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81.
Brenda J. Smith Bethany Hatter Karley Washburn Jennifer Graef-Downard Babajide A. Ojo Guadalupe Davila El-Rassi Robert H. Cichewicz Mark Payton Edralin A. Lucas 《Nutrients》2022,14(9)
Evidence of dried plum’s benefits on bone continues to emerge. This study investigated the contribution of the fruit’s polyphenol (PP) and carbohydrate (CHO) components on a bone model of postmenopausal osteoporosis to explore their prebiotic activity. Osteopenic ovariectomized mice were fed diets supplemented with dried plum, a crude extract of dried plum’s polyphenolic compounds, or the PP or CHO fraction of the crude extract. The effects of treatments on the bone phenotype were assessed at 5 and 10 weeks as well as the prebiotic activity of the different components of dried plum. Both the CHO and PP fractions of the extract contributed to the effects on bone with the CHO suppressing bone formation and resorption, and the PP temporally down-regulating formation. The PP and CHO components also altered the gut microbiota and cecal short chain fatty acids. These findings demonstrate that the CHO as well as the PP components of dried plum have potential prebiotic activity, but they have differential roles in mediating the alterations in bone formation and resorption that protect bone in estrogen deficiency. 相似文献
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Kurt Seetoo Maria Paz Carlos David Blythe Leena Trivedi Robert Myers Tracey England Criscelia Agee Bill Arnold Carolyn Dobbs Mary McIntyre Enrique Ramirez Julie Morita Saadeh Ewaidah Wilete Ishow Teresa Chou Kenneth Soyemi Albert E. Barskey Amy Parker Fiebelkorn Paul Lucas Emily S. Abernathy Joseph P. Icenogle Gregory S. Wallace Susan E. Reef Yoran Grant 《MMWR. Morbidity and mortality weekly report》2013,62(12):226-229
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Tereza Cristina Cardoso Camila Silva-Frade Cilene Vidovix Táparo Lucas Hidenori Okamura Eduardo Furtado Flores 《Molecular and cellular probes》2013,27(5-6):237-242
The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced in vitro and experimentally exposed to the virus. We designed an SYBR-Green-based binding assay that is quick to perform, reliable, easily optimized and compares well with the published assay. Herein we demonstrated its general applicability to detect BoHV-5 US9 gene in bovine embryos produced in vitro experimentally exposed to BoHV-5. In order to validate the assay, three different reference genes were tested; and the histone 2a gene was shown to be the most adequate for normalizing the qPCR reaction, by considering melting and standard curves (p < 0.05). On the other hand, no differences were found in the development of bovine embryos in vitro whether they were exposed to BoHV-5 reference and field strains comparing to unexposed embryos. The developed qPCR assay may have important field applications as it provides an accurate BoHV-5 US9 gene detection using a proven reference gene and is considerably less expensive than the TaqMan qPCR currently employed in sanitary programs. 相似文献
88.
Inosine exerts a broad range of antiinflammatory effects in a murine model of acute lung injury 下载免费PDF全文
Liaudet L Mabley JG Pacher P Virág L Soriano FG Marton A Haskó G Deitch EA Szabó C 《Annals of surgery》2002,235(4):568-578
OBJECTIVE: To investigate the effects of inosine on the acute lung inflammation induced by lipopolysaccharide (LPS) in vivo and on the activation and cytotoxicity elicited by proinflammatory cytokines on human lung epithelial (A549) cells in vitro. SUMMARY BACKGROUND DATA: Inosine is an endogenous purine recently shown to exert immunomodulatory and antiinflammatory effects. METHODS: Mice challenged with intratracheal LPS (50 microg) were treated after 1, 6, and 12 hours with inosine (200 mg/kg intraperitoneal) or vehicle. After 24 hours, bronchoalveolar lavage fluid was obtained to measure proinflammatory (tumor necrosis factor-alpha [TNF-alpha], interleukin [IL]-1beta, IL-6), and antiinflammatory (IL-10, IL-4) cytokines, chemokines (MIP-1alpha and MIP-2), myeloperoxidase activity and total cell counts, nitric oxide production, and proteins. Lung histology and immunohistochemical detection of 3-nitrotyrosine, a marker of nitrosative stress, were performed in inflated-fixed lungs. In vitro, cell viability and production of the chemokine IL-8 were evaluated in A549 cells stimulated with a mixture of cytokines in the presence or absence of inosine. RESULTS: Inosine downregulated the LPS-induced expression of TNF-alpha, IL-1beta, IL-6 and MIP-2 and tended to reduce MIP-1alpha, whereas it enhanced the production of IL-4. Total leukocyte counts, myeloperoxidase, nitric oxide production, and proteins were all significantly decreased by inosine. The purine also improved lung morphology and suppressed 3-nitrotyrosine staining in the lungs after LPS. Inosine attenuated the cytotoxicity and the expression of IL-8 induced by proinflammatory cytokines in A549 cells. CONCLUSIONS: Inosine largely suppressed LPS-induced lung inflammation in vivo and reduced the toxicity of cytokines in lung cells in vitro. These data support the proposal that inosine might represent a useful adjunct in the therapy of acute respiratory distress syndrome. 相似文献
89.
Schepers T Kieboom BC Bessems GH Vogels LM van Lieshout EM Patka P 《Strategies in trauma and limb reconstruction (Online)》2010,5(2):97-103
Depending upon initial treatment, between 2 and 30% of patients with a displaced intra-articular calcaneal fracture require
a secondary arthrodesis. The aim of this study was to investigate the effect of subtalar versus triple arthrodesis on functional
outcome. A total of 33 patients with 37 secondary arthrodeses (17 subtalar and 20 triple) with a median follow-up of 116 months
were asked to complete questionnaires regarding disease-specific functional outcome (Maryland Foot Score, MFS), quality of
life (SF-36) and overall satisfaction with the treatment (Visual Analogue Scale, VAS). Patient groups were comparable considering
median age at fracture, initial treatment (conservative or operative), time to arthrodesis, median follow-up, and post-arthrodesis
radiographic angles. The MFS score was similar after subtalar versus triple arthrodesis (59 vs. 56 points; P = 0.79). No statistically significant difference was found for the SF-36 (84 vs. 83 points; P = 0.67) and the VAS (5 vs. 6; P = 0.21). Smoking was statistically significantly associated with a non-union (χ2 = 6.60, P = 0.017). The current study suggests that there is no significant difference in functional outcome between an in situ subtalar
or triple arthrodesis as a salvage technique for symptomatic arthrosis after an intra-articular calcaneal fracture. Smoking
is a risk factor for non-union. 相似文献
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Layla C. Lucas Ronnie Fass Robert S. Krouse 《JSLS, Journal of the Society of Laparoendoscopic Surgeons》2010,14(4):615-618