全文获取类型
收费全文 | 1271篇 |
免费 | 48篇 |
国内免费 | 81篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 73篇 |
妇产科学 | 9篇 |
基础医学 | 139篇 |
口腔科学 | 23篇 |
临床医学 | 222篇 |
内科学 | 306篇 |
皮肤病学 | 33篇 |
神经病学 | 27篇 |
特种医学 | 300篇 |
外科学 | 58篇 |
综合类 | 27篇 |
预防医学 | 46篇 |
眼科学 | 6篇 |
药学 | 71篇 |
肿瘤学 | 59篇 |
出版年
2022年 | 5篇 |
2021年 | 8篇 |
2019年 | 13篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2016年 | 10篇 |
2015年 | 18篇 |
2014年 | 11篇 |
2013年 | 26篇 |
2012年 | 15篇 |
2011年 | 18篇 |
2010年 | 49篇 |
2009年 | 45篇 |
2008年 | 25篇 |
2007年 | 62篇 |
2006年 | 19篇 |
2005年 | 28篇 |
2004年 | 13篇 |
2003年 | 15篇 |
2002年 | 21篇 |
2001年 | 16篇 |
2000年 | 22篇 |
1999年 | 25篇 |
1998年 | 93篇 |
1997年 | 88篇 |
1996年 | 109篇 |
1995年 | 84篇 |
1994年 | 87篇 |
1993年 | 74篇 |
1992年 | 21篇 |
1991年 | 30篇 |
1990年 | 27篇 |
1989年 | 32篇 |
1988年 | 36篇 |
1987年 | 27篇 |
1986年 | 34篇 |
1985年 | 32篇 |
1984年 | 13篇 |
1983年 | 21篇 |
1982年 | 20篇 |
1981年 | 21篇 |
1980年 | 17篇 |
1979年 | 4篇 |
1978年 | 9篇 |
1977年 | 14篇 |
1976年 | 13篇 |
1975年 | 7篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有1400条查询结果,搜索用时 15 毫秒
991.
992.
993.
JP Fillastre S. Geffroy-Josse I. Etienne M. Dhib P. Rosenzweig P. Danjou C. Dubruc and G. Bianchetti 《Fundamental & clinical pharmacology》1993,7(1):1-9
Summary— Zolpidem, an imidazopyridine derivative, is a chemically novel, non-benzodiazepine hypnotic agent. Many uraemic patients complain of sleep disorders and ask for hypnotic medication which is well tolerated both clinically and biologically in such patients. We studied the pharmacokinetics and pharmacodynamics of zolpidem in 12 end-stage renal patients regularly treated by hemodialysis three times a week. Zolpidem (10 mg) was given orally for 14 or 21 days. Pharmacokinetic and pharmacodynamic evaluations were repeated at the end of the study on day 14 or day 21. Cmax , Tmax , t1/2 and the area under the curve were not modified in hemodialyzed patients. After daytime dosing, zolpidem induced the same level of sleepiness after the first and last dose and was well tolerated as a hypnotic agent after the night-time dosing. From these results, it can be said that zolpidem may be administered safely to patients with severe renal impairment without any modification of the dosage regimen. 相似文献
994.
A retrospective clinicopathological study of 100 necropsy cases of lung carcinoma revealed three scar cancers. The scarring in a further 11 probably occurred secondary to the tumour. The premise that lung scars initiate malignancy is questioned. 相似文献
995.
996.
The change in red blood cell membrane phosphate concentration of standard CPD whole blood stored in Fenwal blood bags at 4 C was measured daily for two weeks. Membrane phosphate concentrations increased rapidly when stored pH fell to 6.95. At the same time, the rate of K+ leakage from the cells increased, and transport of inorganic phosphate across the membrane decreased. It is concluded that gross uptake of phosphorus by the red blood cell membrane during blood bank storage may be in part responsible for physical changes in the membrane. 相似文献
997.
998.
