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排序方式: 共有438条查询结果,搜索用时 15 毫秒
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GN Dalekos ; E Zervou ; F Karabini ; M Elisaf ; K Bourantas ; KC Siamopoulos 《Transfusion》1995,35(6):503-506
BACKGROUND: In addition to human immunodeficiency virus, human T- lymphotropic virus types I and II (HTLV-I/II) is prevalent among blood donors in the United States. In Greece, there are no epidemiologic data regarding the prevalence of HTLV-I/II among volunteer blood donors and high-risk groups. STUDY DESIGN AND METHODS: To determine the prevalence of HTLV-I/II infections in northwestern Greece, a seroepidemiologic study was conducted among volunteer blood donors, multiply transfused patients, heroin addicts, and chronic hemodialysis patients. The subjects were tested for serologic evidence of HTLV-I/II infection by enzyme immunoassays and specific protein immunoblot confirmatory test. RESULTS: None of the volunteer blood donors and heroin addicts had detectable antibodies to HTLV-I/II. Only 1 (1.45%) of the 69 multiply transfused patients had indeterminate results, while 2 (1.2%) of 163 hemodialysis patients were positive. CONCLUSION: In northwestern Greece, routine screening for HTLV-I and HTLV-II infections does not appear to be required. However, the finding of seropositivity among hemodialysis patients requires further evaluation of the origin of the infection, as its zero prevalence in this population seems to exclude transfusion transmission. 相似文献
94.
Identification, chromosomal mapping and tissue-specific expression of hREV3 encoding a putative human DNA polymerase zeta 总被引:5,自引:0,他引:5
Xiao W; Lechler T; Chow BL; Fontanie T; Agustus M; Carter KC; Wei YF 《Carcinogenesis》1998,19(5):945-949
The Saccharomyces cerevisiae REV3 gene encodes the catalytic subunit of a
non-essential DNA polymerase zeta, which is required for mutagenesis. The
rev3 mutants significantly reduce both spontaneous and DNA damage- induced
mutation rates. We have identified human cDNA clones from two different
libraries whose deduced amino acid sequences bear remarkable homology to
the yeast Rev3, and named this gene hREV3. The hREV3 gene was mapped to
chromosome 1p32-33 by fluorescence in situ hybridization. The hREV3 encodes
an mRNA of >10 kb, and its expression varies in different tissues and
appears to be elevated in some but not all of the tumor cell lines we have
examined. In light of recent reports of a putative mouse REV3, these
results indicate that mammalian cells may also contain a mutagenic pathway
which aids in cell survival at the cost of increased mutation.
相似文献
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Novel targets for sensitizing breast cancer cells to TRAIL‐induced apoptosis with siRNA delivery 下载免费PDF全文
Bindu Thapa Remant Bahadur KC Hasan Uludağ 《International journal of cancer. Journal international du cancer》2018,142(3):597-606
Tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) induces apoptosis in variety of cancer cells without affecting most normal cells, which makes it a promising agent for cancer therapy. However, TRAIL therapy is clinically not effective due to resistance induction. To identify novel regulators of TRAIL that can aid in therapy, protein targets whose silencing sensitized breast cancer cells against TRAIL were screened with an siRNA library against 446 human apoptosis‐related proteins in MDA‐231 cells. Using a cationic lipopolymer (PEI‐αLA) for delivery of library members, 16 siRNAs were identified that sensitized the TRAIL‐induced death in MDA‐231 cells. The siRNAs targeting BCL2L12 and SOD1 were further evaluated based on the novelty and their ability to sensitize TRAIL induced cell death. Silencing both targets sensitized TRAIL‐mediated cell death in MDA‐231 cells as well as TRAIL resistant breast cancer cells, MCF‐7. Combination of TRAIL and siRNA silencing BCL2L12 had no effect in normal human umbilical vein cells and human bone marrow stromal cell. The silencing of BCL2L12 and SOD1 enhanced TRAIL‐mediated apoptosis in MDA‐231 cells via synergistically activating capsase‐3 activity. Hence, here we report siRNAs targeting BCL2L12 and SOD1 as a novel regulator of TRAIL‐induced cell death in breast cancer cells, providing a new approach for enhancing TRAIL therapy for breast cancer. The combination of siRNA targeting BCL2L12 and TRAIL can be a highly effective synergistic pair in breast cancer cells with minimal effect on the non‐transformed cells. 相似文献
97.
Swaroop Kumar Sahu MB Soudarssanane Gautam Roy KC Premrajan Sonali Sarkar 《Indian Journal of Community Medicine》2008,33(2):81-84
Portfolio-based learning is recognized in medical education. It helps students to assess themselves as per the key learning objectives and outcomes expected out of them. The faculty could also get feedback regarding individual student''s progress toward learning outcomes and facilitate the students achieve the same. This article addresses the process of portfolio development and assesses from students feedbacks, if portfolio-based learning is an improvement over record-based study in community-based field studies. The results of this study shows that involving students in framing objectives, developing a mechanism for self-introspection and self-assessment by the students and a mechanism by which faculty can monitor each student''s progress toward the defined objectives can significantly enhance the learnability of the students. 相似文献
98.
Treatment of lipomas assisted with tumescent liposuction 总被引:1,自引:0,他引:1
CW Choi† BJ Kim‡ SE Moon SW Youn KC Park CH Huh 《Journal of the European Academy of Dermatology and Venereology》2007,21(2):243-246
BACKGROUND: Lipoma is a common soft-tissue tumour of mature fat cells. Although surgical excision is effective, treatments that are less invasive and not associated with disfigurement of scar would be ideal for the treatment of lipomas. Recently, tumescent liposuction has been used for the treatment of lipomas. OBJECTIVE: To evaluate the efficacy of tumescent liposuction in lipoma treatment, we reviewed our experience of lipoma treatment by tumescent liposuction. METHODS: A total of 21 patients presenting with 31 lipomas were treated with tumescent liposuction. After liposuction, remaining stromas were removed by a haemostat through the small incision. Tumour size and post-operative complications were recorded before and after treatment. RESULTS: A total of 31 lipomas of 21 patients were treated by tumescent liposuction. The size of lipomas ranged between 1.2 and 11 cm (mean size, 4.1 cm). In 23 cases, there were no complications. However, remnant lipomas, bruise, haematoma and immediate dimpling were found as complications. CONCLUSION: Tumescent liposuctions with extracting remnant fat tissue and fibrous tissue through the opening for liposuction can be an effective treatment technique in lipoma treatment in the efficacy and cosmetic outcomes and this method can be a substitute for excision in treating large lipomas. 相似文献
99.
P Mahesh Shanmugam Rajesh Ramanjulu KC Divyansh Mishra 《Indian journal of ophthalmology》2015,63(1):69-70
Wide field fundus imaging is needed to diagnose, treat, and follow-up patients with retinal pathology. This is more applicable for pediatric patients as repeated evaluation is a challenge. The presently available imaging machines though provide high definition images, but carry the obvious disadvantages of either being costly or bulky or sometimes both, which limits its usage only to large centers. We hereby report a technique of fundus imaging using a nasal endoscope coupled with viscoelastic. A regular nasal endoscope with viscoelastic coupling was placed on the cornea to image the fundus of infants under general anesthesia. Wide angle fundus images of various fundus pathologies in infants could be obtained easily with readily available instruments and without the much financial investment for the institutes. 相似文献
100.