Time-course of S-cone system adaptation to simple and complex fields

We examine the temporal nature of adaptation at different stages of the S-cone color system. All lights were restricted to the S-cone-only (a constant L and M) cardinal axis in color space passing through mid-white (W). The observer initially adapted to a steady uniform field with a chromaticity on the -S end of the axis or on the +S end of the axis or a complex field composed of chromaticy -S and +S (+/-S adaptation). The observer then readapted to a steady uniform field of chromaticity W for a variable length of time (i.e., 0, 0.1, 0.25, 0.5, 1.0, or 2.0 s). A probe-flash technique was used to measure S-cone discrimination at various points along the S-cone-only cardinal axis. This allowed estimation of the response of the S-cone system over an extended response range. Following exposure to the -S and +S uniform fields, sensitivity was maximal at or near the chromaticity of the initial adaptation field and decreased linearly away from the adapting point. The shift from +S to W occurred more rapidly than the shift from -S to W; both of these shifts can be described by a multiplicative scaling of the S-cone signal. Following +/-S adaptation the threshold curve initially had a shape similar to that measured following -S adaptation, but returned rapidly to the W adaptation state. The shift following +/-S adaptation cannot be described by the multiplicative model, but can be explained by a change in the shape of the non-linearity. The results suggest the existence of fast post-receptoral processes.     

Interactions between color and luminance in the perception of orientation

At the early stages of visual processing in humans and other primates, chromatic signals are carried to primary visual cortex (V1) via two chromatic channels and a third achromatic (luminance) channel. The sensitivities of the channels define the three cardinal axes of color space. A long-standing though controversial hypothesis is that the cortical pathways for color and form perception maintain this early segregation with the luminance channel dominating form perception and the chromatic channels driving color perception. Here we show that a simple interaction between orientation channels (the tilt illusion) is influenced by both chromatic and luminance mechanisms. We measured the effect of oriented surround gratings upon the perceived orientation of a test grating as a function of the axes of color space along which the gratings were modulated. We found that the effect of a surround stimulus on the perceived orientation of the test is largest when both are modulated along the same axis of color space, regardless of whether that is a cardinal axis. These results show that color and orientation are intimately coupled in visual processing. Further, they suggest that the cardinal chromatic axes have no special status at the level(s) of visual cortex at which the tilt illusion is mediated.     

Semen quality and reproductive hormones among welders —A preliminary study

Objectives Welders are exposed to intense heat, toxic metals and their oxides and toxic gases during welding, and some of these substances reported to have adverse effects on reproductive organs. Thus, the present study was planned to investigate the sperm quality as well as reproductive hormones among welders exposed to a complex mixture of pollutants. Methods Seventeen welders aged 43.8±12.0 (mean±SD) years with welding exposure of 1 to 10 years or more participated in this study. They were performing are and gas welding in a workshop. Blood (5–6 ml) and semen samples by masturbation were collected from them. Serum was separated and analyzed for reproductive hormones by RIA techniques. Semen was processed for microscopic examination. Results The results of this study indicated that sperm concentration was in the normal range (≥20 million/ml) in all seventeen welders, however, motility, morphology as well as Hypo Osmotic Swelling test (HOS) demonstrated impairment in few welders, indicating deterioration in sperm quality compared to the reported normal reference value. Compared with the normal value of hormones provided in diagnostic kits brochure, follicle stimulating hormone (FSH), leutinizing hormone (LH) and testosterone levels were in the normal range, except in two subjects who showed higher FSH and LH levels and one who showed a lower value of testosterone. Conclusions This study suggests that welding might have had some adverse effects on sperm motility, morphology and physiologic function even though sperm concentration was in the normal range. Hormonal changes did not show any definite pattern, indicating a need for further study.     

Ortho-substituted polychlorinated biphenyl (PCB) congeners (95 or 101) decrease pituitary response to thyrotropin releasing hormone

Polychlorinated biphenyl compounds (PCBs) are global environmental contaminants that cause disruption of the endocrine system in humans and wildlife. Recently, we reported that acute exposures to ortho-PCB congeners 95 (2,3,6-2',5') or 101 (2,4,5,-2',5') causes changes in the performance of the hypothalamo-pituitary-thyroid (HPT)-axis in developing rats through mechanism(s) not yet clear. The functionality of the HPT-axis was evaluated by using the thyrotropin releasing hormone (TRH) test following acute exposure to PCBs 95 or 101. Weanling female rats received PCBs 95 or 101 intraperitoneally (ip) at 32 mg/kg for 2 consecutive days and synthetic TRH was given 48 h after the last dose. Serum thyroxine (T4) levels decreased following exposure to both the congeners. In PCB 95-treated rats, serum thyroid stimulating hormone (TSH) levels were elevated in response to TRH, but were only 40% of the control response to TRH. No significant changes were seen in serum prolactin (PRL), hypothalamic dopamine (DA), thyroid gland morphology, or epithelial cell proliferation. It is suggested that these congeners, interfere with the HPT-axis by causing a subnormal response of the pituitary and thyroid to TRH stimulation.     

