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排序方式: 共有785条查询结果,搜索用时 15 毫秒
21.
Narushima M Uchigashima M Hashimoto K Watanabe M Kano M 《The European journal of neuroscience》2006,24(8):2246-2252
Endogenous cannabinoids (endocannabinoids) act as retrograde inhibitory messengers in various regions of the brain. We have recently reported that endocannabinoids mediate short-term retrograde suppression of excitatory synaptic transmission from the neocortex to medium spiny (MS) neurons, the major projection neurons from the striatum. However, it remains unclear whether endocannabinoids modulate inhibitory transmission in the striatum. Here we show that depolarization of MS neurons induces transient suppression of inhibition that is mediated by retrograde endocannabinoid signalling. By paired recording from a fast-spiking (FS) interneuron and an MS neuron, we demonstrated that FS-MS inhibitory synapses undergo endocannabinoid-mediated retrograde suppression. We verified that GABAergic inhibitory terminals immunopositive for parvalbumin (PV), a marker for FS interneurons, expressed CB1 receptors. These PV-CB1 double-positive terminals surrounded dopamine D1 receptor-positive and D2 receptor-positive MS neurons; these constitute direct and indirect pathways, respectively. These results suggest that endocannabinoid-mediated retrograde suppression of inhibition influences information flow along both direct and indirect pathways, depending on the activity of MS neurons. 相似文献
22.
The present investigation focused on the transepithelial flux of liquiritigenin (LG), davidigenin (DG), liquiritin (LQ), and liquiritin apioside (LA) using the human colonic cell line Caco-2 as a model of human intestinal absorption. Apparent permeability coefficients (Papp) for the apical to basolateral flux of LG and DG were (16.0 +/- 0.727) x 10(-6) cm/s and (18.2 +/- 1.67) x 10(-6) cm/s, respectively. These Papp were higher than that of the transcellular transport marker propranolol (13.5 +/- 0.34) x 10(-6) cm/s (P < 0.01). Papp for the apical to basolateral flux of LQ and LA were (0.26 +/- 0.12) x 10(-6) cm/s and (0.075 +/- 0.005) x 10(-6) cm/s, respectively. These Papp were lower than that of the paracellular transport marker mannitol (0.64 +/- 0.04) x 10(-6) cm/s (LG, P < 0.01; LA, P < 0.001). These data suggested excellent absorption of LG and DG through the human intestinal epithelial cell line. On the contrary, poor absorption of LQ and LA was expected due to the little transepithelial flux of these compounds in the human colonic cell line Caco-2. 相似文献
23.
Koyanagi M Imanishi K Arimura Y Kato H Yagi J Uchiyama T 《International immunology》2004,16(2):315-326
To determine the levels of maturation and differentiation ofmurine CD4 single-positive (SP) T cells, we compared the secondaryresponses of staphylococcal enterotoxin A (SEA)-induced neonatalthymic, adult thymic and adult splenic CD4 SP T cell blastsprepared from whole or heat-stable antigenlow CD4 SP T cells.Proliferative responses upon re-stimulation with SEA were strongin adult splenic CD4 SP T cell blasts, but quite weak in neonatalthymic and adult thymic CD4 SP T cell blasts. SEA-induced IL-2production was weaker in neonatal thymic blasts than in theadult splenic CD4 SP T cell blasts. In contrast, SEA-inducedIL-4 production was high in neonatal thymic CD4 SP T cell blasts,and low in adult splenic and thymic CD4 SP T cell blasts. Expressionof GATA-3, that directs production of IL-4 in T cells, examinedat protein and mRNA levels, was higher in neonatal thymic cellsthan in adult thymic and splenic cells. These results suggestthat neonatal and adult thymic CD4 SP T cells in the final stageof maturation are relatively immature compared with adult splenicCD4 SP T cells. The cytokine production profile of neonatalthymic CD4 SP T cells suggests that they are inclined towardsa Th2 response. 相似文献
24.
