable test results should the trial pacing test. be resorted to..     

经典名方中杜仲的本草考证

通过查阅历代本草、医籍、方书并结合近现代文献资料,笔者对杜仲药材的名称、基原、产地、品质评价、采收加工、炮制历史沿革及产地变迁情况进行了系统梳理及考证,以期为含杜仲的经典名方开发提供参考依据。经考证可知,历代本草均以杜仲为正名,基原为杜仲科植物杜仲Eucommia ulmoides的干燥树皮,古今一致;杜仲最早的产地为河南、山西、陕西、四川一带,自明代以来产地扩展至全国大部分地区,且推崇四川、陕西、重庆、贵州、湖北等地为杜仲的道地产区;近代以来总结其品质以皮厚、块大、粗皮刮净、断面多丝、内表面色暗紫为佳;杜仲的古代炮制加工方法主要有去粗皮切制生用和加酥蜜、姜汁、盐水、酒等辅料炮制,近现代以来炮制方法日趋简化,沿用的炮制方法主要为净制后切制生用、盐炙,建议挖掘不同杜仲炮制品的现代科学内涵,通过标准恢复传统主流炮制方法。基于宋代陈自明的三痹汤中杜仲“去皮,切,姜汁炒”的要求,根据考证结果,建议使用姜杜仲,即参考2020年版《中华人民共和国药典》炒法,以姜汁为辅料进行炮制后入药。     

抗菌药物对骨科围术期预防感染的影响

目的探究抗菌药物对骨科围术期预防感染的影响。方法选择2016年1月-2019年1月瑞安市人民医院骨科收治的手术患者82例,随机数表法分为对照组和研究组,每组各41例,其中对照组围术期给予常规应用抗菌药物,研究组围术期给予干预性应用抗菌药物,观察两组抗菌药物使用情况、抗菌药物费用,并对抗菌药物使用的合理性进行评价。结果研究组住院时间、手术部位感染、治疗各项费用均低于对照组,差异有统计学意义(P<0.05);两组体温异常情况比较,无统计学差异;两组围术期抗菌药物使用均主要以单一用药为主,研究组单一用药多于对照组,二联用药低于对照组,差异有统计学意义(P<0.05);研究组在抗菌药物品种选择、预防用药时机、术后用药时间等围术期抗菌药物使用合理率均高于对照组,差异有统计学意义(P<0.05);两组治疗前肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、IL-8、C-反应蛋白(CRP)各项血清炎性因子水平比较,无统计学差异;治疗后,两组血清炎性因子水平均下降,差异有统计学意义(P<0.05)。结论干预性应用抗菌药物对骨科围术期预防感染效果显著,在降低手术部位感染发生率的同时,提高抗菌药物使用合理性、缩短住院时间、降低住院费用。