阿片类药物透过血脑屏障的三维构效研究

目的:建立药物透过血脑屏障的三维构效模型,为药物分子设计提供理论依据。方法与结果:利用比较分子力场分析方法建立了阿片类药物透过血脑屏障的三维定量构效模型,该模型有较高的预测能力,交叉验证系数r²_cv=0.718,相关系数r²=0.978,F₃,7=67.902,标准偏差SE=0.209。结论:根据CoMFA模型系数等势图,解释了该类药物透过血脑屏障的构效关系。     

开展运动相关损伤的影像学研究

随着2008年北京奥林匹克运动会日益临近,我国掀起了全民健身高潮,运动医学的发展也越来越为广大医学工作者所重视.从1928年国际运动医学联合会(FIMS)成立到1978年中国运动医学会成立,再到2008年的奥运年,运动医学的发展非常迅速,涵盖的内容和服务的对象越来越广.     

尿2-硫代噻唑烷-4-羧酸用于二硫化碳接触生物监测研究

应用高效液相色谱法分析尿中2-硫代噻唑烷-4-羧酸(TTCA)。54名非接触者的结果示个体差异较大,频数分布呈明显偏态,几何平均水平约0.06mg/g肌酐。9名非接触者的动态观察表明,同一个体有时检出、有时检测不出,每天排泄总量平均0.046mg,未见明显规律。接触二硫化碳工人班前尿TTCA排泄特征与非接触者一样,几何平均水平0.08mg/g肌酐;不同接触水平者次日班前尿、工作周连续每日班前尿TTCA均无明显差异,提示尿TTCA无蓄积性,接触后16h能完全排出体外;接触者班未尿TTCA明显上升,呈现群体接触效应关系。取生物监测临界值0.3mg/g肌酐,尿TTCA反映二硫化碳接触特异度、敏感度和阳性预测值分别是87％、84％和86％。可用于二硫化碳接触的群体生物监测。     

NHS-MAS3: a bifunctional chelator alternative to NHS-MAG3.

This laboratory uses an N-hydroxysuccinimide derivative of S-acetylmercaptoacetyltriglycine (NHS-MAG3) to conjugate amines for subsequent labeling with 99mTc. However, the synthesis from triglycerine is general and not restricted to this tripeptide. We had earlier selected a small number of alternative tripeptides and synthesized the corresponding NHS derivatives. Each was then evaluated in a search for bifunctional chelators with properties superior to NHS-MAG3, such as lower serum protein binding or improved stability to cysteine challenge. Based on these preliminary results, NHS-S-acetylmercaptoacetyltriserine (NHS-MAS3) was selected for further investigation. We have now conjugated this bifunctional chelator to an biocytin and to an amine-derivatized peptide nucleic acid (PNA). Both carriers were also conjugated with NHS-MAG3 under identical conditions and all were labeled with 99mTc at neutral pH and at boiling temperature while the conjugated PNAs were radiolabelled at neutral pH and at room temperature. Regardless of the chelator, reverse phase HPLC radiochromatograms of the labeled biotins and PNAs after purification showed a single peak. However, by size exclusion HPLC, the radiochromatograms always showed several peaks even after purification, but the MAS3 radiochromatograms were less complicated. For biotin and PNA both, radiolabeling via MAS3 showed improved 99mTc stability in 37 degrees C serum and in cysteine solution. The four preparations were administered to mice implanted in one thigh with avidin beads (biotins) or complementary PNA beads (PNAs). At 5 h post-administration, no significant differences were observed in the targeting of PNA beads between the two chelators, however the target thigh/normal thigh ratio was significantly higher for MAS3-biotin compared to MAG3-biotin. We conclude that labeling biocytin and amine-derivatized PNA with NHS-MAS3 compared to NHS-MAG3 provides simpler radiochromatographic profiles, improved stability of the label in serum and cysteine solution and can improve in vivo targeting.     

财政投入方式应如何规范

《中共中央、国务院关于卫生改革与发展的决定》中指出:“加快制定卫生机构设置、人员编制标准,规范财政对卫生机构的投入。”国务院办公厅转发的由国务院体改办等八部门联合制定的《关于城镇医药卫生体制改革的指导意见》也就财政对卫生机构的补助范围和方式提出了要求。     

偏振光游标式调节检测仪的研制

介绍一种根据Scheiner氏盘原理研制的偏振光游标式调节检测仪 ,专用于人眼张力性调节、调节反应和远、近点的测量。通过临床检测 ,该仪器参数稳定 ,重复性好 ,反应灵敏 ,为与眼动参数有关的近视眼研究领域提供了可靠的实验方法和设备。     

The extent and pattern of infiltration along the bronchial wall in central type lung cancer

Gross and microscopic study was made in fifty specimens emphasizing on the bronchial wall of lung cancer. The extent and pattern of infiltration along the proximal bronchial wall in the central type lung cancer were observed. The cancerous tissue extended proximally from less than 1.2 cm in the majority of cases, to a maximum of 1.6 cm from the cancer base. The lesions can be divided into three gross types: infiltrating, polypoid and nodular. In the infiltrating type, the cancerous tissue spread along the bronchial wall far and may involve multiple layers in the wall. In the polypoid type, the infiltration did not reach far as compared with the other types. None in the polypoid type was over 0.4 cm. The nodular type comprising 54% (27/50), was more common than the others. In order to improve the operative result, infiltrating pattern and distance along the bronchial wall are important references for resection of central type lung cancer.     

用免疫微球荧光测定法检验C_1型葡萄球菌肠毒素的研究

用 NalO_4醛化 Sepharose CL4B 微球。将兔抗 C_1 型葡萄球菌肠毒素抗体(兔抗SEC_1抗体)偶联到醛化的 Sepharose CL4B 微球上,制得 Sepharose CL4B 免疫微球。我们用 FITC 示标的兔抗 SEC_1抗体作示踪溶液,用上述免疫微球检验 SEC_1,用光学纤维显微荧光计测量标本的荧光发射。方法的检测敏感度为156ng SEC_1/ml。该方法也可用于检测其他可溶性抗原或抗体。     

增殖细胞核抗原在肾盂输尿管移行细胞癌中表达的临床意义

目的：通过免疫组化方法研究增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）在肾盂输尿管移行细胞癌中表达的临床意义。方法：１９７６年８月—１９９１年８月收治存档的上尿路移行细胞癌组织蜡块３９例行免疫组化分析。采用Ｓ－Ｐ法，一抗为ＰＣ１０，膀胱移行细胞癌切片做阳性对照，肾盂积水切片做阴性对照。每张切片计数五个视野，求出ＰＣＮＡ指数。ＰＣＮＡ指数与临床分期、病理分级间比较用方差分析，多变量生存分析用Ｃｏｘ模型。结果：ＰＣＮＡ阳性染色为肿瘤细胞核染成棕黄色，分布不均，与对照片对比鲜明。染色阳性细胞数目、着色深度与分期分级关系密切。ＰＣＮＡ指数截断点定为２５％。≤２５％与＞２６％两组术后五年生存率差异显著。Ｃｏｘ模型表明ＰＣＮＡ指数是非常重要的一项预后指标。结论：ＰＣＮＡ是一个有临床意义的细胞增殖标记物。ＰＣＮＡ提供的细胞动力学信息反映了肿瘤的生物学行为。ＰＣＮＡ指数是一个客观的定量指标。ＰＣＮＡ指数与分期分级关系密切，可作为分期分级的一种辅助方法。