冠状动脉粥样硬化性心脏病病人冠状动脉旁路移置术的治疗效果

①目的 探讨冠状动脉粥样硬化性心脏病(CAD)病人行冠状动脉旁路移置术的治疗效果。②方法 148例CAD病人，体外循环下行旁路移置术(CABG)62例，非体外循环下行旁路移置术(OPCAB)86例。③结果 围手术期死亡3例，8例病人使用主动脉内球囊反搏(IABP)，145例病人术后康复出院。④结论 冠状动脉旁路移置术是治疗CAD的一种安全有效的方法，在掌握手术适应证及技术条件允许情况下应尽量行非体外循环旁路移置术和全动脉化旁路移置术。     

从病情危重度与住院费用的关系看卫生服务的公平性

公平合理地分配卫生资源是制定卫生政策的重要医学伦理学原则之一。作者在对高血压患者危重度分级的基础上，通过分析患者危重度等级与住院费用的关系，揭示了住院患者的资源消耗量与实际医疗需求相脱节的现象，提示我国卫生资源分配的公平性有待进一步提高。     

全麻手术对肥胖患者血浆抵抗素和肾素血管紧张素及血糖的影响

目的　研究全麻诱导以及手术对肥胖患者血浆抵抗素 (resistin)和肾素、血管紧张素Ⅱ及血糖的影响。方法　选择 4 0例全麻手术患者 ,根据BMI指数分为两组 :A组 (BMI>2 5 ) 2 0例为肥胖者 ;B组 (2 1 相似文献   

准分子激光原位角膜磨镶术治疗近视后双眼影像不等的临床分析

目的：探讨准分子激光原位角膜磨镶术(LASIK)对近视眼患者双眼影像不等(BA)的影响。方法：采用中国人民解放军海军总医院眼科刘蔼年设计的《双眼影像不等检查图》，对64例行MSIK术的近视眼患者按双眼屈光度差的程度分为四组，术前、术后6个月分别行BA检查，并观察双眼视力与BA恢复的关系。结果：双眼屈光度差≤2.50D的患者术前、术后双眼影像不等的一、二、三类检查图的差异无显著性。屈光度差大于2.50D的患者，术前、术后双眼一、三类检查图的差异有显著性，术后双眼裸眼视力与第三类检查图的恢复密切相关。结论：LASIK术可以使双眼屈光度差值大的患者的BA减小到可以承受的范围，并有助于三级功能的恢复，同时术后视力越好，三级功能恢复越佳。眼科学报2003；19：107-109     

玻璃体切割手术治疗化脓性眼内炎

目的 观察玻璃体切割手术治疗化脓性眼内炎的疗效。方法 对1999年6月～2003年2月在我院行玻璃体切割术的14例(14只眼)化脓性眼内炎患者进行回顾性分析。除眼内异物外，其余患者临床诊断化脓性眼内炎后均先采用保守治疗，密切观察1～2天无好转而行玻璃体切割手术。眼内炎的原因：眼球穿通伤13只眼，青光眼术后晚期滤过泡感染1只眼。玻璃体切割手术前先抽取前房及玻璃体腔内液体涂片及培养。采用标准三通道闭合式玻璃体切割手术，7只眼因前房积脓及晶状体前大量渗出和3只眼外伤性白内障影响手术而同时切除晶状体，8只联合巩膜环扎。缝合巩膜切口后玻璃体腔内注射罗氏芬2．5mg及地塞米松0．4mg。术后全身继续使用抗生素及激素1周。术后平均随访14．8个月(2～24个月)．结果 14只眼中术后视力提高12只眼(85．7％)，眼球保留13只眼(92．6％)。5只眼培养阳性。术后玻璃体腔内少量出血2只眼，无其它手术并发症。结论 玻璃体切割手术联合眼内注射抗生素治疗化脓性眼内炎疗效可靠。只要手术时机选择适当，大多数患者能保住眼球，恢复有用视力。     