Els van Riel Margreet GEM Ausems Frans BL Hogervorst Irma Kluijt Marielle E van Gijn Jeanne van Echtelt Karen Scheidel-Jacobse Eric FAM Hennekam Rein P Stulp Yvonne J Vos G Johan A Offerhaus Fred H Menko Johan JP Gille 《Hereditary cancer in clinical practice》2010,8(1):1-9
Background
An unclassified variant (UV) in exon 1 of the MLH1 gene, c.112A > C, p.Asn38His, was found in six families who meet diagnostic criteria for Lynch syndrome. The pathogenicity of this variant was unknown. We aim to elucidate the pathogenicity of this MLH1 variant in order to counsel these families adequately and to enable predictive testing in healthy at-risk relatives.Methods
We studied clinical data, microsatellite instability and immunohistochemical staining of MMR proteins, and performed genealogy, haplotype analysis and DNA testing of control samples.Results
The UV showed co-segregation with the disease in all families. All investigated tumors showed a microsatellite instable pattern. Immunohistochemical data were variable among tested tumors. Three families had a common ancestor and all families originated from the same geographical area in The Netherlands. Haplotype analysis showed a common haplotype in all six families.Conclusions
We conclude that the MLH1 variant is a pathogenic mutation and genealogy and haplotype analysis results strongly suggest that it is a Dutch founder mutation. Our findings imply that predictive testing can be offered to healthy family members. The immunohistochemical data of MMR protein expression show that interpreting these results in case of a missense mutation should be done with caution. 相似文献999.
Human platelet activation in the absence of aggregation: a calcium- dependent phenomenon independent of thromboxane formation 总被引:2,自引:0,他引:2
In response to ionophore A 23187, thrombasthenic and EDTA-treated control platelet-rich plasmas (PRP) undergo a change in light transmission (LT) accompanied by a normal 14C-serotonin (5HT) release and thromboxane (TX) synthesis in the absence of aggregation. Ultrastructural qualitative electron microscopy revealed central apposition of organelles and loosely packed platelets in both models, while a central gel mass appeared only in thrombasthenic patients. Quantitative analysis of this ultrastructural change showed an increase in the elongation and a decrease in the circularity coefficients of thrombasthenic platelets, indicating a shape change with pseudopod formation, while EDTA-treated platelets underwent a shape change in the absence of pseudopod formation. Morphometric analysis showed that the ionophore caused extensive degranulation in both types of platelets (decrease of the granule volume), which occurred in the presence of contraction of thrombasthenic PRP (decrease of the SCS plus granule volume) but in its absence in EDTA-treated platelets. The change in LT was not inhibited by aspirin, suggesting a dissociation between release of 14C-5HT and TX formation. Moreover, it was not inhibited by creatine phosphate plus creatine phosphokinase, prostaglandin E1, or cytochalasin and/or colchicine. It was not dependent on ADP, cAMP, or the integrity of microfilaments and microtubules. However, chlorpromazine, TMB 8, and dibucaine, which interfere with intracellular membrane transport of Ca2+, inhibited this platelet activation (change in LT, 14C-5HT release and TX synthesis. 相似文献
1000.
One‐year metreleptin improves insulin secretion in patients with diabetes linked to genetic lipodystrophic syndromes 下载免费PDF全文
C. Vatier S. Fetita P. Boudou C. Tchankou L. Deville JP. Riveline J. Young L. Mathivon F. Travert D. Morin J. Cahen O. Lascols F. Andreelli Y. Reznik E. Mongeois I. Madelaine MC. Vantyghem JF. Gautier C. Vigouroux 《Diabetes, obesity & metabolism》2016,18(7):693-697
Recombinant methionyl human leptin (metreleptin) therapy was shown to improve hyperglycaemia, dyslipidaemia and insulin sensitivity in patients with lipodystrophic syndromes, but its effects on insulin secretion remain controversial. We used dynamic intravenous (i.v.) clamp procedures to measure insulin secretion, adjusted to insulin sensitivity, at baseline and after 1 year of metreleptin therapy, in 16 consecutive patients with lipodystrophy, diabetes and leptin deficiency. Patients, with a mean [± standard error of the mean (s.e.m.)] age of 39.2 (±4) years, presented with familial partial lipodystrophy (n = 11, 10 women) or congenital generalized lipodystrophy (n = 5, four women). Their mean (± s.e.m.) BMI (23.9 ± 0.7 kg/m2), glycated haemoglobin levels (8.5 ± 0.4%) and serum triglycerides levels (4.6 ± 0.9 mmol/l) significantly decreased within 1 month of metreleptin therapy, then remained stable. Insulin sensitivity (from hyperglycaemic or euglycaemic‐hyperinsulinaemic clamps, n = 4 and n = 12, respectively), insulin secretion during graded glucose infusion (n = 12), and acute insulin response to i.v. glucose adjusted to insulin sensitivity (disposition index, n = 12), significantly increased after 1 year of metreleptin therapy. The increase in disposition index was related to a decrease in percentage of total and trunk body fat. Metreleptin therapy improves not only insulin sensitivity, but also insulin secretion in patients with diabetes attributable to genetic lipodystrophies. 相似文献