姜黄素对人SPC-A549细胞生长抑制的影响

目的　研究姜黄素对人肺腺癌细胞SPC -A5 4 9的生长抑制作用。方法　采用细胞培养 ,倒置相差显微镜下观察细胞形态变化 ;MTT法检测姜黄素对肺腺癌细胞的抑制作用。结果　①姜黄素作用癌细胞后 ,光镜下可见细胞脱壁 ,胞体缩小 ,胞核固缩。② 5、10、2 0和 4 0mg/L姜黄素均能抑制人肺腺癌细胞SPC -A5 4 9的生长 ,4 0mg/L姜黄素作用 12h细胞存活率达2 8.5 %。结论　姜黄素能有效的抑制人肺腺癌细胞SPC -A5 4 9的生长 ,且对人SPC -A5 4 9细胞存活率具有浓度和时间依赖性     

行为干预对高脂血症病人血脂的影响

我国人群血脂平均水平低于发达国家,但其升高幅度却很快.从20世纪80年代初至20世纪90年代末血清总胆固醇(Tc)高,男性患病由17%连续上升至33%,女性患病率由9%连续上升至32%[1].因此,及时检出,采取有效的应对措施,预防和控制血脂升高成为护理工作的主要内容.现将我院2006年6月门诊高脂血症病人实施行为干预对血脂水平影响的措施介绍如下.     

骨髓基质干细胞心肌化诱导过程中转录因子NKx 2.5的表达

目的：研究5-氮杂胞嘧啶(5-azacytidine，5-aza)和二甲亚砜(dimethyl sulfoxide．DMSO)诱导人骨髓基质细胞(human bone marrow stromal cells，hBMSC)心肌分化过程中心肌特异转录因子NKx2.5表达的变化。方法：取传至第8代hM-SC，以3μmol/L的5-aza和DMSO诱导24h。1周后重复诱导1次。在诱导后1、2和3周3个时间点以半定量RT-PCR方法检测细胞心肌特异转录因子NKx2.5的表达变化。结果：hMSC经5-aza诱导后，约1周左右细胞明显变大，2周时细胞走向趋于一致．邻近细胞间连接较前紧密，并形成肌节样结构。RT-PCR的结果显示，5-aza可诱导心肌特异转录因子NKx2.5，并一直持续表达3周。在DMSO诱导hBMSC后1周，开始可检测到细胞发生转录水平变化NKx2.5表达，并均可持续表达至诱导后3周；NKx2.5表达量有随诱导后时间延长而增加的趋势。结论：5-aza和DMSO在体外可诱导心肌特异转录因子NKx2.5的表达，从而启动hMSC心肌化的过程。     

3例腕部头静脉输液致桡神经浅支卡压综合征原因探讨

Dellon[1]1986年首次报道了桡神经浅支卡压综合征,认为该病在临床上并不少见,是腕部疼痛的重要原因,常见病因为腕部反复活动或局部外伤、扭伤致长期频繁牵拉、摩擦桡神经浅支,造成该神经行程中卡压、粘连,病人出现腕部及手背桡侧半麻痛不适,以致影响患手用力活动.     

Nesfatin-1 Decreases Excitability of Dopaminergic Neurons in the Substantia Nigra

Nesfatin-1, a newly discovered satiety molecule which reduces feeding behavior, has been recognized as a unique regulatory neuropeptide with its multiple roles, both central and peripheral. However, whether it had neuronal modulation effect on dopaminergic neurons is largely unknown. In the present study, using whole-cell patch clamp under current-clamp mode, we investigate the effects of nesfatin-1 on the electrical activity of rat nigral dopaminergic neurons. Nesfatin-1 could produce a resting membrane potential hyperpolarization on the majority of dopaminergic neurons tested. The spike frequency decreased by 23.13?±?5.93 and 43.20?±?5.56 % in 5-nM and 10-nM nesfatin-1 groups, respectively. These effects persisted in the presence of ionotropic glutamate and GABA receptor antagonists. Our study suggests that nesfatin-1 postsynaptically inhibits the electrical activity of nigral dopaminergic neurons.     

