Maintenance to target was associated with radiological outcomes in patients with rheumatoid arthritis: a real-world observational cohort study

Clinical Rheumatology - To investigate the effect of different maintenance to target on radiologic outcomes in patients with rheumatoid arthritis (RA) in real-world setting. RA patients enrolled...     

Synergistic activity of ABT-263 and ONC201/TIC10 against solid tumor cell lines is associated with suppression of anti-apoptotic Mcl-1, BAG3, pAkt,and upregulation of pro-apoptotic Noxa and Bax cleavage during apoptosis

A major underlying cause of the resistance of solid tumor cells to cancer therapy is the evasion of cell death following anti-cancer drug treatment. We explored the combination of TRAIL-inducing compound ONC201/TIC10 and Bcl-xL/Bcl-2 inhibitor ABT-263 to target the extrinsic and intrinsic apoptotic pathways, respectively, in solid tumor cell lines (N = 13) derived from different tissues (colon, prostate, lung, breast, ovary, bladder). We found an IC50 range of 0.83-20.10 μM for ONC201 and 0.06-14.75 μM for ABT-263 among the 13 cancer cell lines. We show that combination of ONC201 and ABT-263 produces a strong synergistic effect leading to tumor cell death, and that the combination is not toxic to human fibroblast cells. In OVCAR-3 ovarian cancer cells, 2.5 μM ONC201 and 1.25 μM ABT-263 yielded 37% and 27% inhibition of viability, respectively, while the combination of the two agents yielded 92% inhibition of viability, resulting in a high synergy score of 52; conversely, the same combination in the HFF-1 human fibroblast cells yielded 2.45% inhibition of viability and a synergy score of 6.92 (synergy scores were calculated using SynergyFinder; scores greater than 10 are considered synergistic). We also found that the combination of these two agents resulted in synergistic caspase activation and PARP cleavage consistent with induction of apoptosis. Combination therapy-induced cell death correlated with decreased levels of Mcl-1, BAG3, pAkt, and upregulation of Noxa along with Bax cleavage during apoptosis at 48 hours, and ATF4, TRAIL, and DR5 induction at 24 hours. There was some heterogeneity in the cell lines with regard to these responses. Our data provide evidence for synergy from the combination of ONC201 and ABT-263 against human solid tumor cell lines associated with alterations in cell death and pro-survival mediators. The combination of ONC201 and ABT-263 merits further exploration in vivo and in clinical trials against a variety of solid malignancies.     

Syncytin和GCMa在特发性胎儿生长受限胎盘中的表达

目的探讨Syncytin及glial cells missing(GCMa)在特发性胎儿生长受限(idiopathic fetal growth restriction,IFGR)患者胎盘组织中的表达及意义。方法选取剖宫产分娩的IFGR孕妇32例为研究组,同期因社会因素剖宫产分娩的健康足月孕妇30例作为健康对照组。采用免疫组化S-P法和逆转录聚合酶链反应(RT-PCR)检测2组胎盘组织中Syncytin和GCMa的表达。结果免疫组化及RT-PCR结果均显示Syncytin和GCMa在研究组胎盘组织中表达水平低于健康对照组,差异均具有显著统计学意义(P<0.01)。Syncytin及GCMa在研究组胎盘组织中表达均降低,两者呈正相关(r=0.537,P<0.05)。结论 Syncytin与GCMa在IFGR胎盘组织中均低表达,提示可能在该疾病的发生、发展过程中起重要作用。     

中药新药研发中原料药材调查研究思路和方法——以巴天酸模为例

目的：探讨地方标准收载药材作为新药研发原料药材资源的研究方法。方法：查阅资料和标本,分析确定出主要分布区,制定调查路线;进行野外初查,制定适合调查品种的野外调查方案。结果和结论：根据资源分布和数量调查结果确定出资源量集中的区域,采集用于质量研究的样本,归纳出影响药材质量的一些因素。     

1例肥胖症患者腹腔镜下行可调节胃束带减容术的护理

对1例肥胖症患者腹腔镜下行可调节胃束带减容术，结果手术效果满意。提出术前正确评估患者病情，加强心理护理，完善术前准备，术后严密监测生命体征，预防脑梗死、肺栓塞等并发症发生，是保证手术成功的关键。     

Protective Effect of Extract of Folium Ginkgo on Repeated Cerebral Ischemia-Reperfusion Injury

Protective Effect of Extract of Folium Ginkgo on Repeated Cerebral Ischemia-Reperfusion Injury@周兰兰 @明 亮 @江 勤 @马传庚     

声门下冲洗引流在预防VAP中的作用

目的:观察可冲洗气管导管在预防呼吸机相关性肺炎中的作用,并探计相应的护理措施。方法:将收入ICU需行机械通气的病人随机分为二组,采用可冲洗气管导管接受声门下积液冲洗为引流组,采用普通气管导管为常规治疗组,定期从口咽部、声门下分泌物和下呼吸道获取细菌学样本,记录行机械通气的天数和发生VAP的病例数结果。常规治疗组VAP发病率为53.3％,平均发生时间8.4±3.0d积液引流组VAP发病率为25.7％,平均发生时间11.6±4.1d经统计学处理P<0.05,二组结果有显著性差异。结论:声门下积液的仔在是引起机体发生VAP的主要原因之一,应用可冲洗气管导管行声门下积液冲洗引流可以明显减少VAP的发生率,是一种简单而有效的方法。     

血管周上皮样细胞肿瘤的病理诊断及新进展

概述血管周上皮样细胞肿瘤（PEComa）概念演进及2020版WHO软组织肿瘤分类中关于该肿瘤的更新;总结并归纳PEComa的临床病理学特征、主要鉴别诊断及诊断陷阱、良恶性诊断标准等;详述累及区域淋巴结的良性血管平滑肌脂肪瘤（AML）;阐述PEComa的分子遗传学研究和靶向药物治疗等方面新进展,为临床治疗策略提供新思路。     

壳聚糖水凝胶与星形胶质细胞体外生物相容性评价的实验研究

研究壳聚糖水凝胶材料与星形胶质细胞的体外生物相容性,初步探讨壳聚糖水凝胶作为神经组织工程支架材料的可行性。利用氯化壳聚糖、β-甘油磷酸钠和羟乙基纤维素制备壳聚糖水凝胶,MTT法评价其细胞毒性;体外培养鉴定新生Wistar大鼠脑皮层星形胶质细胞;壳聚糖水凝胶与星形胶质细胞体外共培养,观察星形胶质细胞在材料上的生长;MTT法检测接种后1、35、、7d的细胞增殖度。体外成功制备壳聚糖水凝胶,该材料无细胞毒性;体外分离、培养得到状态良好的星形胶质细胞;体外星形胶质细胞在材料上培养呈星形,生长良好,有分支状突起形成;MTT结果表明,材料-细胞共培养组中的细胞增殖度明显高于单纯细胞组（P〈0.001）。壳聚糖水凝胶与星形胶质细胞在体外具有良好的生物相容性,有望作为神经组织工程支架材料。