Detection of novel events is crucial for adapting to changing environments. The prefrontal cortex has been thought to be one of the areas involved in orienting attention to novel events. Here, we examined the effects of two components of novelty: context novelty, which purely happens when a familiar event occurs in an unpredicted situation or time and feature novelty, which happens by itself when an unfamiliar stimulus appears against the expectation of familiar ones. We trained monkeys on a task that included both novelty components and recorded the activity of neurons in the lateral and medial divisions of the prefrontal cortex. The responses of a substantial number of cells in both the lateral and medial divisions were enhanced when a familiar visual stimulus was presented in an unpredicted context. By contrast, enhancement of responses by the unfamiliarity of visual stimuli was observed mainly in cells in the lateral prefrontal cortex. These results suggest that the lateral and medial divisions of the prefrontal cortex are differentially involved in the control of attention triggered by novel sensory events. 相似文献
25.
Honda K Zheng N Murakoshi H Hashimoto M Sakai K Borghan MA Chikata T Koyanagi M Tamura Y Gatanaga H Oka S Takiguchi M 《European journal of immunology》2011,41(1):97-106
HIV-1 mutants escaping from HLA-A- or HLA-B-restricted CTL have been well studied, but those from HLA-C-restricted CTL have not. Therefore we investigated the ability of HLA-C-restricted CTL to select HIV-1 escape mutants. In the present study, we identified two novel HLA-Cw(*) 1202-restricted Pol-specific CTL epitopes (Pol328-9 and Pol463-10). CTL specific for these epitopes were detected in 25-40% of chronically HIV-1-infected HLA-Cw(*) 1202(+) individuals and had strong abilities to kill HIV-1-infected cells and to suppress HIV-1 replication in vitro, suggesting that these CTL may have the ability to effectively control HIV-1 in some HLA-Cw(*) 1202(+) individuals. Sequence analysis of these epitopes showed that a V-to-A substitution at the 9th position (V9A) of Pol 463-10 was significantly associated with the HLA-Cw(*) 1202 allele and that the V9A mutant was slowly selected in the HLA-Cw(*) 1202(+) individuals. Pol 463-10-specific CTL failed both to kill the V9A virus-infected cells and to suppress replication of the V9A mutant. These results indicate that the V9A mutation was selected as an escape mutant by the Pol463-10-specific CTL. The present study strongly suggests that some HLA-C-restricted CTL have a strong ability to suppress HIV-1 replication so that they can select HIV escape mutants as in the case of HLA-A-restricted or HLA-B-restricted CTL. 相似文献
26.
Iwasaki Y Shibata N Ninomiya M Kurita K Nakabayash N Ishihara K 《Journal of biomaterials science. Polymer edition》2002,13(3):323-335
Heparinization is believed to be one of the methods to suppress thrombus formation on blood-contacting surfaces. However, this study hypothesizes that heparinization alone might not be sufficient to provide a blood-compatible surface; that is, a surface property that resists biofouling is necessary to obtain an effective heparin-modified surface. 2-Methacryloyloxyethyl phosphorylcholine (MPC) polymers with 2-aminoethyl methacrylate (AEMA) were synthesized to immobilize heparin through ionic bonding. The primary amino groups of AEMA were considered to be the polymer surface because the zeta-potential of the surface was positive when the mole fraction of the AEMA units was above 0.2. The antithrombogenic character of the polymer surface modified with heparin was evaluated by both Lee-White and microsphere column methods. The coagulation period of human whole blood in the absence of anticoagulant in glass tubing coated with the MPC polymer was longer than that in the original glass tube. Cell adhesion was completely inhibited on the MPC polymer surface after contact with human whole blood without anticoagulant. However, many adherent blood cells were observed on poly(2-ethylhexyl methacrylate-co-AEMA) (no MPC unit) even after heparinization. These results strongly indicate that the MPC polymer is a useful substrate where the heparin works well and that the heparin-immobilized MPC polymer has superior blood compatibility to the simple MPC polymer. 相似文献
27.
