HPLC测定微量小鼠血浆中对乙酰氨基酚的浓度

建立了一种高效液相以谱法测定微量小鼠血浆中对乙酰氨基酚的浓度，采用非那西丁为内标，该法在５．００－１５０．０μｇ／ｍｌ范围内线性相关，方法回收率在９５％以上，日间日内相对标准偏差小于３％，与对乙酰氨基酚相关的６种药物对测定无干扰。     

Chronic osteomyelitis of the mandible following tooth extraction in HIV infection

A case is presented of refractory chronic osteomyelitis of the left mandible arising after a tooth extraction in HIV infection. The challenges of prompt diagnosis and the dilemma of satisfactory management are highlighted. It is imperative that dental and oral health providers have a particularly high index of suspicion of conditions that occur in HIV infection so that early identification and appropriate management may be instituted.     

2,4-Disubstituted thiazoles II. A novel class of antitumor agents, synthesis and biological evaluation

A series of 2,4-disubstituted thiazole derivatives bearing N-n-butyl or N-cyclohexylthioureido synthon at position 2 and N-substituted thiosemicarbazone moiety at position 4 have been synthesized and tested for antitumor activity using the National Cancer Institute's in-vitro-disease-oriented antitumor screen. All of the tested compounds showed antineoplastic activity at concentrations less than 100 μM. Compounds 7, 9, 15 and 17 in particular showed activity with GI₅₀ (mean-graph midpoint) of 17.8, 8.5, 9.5 and 7.4 μM, respectively. The detailed syntheses, spectroscopic and biological data are discussed.     

CT-guided aspirations for the body: comparison of hand guidance with stereotaxis

Forty computed tomography (CT)-assisted aspirations performed with only hand guidance were prospectively compared with 40 performed with a CT body-stereotaxic system. Although there was no statistically significant difference in lesion size and path length between the two groups, use of stereotaxis compared with hand guidance decreased by 75% the number of needle manipulations required to place a needle within a lesion. With the stereotaxic method, only 43 needle manipulations were required to confirm a needle placement in 40 lesions, with no lesion requiring more than two attempts. Use of stereotaxis decreased the number of localization scans by 80% and biopsy time by 50%. It is concluded that CT-guided needle placements with hand guidance are often inaccurate and, unless the lesion is large, require multiple needle manipulations to place a needle within the lesion. Stereotaxis-guided biopsies, on the other hand, decrease radiation exposure, biopsy time, and trauma from multiple needle punctures.     

hp-MSCs对RSV引起小儿毛细支气管炎的保护作用及机制研究

目的 探讨人胎盘间充质干细胞（hp-MSCs）水平降低与毛细支气管炎发病的相关性及hP-MSCs防治毛细支气管炎的分子机制。方法 在滨州医学院附属医院产科招募志愿者,同时收集新生儿胎盘。毛细支气管炎患者由儿科医生根据临床诊断标准做出诊断;以毛细支气管炎患者为病例组,正常体检儿童为对照组,进行病例对照研究;胎盘hp-MSCs水平检测采用锥虫蓝染色计数法,hp-MSCs表面标记物的检测采用流式细胞仪方法;采用ELISA（酶联免疫吸附法）检测白细胞介素-9(IL-9)、白细胞介素-17(IL-17）、白细胞介素 10(IL-10）和转化生长因子 -1(TGFβ-1）水平,采用Real time PCR和Western blot法检测叉头框蛋白3(Foxp3）及维甲酸相关孤核受体γt(RORγt）基因和蛋白表达水平。结果 两组人群在是否存在特异性皮炎、毛细支气管炎1级和2级家族史有显著性差异（P＜0.05）。病例组hp-MSCs水平及其表面标记物CD44和CD29水平显著降低（P＜0.05）。呼吸道合胞体病毒（RSV）感染升高IL-17和IL-9等炎症因子水平,降低IL-10和TGFβ-1等抗炎因子水平;而hp-MSCs干预可以降低IL-17和IL-9水平而升高IL-10和TGFβ-1水平（均P＜0.05）。RSV感染可以下调Foxp3基因和蛋白表达,上调RORγt基因和蛋白表达;而hp MSCs干预可以上调Foxp3基因和蛋白表达,下调RORγt基因和蛋白表达。结论 胎盘hp MSCs水平降低是引起婴幼儿毛细支气管炎的独立危险因素,hp-MSCs通过上调Foxp3的蛋白和基因表达,以及下调RORγt蛋白和基因表达起到防治婴幼儿毛细支气管炎的作用。