rhBMP-2对骨骼肌卫星细胞增殖与粘附的影响

目的：探讨人重组骨形态发生蛋白(rhBMP)一2对骨骼肌卫星细胞增殖与粘附的影响。方法：体外分离与培养骨骼肌卫星细胞，分别用0、50、100、500、1000ng/ml的rhBMP-2诱导培养基培养48h。利用MTT法测定细胞增殖能力的变化，通过荧光法测定接种后1h的粘附细胞率。结果：rhBMP-2可促进骨骼肌卫星细胞的增殖，这种作用从BMP浓度为500ng/ml即可表现出来，并随着浓度的增加而越发明显。在rhBMP-2作用下骨骼肌卫星细胞的粘附率增高，在500ng/ml的浓度时达最高，但当BMP浓度进一步增加时，细胞粘附率却不再增加。结论：rhBMP-2可促进骨骼肌卫星细胞的增殖，增强其粘附特性。     

Aetiology and outcome of acute renal failure secondary to war related trauma and infectious disease in Croatia

Summary: The aim of our study was to explore the aetiology and the outcome of acute renal failure (ARF) during the war in Croatia. of the 2132 patients admitted to our hospital between April 1990 and November 1992 due to war related trauma, 11 (0.5%) developed ARF. We believe that the development of ARF in these patients was secondary to an overwhelming septic process. Most of our patients suffered from multiple organ failure. of the 11 patients suffering from ARF due to war related trauma only four recovered (63.6% had died). We attribute the lethal outcome to the progression of the septic process. Patients who developed ARF due to infectious diseases unrelated to trauma had a different prognosis. Acute renal failure caused by the Hantan virus ran a benign course, in both its oliguric and non-oliguric form. Patients who developed ARF as a complication of leptospirosis also had a good prognosis. Although ARF is usually of a multifactorial genesis, our study aimed to emphasize the importance of disseminated septic processes as a cause of ARF.     

Medical Progress in China }， RECENT ADVANCES IN OBSTETRICS AND GYNECOLOGY IN CHINA

With the progress in perinatology, due attention has been paid to Women Health Care in the last few years in China. Since 1985 when the OB/GYN Society set up a Section for Women Health Care, several symposia have been held on women health care from early childhood to the elderly. In 1988, under the guidance of the Preventive Medical Association, maternal and child health care workers came together to organ- ize the Sociaty of Women and Children Health Care.     

日本岛津DSA血管机常见应用故障剖析

剖析了数字减影设备在介入放射治疗手术中因使用、操作不当造成DSA设备应用故障的实例，而合理使用、熟练操作是可以减少或避免该类应用故障的发生。     

低浓度二甲基亚砜冻存外周血造血干细胞的效果

目的:观察外周血造血干细胞(PBSC)用低浓度二甲基亚砜(DMSO)和羟乙基淀粉(HES)在-80℃条件下冷冻保存的效果。方法:49例次患者的PBSC在冻存后3、6个月及1年进行复苏,分别取样进行有核细胞(NC)计数、锥虫蓝拒染率测定、CD34 细胞阳性率分析和粒-巨噬细胞集落生成单位(CFU-GM)检测。结果:PB-SC在-80℃冰箱中冻存3个月和6个月的NC、CD34 细胞、CFU-GM回收率的差异均无统计学意义(均P>0.05),NC活细胞比率差异也无统计学意义(P>0.05)。PBSC冻存1年NC、CD34 细胞、CFU-GM集落回收率及活细胞比率均有显著性下降(均P<0.01)。结论:应用低浓度DMSO在-80℃冻存PBSC3个月和6个月的细胞能得到较好保存,1年后的冻存效果显著下降。     

Primordial germ cells are capable of producing cells of the hematopoietic system in vitro

The identity of the cells giving rise to the hematopoietic system in the mouse embryo are unknown. The results presented here strongly suggest that hematopoietic cells are derived from a nonhematopoietic cell population that has been previously thought to give rise to the germ cells. These cells are called primordial germ cells (PGCs) and can be recognized as large cells showing blebbing and pseudopodial extrusions on their surface. They are alkaline phosphatase (AP) positive and possess a stage-specific embryonic antigen (SSEA-1) on their surface. They represent a small pool of cells in the extraembryonic mesoderm at the base of the allantois in late day-6 embryos. Primordial germ cells from 7.5- and 8.5-day visceral yolk sac and embryo proper form AP+ and SSEA-1+ colonies within 5 days when grown on an embryonic fibroblast feeder cell layer in the presence of leukemia inhibitory factor (LIF), stem cell factor (SCF), and interleukin-3 (IL-3). Individual colonies taken from day-5 cultures can be shown to differentiate into erythroid lineage cells in secondary methyl cellulose culture and produce secondary and tertiary PGCs in the presence of LIF, SCF, and IL-3. Cells taken from the region of the allantois and primitive streak can form colonies on hydrophilic Teflon (DuPont, Wilmington, DE) foils precoated with collagen and fibronectin. The cells from these colonies were then shown to form cobblestone areas on irradiated adult bone marrow stromal layers, indicating that the most primitive in vitro hematopoietic stem cell, the cobblestone-area forming cell (CAFC), was present. PGC colonies were grown in methyl cellulose in the presence of LIF, SCF, and IL-3 for 5 days, and the colonies were removed and passaged 3 times on pretreated extracellular matrix hydrophilic Teflon foils. After each passage, the cells were assayed for their differentiation capacity and PGC content. After the last passage, the number of CAFCs was also determined. It was found that, under these conditions, the PGC population expanded more than 400- fold and also contained CAFCs. It is postulated that the PGC represents a totipotent stem cell population capable of producing a variety of different cell types including cells of the hematopoietic system.     

后腹腔镜肾输尿管全切加腹膜后肾周区域淋巴结清扫与开放手术治疗UUT-TCC疗效比较

目的:探讨后腹腔镜技术与开放手术治疗上尿路移行细胞癌(upper urinary tract transitional cell carcinoma,UUT-TCC)的效果及优势。方法:回顾性分析2009年11月~2012年12月间,我院采用后腹腔镜下肾输尿管根治性切除术加腹膜后肾周区域淋巴结清扫术治疗肾盂输尿管癌患者23例(腹腔镜组),并与同期14例行传统开放性肾输尿管切除术患者(开放术组)的手术时间、出血量、肿瘤学预后等指标进行比较。结果:腹腔镜组患者经术后病理检查,肾盂癌24例,输尿管癌10例,输尿管癌伴膀胱癌3例,其中腹腔镜组和开放组淋巴结转移各1例。同时研究发现:①腹腔镜组与开放术组比较,手术时间缩短,出血量明显减少,术后肠胃功能恢复快,术后住院时间短,差异有统计学意义(P0.05);②两种术式肿瘤学预后在远处转移方面,腹腔镜组更少,差异有统计学意义(P0.05)。结论:后腹腔镜下肾输尿管全长切除加经尿道膀胱袖状切除并腹膜后肾周区域淋巴结清扫治疗UUT-TCC具有创伤小、痛苦少、术后恢复快等优点,可能有更好的肿瘤学预后。