全文获取类型
收费全文 | 811篇 |
免费 | 47篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 21篇 |
妇产科学 | 14篇 |
基础医学 | 103篇 |
口腔科学 | 27篇 |
临床医学 | 36篇 |
内科学 | 223篇 |
皮肤病学 | 10篇 |
神经病学 | 57篇 |
特种医学 | 74篇 |
外科学 | 136篇 |
综合类 | 9篇 |
预防医学 | 12篇 |
眼科学 | 10篇 |
药学 | 27篇 |
中国医学 | 2篇 |
肿瘤学 | 95篇 |
出版年
2021年 | 10篇 |
2020年 | 7篇 |
2019年 | 6篇 |
2018年 | 13篇 |
2017年 | 7篇 |
2016年 | 9篇 |
2015年 | 16篇 |
2014年 | 17篇 |
2013年 | 22篇 |
2012年 | 36篇 |
2011年 | 40篇 |
2010年 | 28篇 |
2009年 | 28篇 |
2008年 | 21篇 |
2007年 | 28篇 |
2006年 | 31篇 |
2005年 | 26篇 |
2004年 | 27篇 |
2003年 | 24篇 |
2002年 | 36篇 |
2001年 | 33篇 |
2000年 | 33篇 |
1999年 | 41篇 |
1998年 | 25篇 |
1997年 | 20篇 |
1996年 | 17篇 |
1995年 | 12篇 |
1994年 | 9篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 11篇 |
1989年 | 9篇 |
1988年 | 26篇 |
1987年 | 22篇 |
1986年 | 16篇 |
1985年 | 12篇 |
1984年 | 9篇 |
1983年 | 14篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 9篇 |
1978年 | 4篇 |
1977年 | 7篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1969年 | 5篇 |
1967年 | 6篇 |
1966年 | 8篇 |
排序方式: 共有861条查询结果,搜索用时 265 毫秒
11.
12.
To investigate the pathogenesis of brain abnormalities caused by congenital cytomegalovirus (CMV) infection, we previously reported experimental murine models of brain damage induced by intraventricular injection of murine CMV (MCMV) at the late stage of gestation. In the present study, viral DNA-positive cells in the damaged brain at different postnatal stages detected by in situ hybridization were compared with viral antigen-positive cells detected by an immunoperoxidase method using a monoclonal antibody against the immediate early antigen. At birth, the number of viral DNA-positive cells almost equalled that of viral antigen-positive cells. Seven to ten days after birth, the number of viral DNA-positive cells in the brain of MCMV-injected mice was one-fifth that of viral antigen-positive cells. Viral DNA-positive cells were more numerous in the hippocampus than in the cortex, and their density was dependent on the presence of viral antigen-positive cells. Dotted reaction products were observed in the nuclei of viral DNA-positive cells. These cells were rarely detected in lesions of later stages such as atrophy of the cortex and hippocampus, or the wall of the cystic lesions. These results suggest that viral DNA-positive cells detected by in situ hybridization are infected cells in which viral DNA replication is occurring actively. 相似文献
13.
Interchromosomal duplications of the adrenoleukodystrophy locus: a phenomenon of pericentromeric plasticity 总被引:13,自引:5,他引:13
Eichler EE; Budarf ML; Rocchi M; Deaven LL; Doggett NA; Baldini A; Nelson DL; Mohrenweiser HW 《Human molecular genetics》1997,6(7):991-1002
A 9.7 kb segment encompassing exons 7-10 of the adrenoleukodystrophy (ALD)
locus of the X chromosome has duplicated to specific locations near the
pericentromeric regions of human chromosomes 2p11,10p11, 16p11 and 22q11.
Comparative sequence analysis reveals 92-96% nucleotide identity,
indicating that the autosomal ALD paralogs arose relatively recently during
the course of higher primate evolution (5-10 million years ago). Analysis
of sequences flanking the duplication region identifies the presence of an
unusual GCTTTTTGC repeat which may be a sequence-specific integration site
for the process of pericentromeric- directed transposition. The breakpoint
sequence and phylogenetic analysis predict a two-step transposition model,
in which a duplication from Xq28 to pericentromeric 2p11 occurred once,
followed by a rapid distribution of a larger duplicon cassette among the
pericentromeric regions. In addition to facilitating more effective
mutation detection among ALD patients, these findings provide further
insight into the molecular basis underlying a pericentromeric-directed
mechanism for non- homologous interchromosomal exchange.
相似文献
14.
