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81.
Shobha Malviya Frederick A. Burrows Albert E. Johnston Lee N. Benson 《Journal canadien d'anesthésie》1989,36(3):320-324
Anaesthetic and sedation techniques, complications and outcomes were reviewed in 176 children undergoing 184 interventional
cardiologic procedures. Techniques included sedation only, and ketamine, inhalational or narcotic anaesthesia. Ketamine infusion
was the technique most frequently used. Ketamine was associated with a higher incidence of respiratory complications (P <
0.05) than the other techniques. The higher incidence of hypercarbia (15.6 per cent), which did not affect outcome, may be
attributable to the use of supplemental sedatives. The incidence of upper airway obstruction (7.8 per cent) was similar to
that of previous studies. Vascular compromise resulted from the procedure in 33 patients, necessitating surgical correction
in 16. Cardiac perforation occurred in four cases, causing one death. Pulmonary valve stenosis was most amenable to balloon
dilatation and aortic valve stenosis least amenable. Ketamine was the anaesthetic agent preferred by cardiologists for use
in the catheterisation suite when general anaesthesia was required. Vigilant monitoring by anaesthetic staff is necessary
during the procedure, and avoidance of concomitant narcotics is recommended if a ketamine technique with spontaneous ventilation
is used.
Les techniques anesthésiques et de sédation ainsi que les complications et les issues ont été revues chez 176 enfants subissant
184 procedures cardiaques. Les techniques ont inctu soil la sédation seulement, soit l’anesthésie à la kétamine, aux agents
d’inhalation ou aux narcotiques. La perfusion de kétamine était la technique la plus fréquemment utilisée. La ketamine était
associée à une plus grande incidence de complication respiratoire (P < 0.05) comparativement aux autres techniques. La plus
grande incidence d’hypercarbie (15.6 pour cent), n’ayant pas affecté l’issue, pourrait être attribuée à l’utilisation additionnelle
de sédatifs. L’incidence d’obstruction des voies aériennes supérieures (7.8 pour cent) était similaire aux études préalables.
Un problème vasculaire suite à la procédure fut observé chez 33 patients dont 16 ont requis une correction chirurgicale. Une
perforation cardiaque est survenue dans quatre cas provoquant le décès d’un seul patient. La sténose de la valve pulmonaire
était la procédure la plus susceptible d’être dilatée et la sténose de la valve aortique la moins susceptible. La kétamine
était l’agent anesthésique préféré par les cardiologistes lors des cathétérisations quand une anesthésie générate était requise.
Une surveillance vigilante par une équipe anesthésique fut nécessaire durant la procedure. Il faut aussi éviter l’administration
de narcotiques si la kétamine est administrée en respiration spontanée.
Presented in part at the Canadian Anaesthetists’ Society annual meeting in Halifax, June 1988. 相似文献
Presented in part at the Canadian Anaesthetists’ Society annual meeting in Halifax, June 1988. 相似文献
82.
Prenatal detection of Turner''s syndrome in conjunction with trisomy 20 mosaicism (45,X/46, X, +0). 下载免费PDF全文
A case of Turner's syndrome, detected antenatally and complicated by the finding of trisomy 20 mosaicism in 50% of cells from each of two amniotic fluid cultures, is described. Cultures from seven fetal tissues in the subsequent abortus showed a predominance of 45,X cells, but nevertheless suggested the existence of a very low level of trisomy 20 mosaicism in three fetal tissues. The diagnostic dilemma in interpreting trisomy 20 mosaicism is discussed. 相似文献
83.
