抗心肌缺血药物的作用靶点

缺血性心脏病严重危害人类健康,一直是医学界研究的热点.本文主要介绍与心肌缺血相关的几个药物的作用靶点:离子通道相关靶点、内皮素受体、转录调节因子、L-精氨酸一氧化氮途径以及肾素-血管紧张素系统等.     

p14ARF、ARF-BP1及c-myc mRNA在肝细胞癌中的表达及临床意义

目的:探讨肝细胞癌组织中p14ARF、ARF-BP1 及c-myc的mRNA表达及其与临床参数关系.方法:采用半定量逆转录.聚合酶链式反应(RT-PCR)技术,对52例HCC组织及其中45例相应癌旁组织p14ARF、ARF-BP1及c-myc的mRNA表达丰度进行检测并分析他们之间的关系及其与临床参数的关系.结果:在52例HCC组织中p14ARF、ARF-BP1c-myc的mRNA各表达77.0%、77.0%和75.0%,与45例相应癌旁组织(11.1%、20.O%、53.3%)比较,呈高表达.p14ARF、ARF-BP1 mRNA的表达与肿瘤大小有关(t=2.169,2.087,均P＜0.05);而c-myc mRNA表达与肿瘤大小无关.p14ARF、ARF-BP1及c-myc的mRNA表达在性别、年龄、AFP、临床病理分级、浸润转移、HBV、包膜等无统计学差异.p14ARF与ARF-BP1、p14ARF与c-myc及ARF-BP1与c-myc的mRNA表达两两成正相关(r=0.565,0.436,0.584,均P＜0.01).结论:p14ARF、ARF-BP1、c-myc过度表达可能是HCC发生的早期指标;ARF-BP1过度表达可能是影响HCC发生、发展的关键,干预ARF-BP1可能是治疗HCC的新途径.     

HBV感染者血清中HBVcccDNA、HBeAg及HBV DNA的关系

探讨HBV感染者血清HBVcccDNA与血清HBV DNA及HBeAg的关系。分别以PER分子信标技术和ELISA方法对非HBV相关肝炎、HBV健康携带者、急性乙型肝炎（AHB）、慢性乙型肝炎（CHB）、乙肝肝硬化、乙肝患者血清中HBVcccDNA HBV DNA含量及HBeAg进行了检测。HBVcccDNA仅见于HBV DNA阳性血清中；HBeAg阳性组的HBVcccDNA阳性率显著高于HBeAg阴性组（P〈0．05）；145例HBV DNA阳性患者中，HBVcccDNA阳性组HBVD—NA水平显著高于HBVcccDNA阴性组（P〈0．01）。血清HBVcccDNA可能是乙肝病毒在患者体内大量复制的血清标志。     

IL-2诱导类风湿关节炎与骨关节炎T淋巴细胞STAT3 STAT5酪氨酸磷酸化状态的比较

目的观察白细胞介素(IL)-2作用于类风湿关节炎(RA)与骨关节炎(OA)外周血T淋巴细胞前后,T淋巴细胞中STAT3及STAT5的酪氨酸磷酸化活化状态,并进行比较.方法从RA与OA患者的外周血中分离培养单个核细胞,继而纯化得到T淋巴细胞,静息后,用重组人IL-2刺激,在各时相裂解细胞,收获提取蛋白,进行Western blot分析.结果在静息后,RA与OA的外周血T淋巴细胞中STAT3与STAT5均处于极低水平磷酸化的状态;在IL-2作用后,T淋巴细胞中STAT3和STAT5发生时相性的酪氨酸磷酸化,而RA的T淋巴细胞磷酸化程度较OA显著增高.结论IL-2对RA患者T淋巴细胞的STAT3和STAT5过度激活,引起T淋巴细胞中IL-2信号传导的异常放大效应,可能在RA的发病过程中发挥重要作用.     

An outbreak of angiostrongyliasis in Guanging, People's Republic of China: migrants vulnerable to an emerging disease

Angiostrongyliasis has been frequently reported from the People's Republic of China during the last decade. An outbreak of angiostrongyliasis among migrant laborers in Guangning, Guangdong Province is described here. A questionnaire was developed to collect epidemiological and clinical information about 17 migrant laborers from the Bai ethnic group in Dali, Yunnan Province. Serum samples were collected and tested by enzyme-linked immunosorbent assay. Rats and mollusks from the same area where patients had collected Pomacea canaliculata were examined for presence of Angiostrongylus cantonensis. All 17 Bai migrant laborers consumed P. canaliculata and six had meningitis 3-19 days after consumption of P. canaliculata. Headache, myalgia and fatigue were the most common symptoms. Blood samples from 5 patients were positive for antibodies to A. cantonensis. The places where the migrant laborers collected P. canaliculata were identified as endemic areas for A. cantonensis. This outbreak highlights the vulnerability of migrants to angiostrongyliasis.     

