Identification of estrogen-associated intrinsic aging genes in Chinese Han female skin by cDNA microarray technology

Objective Estrogens play an important role in intrinsic skin aging.The associated changes in global gene expression are poorly understood.Methods We used the Illumina microarray platform to obtain comprehensive gene expression profiles in female Chinese Han skin,and confirmed the data by quantitative real-time PCR (Q-RT-PCR).Results We found 244 genes significantly related to estrogen-associated intrinsic skin aging,and some of these genes were confirmed by Q-RT-PCR.We also performed functional analysis by ...     

低压低氧对大鼠下丘脑Orexin A表达及舌下神经放电的影响

目的:观察慢性间歇性低压低氧(chronic intermittent hypobaric hypoxia,CIHH)模型大鼠下丘脑OrexinA表达及舌下神经放电活动,探讨CIHH对呼吸活动调节的机制。方法:对正常成年雄性SD大鼠进行每天6 h的低压低氧处理,28 d后进行血气分析,采用免疫组织化学染色方法观察大鼠下丘脑Orexin A的表达,记录舌下神经放电作为观察呼吸活动的指标。结果:血气分析显示模型组大鼠的PO2下降,PCO2明显升高(P<0.05,n=6);模型组大鼠下丘脑Orexin A免疫阳性神经元的相对光密度值显著升高(P<0.01,n=5);舌下神经放电幅度积分面积减小(P<0.01,n=6),呼吸频率降低(P<0.05,n=6)。结论:CIHH使大鼠动脉血PO2下降、PCO2明显升高,舌下神经放电活动减弱。下丘脑Orexin A神经元表达的增加提示Orexin的确参与了对呼吸活动的调节,尤其在CIHH时发挥了重要作用。     

Synthesis of novel cyclodextrin-modified reduced graphene oxide composites by a simple hydrothermal method

Cyclodextrin (β-CD)-functionalized reduced graphene oxide was successfully synthesized by a simple hydrothermal method, followed by conjugating with polyethylene glycol (PEG) and folic acid (FA). Microscopic and spectroscopic techniques were used to characterize the nanocomposites. Photothermal experiments showed that β-CD-functionalized reduced graphene oxide exhibited higher photothermal conversion efficiency in the near infrared region than reduced graphene oxide functionalized with other molecules under the same conditions. Cytotoxicity experiments indicated that rGO@CD@PEG@FA possessed good biocompatibility even at high concentration. When doxorubicin (DOX) was loaded on the rGO@CD@PEG@FA nanocomposite, it showed the stimulative effect of heat, pH response, and sustained drug release. Cytotoxicity experiments also confirmed the targeted effect and high efficiency of the combined therapy. The findings of the present study provide an ideal drug delivery system for malignant cancer therapy due to the advanced synergistic chemo-photothermal targeted therapy and good drug release properties.

The rGO@CD@PEG@FA nanocomposite showed the stimulative effect of heat, pH response, and sustained drug release for cancer therapy     

30例免疫低下与肺结核

目的：分析免疫低下患合并肺结核的临床特点。方法：对我科1995－2001年收治的免疫低下合并肺结核病的30例患进行回顾性分析，结果：（1）与服用糖皮质激素及其他免疫抑制剂有关。（2）易延误诊断。（3）愈后差，死亡率高，结论：对免疫低下的患尤其是长期应用糖皮质激素，要注意肺结核的发生，提高早期确诊率。     

The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus

A newly emerged tembusu virus that causes egg-drop has been affecting ducks in China since 2010. Currently, no vaccine is available for this disease. A live attenuated duck enteritis virus (DEV; a herpesvirus) vaccine has been used routinely to control lethal DEV in ducks since the 1960s. Here, we constructed two recombinant DEVs by transfecting overlapping fosmid DNAs. One virus, rDEV-TE, expresses the truncated form of the envelope glycoprotein (TE) of duck tembusu virus (DTMUV), and the other virus, rDEV-PrM/TE, expresses both the TE and pre-membrane proteins (PrM). Animal study demonstrated that both recombinant viruses induced measurable anti-DTMUV neutralizing antibodies in ducks. After two doses of recombinant virus, rDEV-PrM/TE completely protected ducks from DTMUV challenge, whereas rDEV-TE only conferred partial protection. These results demonstrate that recombinant DEV expressing the TE and pre-membrane proteins is protective and can serve as a potential candidate vaccine to prevent DTMUV infection in ducks.     

基于超说明书用药潜在安全隐患的思考及管理对策

目的：加强超说明书用药管理以确保用药安全.方法：结合具体实例,对临床上超说明书用药存在的安全隐患进行分析与思考.结果：超说明书用药有时会引发医疗纠纷,医师应做到有章可循.结论：医院应建立严格的超说明书用药管理制度,以保障病人用药安全.     

超敏C反应蛋白对慢性阻塞性肺疾病患者病情的评估价值

目的:比较慢性阻塞性肺疾病(COPD)患者不同病期(急性加重期和稳定期)的血清超敏C反应蛋白(hs-CRP)水平,分析其在COPD病情评估中的意义。方法:选择COPD患者40例,分别在急性加重期和稳定期测定血清hs-CRP、ESR、WBC水平。hs-CRP测定方法采用胶乳免疫增强比浊法。结果:COPD患者急性加重期血清hs-CRP水平较稳定期明显上升,差异有显著统计学意义(P<0.01)。WBC、ESR变化则较小。结论:hs-CRP可作为评估COPD病情的敏感指标。     

《温病条辨》对热病针刺的认识

通过吴鞠通《温病条辨》对热病针刺的认识分析,阐述了《温病条辨》在《内经》、《金匱要略》的基础上丰富了对热病针刺的一些认识,认为“益阴以留阳,实刺法之所短”,“刺法能泄能通,开热邪之闭塞最速”,对“热入血室”刺期门“针力比药力尤捷”,这和他所制定三焦温病治疗原则,治疗温病注重养阴的学术观点是一致的。     

颈椎病患者血液流变学变化的初步探讨（附65例临床报告）

为了探讨颈椎病与血液流变血的关系，本文对６５例颈椎病患者进行了血液流变学有关项目的检测，其结果表明七项指标均有不同程度的增高，尤以血浆粘度和纤维蛋白原增高较为明显，说明改变，引起微循环障碍而发展。     

应用肾图代谢动力学参数分析利尿剂肾图对上尿路梗阻的诊断

目的探讨用数学模型推理肾脏分泌系数与排泄系数分析利尿剂肾图,以助于上尿路梗阻的诊断。方法建立邻碘马尿酸钠(OIH)在体内代谢的数学模型,用肾图代谢动力学参数绘制典型肾图曲线,对65例病人,涉及的77侧肾盂、输尿管在标准肾图检查的基础上,行利尿剂肾图检查,对照二者的诊断准确性,并行χ2检验。结果用数理方法推理出的肾图代谢动力学参数绘制的典型肾图曲线,部分曲线在b段是相同的,但代谢动力学参数有差异。标准肾图诊断上尿路梗阻的准确性为52%,假阳性为48%,无假阴性;利尿剂肾图诊断上尿路梗阻的准确性为81%,假阳性为13%,假阴性为6%;二者相差显著(P<0.01)。结论利尿剂肾图诊断上尿路梗阻的准确性显著高于标准肾图,但仍存有假阳性与假阴性,其原因主要是由于OIH进入肾脏的速度与排出速度的比率变化所致。用肾图代谢动力学参数分析利尿剂肾图可有机地将肾脏的分泌与排泄功能结合起来观察,更新了以往肾图分析的某些概念,使肾图分析方法从静态定性转到定量分析。