In cat hypoglossal motoneurons after axotomy the synaptic efficacy of inhibitory synapses made by the lingual nerve afferent fibers was studied. The amplitude of the short- and the long-lasting inhibitory postsynaptic potential produced in tongue protruder motoneurons 24 days after axotomy by stimulation of the lingual nerve was significantly reduced in size as compared with the control on the unoperated side. In most protruder motoneurons 40 days after axotomy a large excitatory postsynaptic potential and a spike was produced by stimulation of either the ipsilateral or the contralateral lingual nerve. We have demonstrated that the decline of synaptic efficacy of inhibitory synapses for the short-lasting inhibitory postsynaptic potential was more prominent than that for the long-lasting inhibitory potential in the motoneuron 24 days after axotomy. After the cut axons of protruder motoneurons were re-united to tongue muscles, we have demonstrated that the decline of synaptic efficacy of inhibitory synapses for the short-lasting inhibitory postsynaptic potential was less prominent than that in axotomized protruder motoneurons. 相似文献
15.
Possible role of autoantibodies against nephrin in an experimental model of chronic graft-versus-host disease 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Nagahama K Maru K Kanzaki S Chai HL Nakai T Miura S Yamaguchi A Yamanaka S Nagashima Y Aoki I 《Clinical and experimental immunology》2005,141(2):215-222
Nephrin, a product of the NPHS1 gene, is a component of the slit diaphragms that are found between glomerular foot processes and is a crucial element for glomerular filtration barrier. Recently, nephrin has been focused in a number of studies of proteinuria development including various types of acquired glomerular diseases including minimal change nephrotic syndrome and membranous nephropathy. However, the precise role of nephrin in such acquired glomerular diseases is still unknown. To analyse the role of nephrin further, two kinds of anti-nephrin antibodies were raised in the rabbits and applied to an experimental mouse model of chronic graft-versus-host disease, in which (C57BL/10 x DBA/2) F1 mice developed clinically apparent severe proteinuria with significant glomerular lesions 7 weeks after parental DBA/2 cell transfer. Antibody-sandwich ELISA detected anti-nephrin antibodies during week 2 to week 6, with the peak at week 2 or week 4. Colocalization of nephrin and IgG on week 4, week 6, and week 8 was revealed by confocal microscopic analysis, suggesting that in situ immune complex formation with nephrin in glomerular lesion. Taken together, it seems to be suggested nephrin and its autoantibody have a certain role in the development of glomerular lesion in our model mice. 相似文献
16.
Identity of hemolysins produced by Vibrio cholerae non-O1 and V. cholerae O1, biotype El Tor. 总被引:8,自引:11,他引:8
K Yamamoto Y Ichinose N Nakasone M Tanabe M Nagahama J Sakurai M Iwanaga 《Infection and immunity》1986,51(3):927-931
Hemolysins purified from non-O1 Vibrio cholerae (non-O1 hemolysin) and a Vibrio cholerae O1, biotype El Tor (El Tor hemolysin) were investigated for their homology. The hemolysins were isolated from the culture supernatant fluids by ammonium sulfate precipitation and gel filtration on Sephadex G-100 columns. The purified hemolysins gave single bands with an identical mobility on conventional polyacrylamide gel disc electrophoresis. The molecular weights of the non-O1 and El Tor hemolysins were estimated to be about 60,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the amino acid compositions of the hemolysins were very similar. The specific activities of the hemolysins were identical, and both hemolysins were neutralized to the same extent with antisera against the homologous and heterologous hemolysins. Ouchterlony double immunodiffusion tests with both hemolysins and antihemolysin serum gave a common (fused) precipitin line. These data indicate that the non-O1 hemolysin is biologically, physicochemically, and immunologically indistinguishable from the El Tor hemolysin. 相似文献
17.
Two types of phantoms were developed with which to evaluate the overall performance of digital subtraction angiography (DSA) systems. A dynamic phantom, called a "fish bone" phantom, consists of polyethylene tubes that simulate blood vessels with various lesions, such as stenoses, ulcers, and aneurysms. With this phantom, washout curves were obtained representing the relationship between iodine content and time. It will be useful for qualitative assessment of DSA images, evaluation of different image-processing schemes, and studies of blood flow analysis. A static phantom, called a "C-D" phantom, can be used for measurement of quantitative contrast-detail (C-D) diagrams and for daily monitoring of DSA systems. This was constructed of tubes of seven different diameters (2.15-0.28 mm) and 14 different concentrations of contrast medium (100%-1.1% Renografin-76 [meglumine and sodium diatrizoate]). The C-D diagrams were determined from an observer performance study using C-D phantom images obtained at four different DSA settings. 相似文献
18.
19.
20.