Verschuren MC; Blom B; Bogers AJ; Spits H; van Dongen JJ 《International immunology》1998,10(12):1873-1880
Recombination of deltaRec to psiJalpha will delete the TCR delta gene,
which is thought to play an important role in the bifurcation of the TCR
alphabeta versus TCR gammadelta differentiation lineages. We recently
detected a DNA-binding protein in human thymocytes, the so- called PJA-BP,
which recognizes the psiJalpha gene segment and might be one of the factors
involved in the regulation of preferential deltaRec- psiJalpha
rearrangements. We now investigate PJA-BP expression and its correlation
with TCR delta gene deletion in thymocytes. Our electrophoretic mobility
shift assay experiments showed that the PJA-BP is evolutionary conserved in
human, murine and simian thymocytes. Using a large series of human
hematopoietic malignancies (n = 30), we conclude that PJA-BP expression is
thymocyte specific and seems to be restricted to thymocytes committed to
the TCR alphabeta lineage. Analysis of seven well-defined human thymocyte
subpopulations showed that preferential deltaRec-psiJalpha rearrangements
as well as PJA-BP expression can be detected from the immature
CD34-/CD1+/CD3- /CD4+/CD8alpha+beta- thymocyte differentiation stage
onwards. These experiments indicate that expression of PJA-BP in human
thymocytes starts simultaneously with preferential deltaRec-psiJalpha
rearrangements, which supports our hypothesis that PJA-BP is one of the
factors involved in the preferential recombination of deltaRec to
psiJalpha.
相似文献
84.
P. J. Vaitkus Dr K. W. Johnston R. L. Bondar F. Stein P. A. J. Bascom Ly Mo M. Kassam L. C. Chadwick J. Mehi R. S. C. Cobbold 《Medical & biological engineering & computing》1990,28(4):306-311
During space flights, several clinical syndromes may be the result of changes in cerebral circulation. The purpose of the
paper is to describe the development and initial evaluation of a system for recording, processing and displaying transcranial
Doppler ultrasound (TCD) waveforms from the middle cerebral artery (MCA) in microgravity. Volunteers were repeatedly subjected
to 15–20 s intervals of microgravity (‘near zero gravity’) during flights on the KC-135 military aircraft. Continuous TCD
recordings from the MCA were stored on magnetic tape. The paper describes the system that was developed to digitise the Doppler
ultrasound data and markers that corresponded to the various levels of microgravity, obtain the maximum and mean Doppler waveforms,
identify the waveforms and quantify them. The results demonstrate the feasibility of making TCD recordings in a microgravity
environment and illustrate excellent performance of the system and its ease of operation. Quantitative waveform analysis of
the recordings from the first subject studied in the supine position showed statistically significant changes in MCA velocity
waveforms during microgravity. 相似文献
85.
H. H. Johnston J. F. Acar G. Linzenmeier A. Visconti 《European journal of clinical microbiology & infectious diseases》1982,1(4):204-211
The MS-2 is an instrument for rapid automated testing of antimicrobial susceptibility. Its performance was evaluated by comparison with disc diffusion and MIC tests in a collaborative study in four European laboratories. Tests on independently isolated organisms showed the MS-2 to be in essential agreement with conventional methods in 94.8% of tests. A further series of 170 welldefined pathogens for which a reference antibiogram was available were tested by MS-2 and the manual methods in all of the participating laboratories. MS-2 results were in full accord in 90 % of tests and in essential agreement in 94 %. MS-2 results compared at least as well with the reference values as did either of the manual methods. Initial problems of false susceptibility results with erythromycin and penicillin were resolved by (1) the addition of small amounts of erythromycin which acted as an inducer and (2) by the use of a low content penicillin disc. MS-2 was found to be reliable and needed no attention following loading of the test cuvette cartridges. A print-out of the results was available 2–5 h after inception of the test. 相似文献
86.
Sanjana VM; Johnston PA; Robertson CR; Jamison RL 《The American journal of physiology》1976,231(2):313-318
87.
WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae 总被引:13,自引:1,他引:13 下载免费PDF全文
The growth requirements of 135 clinical isolates of Neisseria gonorrhoeae and six American Type Culture Collection reference strains were examined by using a simple chemically defined medium known as Wong-Shockley-Johnston medium, WSJM. The simple liquid medium supported growth of gonococci from an inoculum of 2 X 10(6) colony-forming units ml-1 and yielded 10(10) colony-forming units ml-1 in 10 h in the absence of CO2. Scale-up experiments with the complete medium yielded 5 to 10 g, wet weight, of cells per liter. The complete medium was stable upon storage at 5 degrees C and after lyophilization. 相似文献
88.
