咖啡因5种主要代谢物的反相高效液相色谱法测定

为保证应用咖啡因代谢探针的正确性,建立了反相高效液相色谱测定尿中咖啡因代谢物的方法。采用日本岛津 Shim Pack CLC-ODS柱(5μm),以甲醇—乙腈—0.05%醋酸(12∶1∶87)为流动相,咖啡因和它的13种代谢物及内标均能良好分离。重点对其中5个主要代谢物AFMU,1U,1X,17U,17X进行了测定。结果表明这5种代谢物回收率均在87%以上,日内和日间误差均小于3%,显示了方法的稳定性。并对120例志愿者尿中5种主要代谢物浓度进行了测定,为进一步评价多种药物代谢酶活性创造条件。     

慢性肾炎患者外周血CD62P表达的临床意义

目的　探讨慢性肾炎患者外周血CD62P的表达同慢性肾炎进展的关系。方法　慢性肾炎患者 52例根据BUN、Cr分为Ⅰ组 :肾功能不全代偿期 (BUN≤ 9mmol/L ,Cr≤ 178μmol/L) 2 0例 ,Ⅱ组 :氮质血症期 (9mmol/L2 0mmol/L ,Cr >445μmol/L) 13例 ,用流式细胞仪分别测定 3组患者外周血CD62P的表达 ,并同正常对照组进行比较。结果　 3组外周血CD62P的表达分别为 9.12 %± 2 .5%、7.13 %± 2 .1%、3 .41%± 1.5% ,均高于正常对照组的 1.87%± 0 .65% (P <0 .0 1,P <0 .0 5) ,Ⅰ、Ⅱ组又显著高于Ⅲ组 (P <0 .0 1)。各组间血小板的常规指标比较无明显差异 (P >0 .0 5)。结论　慢性肾炎患者外周血CD62P的表达与肾脏病变的发生、发展有密切关系     

应用人骨髓间质干细胞与脱细胞猪主动脉瓣膜支架体外构建组织工程心脏瓣膜

目的:应用动物来源的细胞构建组织工程化心脏瓣膜的研究较多,本实验特征在于观察人骨髓间质干细胞与脱细胞猪主动脉瓣膜支架体外构建组织工程心脏瓣膜,并探讨其作为种子细胞的可行性。方法:实验于2005-12/2006-10在解放军第二军医大学长海医院全军胸心外科研究所完成,研究方案获伦理委员会批准。①实验材料:取开胸术中切除的肋骨骨髓,术前已经患者知情同意。新鲜猪心脏瓣膜取自上海复新屠宰场。②实验方法:Ficoll密度梯度离心从肋骨骨髓中获取骨髓单个核细胞,体外扩增培养后鉴定。采用去污剂和酶消化法将猪主动脉瓣叶行脱细胞处理,种植人骨髓间质干细胞。③实验评估:采用EnVision二步法染色鉴定人骨髓间质干细胞形态及细胞表型;采用苏木精-伊红染色、维多利亚蓝染色和VanGieson氏染色法观察细胞在脱细胞猪主动脉瓣叶支架上的生长特点;扫描电镜观察静态构建组织工程心脏瓣膜的形态。结果:①人骨髓间质干细胞形态及细胞表型鉴定:人骨髓间质干细胞为梭形,抗平滑肌抗体和波形蛋白染色阳性,CD31及Ⅷ因子染色阴性。②脱细胞猪主动脉瓣膜支架的组织学观察:猪去细胞瓣膜支架去细胞完全,弹力纤维及胶原纤维保持完整,种植的人骨髓间质干细胞在脱细胞瓣叶表面形成完整的细胞层。③构建组织工程心脏瓣膜的形态学观察:扫描电镜示瓣膜表面细胞层完整,细胞呈梭形复层生长。结论:人骨髓间质干细胞体外具有自然分化为肌成纤维细胞的特性,在去细胞猪主动脉瓣膜上生长良好,可作为一种有前途的种子细胞构建组织工程心脏瓣膜。     

Short and long term outcome of neonatal hyperglycemia in very preterm infants: a retrospective follow-up study

Background  

Hyperglycemia in premature infants is associated with increased morbidity and mortality, but data on long-term outcome are limited. We investigated the effects of neonatal hyperglycemia (blood glucose ≥ 10 mmol/l, treated with insulin for ≥ 12 hours) on growth and neurobehavioral outcome at 2 years of age.     

Novel diagnostic features of dysferlinopathies

Reports of dysferlinopathy have suggested a clinically heterogeneous group of patients. We identified specific novel molecular and phenotypic features that help distinguish dysferlinopathies from other forms of limb‐girdle muscular dystrophy (LGMD). A detailed history, physical exam, and protein and mutation analysis of genomic DNA was done for all subjects. Five of 21 confirmed DYSF gene mutations were not previously reported. A distinct “bulge” of the deltoid muscle in combination with other findings was a striking feature in all patients. Six subjects had atypical calf enlargement, and 3 of these exhibited a paradoxical pattern of dysferlin expression: severely reduced by direct immunofluorescence with overexpression on Western blots. Six patients showed amyloid deposits in muscle that extended these findings to new domains of the dysferlin gene, including the C2G domain. Correlative studies showed colocalization of amyloid with deposition of dysferlin. The present data further serve to guide clinicians facing the expensive task of molecular characterization of patients with an LGMD phenotype. Muscle Nerve 42: 14–21, 2010     

Is Levofloxacin Necessary to Prevent Postoperative Infections of Auricular Second-Intention Wounds?

BACKGROUND Surgeons may prescribe oral quinolones after auricular procedures to prevent postoperative infections, especially those caused by Pseudomonas aeruginosa .
OBJECTIVE This study compares the efficacy of levofloxacin and local wound care to local wound care alone in preventing postoperative infection of auricular second-intention wounds.
MATERIALS AND METHODS This study was a prospective, randomized trial of 84 consecutive patients (82 in the final analysis) who underwent Mohs micrographic surgery for an auricular neoplasm and had a wound left to heal by second intention. After surgery, patients were randomly assigned to receive either local wound care or local wound care with concurrent 500 mg of levofloxacin by mouth daily.
RESULTS Overall, 85.4% of patients had no complications. Complications included 12.2% of patients with inflammatory chondritis and 2.4% of patients with infection. No infections with P. aeruginosa were observed. No statistical significance was observed between the two treatment groups.
CONCLUSION Levofloxacin is not necessary to prevent postoperative infections of auricular second-intention wounds after Mohs surgery.