人S100 A9重组腺病毒载体的构建和鉴定

目的制备人S100A9(hS100A9)重组腺病毒载体,为进一步研究人S100A9蛋白的分子生物学作用奠定基础。方法从pHAHA-hS100A9中PCR扩增hS100A9片段,亚克隆至穿梭质粒pAdTrack-TOX,获得重组穿梭质粒pAdTrack-TOX-hS100A9。酶切、聚合酶链反应(PCR)及测序鉴定正确后经PmeⅠ酶切电转入AdEasier感受态细胞,获得重组腺病毒质粒pAdhS100A9,该质粒经PacⅠ酶切后由脂质体转染至HEK293细胞中包装扩增重组腺病毒并进行滴度测定,最后通过PCR及蛋白印迹法(Western blot)鉴定重组腺病毒。结果正确扩增出hS100A9基因;重组穿梭质粒pAdTrack-TOX-hS100A9均及重组腺病毒质粒pAdhS100A9构建成功,并在HEK293细胞中成功包装且扩增后滴度为1 010 I U/mL;重组腺病毒AdhS100A9在HEK293细胞中成功转录和表达。结论用pAdEasy系统成功构建hS100A9重组腺病毒载体,为探讨其生物学作用奠定了基础。     

阻塞性睡眠呼吸暂停综合征对肝脏酶谱影响的配对研究

目的:评价阻塞性睡眠呼吸暂停综合征(obstructive sleep apnea syndrome,OSAS)对肝脏酶学的影响。方法:选择30例按照体重指数配对的不同程度OSAS男性患者,进行睡眠呼吸暂停监测,并于清晨空腹抽血测定空腹血糖,空腹胰岛素水平,血清丙氨酸氨基转移酶、天门冬氨酸氨基转氨酶及乳酸脱氢酶水平。结果:按照体重指数配对的AHI<15/h与AHI>15/h的两组患者体重指数、年龄、腰臀比、颈围均无统计学差异。两组患者AHI水平有统计学差异(6.67±5.16/h vs 31.69±22.91/h,p=0.001);夜间最低血氧饱和度有统计学差异(88.07±6.69%vs 79.47±11.31%,p=-0.019)。AHI>15/h组患者胰岛素抵抗指数较AHI<15/h组患者升高并有统计学差异(1.47±0.58 vs 0.94±0.49,p=0.001)。AHI>15/h组患者空腹血清丙氨酸氨基转移酶水平较AHI<15/h组患者升高并有统计学差异(33.82±33.37 IU/L vs 20.06±13.64IU/L,p=0.040);乳酸脱氢酶水平升高并有统计学差异(165.83±37.50IU/Lvs144.93±21.28IU/L,p=0.047)。结论:随着OSAS严重程度增加,胰岛素抵抗程度增加,血清丙氨酸氨基转移酶及乳酸脱氢酶升高;提示OSAS可能通过间断缺氧途径及胰岛素抵抗途径导致肝功能损伤,引起非酒精性肝病。     

Novel insights into the consequences of obesity: a phenotype-wide Mendelian randomization study

Obesity is thought to significantly impact the quality of life. In this study, we sought to evaluate the health consequences of obesity on the risk of a broad spectrum of human diseases. The causal effects of exposing to obesity on health outcomes were inferred using Mendelian randomization (MR) analyses using a fixed effects inverse-variance weighted model. The instrumental variables were SNPs associated with obesity as measured by body mass index (BMI) reported by GIANT consortium. The spectrum of outcome consisted of the phenotypes from published GWAS and the UK Biobank. The MR-Egger intercept test was applied to estimate horizontal pleiotropic effects, along with Cochran’s Q test to assess heterogeneity among the causal effects of instrumental variables. Our MR results confirmed many putative disease risks due to obesity, such as diabetes, dyslipidemia, sleep disorder, gout, smoking behaviors, arthritis, myocardial infarction, and diabetes-related eye disease. The novel findings indicated that elevated red blood cell count was inferred as a mediator of BMI-induced type 2 diabetes in our bidirectional MR analysis. Intriguingly, the effects that higher BMI could decrease the risk of both skin and prostate cancers, reduce calorie intake, and increase the portion size warrant further studies. Our results shed light on a novel mechanism of the disease-causing roles of obesity.Subject terms: Lifestyle modification, Genetic predisposition to disease     

