国产唑来膦酸治疗肿瘤高钙血症多中心Ⅱ期临床观察

Objective： To evaluate the effect and safety of clinical use of zoledronic acid in the treatment of malignant hypercalcemia. Methods： A multi-center, open phase II clinical trial was conducted in 15 cases with malignant hypercalcemia who received zoledronic acid intravenously for 15 min. The level of blood calcium and side effects were recorded regularly within 28 days after injection. Results： One case was dropped out due to bad compliance. The complete response rate （the corrected serum calcium was reduced to normal level） was 100.00% （14/14）. The medium time of complete response rate was 5.07 days. The medium maintain time was 22.30 days. Slight, or moderate fever was observed. Conclusion： Zoledronic acid can effectively reduce the malignant hypercalcemia. The use of zoledronic acid appears to be safety and convenient.     

特异性荧光偏振免疫法监测521例肾移植后环孢素A全血浓度3275次

目的通过监测肾移植后病人环孢素A(CsA)全血浓度 ,提出CsA在三联免疫抑制用药方案中的理想治疗窗。方法用特异性荧光偏振免疫法测定CsA全血浓度 ,对521例病人监测3275次 ,按术后时间及临床表现分组比较。结果肾移植后<1 ,、1～3、3～6、6～12个月、1～2和>2年的CsA全血谷浓度的理想治疗窗应分别为250～450、200～400、150～300、100～250、100～200和100～180μg/L。结论CsA全血浓度在上述范围内 ,中毒反应和排异反应明显减少     

排粪造影的临床应用

介绍排粪造影方法，报告１６０例造影结果。对照组５２例中２例（３８５％）异常；排粪障碍组１０８例，仅１８例（１６６７％）未见异常，提出了肛直角和肛上距正常测量值。报道主要异常Ｘ线表现。探讨了排粪造影检查对于肛管直肠部及盆底疾病的诊断价值     

Construction of retroviral vectors to induce a strong expression of human interferon-β gene in human hepatoma cells

ClassIinterferonisapotent1mmunemodu-latingfactorinvivo,andplaysanimportantrolelnactivatinganti-tumorcytotoxicTlymphocytesandnonuspecificeffectorcellssuchasNKcellsLlJ.ln-terferonandmanyothercytokinegeneswereintro-ducedintotumorcells,tumorinterstitialcellsandtumorinfiltratinglymphocytes['-']inordertosustainaneffectiveanti-tumorconcentrationofcy-tokinelocatedattumorsites.Itwasshownthatsomecytokinegenemodifiedtumorcellslosttheirtumorigenicityandinhibitedthegrowthofparentaltumorinanimalmodels.Pra…     

铁缺乏时淋巴细胞增殖反应改变的实验研究

本研究采用体外细胞培养，３Ｈ－Ｔｄｒ掺入，原子吸收光谱分析等技术方法，观察了低浓度Ｆｅ－ｃｉｔ时淋巴细胞内铁浓度变化及经ＣｏｎＡ、ＰＨＡ诱导的细胞增殖反应能力改变，结果显示：铁缺乏时（第２、３、４组）ＳＩ降低，与正常组（第１组）比较相差非常显著（Ｐ＜０．０１），ＳＩ与细胞内铁浓度的变化呈显著正相关（Ｐ＜０．０５，ｒ１＝０．９４６６，ｒ２＝０．９３３９），说明铁缺乏时淋巴细胞增殖反应能力受抑。     

金丹肝泰1号对大鼠急性肝损伤防治作用的实验研究

用D-氨基半乳糖（D-GaIN）制备大鼠急性肝损伤模型。方法：检测血清丙氨酸氨基转氨酶（ALT）和血清天门冬氨酸氨基转氨酶（AST）的活性，观察光镜下肝组织的病理学改变，探讨对急性肝损伤的保护作用。结果：口服浸膏可显降低急性肝损伤大鼠血清ALT和AST水平，减轻肝细胞的变性坏死，其作用强度与肝炎宁相当，但弱于联苯双酯。     

Diagnosis and treatment of thoracic outlet syndrome

Patients who develop symptoms of thoracic outlet syndrome (TOS) have a predisposing anatomic abnormality. In most patients with TOS, the symptoms are caused by entrapment of the brachial plexus and they do not arise from compression of the subclavian artery, as was previously thought. The tests advocated for diagnosing this common syndrome (i.e., evaluating the positional compression of the artery when the arms are raised, the neck is turned, or the shoulders are braced) cannot accurately diagnose this syndrome. There are two reasons for this. The symptoms of TOS are not related to the compression of the artery in the outlet in 98% of patients, and 75% of normal individuals without symptoms show diminished radial pulse on various provocation tests. We employed four timed provocation tests (minute tests) to diagnose TOS: the timed Morley test, timed Wright test, timed Eden test, and elevated arm stress exercise, all of which are very sensitive. In normal individuals without symptoms, 20% experience transitional symptoms such as slight pain and tiredness, on these tests indicating a subclinical state. TOS is treated by keeping the thoracic outlet wide, this being done either conservatively or surgically. In 1993 and 1994, we conservatively treated 418 of 422 patients with TOS by means of active exercise, a brace, and by block therapy. These measures did not reduce the symptoms in 23 of these patients, so surgical treatment was indicated. In the remaining 4 of the 422 patients, conservative treatment was not indicated and surgery was performed directly. All the patients showed significant clinical improvement of varying degree. Presented at the 69th Annual Meeting of the Japanese Orthopaedic Association, Tokyo, April 12, 1996     

乙基叔丁醚对动物局部和全身的毒性研究

为了主人乙基叔丁醚（ＥＴＢＥ）的毒性作用，探讨它的灌注溶解治疗胆固醇结石的可能性。我们做了该药物的动物毒性实验，对比ＥＴＢＥ和ＭＴＢＥ（甲基叔丁醚）对动物的局部组织和全身毒性作用。局部毒性实验是对大白兔胆囊进行灌注后，病理观察组织变化。全身毒性实验是通过对小鼠腹腔注射药物的半致死量作对比。结果发现，ＥＴＢＥ对兔的胆囊、胆部属这和十二指肠的损害较ＭＴＢＥ组动物轻。ＥＴＢＥ摇篮腔注射半致死量ＬＤ５０）     

Endocrinology: High incidence of embryo transfer cancellations in patients with polycystic ovarian syndrome

This study was aimed at assessing the outcome of in-vitro fertilization(IVF) and embryo transfer in patients with polycystic ovariansyndrome (PCOS). The results of IVF and embryo transfer in PCOSpatients (PCOS group, 78 cycles of 26 patients) were comparedwith those of a control group (423 cycles in 202 patients withoutmale factor; age and ovarian stimulation protocol were matched).Although the pregnancy rate per transfer was not different inthe two groups of patients (25 versus 34%, PCOS versus controlgroup), the PCOS group had a significantly lower pregnancy rateper follicle aspiration (19 versus 31%, P < 0.05). A notableresult was a significantly higher incidence of embryo transfercancellations in the PCOS group (22 versus 8%, P < 0.01),which resulted from unpredictable failure of either oocyte recoveryor fertilization. The incidence of unexplained complete failureof fertilization was significantly higher in the PCOS group(18 versus 5%, P < 0.01). These results may reflect a reducedquality of the oocytes in the PCOS group, and there was a subgroupof PCOS patients who repeatedly produced poor results of treatment.Although the ovarian stimulation regimen best suited to PCOSpatients remains to be determined, special care should be takenduring ovarian stimulation, especially when the PCOS patientshad experienced unexplained failure of oocyte recovery or fertilizationin the previous treatment cycle(s).