鲎血细胞多肽对不同肿瘤细胞生长的影响

目的：观察鲎血细胞中提取的多肽对不同肿瘤细胞生长的影响,方法：采用酸抽提法和Sephadex G-50凝胶层析从鲎血细胞中分离出含有一低分子量的肽类组分的提取物,用不同浓度的多肽分别与生长状况良好的HL－60细胞,K562细胞,HeLa细胞,SPCA－1细胞培养24h,用甲基噻唑蓝（MTT）显色法测细胞活性,结果：提取物经SDS－PAGE显示一条分子量低于14400Da蛋白区带,该多肽对不同肿瘤细胞的生长的表现不同的活性,结论：鲎血细胞多肽可以抑制肿瘤细胞的生长。     

弓形虫SAG1单基因疫苗与SAG1-ROP2复合基因疫苗的免疫效果观察

目的构建弓形虫主要表面抗原SAG1单价基因疫苗及其与棒状体蛋白ROP2的复合基因疫苗,接种BALB/c小鼠,观察疫苗的免疫保护性。方法构建重组质粒pcDNA3.1SAG1及pcDNA3.1SAG1ROP2。将两核酸疫苗分别免疫小鼠,ELISA法检测血清IgG抗体、IFNγ、IL4;流式细胞仪测定T细胞亚群;弓形虫速殖子腹腔攻击感染观察小鼠生存时间。结果获得pcDNA3.1SAG1、pcDNA3.1SAG1ROP2重组质粒;pcDNA3.1SAG1ROP2组小鼠IgG抗体(P<0.05)、IFNγ(P<0.01)及CD8+细胞比例(P<0.05)均高于pcDNA3.1SAG1组;实验组组均未测到IL4;复合基因组感染弓形虫后生存时间较单基因组延长(P<0.01)。结论弓形虫不同生活阶段的抗原基因复合疫苗较单基因疫苗具有更好的免疫保护性。     

Spinocerebellar ataxia type-1 and spinobulbar muscular atrophy gene products interact with glyceraldehyde-3-phosphate dehydrogenase

Spinocerebellar ataxia type1 (SCA1) is one of several neurodegenerative disorders caused by expansions of translated CAG trinucleotide repeats which code for polyglutamine in the respective proteins. Most hypotheses about the molecular defect in these disorders suggest a gain of function, which may involve interactions with other proteins via the expanded polyglutamine tract. In this study we used ataxin-1, the SCA1 gene product, as a bait in the yeast two-hybrid system and identified the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase as an ataxin-1 interacting protein. In addition, the yeast two hybrid data demonstrate that wild type and mutant ataxin-1 form homo and heterodimers. Physical interaction between GAPDH and ataxin-1 was also demonstrated in vitro. To investigate if GAPDH might interact with other glutamine repeat-containing proteins involved in neurodegenerative disorders, we tested its binding to the androgen receptor which is mutated in spinobulbar muscular atrophy. The androgen receptor interacts with GAPDH both in the yeast two-hybrid system and in vitro. The binding of both ataxin-1 and the androgen receptor to GAPDH does not vary with the length of the polyglutamine tract. While provocative, these findings do not address the selective neuronal loss in each of these disorders in light of the wide expression patterns of GAPDH and the respective polyglutamine containing proteins. Nonetheless, such interactions may increase the susceptibility of specific neurons to a variety of insults and initiate degeneration.      

输液管内发生化学配伍禁忌反应的调查报告

1　临床资料1.1　病例报告　患者 ,男 ,2 4岁 .因患感冒来我院内二科门诊治疗 .医师处方用 :穿琥宁注射液 2 0 m L+5 0 g· L- 1葡萄糖注射液 2 5 0 m L ,静脉滴注 ,1次 / d;硫酸妥布霉素注射液 16万 U+9g· L- 1氯化钠注射液 2 5 0 m L,静脉滴注 ,1次 / d.从处方上看 ,上述用药方案是可行的 .但在护理操作过程中 ,却出现了不同液体瓶里的两种药液在输液管内相遇时 ,发生了化学反应 ,生成了白色絮状沉淀[1 ] .幸好护理人员及时发现 ,中止输液 ,避免了一场事故的发生 .1.2　调查报告　我们对本院门诊和内二科病房作了一次调查 .1999— 0 6…     

Is Levofloxacin Necessary to Prevent Postoperative Infections of Auricular Second-Intention Wounds?

BACKGROUND Surgeons may prescribe oral quinolones after auricular procedures to prevent postoperative infections, especially those caused by Pseudomonas aeruginosa .
OBJECTIVE This study compares the efficacy of levofloxacin and local wound care to local wound care alone in preventing postoperative infection of auricular second-intention wounds.
MATERIALS AND METHODS This study was a prospective, randomized trial of 84 consecutive patients (82 in the final analysis) who underwent Mohs micrographic surgery for an auricular neoplasm and had a wound left to heal by second intention. After surgery, patients were randomly assigned to receive either local wound care or local wound care with concurrent 500 mg of levofloxacin by mouth daily.
RESULTS Overall, 85.4% of patients had no complications. Complications included 12.2% of patients with inflammatory chondritis and 2.4% of patients with infection. No infections with P. aeruginosa were observed. No statistical significance was observed between the two treatment groups.
CONCLUSION Levofloxacin is not necessary to prevent postoperative infections of auricular second-intention wounds after Mohs surgery.     

Mohs Micrographic Excision of Melanoma Using Immunostains

BACKGROUND: Mohs excision of melanoma remains controversial, in part because of concerns regarding evaluation of frozen section margins. Several immunohistochemical stains are available for melanoma that can be used on frozen sections. OBJECTIVE: To review our experience with Mohs micrographic excision of melanoma using immunostains. METHODS: Sixty-eight patients were treated, including 46 with melanoma in situ and 22 with invasive melanoma, 62 of which were on the head or neck. HMB-45, MEL-5, Melan-A (A-103), and S-100 stains were employed. RESULTS: Sixty-seven of 68 tumors were excised to clear margins, requiring an average of 2.0 layers. Immunostains greatly enhanced detection of melanoma on frozen sections. The average margin required for clearance of in situ melanoma was 8.3 mm and of invasive melanoma was 11.1 mm. Only 23 of 46 (50%) in situ melanomas were clear with < or =6 mm margins; 15 mm margins were required to clear 96% of the tumors. Eleven of 22 (50%) invasive melanomas were clear with < or =6 mm margins; 26 mm margins were required to clear 95% of the tumors. Melan-A (A-103) was the most consistently crisp and easily interpreted immunostain. CONCLUSIONS: Mohs excision of melanoma using immunostains can be useful, especially for tumors on the head and neck. For routine excision, margins wider than those currently recommended may be required to ensure tumor clearance. We recommend that (1) biopsies be stained preoperatively for Melan-A and/or HMB-45, (2) a debulking layer be obtained for permanent sections prior to Mohs layers, and positive and negative control specimens from the tumor and distant skin should be employed for comparison of staining patterns. Large-scale prospective studies of in situ and invasive melanoma on the head and neck are necessary.