Konno T Ito T Takai M Ishihara K 《Journal of biomaterials science. Polymer edition》2006,17(12):1347-1357
We prepared phospholipid polymer nanoparticles immobilized with luciferase, and the nanoparticles were applied as photochemical sensing nanoparticles. An amphiphilic water-soluble polymer having a phosphorylcholine group was used as an emulsifier and a surface modifier to prepare the nanoparticles. The polymer was composed of three kinds of monomer units, that is, 2-methacryloyloxyethyl phosphorylcholine (MPC) as a hydrophilic and bioinert unit, n-butyl methacrylate as a hydrophobic unit and p-nitrophenyl ester having methacrylate as an enzyme-immobilizing unit. The p-nitrophenyl ester groups to immobilize the proteins were located on the surface of the nanoparticles. Luciferase was immobilized by the reaction between the p-nitrophenyl ester groups and the amino group. The enzymatic reaction on the nanoparticles was followed using a microdialysis system with an optical fiber having a 800 microm diameter in the probe. The nanoparticles conjugated with luciferase reacted with ATP, luciferin and oxygen. It is concluded that the nanoparticles are a promising tool for a photochemical sensing microdiagnostic system. 相似文献
28.
Anudep Rungsipipat Madoka Oda Noavarat Kumpoosiri Supradit Wangnaitham Rosama Poosoonthontham Wuthichai Komkaew Fahnan Suksawat Yamaguchi Ryoji 《Comparative clinical pathology》2009,18(1):13-22
The aims of this study are to investigate the hematology, blood chemistry, pathological study, including macroscopic and microscopic
lesions, of experimentally induced canine monocytic erhlichiosis in Thailand and to demonstrate the distribution of Ehrlichia canis in target organs by nested polymerase chain reaction (PCR). Five experimental healthy dogs were inoculated with 5 ml of whole
blood (estimated number of E. canis morulae 15 × 10–5% per monocytes) from the splenectomized dog via the saphenous vein. Two healthy dogs served as a negative control. Hematology
revealed nonregenerative normocytic normochromic anemia, thrombocytopenia and mild leukopenia. Blood chemistry revealed an
increase in aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (AP), hypoproteinemia,
hypoalbuminemia, and hyperglobulinemia by day 66 post-inoculation. Pathology revealed anemia, ascites, jaundice, interstitial
pneumonia, splenomegaly, generalized lymphadenopathy, and severe fatty liver. The detection of E. canis was performed using tissue embedded in paraffin wax by nested PCR showing positive in all target organs. This study concluded
that acute induced experimental canine monocytic ehrlichiosis can cause significant clinical and pathological lesions. 相似文献
29.
Wada T Maeda H Kawagoe S Fujiyama T Matsuzaki M Nashiki K 《Gan to kagaku ryoho. Cancer & chemotherapy》2002,29(Z3):429-431
We present here the EMInet (Electronic Medical Information network), a closed information network established by the City government and the Medical Association in Matsudo City, Chiba, Japan. In this network, medical information is shared by multiple certified medical facilities under the informed consent of the patients. The medical record is written only by the physician in charge of the patient and reserved in the server computer. The medical record cannot be rewritten or modified by anyone except the physician in charge of the patient. Other medical professionals can only have access to the record. In the emergency room of certified hospitals, a physician can open the patient's record by using the IC card carried by the patient. 相似文献
30.
The object of this study is to investigate the relationship between a typical product of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine (8OHdG), and mutagenesis in V79 cells through a molecular analysis of hypoxanthine-guanine phosphoribosyltransferase ( hprt ) gene mutants. We performed a direct sequencing analysis of the cDNA of mutants obtained after treatment with N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-l,4,5,8-naphthalenetetracarboxylic-diimide (NP-III) or riboflavin, each of which induces the formation of 8OHdG in cellular DNA upon UVA irradiation. The frequency of mutation after both treatments was no more than 2 to 5 times the control value. A considerable number of the mutants could not be amplified by RT-PCR, and this was also the case for the control mutants. Among the mutants analyzed, deletions and a TA→Ã transversion occurred predominantly. The reasons for the weak association of induction of 8OHdG with frequency of mutation and the possible mechanism of oxidative-stress-derived mutagenesis are discussed. 相似文献