Involvement of Protease-Activated Receptor 2 in Nociceptive Behavior in a Rat Model of Bone Cancer

Treatment for bone cancer pain remains a clinical challenge due to a poor understanding of the underlying mechanisms. Protease-activated receptor 2 (PAR2), a receptor for inflammatory proteases, has been implicated in nociceptive signaling under both normal and pathologic pain states. However, little is known of the role of PAR2 in cancer-induced bone pain. Here we investigated the potential role of PAR2 in a rat model of bone cancer pain. The model of bone cancer pain was induced by inoculating Walker 256 into the tibia bone cavity of rats and verified by X-ray imaging, pathology, and behavior assessments. The rats with bone cancer exhibited marked mechanical allodynia, thermal hyperalgesia, and signs of spontaneous nocifensive behavior. Subcutaneous administration of the PAR2 antagonist FSLLRY-NH2 almost completely abolished mechanical allodynia and thermal hyperalgesia but had no effects on spontaneous pain behavior in the rats with bone cancer. Immunohistochemical study revealed that the expression of PAR2 was significantly increased in large- and medium-sized dorsal root ganglia (DRG) neurons but not in small-sized neurons after Walker 256 inoculation. These results suggest that the increased expression of PAR2 in the DRG may contribute to the development of mechanical allodynia and thermal hyperalgesia associated with bone cancer rats. PAR2 might become a novel target for the treatment of pain in patients with bone cancer.     

Double deletion of orexigenic neuropeptide Y and dynorphin results in paradoxical obesity in mice

Objective

Orexigenic neuropeptide Y (NPY) and dynorphin (DYN) regulate energy homeostasis. Single NPY or dynorphin deletion reduces food intake or increases fat loss. Future developments of obesity therapeutics involve targeting multiple pathways. We hypothesised that NPY and dynorphin regulate energy homeostasis independently, thus double NPY and dynorphin ablation would result in greater weight and/or fat loss than the absence of NPY or dynorphin alone.

Design and methods

We generated single and double NPY and dynorphin knockout mice (NPYΔ, DYNΔ, NPYDYNΔ) and compared body weight, adiposity, feeding behaviour, glucose homeostasis and brown adipose tissue uncoupling protein-1 (UCP-1) expression to wildtype counterparts.

Results

Body weight and adiposity were significantly increased in NPYDYNΔ, but not in NPYΔ or DYNΔ. This was not due to increased food intake or altered UCP-1 expression, which were not significantly altered in double knockouts. NPYDYNΔ mice demonstrated increased body weight loss after a 24-h fast, with no effect on serum glucose levels after glucose injection.

Conclusions

Contrary to the predicted phenotype delineated from single knockouts, double NPY and dynorphin deletion resulted in heavier mice, with increased adiposity, despite no significant changes in food intake or UCP-1 activity. This indicates that combining long-term opioid antagonism with blockade of NPY-ergic systems may not produce anti-obesity effects.     

Categorical or Dimensional: How Do Attachment Measures Inform Clinicians in Couple Therapy?

Self-report measures of adult romantic attachment have been widely used in research but their application in clinical practice has not been adequately examined. One important issue is the selection of a practical and reliable attachment measure that therapists can rely on in couple therapy. In the present study, the three-category Attachment Style Prototype (Hazan & Shaver, 1987) representing the original classic conceptualization of attachment, and the Experiences in Close Relationships (Brennan, Clark, & Shaver, 1998), a more recent scale with two dimensions representing a new conceptualization, were compared. Experiences in Close Relationships data were also used to establish four clusters based on the scores of the two dimensions. The Experiences in Close Relationships and Attachment Style Prototype categories were related in meaningful ways; however, Attachment Style Prototype was less effective in detecting a group of insecurely attached individuals who tended to self-identify as securely attached. Experiences in Close Relationships clearly shows an advantage over Attachment Style Prototype in clinical application, and therefore was recommended. Examples of the clinical utilization of Experiences in Close Relationships in couple therapy were provided using Experiences in Close Relationships scores from couples seeking therapy.