c-erbB2蛋白在大鼠睾丸中的分布

用免疫组织化学方法观察基因基白c-erbB2在大鼠睾丸中的分布。结果：未成年大鼠睾丸生精上皮细胞和间质细胞中均未见c-erbB2免疫染色阳性颗粒,成年大鼠睾丸曲细精管中的圆形精子细胞,精子和残体均可见c-erbB2蛋白存在。提示原癌基因c-erbB2的活性可能参与精子形成的调控。     

COMPLICATIONS IN LAPAROSCOPIC GYNECOLOGIC SURGERY

Objective. To investigate retrospectively the complications and associated factors of gynecological laparescopies.Methods. 1769 laparoscopic surgeries were carried out from January 1994 to October 1999 at our department. The procedures included 1421 surgeries of ovary and tube, 52 myomectomies and 296 cases of laproscopic-assisted vaginal hysterectomy (LAVH). A total of 312 patients had a history of prior laparotomy (17.6%). Results. Complications occured in 34 cases, the overall complication rate was 1.92%. Unintended laparotomies occured in 6 cases(0.34% ). 12 complications were associated with insertion of Veress needle or trocar and creation of pneumoperitoneum, including 5 severe emphysema and 7 vascular injuries, this figure represents 35.3% of all complications of this series. Five intraopemtive complications (14.7 % ) occured during the laparescopic surgery (3 severe bleedings, one bladder injury and one skin bum of leg caused by damaged electrode plate), laparotomy was re-quired in four of these cases. Seventeen complications occured during postoperative stage: 2 intraperitoneal hemor-rhages needing laparotomy, 2 bowel injuries, 4 nerve paresis and 9 febrile morbidities. Cordusions. Operative gynecologic laparoscopy is associated with acceptable morbidity rate, but can not be over-looked. Complication rate seems to be higher in advanced procedures such as LAVH.     

Human papillomaviruses and papillomatosis lesions of the female lower genital tract

Objective: The objective of this study was to determine whether human papillomavirus (HPV) infections are involved in the development of papillomatosis lesions of the lower female genital tract.Methods: A total of 616 biopsy specimens of genital papillomatous lesions (307 nodular and 309 papular types) from 598 patients were anaylyzed for the presence of HPV DNA sequences by polymerase chain reaction (PCR). These specimens were also examined by histopathological assessment for characteristic HPV-associated cytological changes, by immunohistochemical staining for HPV-associated antigen, and by electron microscopy for the presence of virions.Results: HPV DNA sequences were found in 97.9% (140 of 143 cases) and 1.1% (1 of 91 cases) of the nodular and papular papillomatosis cases tested, respectively. In 18 patients who had both types of papillomatosis lesions, HPV DNA was invariably found only in nodular tissues. HPV-associated antigen, koilocytosis, and virions were found in 53.6% (98 of 183 cases), 70.5% (129 of 183 cases), and 5.9% (5 of 85 cases) of nodular papillomatosis lesions tested, respectively.Conclusions: These data suggest that nodular papillomatosis was closely associated with HPV infection, but that papular papillomatosis of the lower female genital tract may have an etiology other than HPV infection.     

4种灭蝇毒饵灭蝇效果观察

目的:观察4种苍蝇毒饵的灭蝇效果。方法:在0.343m~3的测试柜内观察苍蝇的LT_(50)及24h死亡率。结果:蝇蟑宁和韩1号毒饵的半数死时间最短,分别为1.10和1.79h,24h死亡率最高分别为98.43％和91.41％,两者差异无显著性。结论:蝇蟑宁毒饵灭效最好,具有推广应用价值。     

成釉细胞纤维肉瘤间叶细胞的电镜和免疫组化研究

为探讨成釉细胞纤维肉瘤（ Ａ Ｆ Ｓ）间叶细胞的本质及特征, 作者采用免疫组化和电镜技术,对３例 Ａ Ｆ Ｓ间叶细胞进行了研究,并与６例成釉细胞纤维瘤进行比较。结果显示： Ａ Ｆ Ｓ的间叶细胞主要由成纤维细胞构成,此外尚有少许未分化间叶细胞和组织细胞, 偶见肌纤维母细胞。成纤维细胞仅表现 Ｖｉｍ ｅｎｔｉｎ 阳性。肌纤维母细胞呈 Ｖｉｍ ｅｎｔｉｎ, Ｈ Ｈ Ｆ３５及 α Ｓ Ｍ Ａ 阳性。组织细胞 Ｐ Ｇ Ｍ１ 和ｋｐ１标记均阳性, 可能是炎症或免疫反应的产物。与成釉细胞纤维瘤比较, Ａ Ｆ Ｓ间叶细胞具备较高的 Ｐ Ｃ Ｎ Ａ 阳性标记率, 提示 Ａ Ｆ Ｓ的增生活性较高。     

