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71.
72.
Maruyama Suguru Kawaguchi Yoshihiko Akaike Hidenori Shiraishi Kensuke Saito Ryo Shimizu Hiroki Furuya Shinji Hosomura Naohiro Amemiya Hidetake Kawaida Hiromichi Sudo Makoto Inoue Shingo Kono Hiroshi Ichikawa Daisuke 《World journal of surgery》2020,44(3):863-868
World Journal of Surgery - Gastric cancers located within the upper-third of the stomach (UGC), especially the esophagogastric junction GC (EGJGC), have distinct clinicopathological features due to... 相似文献
73.
Inoue Takahiro Ishihara Ryu Shibata Tomotaka Suzuki Kosuke Kitagawa Yuko Miyazaki Tatsuya Yamaji Taiki Nemoto Kenji Oyama Tsuneo Muto Manabu Takeuchi Hiroya Toh Yasushi Matsubara Hisahiro Mano Masayuki Kono Koji Kato Ken Yoshida Masahiro Kawakubo Hirofumi Booka Eisuke Yamatsuji Tomoki Kato Hiroyuki Ito Yoshinori Ishikawa Hitoshi Tsushima Takahiro Kawachi Hiroshi Oyama Takashi Kojima Takashi Kuribayashi Shiko Makino Tomoki Matsuda Satoru Doki Yuichiro 《Esophagus》2022,19(3):375-383
Esophagus - Endoscopic diagnosis of the invasion depth of superficial esophageal squamous cell carcinoma (ESCC) is an important determinant of the treatment strategy. The three endoscopic imaging... 相似文献
74.
Yoshizumi Takemura Tatsuya Yuba Kenji Kono Kunishige Hosogi Shuji Osugi Hiromi Kuwahara Kazuhiro Nagata Hidehiko Harada Ichiro Yokomura Yoshinobu Iwasaki 《Nihon Kokyūki Gakkai zasshi》2004,42(7):660-664
Thymic carcinoma is rarer than thymoma and carries a very poor prognosis. No standard treatment has yet been established. Chemotherapy and radiation therapy are usually given to patients in whom surgery is not indicated. We clinically review six patients with thymic carcinoma (three men and three women) who were admitted to our hospital from 1992 through 2002. Their ages ranged from 56 to 81 years (median 70.5). Four patients had chest pain, two were unable to swallow, and in one, who was asymptomatic, the tumor was detected by chance. Histologically, five cases were squamous cell carcinomas, and one was a lymphoepithelioma-like carcinoma. Four of the six patients had distant metastasis at diagnosis. Only one patient was able to undergo surgery, two received radiation therapy alone, and three were administered chemoradiation. The response to the chemoradiation was progression of the disease in two patients and no change in one. The response to radiation therapy was partial response in one patient and no change in one. In summary, chest pain is the most common symptom of thymic carcinoma. A symptomatic case suggests a poor prognosis resulting from the clinical behavior of thymic carcinoma, such as early distant metastasis or direct invasion of surrounding organs. Histologically, squamous cell carcinoma is the most common, and thymic carcinoma appears to be generally resistant to chemotherapy and radiotherapy. 相似文献
75.
The anatomy and pathology of the intrahepatic peribiliary glands were evaluated. In this study, we ultrastructuraly examined
the peribiliary glands of normal and hepatolithiatic livers using common and serial ultrathin section observations. It is
well known that these glands proliferate markedly in hepatolithiasis. These glands were composed of several acini surrounded
by thickened and multilayered basement membranes, and there were mesenchymal cells (the majority were fibroblasts) in the
periacinar fibrous connective tissue. Some cytoplasmic processes of acinar epithelial cells and mesenchymal cells in the periacinar
connective tissue were in close contact with each other within the thickened and multilayered basement membranes. Such cell-to-cell
interaction was most frequent in cases of hepatolithiasis, in which peribiliary glands proliferated markedly. In hepatolithiatic
livers, some unmyelinated nerve fibers or axonal button profiles were in close contact with periacinar mesenchymal cells and
also with cytoplasmic processes of glandular epithelial cells. Such contacts were rare in normal livers. These findings suggest
that such epithelial and mesenchymal cell interactions and innervations play a part in the normal regulation of peribiliary
glands and also in the proliferation of peribiliary galnds in hepatolithiasis. 相似文献
76.
