与甲壳素缝线体外复合培养大鼠成肌细胞株L6细胞的形态学

目的：探讨甲壳素作为骨骼肌组织工程支架材料的可行性。 方法：实验于2002-06／12在南方医科大学应用解剖学研究所完成。大鼠成肌细胞株L6与甲壳素医用缝合线体外复合培养。倒置相差显微镜下观察细胞的形态和排列情况。并分别在体外复合培养的第1，2，3，4，5，6天，取出细胞支架复合体，扫描电镜观察成肌细胞的形态和排列情况。 结果：①扫描电子显微镜观察显示，甲壳素医用可吸收缝线的表面比较粗糙。②倒置相差显微镜下可见大鼠L6细胞与甲壳素制备的医用可吸收缝合线间黏附良好，细胞沿缝线排列呈现串珠样，并可以见到细胞聚集现象。③扫描电子显微镜观察，接种24h后，细胞主要为圆球形，随后细胞迁移、伸展、并见突起；第3天，成肌细胞主要为梭形，并见融合趋势；4d后，细胞沿材料纵轴排列并相互融合，并见肌小管样结构形成及细胞外基质分泌。 结论：甲壳素具有作为支架以构建组织工程化骨骼肌的潜能，支架的平行排列有助于工程化骨骼肌纤维良好方向性的形成。     

Clinical oncology—A new era

Rapid growth in biomedical research coupled with dramatic advancement in biotechnology has significantly improved our understanding of the molecular basis involving cancer development and progression. This improvement has led to the discovery of new molecular markers for cancer diagnosis and prognosis as well as new molecular targets for cancer treatment and intervention. Continuous emergence of some new developing area in molecular profiling, new therapeutic agents, tissue microenvironment and systems biology have made significant progress in clinical oncology. Clinical research and investigation that focus on these new developments have begun to show exciting results that indicate future promises in improving patient management and survival.     

急性脑血管病病人应激状态的临床特点及护理

[目的 ]分析急性脑血管病(ACVD)应激状态的临床特点 ,探讨防治策略及护理对策。 [方法 ]回顾分析 3 62例ACVD病人的临床资料。[结果 ]ACVD病人应激反应的发生率为 47.5 1% ,病死率为 11.88%。 [结论 ]早期明确诊断与治疗 ,并采取措施积极调控机体应激状态 ,加强护理 ,有助于控制病情和促进康复 ,降低病死率。     

The Effect of Dry Cleared Extract from Licorice Roots on the Learning of Ovariectomized Rats

The influence of chronic administration of a dry cleared extract of Glycyrrhiza glabra (DCEG) containing flavonoids on the behavioral status and learning of intact and ovariectomized adult female rats has been studied. The DCEG preparation was administered in low (0.5 mg/kg, i.p.) and high (5.0 mg/kg, i.p.) daily doses over a period of 14 days and the results were evaluated on the passive avoidance situation model and in the open field test. It was established that the chronic administration of DCEG in both low and high doses produced correction of the passive avoidance performance in ovariectomized female rats. The administration of DCEG in a low dose also positively influenced the structure of purposeful behavior of females with deficiency of estrogens. __________ Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 39, No. 8, pp. 27 – 29, August, 2005.     

苏励教授治疗强直性脊柱炎经验介绍

苏励教授认为，脊柱在人体的位置处于皮里膜外，半表半里。强直性脊柱炎病在脊柱，故痰瘀之邪也盘踞在半表半里。治疗宜内清外透结合，在清里基础上外透，使邪有出路，从而清除盘踞在半表半里之邪实。并结合外治法以增加疗效。     

丙泊酚用于心脏电复律术前麻醉的效果观察与护理

目的:探讨丙泊酚用于心脏电复律术前麻醉的效果及护理措施。方法:将24例行心脏电复律术的患者随机分为实验组和对照组各12例,实验组给予丙泊酚2.5mg/kg术前麻醉,对照组给予地西泮0.3~0.5mg/kg术前麻醉,对两组患者麻醉起效时间、苏醒时间和术后不良反应进行比较。结果:实验组麻醉效果优于对照组,术后不良反应明显少于对照组,经统计学处理差异有显著性(P<0.01)。结论:丙泊酚用于心脏电复律术前麻醉,不良反应少,效果好,操作时间短,适合临床推广应用。     

Characterization of bortezomib-adapted I-45 mesothelioma cells

Background  

Bortezomib, a proteasome-specific inhibitor, has emerged as a promising cancer therapeutic agent. However, development of resistance to bortezomib may pose a challenge to effective anticancer therapy. Therefore, characterization of cellular mechanisms involved in bortezomib resistance and development of effective strategies to overcome this resistance represent important steps in the advancement of bortezomib-mediated cancer therapy.     

卫生保健课程教学误区之我见

卫生保健课程是以贯彻＂预防为主＂的方针、实现＂健康为人人,人人为健康＂的一门重要学科。笔者根据数年的教学实践,结合学生的课堂调查结果,总结卫生保健课程教学中常见的误区,以期同行在教学中避免走进误区,更好地为教学服务。