Reconstructive surgery for urinary incontinence

The clinical urologist often is faced with the referral of a patient with urinary incontinence refractory to conservative measures. Given the broad spectrum of causes of urinary incontinence, the clinician must base evaluation and therapeutic management on current principles of urinary tract pathophysiology. This article organizes the pertinent diagnostic considerations that must be addressed in guiding the clinician to the appropriate surgical treatment option.     

结肠、直肠癌术前区域动脉灌注化疗的临床病理观察

目的 探讨结直肠癌术前区域动脉灌注化疗对临床病理特征的影响及其可行性。方法 对３０例结、直肠癌患者以Ｓｅｌｄｉｎｇｅｒ方法插管、行选择性区域动脉并行灌注化疗。结果 所有病例病理检查均发现沿血管周围的癌细胞发生核固缩、碎裂,胞浆凝固、坏死、细胞间质水肿、纤维增生、炎细胞浸因和出现内膜增生、血管炎及血栓形成。结论订前选择性区域动脉灌注化疗要明显发迹结直肠癌患者的组织学形态,并使患者临床症状改善,对结直     

Epidemiological study on HIV/AIDS in Cambodia seroprevalence of HIV/STD among commercial sex workers

OBJECTIVE: To describe epidemiological features of HIV prevalence among female commercial sex workers (CSWs) in Cambodia, a cross-sectional study using a questionnaire study and serological tests was carried out from December 1997 to January 1998. We report the main results of the analyses of serological tests in this article. METHODS: Two hundred ninety six CSWs working in Sisophon and Poi Pet, located in northwest Cambodia, Bantey Mean Chey province, were recruited for interview based on a questionnaire on sexual behavior, and serological tests. The blood samples were examined for HIV antibody, Chlamydia trachomatis IgG antibody, TPHA, Hepatitis B surface antigen, and Hepatitis B surface antibody. The relationship between HIV and the other STD's was analyzed by using logistic regression analysis. RESULTS: The HIV seroprevalence rate was 43.9% (130 out of 296). The seropositive rate of Chlamydia trachomatis IgG antibody (C.T.-IgG-Ab) was 73.3% (217 out of 296). Logistic regression analysis showed a significant association between C.T.-IgG-Ab positive and HIV prevalence. (Odds Ratio: 5.33; 95% Confidence Interval, 2.82-10.07). CONCLUSIONS: This study suggests that the existence of Chlamydia trachomatis is closely related with HIV prevalence among CSWs in Cambodia. Other STDs may also increase susceptibility to male-to-female sexual transmission of HIV. This suggests that appropriate prevention against STDs will be needed for the control of HIV prevalence in Cambodia.     

急性非淋巴细胞白血病中MGMT与p53基因突变的研究

目的 探讨肿瘤抑制基因ｐ５３、ＭＧＭＴ在急性非淋巴细胞白血病（ＡＮＬＬ）中的改变。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术对３１例ＡＮＬＬ患者、２２例正常人ｐ５３基因第５、６、７、８外显子和ＭＧＭＴ基因第１、４外显子进行突变分析。结果 ３１例ＡＮＬＬ中５例存在ｐ５３基因突变、４例存在ＭＧＭＴ基因突变,其中２例ｐ５３与ＭＧＭＴ同时发生突变。结论 ｐ５３基因突变在白血病发生中起一定     

长期饮酒对男性生殖系统功能影响

目的探讨饮酒对男性生殖系统的损害及其机理。方法选择45名男性饮酒者和9名慢性酒精中毒者为研究对象,以15名不吸烟不接触毒物的男性为对照组。结果长期大量饮酒和酒精中毒者血清中T和CS水平下降,而LH和FSH水平升高;精浆和精子中LDH,LDH-x和G-6PD活力下降,以LDH-x下降为敏感;精子数量和活动精子年均降低,精子畸形率及异常精液检出率均升高。上述各指标与对照组比较差异均有高度显著性（P＜0.01）。结论长期饮酒可损害男性生殖系统功能。