Correlation of radiation tolerance dose of normal human organs with organ weight, blood, and water content

The concerted effort to minimize the radiation exposure to normal human tissues while delivering a high radiation dose to the tumor often results in complications. This limits the efficacy of radiation treatment. Analysis of radiation tolerance dose with organ weight in 15 human organs yields a correlation coefficient of 0.62, whereas the correlation of radiation tolerance dose with blood and water content yields correlation coefficients of 0.82 and 0.60, respectively. Results indicate that as the organ weight and/or blood and water content increases, radiation tolerance dose decreases.     

降糖孜亚比提片对糖尿病小鼠肝脏的影响

目的研究降糖孜亚比提片对糖尿病肝脏的降糖作用。方法采用四氧嘧啶糖尿病小鼠模型对降糖孜亚比提片进行研究。结果对四氧嘧啶引起的高血糖小鼠的血糖有明显的抑制作用;增加肝糖原的含量,减少肝糖原的输出;并且保护肝脏。结论降糖孜亚比提片具有改善糖尿病肝脏的作用。     

Effects of trypsin-induced limited hydrolysis on the structural,functional, and bioactive properties of sericin

The effects of trypsin-induced hydrolysis on the structural, functional, and antioxidant properties of sericin were studied. The structural properties of sericin and its hydrolysates were characterized by using SDS-PAGE, SEC-HPLC, surface hydrophobicity, and circular dichroism. Antioxidative properties were evaluated based on quenching capacity against hydroxyl, DPPH, and ABTS, and metal (Fe²⁺, Cu²⁺) chelating activity. The enzymatic hydrolysis raised the flexibility, changed emulsifying and foaming properties, and improved the solubility and antioxidant activity of sericin. Meanwhile, the hydrolysis led to a decline in gelation capacity, oil holding capacity, and water holding capacity. Sericin and its hydrolysates exhibited excellent function with regard to oil holding, emulsifying, and foaming. Sericin and its hydrolysates had clear effects on the growth of both Enterococcus faecalis and Lactobacillus bulgaricus strains.

Sericin was hydrolyzed to a limited extent using trypsin, then the physicochemical, prebiotic, and antioxidative properties of the obtained hydrolysates were investigated.     

基于HPLC指纹图谱结合化学模式识别法和薄层色谱生物自显影评价不同生长期毛菊苣药材质量

目的 建立不同生长期(花前期、开花期和成熟期)毛菊苣Cichorium glandulosum地上部位药材的HPLC指纹图谱,结合化学模式识别法和薄层色谱(TLC)生物自显影技术评价毛菊苣药材质量,为其进一步研究与开发提供依据。方法 采用PolyPack C₁₈-AQ色谱柱,以甲醇(A)-乙腈(B)-0.3%磷酸溶液(C)为流动相进行梯度洗脱,检测波长为254 nm,体积流量为1.0 mL/min。采用《中药色谱指纹图谱相似度评价系统(2012)》建立不同生长期毛菊苣地上部位药材的HPLC指纹图谱并分析相似度。应用聚类分析(cluster analysis,CA)、主成分分析(principal component analysis,PCA)和偏最小二乘法-判别分析(partial least squares-discriminant analysis,PLS-DA)筛选不同生长期下毛菊苣地上部位药材中具有的差异性化学成分。利用TLC-生物自显影技术对不同生长期毛菊苣药材进行体外抗氧化作用研究。结果 不同生长期下20批次药材HPLC指纹图谱共匹配出12个共有峰,指认出3号峰为秦皮乙素、6号峰为异槲皮苷、7号峰为3,5-O-二咖啡酰奎宁酸。CA将20批不同生长期下药材分为4类;PCA确定了3个主成分因子,累积方差贡献率为78.143%,PCA结果与CA结果基本一致;PLS-DA结果显示色谱峰11、2、6(异槲皮苷)、3(秦皮乙素)和5号峰可作为不同生长期毛菊苣药材质量的差异标志物。样品展开斑点和TLC-生物自显影说明花前期和开花期药材相比成熟期药材具有良好的抗氧化活性作用。结论 利用建立的指纹图谱可为毛菊苣地上部位药材规范化种植以及质量评价提供实验依据。     

降糖孜亚比提片降血糖作用的实验研究

目的：研究降糖孜亚比提片的降糖作用。方法：采用正常动物及糖尿病模型动物对降糖孜亚比提片的降糖作用进行研究。结果：降糖孜亚比提片对正常小鼠血糖无影响；能够提高小鼠的糖耐量；对四氧嘧啶引起的高血糖小鼠的血糖有明显的抑制作用；还能使肾上腺素所致的高血糖小鼠血糖降低。结论：降糖孜亚比提片具有明显的降血糖作用。     

毛菊苣种子质量标准研究

目的 探讨毛菊苣种子药材的鉴别方法,完善毛菊苣种子的药材质量标准.方法 采用薄层色谱法,对毛菊苣种子中活性成分秦皮乙素进行鉴别;以高效液相色谱法测定秦皮乙素含量.选用Phenomenex Luna C_(18)柱(250 mm×4.6 mm, 5 μm),流动相为甲醇-0.3%甲酸(25∶75), 流速1.0 ml·min~(-1),检测波长349 nm,进样量10 μl.结果 薄层色谱中秦皮乙素斑点清晰,无干扰.高效液相色谱法精密度、重复性良好;秦皮乙素在0～0.2 μg范围内有较好的线性关系,3批药材中秦皮乙素含量分别为0.099 7,0.110 9,0.097 2 mg·g~(-1).结论 该方法稳定、可靠,为评价毛菊苣种子药材的质量提供科学依据.     

Gossypium herbaceam extracts inhibited NF-kappaB activation to attenuate spatial memory impairment and hippocampal neurodegeneration induced by amyloid-beta in rats

Amyloid-beta (Abeta) is considered to be responsible for the pathogenesis of Alzheimer's disease. In the present study, the protective effect of Gossypium herbaceam extracts (GHE) on learning and memory impairment induced by Abeta were examined in vivo using Morris water maze and step through task. Furthermore, the antioxidant activity and neuroprotective effect of GHE was investigated with methods of histochemistry and biochemistry. These data showed that oral administration with GHE at the doses of 35, 70 and 140 mg/kg exerted an improved effect on the learning and memory impairment in rats induced by intracerebroventricular (i.c.v.) injection of 10 microg of Abeta(25-35). Subsequently, the GHE afforded a beneficial action on promotion on the activity of glutathione peroxidase and catalase, as well as inhibition on the NF-kappaB activation in the hippocampus followed by the presence of Abeta(25-35). Meanwhile, the number of degenerating neurons with an apoptotic feature was dramatically decreased in hippocampus after treatment with GHE, implicating that its antioxidant stress and inhibition of NF-kappaB activation could be involved in the mechanism underlying neuroprotection of GHE against Abeta-induced cell death. These findings suggested that GHE might be a potential agent for treatment of Alzheimer's disease.