深圳市南山区MSM个体性网络大小对感染梅毒的影响

目的了解深圳市男男性行为者(men who have sex with men,MSM)个体性网络大小及其对感染梅毒的影响,为男男性行为者采取干预措施降低梅毒感染风险提供新的视角。方法 2014—2016年深圳市南山区慢性病防治院采用外展干预和男同彩虹门诊相结合的方式对男男性行为者开展匿名自填式问卷调查,收集男男性行为者基本人口学、行为学、性网络特征及梅毒知识知晓情况,开展HIV、梅毒检测,通过卡方检验、Logistic回归探索该人群性行为、性网络与梅毒的相关性。结果共调查429名男男性行为者,梅毒检测阳性率9.1%。平均年龄(27.2±5.8)岁;汉族占98.1%;文化程度大专以上占70%;户籍是外省的占一半以上;约50%在深圳居住时间在半年以内;梅毒预防知识知晓率较低,仅70.2%。个体性网络大小做单因素分析,年龄(P=0.009)、文化程度(P=0.012)、在深居住时间(P=0.002)、最近6月与男性发生肛交时安全套的使用情况(P=0.041)、梅毒知识知晓情况(P=0.047)均与最近6月个体性网络大小情况有统计学意义。梅毒感染单因素分析文化程度、最近6月个体性网络大小、最近6月与男性发生肛交时安全套使用情况与感染梅毒有统计学意义;多因素分析显示个体性网络大小为15人以上(adjusted OR=3.383,95%CI=0.997~23.030)是感染梅毒的危险因素;最近6月与男性发生肛交时每次使用安全套(adjusted OR=0.059,95%CI=0.011~0.305)是感染梅毒的保护因素。结论 MSM人群是梅毒的一个易感人群,感染梅毒会增加感染HIV的风险,预防HIV和梅毒需同步。性网络作为传播HIV/STI桥梁的同时也是预防的关键,通过性网络建立同伴健康教育干预方法是预防性病的重要措施。     

Reorganization of Lamellar Diblock Copolymer Poly(ε‐caprolactone)‐block‐poly(4‐vinylpyridine) in the Melting Temperature Range

The reorganization kinetics of the “original” lamellar diblock copolymer poly(ε‐caprolactone)‐block‐poly(4‐vinylpyridine) crystals formed at 260 K is studied in the melting region from 270 K (10 K below the onset of the melting peak of original crystals) to 310 K (the melting peak temperature) on the time scale starting from 10^?4 to 10² s by ultrafast differential scanning calorimetry. Different reorganization pathways are observed in this temperature range. Annealing at temperatures below 295 K leads to further stabilization of original crystals by secondary crystallization. At annealing temperatures higher than 295 K, crystals partially melt and the reorganization occurs via the melting–recrystallization. For even higher temperature, such as 310 K, the melting is completed within a few milliseconds and recrystallization starts from the nuclei formation. The sigmoidal recrystallization kinetics is analyzed by the Avrami equation. It is found that the copolymer experiences about one order of magnitude slower recrystallization rate and has higher melting peak temperatures of crystals formed after recrystallization than the homopolymer. The slower recrystallization kinetics in the copolymer is discussed from the viewpoint of the nanoscale spatial constraint and the intermediate state prior to the recrystallization.

     

靶向表达的逆转录病毒载体LN.ALBTRS.TK的构建

目的:构建出在肝细胞中具靶向表达潜能的逆转录 TK 基因载体。方法:用 p2335A-1中的一段2.0Kb的人白蛋白组织特异性转录调节序列(ALBTRS)取代 pSTK 中的 SV40启动子,所构建的载体命名为 LN.ALB-TRS.TK。结果:载体 LN.ALBTRS.TK 的结构中,含有 ALBTRS 启动子,具有在肝细胞中特异表达白蛋白的潜能,载体经酶切鉴定表明结构符合要求。结论:成功地构建出具靶向表达潜能的逆转录载体 LN.ALBTRS.TK。     

基于App的疾病自我评估在类风湿关节炎患者中的应用

目的探讨基于App的疾病自我评估对类风湿关节炎患者疾病活动度的影响。方法将风湿免疫科门诊就诊的类风湿关节炎患者119例纳入本研究,采用中心化随机分组并剔除失访者后最终纳入110例患者,其中实验组56例,对照组54例。实验组使用智能疾病管理系统(SSDM)进行疾病评估管理,对照组进行常规医疗随访。分别于基线期及24周后对患者进行病情评估,比较2组患者病情活动度。结果经24周治疗后,2组疾病活动度评分(DAS28)、关节疼痛数量、健康评估问卷(HAQ)评分、血沉改善程度比较,差异有统计学意义(P0.05),但2组简明生活质量量表(SF-36)评分比较,差异无统计学意义(P0.05)。结论基于App的疾病自我评估有利于类风湿关节炎患者的病情控制,有利于患者关节功能改善和生活质量的提高,对患者疾病结局有积极意义。     

Effect of SiO2–Al2O3 Glass Composite Coating on the Oxidation Behavior of Ti60 Alloy

A SiO₂–Al₂O₃ glass composite coating was prepared on Ti60 alloy via air spraying slurry and then a suitable baking process. It was composed of potassium silicate glass, alumina and quartz powders. The high temperature oxidation performance of the alloy with and without coating was evaluated in static air at both 800 °C and 900 °C. The results show that catastrophic oxidation occurs for Ti60 bare alloy. It had a mass gain of about 2 mg/cm² after oxidation at 800 °C and 17 mg/cm² at 900 °C for 100 h. On the contrary, the oxidation resistance of alloy coated with composite coating was much improved with the mass gain about 0.36 mg/cm² and 0.95 mg/cm² at 800 °C and at 900 °C, respectively. The microstructure evolution of the composite coating and the alloy was analyzed by scanning electron microscope and electron probe microanalyzer. The effect of the composite coating on the oxidation performance of the alloy is discussed especially in terms of oxygen diffusion and interfacial reaction.