羊水过少66例临床分析

目的　分析在妊娠晚期羊水过少的早期诊断和终止妊娠方式。　方法　对海南省农垦总局医院 1990年月1日～ 2 0 0 3年 3月 66例羊水过少孕妇进行回顾性分析。　结果　妊娠 40周后羊水过少发生率及羊水污染发生率较妊娠 40周前明显增高 (P <0 0 0 1,P <0 0 5 ) ,分娩方式及新生儿预后差异无显著性 (P >0 0 5 )。　结论　妊娠达 40周后如无产兆应进行B超监测 ,及早诊断及处理羊水过少者 ,如无胎儿窘迫 ,可以阴道试产 ,必要时手术以提高围产儿出生质量。     

Involvement of IL-1beta in acute stress-induced worsening of cerebral ischaemia in rats.

Stress is known to be one of the risk factors of stroke. Most of the knowledge on the effects of stress on cerebrovascular disease in humans is restricted to catecholamines and glucocorticoids effects on blood pressure and/or development of atherosclerosis. However, few experimental studies have examined the possible mechanisms by which stress may affect stroke outcome. We have used an acute stress protocol consisting of the exposure of male Fischer rats to an acute, single exposure immobilisation protocol (6 h) prior to permanent middle cerebral artery occlusion (MCAO), and we have found that stress worsens behavioural and neurological outcomes and increased infarct size after MCAO. The possible regulatory role of the TNFalpha and IL-1beta was studied by looking at the release of these cytokines in brain. The results of the present study showed an increase in IL-1beta release in cerebral cortex after exposure to acute stress. Brain levels of IL-1beta are also higher in previously stressed MCAO rats than in MCAO animals without stress. Pharmacological blockade of IL-1beta with an antibody anti-IL-1beta led to a decrease in the infarct size as well as in neurological and behavioural deficits after MCAO. In summary, our results indicate that IL-1beta, but not TNFalpha, accounts at least partly for the worsening of MCAO consequences in brain of rats exposed to acute stress.     

Onychopathy in a Patient with Fetal Hydantoin Syndrome

Abstract: Hydantoin is an anticonvulsant drug with several side effects. A teralogenic potential has been suggested. The fetal hydantoin syndrome is an entity that consists of a broad range of morphologic and developmental disorders in children born of epileptic mothers exposed to hydantoin during pregnancy. We treated a girl in whom onychopathy was a monosymptomatic or mild form of this syndrome.     

护生自身练习护理操作的伦理问题及对策

自身练习护理操作就是以护生作为练习对象,相互间在各自身上完成注射、输液等操作练习项目,以达到教学要求[1]。在基础护理的教学中,自身练习对于调动护生的参与感,增加真实感,满足成就感方面起着十分重要的作用。但是,也必须看到在积极效果背后的种种矛盾以及由这些矛盾可能导     

Conners教师评定量表的中国城市常模和信度研究

目的　建立Conners教师评定量表 (TRS)的中国城市常模 ,并对其进行信度检验。方法　在全国 14个城市对 35 76名儿童年进行Conners教师量表的评定 ,对其中 4 0名儿童间隔二周进行重测。结果　TRS各条目分与量表总评分之间的相关系数在 0 2 6 5～ 0 6 89;总问卷Cronbachα系数为 0 94 1,Spearman brown分半信度为 0 896 ,间隔二周量表总分的重测相关系数为 0 5 4 4 ,4个因子的重测相关系数在 0 391～ 0 6 71(P <0 0 1)。结论　Conners教师评定量表的中国城市常模符合我国国情 ,具有良好的信度 ,值得推广和应用。     

Preparation and identification of monoclonal antibodies against Penicillium marneffei]

OBJECTIVE: To prepare and identify monoclonal antibodies (mAbs) against Penicillium marneffei. METHODS: Recombinant mannoprotein1 (MP1) of Penicillium marneffei was used to immunize BALB/c mice, and anti-MP1 mAbs were obtained by means of hybridoma. Screening and identification of the mAbs were subsequently performed with indirect enzyme-linked immunosorbent assay and Western blotting, respectively. RESULTS: Four hybridomas producing antibodies against Penicillium marneffei were obtained, and the IgG isotypes of the 4 mAbs were identified as IgG1 with affinity constants (K) of 8.2x10(-9), 4.7x10(-9), 6.5x10(-9) and 2.7x10(-9), respectively. Western blotting demonstrated specific recognition of Aspergillus fumigatus MP1 by the obtained mAbs. CONCLUSION: The 4 hybridomas producing anti-MP1 mAbs with high specificity and affinity can be of significant value in the diagnosis of Penicilliosis marneffei infections.