Sigmundsdóttir H Johnston A Gudjónsson JE Valdimarsson H 《Clinical immunology (Orlando, Fla.)》2004,111(1):119-125
At both cutaneous and mucosal sites, interleukin (IL)-10, IL-12 and transforming growth factor (TGF)-beta are important regulators of chronic inflammatory disease, where cutaneous lymphocyte-associated antigen (CLA) and alphaE integrin (CD103) may be expressed. Stimulation with streptococcal pyrogenic exotoxin C (SpeC) increased the expression of CD103 by CD8+ but not CD4+ T cells. While adding IL-12 augmented the expression of CLA, superantigen-induced expression of CD103 was markedly suppressed by IL-12, which could be reversed by TGF-beta. Antibodies against TGF-beta inhibited, and a combination of anti-TGF-beta and IL-12 completely abrogated the induced CD103 expression. IL-10 strongly decreased the frequency of CLA+ and although not increasing the frequency of CD103+CD8+ T cells, the amount of CD103 expressed per cell was markedly increased. Thus, the expression of CLA and CD103 may be antagonistically regulated by IL-10 and IL-12 and the balance between these cytokines could influence the T cell migration of inflammatory cells into epithelial tissues. 相似文献
89.
Janine L. Johnston James A. Sharpe 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1994,99(2):302-308
The gain (ratio of eye velocity to head velocity) of the initial horizontal vestibulo-ocular reflex (VOR) was calculated in 12 normal subjects over 350 ms during impulsive, unpredictable whole body rotation under three conditions: (1) darkness; (2) visual enhancement of the VOR, while the subjects fixated a stationary target; and (3) visual cancellation of the reflex, while subjects fixated a target that rotated with the head. The gain of the initial 80 ms of compensatory eye movement increased significantly during visual fixation in 5 subjects and decreased during attempted VOR cancellation in 3 subjects, when compared with VOR gain in darkness. Compensatory vestibular smooth eye movements were slowed, becoming curved at the onset of VOR cancellation, at mean latencies ranging from 78 to 149 ms in individual subjects (group mean 128 ms). At about 190 ms, quick phases moved the eyes in the same direction as head and target motion. The subsequent vestibular eye movements were about 50% slower than the initial smooth eye movements, indicating more effective cancellation. Visual enhancement of the VOR can occur prior to the onset of pursuit, providing evidence that fixation and smooth pursuit are distinct ocular motor systems. Visual cancellation of the VOR also begins prior to smooth pursuit initiation and becomes more effective after the latency of smooth pursuit. 相似文献
90.
Del-Favero J; Krols L; Michalik A; Theuns J; Lofgren A; Goossens D; Wehnert A; Van den Bossche D; Van Zand K; Backhovens H; van Regenmorter N; Martin JJ; Van Broeckhoven C 《Human molecular genetics》1998,7(2):177-186
Autosomal dominant cerebellar ataxia with retinal degeneration (ADCAII) was
previously mapped by linkage analysis studies to chromosome 3p12- p21.1
(SCA7). Positional cloning efforts have recently identified a novel gene,
SCA7 , containing a translated CAG repeat, expanded in SCA7 patients. We
cloned the SCA7 gene from a yeast artificial chromosome (YAC) clone contig
spanning the SCA7 candidate region. Using a combination of genomic
sequencing and cosmid-based exon trapping, two expressed sequence tags were
identified. Sequencing of the corresponding cDNA clones and RT-PCR analysis
identified the full- length SCA7 cDNA. Together, our sequence data defined
the intron/exon boundaries of the first two coding exons of the SCA7 gene,
with the first exon containing the expanded CAG repeat. Further, sequence
comparison with the published SCA7 cDNA identified one additional putative
exon in the 5'-UTR region of the SCA7 gene. The SCA7 gene was mapped on the
YAC contig in the 2.5 cM interval between D3S1600 and D3S1287. In one
extended Belgian SCA7 pedigree the expanded alleles ranged from 38 to at
least 55 repeats with allele lengths being inversely correlated with onset
age of ADCAII symptoms. The SCA7 repeats increased in length in successive
generations. Normal alleles had from four to 18 repeats, with 10 repeats
being the most common allele.
相似文献