Assessment of the impact of model-based scatter correction on [18F]-FDG 3D brain PET in healthy subjects using statistical parametric mapping

It is recognized that scatter correction can supply more accurate absolute quantification, and that iterative reconstruction results in better noise properties and significantly reduces streak artefacts; however, it is not entirely clear whether they produce significant changes in [18F]-FDG distribution of reconstructed 3D brain PET images relative to not scatter corrected images and analytic reconstruction procedures. The current study assesses the effect of model-based scatter correction using the single-scatter simulation algorithm and iterative reconstruction in 3D brain PET studies, using statistical parametric mapping (SPM) analysis. The study population consisted of 14 healthy volunteers (6 males, 8 females; age 63-80 years). PET images were reconstructed using an analytic 3DRP reprojection algorithm with (SC) and without explicit scatter correction (NSC), as well as using an iterative ordered subset-expectation maximization (OSEM) algorithm. Calculated attenuation correction was performed assuming uniform attenuation (mu = 0.096 cm(-1)) for brain tissues when data are precorrected for scatter. The broad-beam attenuation coefficient (mu = 0.06 cm(-1)) determined from phantom studies was applied to NSC images. The images were coregistered and normalized using the default [15O]-H2O template supplied with SPM99 and an [18F]-FDG template. A t statistic image for the contrast condition effect was then constructed. The contrast comparing SC to NSC images suggest that regional brain metabolic activity decreases significantly in the frontal gyri, in addition to the middle temporal and postcentral gyri. On the other hand, activity increases in the cerebellum, thalamus, insula, brainstem, temporal lobe, and the frontal cortex. No significant changes were detected when comparing images reconstructed using analytic and iterative algorithms. It is concluded that, for some cerebral areas, significant differences in [18F]-FDG distribution arise when images are reconstructed with and without explicit SC. This needs to be considered when interpreting [18F]-FDG 3D brain PET images after applying SC.     

Information limitations in perception of shape from texture

Li and Zaidi (Li, A., and Zaidi, Q. (2000) Vision Research, 40, 217-242) showed that the veridical perception of the 3-dimensional (3D) shape of a corrugated surface from texture cues is entirely dependent on the visibility of critical patterns of oriented energy. These patterns are created by perspective projection of surface markings oriented along lines of maximum 3D curvature. In images missing these orientation modulations, observers confused concavities with convexities, and leftward slants with rightward slants. In this paper, it is shown that these results were a direct consequence of the physical information conveyed by different oriented components of the texture pattern. For texture patterns consisting of single gratings of arbitrary spatial frequency and orientation, equations are derived from perspective geometry that describe the local spatial frequency and orientation for any slant at any height above and below eye level. The analysis shows that only gratings oriented within a few degrees of the axis of maximum curvature exhibit distinct patterns of orientation modulations for convex, concave, and leftward and rightward slanted portions of a corrugated surface. All other gratings exhibit patterns of frequency and orientation modulations that are distinct for curvatures on the one hand and slants on the other, but that are nearly identical for curvatures of different sign, and nearly identical for slants of different direction. The perceived shape of surfaces was measured in a 5AFC paradigm (concave, convex, leftward slant, rightward slant, and flat-frontoparallel). Observers perceived all five shapes correctly only for gratings oriented within a few degrees of the axis of maximum curvature. For all other oriented gratings, observers could distinguish curvatures from slants, but could not distinguish signs of curvature or directions of slant. These results demonstrate that human observers utilize the shape information provided by texture components along both critical and non-critical orientations.     

Effect of mild whole body hyperthermia on cytotoxic action of N-methyl-N'-nitro-N-nitrosoguanidine in Swiss mice

Studies were carried out to ascertain the efficacy of mild whole body hyperthermia (WBH) as a modifier of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) cytotoxicity in mice. Adult Swiss male mice, 6-8 weeks old, weighing about 25 g were exposed to mild WBH (39 degrees C, 1 h) in a precision temperature controlled environmental chamber maintained at 50-60% relative humidity. Twenty-four hours after treatment, animals were administered with different doses of MNNG either by intraperitoneal (i.p.) injections or by feeding through drinking water and were monitored for survival. The studies revealed that the exposure of animals to mild WBH, 24 h prior to MNNG administration results in an increase in survival and recovery in mean body weight compared with those administered with MNNG only. This suggests that prior WBH treatment can effectively reduce the MNNG cytotoxicity in mice.