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排序方式: 共有7387条查询结果,搜索用时 781 毫秒
131.
Thiago Martins Lara Ludhmila Abrahao Hajjar Juliano Pinheiro de Almeida Julia Tizue Fukushima Carmem Silvia Valente Barbas Adriano Rogerio Baldacin Rodrigues Emilia Nozawa Maria Ignes Zanetti Feltrim Elisangela Almeida Vera Coimbra Eduardo Osawa Rafael de Moraes Ianotti Alcino Costa Leme Fabio Biscegli Jatene Jose Otavio Costa Auler-Jr. Filomena Regina Barbosa Gomes Galas 《Clinics (S?o Paulo, Brazil)》2013,68(1):33-38
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134.
Makio Kusaoi Ken Yamaji Go Murayama Misa Yasui Risa Yamada Ruka Hishinuma Takuya Nemoto Katsura Hohtatsu Michiaki Kageyama Toshio Kawamoto Kaoru Sugimoto Fumio Sekiya Takayuki Kon Michihiro Ogasawara Kazuo Kempe Hiroshi Tsuda Yoshinari Takasaki 《Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy》2012,16(5):456-466
Leukocytapheresis (LCAP) is a safe, unique therapy pertaining to intractable rheumatoid arthritis (RA) even in cases of drug allergy or infectious states. To investigate how to represent LCAP efficacy, we have conducted gene expression analyses from the peripheral blood of RA patients treated with non‐woven polyethylene terephthalate filters. Peripheral blood samples were collected immediately before and after treatment from eight RA patients who received LCAP. Among these patients, all of them achieved 20% improvement in the core set of the American College of Rheumatology (ACR20), and thus, they were confirmed as LCAP responders. Gene expression analysis was done with a high‐resolution DNA microarray. The results of each of the two groups' gene expression values (immediately before and after LCAP) were calculated using Welch's t‐test. Calculations were performed with a statistical software R.basic package: if the P‐value was less than 0.05, this was seen as a significant change. In a comparison of 25 370 gene expressions, the number of genes showing a P‐value < 0.05 in the upregulating group was 2110, and in the downregulating group it was 1864. The results of pathway analysis using the MetaCore program indicate that gene groups work for cytoskeletal remodeling are upregulated, and genes related to immune responses, such as antigens presenting via major histocompatibility complex class I and II, are downregulated just after LCAP. These findings may relate to LCAP efficacy for RA patients, but this needs further investigation. 相似文献
135.
Nhu Ngoc Quynh Vo Ery Odette Fukushima Toshiya Muranaka 《Journal of natural medicines》2017,71(1):50-58
We evaluated the hemolytic activity of 41 commercially available triterpenoid saponins and sapogenins derived from three types of structural skeletons. Structure–activity relationships were established by comparing the structural characteristics of both the aglycone and sugar moieties among the tested compounds. The majority of oleanane-type sapogenins had stronger hemolytic effects than those of the ursane and dammarane types. The presence of polar regions on sapogenins, such as a carboxyl (COOH) at position 28, an α-hydroxyl (α-OH) at position 16, and/or a β-hydroxyl (β-OH) at position 2, significantly enhanced hemolysis. Meanwhile, the introduction of an α-OH at position 2 or a methyl hydroxyl (CH2OH) at positions 23 or 24 was closely associated with reduced activity. Our findings suggest that not only the complexity of sugar moieties but also the types and stereochemical configurations of functional groups at different positions, as well as the skeleton types, are important structural features affecting hemolytic potential. Our results provide a baseline in terms of the toxicity of saponins and sapogenins to erythrocytes, which holds promise for drug development. 相似文献
136.
Rie Fukui Eriko Tanabe Misaho Kitayoshi Kyohei Yoshikawa Nobuyuki Fukushima Toshifumi Tsujiuchi 《Tumour biology》2012,33(6):1899-1905
Lysophosphatidic acid (LPA) mediates a wide range of biological responses with G protein-coupled transmembrane receptors (LPA receptors). So far, at least six types of LPA receptors (LPA receptor-1 (LPA1) to LPA6) have been identified. Recently, it has been reported that LPA3 indicates opposite effects on cellular functions of cancer cells. In the present study, to assess a biological role of LPA3 on cell migration ability of colon cancer cells, we generated LPA receptor-3 (LPAR3) knockdown (HCT-sh3-3) cells from HCT116 and measured cell motile and invasion activities. In motility assay with a cell culture insert, HCT-sh3-3 cells showed significantly high cell motile activity, compared with control cells. For invasion assay, the filter was coated with Matrigel. The invasive activity of HCT-sh3-3 cells was significantly higher than that of control cells. Furthermore, we also examined the effects of LPAR3 knockdown on the interaction between colon cancer cells and endothelial F-2 cells. When F-2 cells were cultured with serum-free DMEM containing a supernatant from HCT-sh3-3 cells, the cell growth rate and migration activity of F-2 cells were significantly stimulated, associating with the elevated expressions of vascular endothelial growth factor (VEGF)-A and VEGF-C genes in HCT-sh3-3 cells. These results suggest that LPA3 may act as a negative regulator on cell motile and invasive abilities of colon cancer HCT116 cells. 相似文献
137.