T. Hayashi Dr. M. Okamoto Y. Yoshimasa G. Inoue K. Yamada S. Kono M. Shigemoto J. Suga H. Kuzuya K. Nakao 《Diabetologia》1996,39(5):515-522
Summary Phosphoinositide 3-kinase (PI3-kinase) plays a crucial role in insulin signal transduction. We studied the molecular mechanism of the insulin-induced activation of PI3-kinase in rat hepatoma Fao cells using an antibody against the 110-kDa catalytic subunit (p110) and two against the 85-kDa regulatory subunit (p85). PI3-kinase activity increased 1.6-fold in anti-p85 immunoprecipitates after insulin stimulation, whereas it did not increase when cell lysates were first immunoprecipitated with anti-phosphotyrosine or anti-insulin receptor substrate-1 (IRS-1), then with anti-p85, suggesting that the PI3-kinase which associates with tyrosyl phosphoproteins including IRS-1 is responsible for the increase in kinase activity. The activated PI3-kinase molecules constituted 4–6% of the total PI3-kinase, and their specific activity was 11–14 times higher than that of the basal state. Anti-p110 recognized the catalytically active form of p110, and immunoprecipitated p110 only after exposure to insulin. Hence, the epitope of anti-p110, P200-C215, seems to be included in the portion of p110, the conformation of which is changed by insulin stimulation. We conclude that, in response to insulin stimulation, only a small fraction of p85 in the PI3-kinase pool associates with tyrosyl phosphoproteins including IRS-1, and that the specific activity of p110 is increased presumably through a conformational change including the P200-C215 region.Abbreviations PI3-kinase
Phosphoinositide 3-kinase
- p85
85-kDa subunit of PI3-kinase
- p110
110-kDa subunit of PI3-kinase
- IRS-1
insulin receptor substrate-1
- SH2
src homology 2
- SH3
src homology 3
- BCR
breakpoint cluster region
- PMSF
phenylmethylsulphonyl fluoride
- HEPES
4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid
- PIP
phosphatidylinositol phosphate
- TLC
thin layer chromatography
- IP (in figures)
immunoprecipitation with the indicated antibody
- TBS
Tris-buffered saline 相似文献
77.
Transthyretin (prealbumin) in the pancreas and sera of newly diagnosed type I (insulin-dependent) diabetic patients. 总被引:1,自引:0,他引:1
N Itoh T Hanafusa J Miyagawa S Tamura M Inada S Kawata N Kono S Tarui 《The Journal of clinical endocrinology and metabolism》1992,74(6):1372-1377
We investigated transthyretin (TTR) in the pancreases and sera of 10 newly diagnosed type I diabetic patients by immunohistochemistry and nephelometry. In the type I diabetic pancreases, glucagon-positive A-cells showed strong immunoreactivity for TTR, the intensity and distribution pattern of which corresponded to those in normal subjects. Morphometric analysis revealed that the amount of strongly TTR-positive A-cells was not significantly different from that in normal subjects. On the contrary, insulin-positive B-cells, which normally show uneven and weak TTR immunoreactivity, decreased in number, and only a few residual B-cells showed faint immunoreactivity. Neither somatostatin cells nor pancreatic polypeptide cells were positive for TTR. The serum TTR concentration showed a significant decrease in type I diabetic patients compared with that in normal subjects (P less than 0.005). These data suggest that the synthesis or storage of TTR in A-cells is not affected, but that in B-cells is impaired in type I diabetes. The decrease in serum TTR might be one of the features of metabolic disorders in type I diabetes. 相似文献
78.
Tohru Murayama M.D. Shion Imoto M.D. Mitsuhiro Ito M.D. Keiko Matsushita M.D. Sachiko Matozaki M.D. Toshitaro Nakagawa M.D. Yoshihiro Kanbara M.D. Norio Kono M.D. Terumasa Sashikata M.D. 《The American journal of gastroenterology》1992,87(10):1503-1504
We report a case of fibromatosis, soft tissue tumors that are benign histologically, but exhibit behavior intermediate between benign and malignant diseases. Mesenteric fibromatosis grows vigorously without a general inflammatory reaction, and its symptoms are the result of obstruction and/or compression of the intestine. However, in our case, the general inflammatory reactions of fever and C-reactive protein (CRP) elevation was present at an early stage. Because this inflammatory reaction disappeared after surgical resection, it may have been induced by some inflammatory factors produced in the tumor, such as those produced in inflammatory fibrous histiocytoma. We found that computed tomography was useful in the detection of the cause of fever of unknown origin, and suggest that it should be recommended in cases of long-lasting unexplainable fever. 相似文献
79.
Structural origins of constitutive activation in rhodopsin: Role of the K296/E113 salt bridge 下载免费PDF全文
Kim JM Altenbach C Kono M Oprian DD Hubbell WL Khorana HG 《Proceedings of the National Academy of Sciences of the United States of America》2004,101(34):12508-12513
The intramolecular interactions that stabilize the inactive conformation of rhodopsin are of primary importance in elucidating the mechanism of activation of this and other G protein-coupled receptors. In the present study, site-directed spin labeling is used to explore the role of a buried salt bridge between the protonated Schiff base at K296 in TM7 and its counterion at E113 in TM3. Spin-label sensors are placed at positions in the cytoplasmic surface of rhodopsin to monitor changes in the structure of the helix bundle caused by point mutations that disrupt the salt bridge. The single point mutations E113Q, G90D, and A292E, which were previously reported to cause constitutive activation of the apoprotein opsin, are found to cause profound movements of both TM3 and TM6 in the dark state, the latter of which is similar to that caused by light activation. The mutant M257Y, which constitutively activates opsin but does not disrupt the salt bridge, is shown to cause related but distinguishable structural changes. The double mutants E113Q/M257Y and G90D/M257Y produce strong activation of the receptor in the dark state. In the E113Q/M257Y mutant investigated with spin labeling, the movement of TM6 and other changes are exaggerated relative to either E113Q or M257Y alone. Collectively, the results provide structural evidence that the salt bridge is a key constraint maintaining the resting state of the receptor, and that the disruption of the salt bridge is the cause, rather than a consequence, of the TM6 motion that occurs upon activation. 相似文献
80.