钉螺对不同宿主源性日本血吸虫毛蚴的易感性

目的了解钉螺对不同终宿主源性日本血吸虫毛蚴的易感性差异。方法收集家兔、水牛和小白鼠排出的虫卵,孵出毛蚴,以毛蚴和钉螺5：1的比例感染同一地钉螺。同时设对照组,观察各组钉螺感染率、死亡率。结果实验组家兔、水牛和小白鼠排出虫卵孵出毛蚴感染钉螺,获得的阳性率分别为1.42%、8.67%和19.87%,钉螺死亡率分别为29.5%、13.5%和24.5%;对照组钉螺阳性率分别为2.63%、2.02%和11.66%,死亡率分别为24.0%、49.5%和18.5%。结论不同宿主源日本血吸虫毛蚴对钉螺易感性存在差异。     

胶囊内镜和双气囊内镜诊断小肠肿瘤及克罗恩病316例

目的:探讨胶囊内镜,双气囊内镜以及两项联合检查对小肠肿瘤及克罗恩病的诊断价值,以提高对此类小肠疾病的诊断水平.方法:316例怀疑有小肠疾病的患者中,178例行胶囊内镜检查,138例行双气囊内镜检查,32例行两项联合检查,分别对两项内镜的小肠肿瘤及克罗恩病的检出率及确诊率进行分析.结果:胶囊内镜对小肠肿瘤的检出率为4.5...     

Meta-analysis on the effect of the Ala54Thr polymorphism of the fatty acid-binding protein 2 gene on body mass index

Background and aim

The results from studies published on the association of fatty acid-binding protein 2 (FABP2) Ala54Thr polymorphism with body mass index (BMI) are conflicting. In this meta-analysis, we investigated the association of the FABP2 Ala54Thr polymorphism with BMI.

Methods and results

We searched for articles published prior to June 2009 using PubMed, HugeNavigator and China National Knowledge Internet. The languages were limited to English and Chinese. Data on BMI were collected. A pooled weighted mean difference (WMD), together with 95% confidence interval (CI), was used for this meta-analysis.A total of 27 studies with 10 974 subjects were included in this meta-analysis. The pooled effect for dominant, recessive and co-dominant model comparisons did not suggest the significant association between the FABP2 Ala54Thr polymorphism and BMI in overall populations: WMD_{fixed effects} = −0.00, 95% CI: (−0.16 to 0.15), p = 0.99, WMD_{random effects} = −0.00, 95% CI: (−0.16 to 0.15), p = 0.99, p_Q = 0.77, I² = 0%, WMD_{fixed effects} = −0.12, 95% CI: (−0.39 to 0.14), p = 0.35, WMD_{random effects} = −0.12, 95% CI: (−0.39 to 0.14), p = 0.35, p_Q = 0.47, I² = 0% and WMD_{fixed effects} = 0.07, 95% CI: (−0.11 to 0.25), p = 0.45, WMD_{random effects} = 0.07, 95% CI: (−0.11 to 0.25), p = 0.45, p_Q = 0.90, I² = 0%, respectively. The results from the comparisons of ThrThr versus AlaAla and AlaThr versus AlaAla showed no evidence that the FABP2 Ala54Thr polymorphism is significantly associated with BMI in overall populations (p > 0.05). All the results from the subgroup analyses for these genetic models comparisons were not significant (p > 0.05).

Conclusions

Our meta-analysis does not support the association between the FABP2 Ala54Thr polymorphism and BMI.     

网吧内发现的重症肺结核患者2例报告

临床资料 病例1 患者,男,20岁,咳嗽咳痰3月余,自觉发热未诊治。3 d前症状加重伴胸闷憋气,1 d前昏倒于网吧里,被急诊120送往附近医院。经双肺CT检查考虑肺结核转入我院。双肺CT示左肺毁损肺,右肺上、中、下野可见片状密度增高阴影。即时痰涂片抗酸杆菌4+。实验室检查:K 2.90,Na133,CL 96,CO219.5,BG3.8,Ca 1.13     

艾滋病合并肺结核24例分析

目的　探讨艾滋病合并肺结核的临床特点及治疗。方法 对24例艾滋病合并肺结核进行回顾性分析。结果 两病并发率由1998年的0.05%上升至2002年的0.73%。HIV感染途径为静脉吸毒(77.1%)及性途径(22.9%);临床表现有发热(91.7%),咳嗽(91.7%),咳痰(79.2%),明显消瘦(79.2%),淋巴结肿大(54.7%);胸部X线表现以继发性肺结核为主(66.7%);合并病毒性肝炎50%,痰菌阳性率41.7%,PPD试验阳性率16.7%,14例抗结核治疗(3HRZE/9HRE)治愈或完成疗程,抗结核药物不良反应发生率为79.2%。结论 艾滋病合并肺结核常发病急、症状重、PPD试验阳性率低、抗结核药物治疗不良反应多,应加强艾滋病高发区及高危人群肺结核患者并发艾滋病的警惕性。