Tomoyoshi Shibuya Keiichi Haga Michio Saeki Mayuko Haraikawa Hitoshi Tsuchihashi Koki Okahara Osamu Nomura Hirofumi Fukushima Takashi Murakami Dai Ishikawa Shigaku Ikeda Akihito Nagahara 《Journal of clinical apheresis》2020,35(5):488-492
Pyoderma gangrenosum (PG) is an extra-intestinal skin lesion in inflammatory bowel disease (IBD) as is erythema nodosum. Vedolizumab (VED) is a monoclonal antibody that targets α4β7 integrin and has an intestinal selective mechanism. Despite good therapeutic effects on colitis, the effect on extra-intestinal manifestations (EIMs) remains unclear. Here we report a case of ulcerative colitis complicated by PG during treatment with VED, which was successfully treated with prednisolone in combination with adsorptive granulocyte and monocyte apheresis (GMA). The patient was a 50-year-old woman with a past medical history of extensive ulcerative colitis managed by golimumab (GLM). She developed flare symptoms due to loss of response to GLM, and treatment was switched to VED. Her gastrointestinal symptoms were improved with VED treatment with less frequent bowel movements. However, infiltrative erythema with pain appeared on the right lower leg and right knee, and expanded and gradually ulcerated. Her skin lesions were treated with corticosteroid, but showed poor improvement. Therefore, granulocyte and monocyte apheresis (GMA) treatment was administered in combination with prednisolone. After 3 months, the ulcer gradually improved, and at the time of this writing, the eruptions were nearly replaced by epithelial tissue. This case study showed that patients with UC and EIMS may respond well to combination therapy of VED and GMA. GMA has a very favorable safety profile. On the other hand, the causal connection between VED and PG is still unclear. We believe that a combination therapy involving VED and GMA in IBD patients with EIMs warrants consideration. 相似文献
138.
Mihoko Hatano Yoshihiro Matsumoto Jun-ichi Fukushi Tomoya Matsunobu Makoto Endo Seiji Okada Kunio Iura Satoshi Kamura Toshifumi Fujiwara Keiichiro Iida Yuko Fujiwara Akira Nabeshima Nobuhiko Yokoyama Suguru Fukushima Yoshinao Oda Yukihide Iwamoto 《Clinical & experimental metastasis》2015,32(6):579-591
139.
Kazumi Urata Ikko Kajihara Tselmeg Mijiddorj Myangat Yukino Tasaki Saki Otsuka‐Maeda Soichiro Sawamura Saori Kanazawa‐Yamada Ryoko Sakamoto Katsunari Makino Jun Aoi Toshikatsu Igata Takamitsu Makino Shinichi Masuguchi Satoshi Fukushima Masatoshi Jinnin Hironobu Ihn 《The Journal of dermatology》2019,46(5):444-448
Cyclin‐dependent kinase 4 and 6 (CDK4/6) plays an important role in cell cycle progression, and the CDK4/6–cyclin D1 complex controls the cell cycle transition from G1 phase to S phase. CDK4 is enhanced in several types of cancers and CDK4/6 inhibitors attenuate the proliferation of several types of cancer in vitro/in vivo. The purpose of our study was to investigate the expression pattern of CDK4 and evaluate its clinical importance in extramammary Paget's disease (EMPD). Almost all EMPD tissues were positive for CDK4, and metastatic lesions had a similar immunostaining intensity to primary lesions. In addition, CDK4 protein levels were positively correlated with those of cyclin D1 protein. Taken together, CDK4 may assume a crucial role in EMPD progression. 相似文献
140.
Byung-Ho Yoon Lynne C. Jones Chung-Hwan Chen Edward Y. Cheng Quanjun Cui Wolf Drescher Wakaba Fukushima Valerie Gangji Stuart B. Goodman Yong-Chan Ha Philippe Hernigou Marc Hungerford Richard Iorio Woo-Lam Jo Vikas Khanduja Harry Kim Shin-Yoon Kim Tae-Young Kim Kyung-Hoi Koo 《The Journal of arthroplasty》2019,34(1):169-174.e1