Optical Pretargeting of Tumor with Fluorescent MORF Oligomers

Objective Pretargeting with radioactivity has significantly improved tumor to normal tissue radioactivity ratios over conventional antibody imaging in both animal studies and clinical trials. This laboratory is investigating DNA analogues such as phosphorodiamidate morpholinos (MORFs) for pretargeting using technetium-99m (^99mTc) for detection. However, the unique properties of fluorescence activation and quenching combined with oligomers with their unique properties of hybridization may be particularly useful when used together for pretargeting with optical detection. The use of linear fluorophore-conjugated oligomer duplexes have been little used in animals, and to our knowledge, have not previously been considered for pretargeting applications. Methods A MORF/cDNA pair was selected such that when hybridized, the fluorescence of the Cy5.5-conjugated 25 mer MORF (Cy5.5–MORF25) is inhibited with a BHQ3-conjugated 18 mer complementary DNA (BHQ3–cDNA18). The short BHQ3–cDNA18 was selected to dissociate in the presence of a long cMORF25 in the pretargeted tumor, thus releasing the inhibitor from the Cy5.5 emitter. In this manner, the Cy5.5 fluorescence will be inhibited everywhere but in the target. The dissociation was first examined in vitro by adding the duplex to the cMORF25 both in solution and immobilized on polystyrene microspheres and by surface plasmon resonance (SPR). Thereafter, biotinylated cMORF25 immobilized on streptavidin polystyrene microspheres were administered intramuscularly in one thigh of hairless SKH-1 mice as target while an identical weight of the identical microspheres but without the cMORF25 was administered in the contralateral thigh as control. The animals then received IV the Cy5.5–MORF25/BHQ3–cDNA18 duplex or equal molar dosage of single-chain Cy5.5–MORF25 and were imaged. Results The SPR studies showed that the immobilized cDNA18 rapidly captured the flowing MORF25 to provide a duplex with a slow dissociation rate constant. Furthermore, when cMORF25 was next allowed to flow over the now immobilized duplex, the cDNA18 was unable to prevent dissociation of the heteroduplex and the formation and release of the cMORF25-MORF25 homoduplex. Images of animals obtained soon after receiving the Cy5.5–MORF25 singlet showed intense whole body fluorescence obscuring the target thigh. However, only 5 minutes after receiving the Cy5.5–MORF25/BHQ3–cDNA18 duplex, the target thigh was clearly visible along with only the kidneys. Conclusions This first study of optical pretargeting provides a proof of concept that oligomer pretargeting found to be useful with radioactivity detection is applicable with fluorescent detection as well. In addition, our results demonstrate that by using linear oligomers for optical pretargeting, chain lengths (and base sequences) may be manipulated to provide duplexes with stabilities and fluorescence inhibition optimized for pretargeting and other in vivo applications of optical imaging.     

尿脱落细胞端粒酶检测在膀胱癌中的诊断价值

目的　利用聚合酶链反应的酶联免疫吸附试验 ,探讨检测自排尿脱落细胞端粒酶活性在膀胱癌早期诊断中的意义。方法　应用 (端粒酶重复序列扩增基础上PCR -ELISA)TRAP -PCR -ELISA方法检测 5 8例膀胱癌患者、 2 5例良性血尿患者及 30例健康体检者尿脱落细胞的端粒酶活性。结果　 (1) 5 8例膀胱癌患者尿脱落细胞阳性率为 81 0 % (4 7/ 5 8) ,2 5例良性血尿患者及健康体检者均无端粒酶活性表达。 (2 )TRAP -PCR -ELISA方法检测膀胱癌患者尿脱落细胞端粒酶活性与常规尿细胞形态学镜检间的差别有非常显著性意义 (P <0 0 1)。结论　尿脱落细胞端粒酶活性的检测法对膀胱癌患者的阳性检出率较常规尿细胞形态学方法高 ,可用于膀胱癌的早期诊断。     

口腔粘膜癌前病变及鳞癌角蛋白表达的免疫组化研究

目的 研究口腔粘膜标本中角蛋白的表达与分布。方法 利用多克隆抗角蛋白抗体Ａ５７５，采用免疫组化技术。结果 从正常口腔粘膜，轻、中、重度异常增生到鳞癌角蛋白表达显著增强，Ａ５７５在所有鳞癌标本中均为阳性，但染色强度深浅不一，且随鳞癌分化程度降低而降低，染色弱的病例其临床分期高，更易发生淋巴结转移。结论角蛋白表达的增强在强人上皮细胞中的致癌基因上起作用，这种强化的积累将加速癌前疾病的进展，因此，Ａ５７     

42.3GHz毫米波电场治疗大鼠脊髓损伤的实验研究

研究毫米波对损伤脊髓修复的生物效应。方法采用频率４２．３ＧＨｚ，波长７．１ｍｍ，同功率密度２ｍＷ／ｃｍ＾２，７ｍＷ／ｃｍ＾２，２０ｍＷ／ｃｍ＾２的毫米波治疗大鼠损伤脊髓，３０ｍｉｎ，２次／ｄ＊２０，观察组织学及皮层体感诱发电位、运动诱发电位变化。