四君子汤改善抗生素脱污染小鼠肠道菌群失调的研究

目的：研究中药四君子汤对肠道菌群失调小鼠的调节作用。方法：小鼠经盐酸林可霉素灌肠造成菌群失调模型。将小鼠分自然恢复组。丽珠肠乐组，四君子汤组，治疗后，进行组织病理学和肠道菌群检测。结果：四君子汤组中肠道菌群最大幅度增加，与自然恢复组相比差异显。肠道粘膜损伤修复比自然恢复组好。结论：四君子汤能够促进肠粘膜损伤修复。调节肠道菌群失调。是一种理想的微生态调节剂。     

费氏艾美尔球虫（E.ferrisi）某些生物学特性的研究

采用从自然感染球虫的小鼠粪便中分离的单卵囊接种小鼠获得成功，观察了此种球虫的卵囊形态，孢子化时间，潜隐期和寄生部位等，确定此球虫为费氏艾美尔球虫。为进行费氏艾美尔球虫病的流行病学调查和防治提供了依据。     

舒血宁治疗脑衰弱综合征的双盲对照研究

１４３例脑衰弱综合征患者随机分成舒血宁组和对照组，进行双盲的前瞻性研究。通过脑衰弱量表，打洞试验，连线实验的回查，结果表明：应用舒血宁治疗脑衰弱综合征，整体疗效明显优于对照组，脑衰弱量表的总评分显著优于对照组，而且对脑衰弱量表各个症状都有显著改善，易激惹，烦恼两项症状降分幅度较大，打洞、连线试验表明舒血宁治疗后患者的注意力、记忆力都有明显的改善，而且没有发现明显副作用。作者认为舒血宁治疗脑衰弱综合征有显著的作用，作为一种治疗脑血管疾患伴发精神症状和早期预防脑血管痴呆的新药是可行的。     

胃癌单抗检测活检粘膜组织833例结果分析

应用胃癌单抗ID1-2对833例内窥镜活检组织进行了免疫学荧光检查,胃癌阳性检出率为81.6％:胃溃疡为32.7％;肠化生性蒌缩性胃炎为40.0％;食管和结肠腺癌分别为60.0％和62.5％,与单纯性萎缩性胃炎和浅表性胃炎的阳性检出率相比有非常显著的差异(P<0.01)。表明ID1-2单抗可用于检测活检粘膜组织中的癌细胞以及具有癌细胞相关抗原的癌前病变组织,如果与内窥镜及常规病理学检查联合应用,将可进一步提高早癌的阳性检出率。     

Scrub typhus vaccine candidate Kp r56 induces humoral and cellular immune responses in cynomolgus monkeys

A truncated recombinant 56-kDa outer membrane protein of the Karp strain of Orientia tsutsugamushi (Kp r56) was evaluated in cynomolgus monkeys (Macaca fascicularis) for immunogenicity and safety as a vaccine candidate for the prevention of scrub typhus. This recombinant antigen induced strong humoral and cellular immune responses in two monkeys and was found to be well tolerated. Antigen-specific immunoglobulin M (IgM) and IgG were produced to almost maximal levels within 1 week of a single immunization. Peripheral blood mononuclear cells from vaccinated animals showed an induction of antigen-specific proliferation and gamma interferon production. The Kp r56 was not as efficient as infection with live organisms in preventing reinfection but was able to reduce the inflammation produced at the site of challenge. This report describes the results of the first systematic study of the immunogenicity of a recombinant scrub typhus vaccine candidate in a nonhuman primate model.     

中国江苏地区汉族人群肿瘤坏死因子β遗传多态性研究

目的　探讨中国江苏地区汉族人群肿瘤坏死因子 β遗传多态性分布。方法　收集江苏地区 16 8名无血缘关系健康个体的静脉血提取DNA ,应用聚合酶链反应限制性片段长度多态性 (PCR -RFLP) ,对TNFβ基因多态性进行分析。结果　TNFβ检测到三个等位基因 ,其基因型频率分别为TNFβ 1/ 1=2 1 4 % ,TNFβ 1/ 2 =4 7% ,TNFβ 2 / 2 =31 5 % ,基因型分布符合Hardy-Weinberg定律。统计分析显示 :江苏地区汉族人群TNFβ等位基因频率分布与欧美白种人均有显著性差异(P <0 0 5 )。结论　TNFβ等位基因频率分布具有种族差异     

磷酸化的细胞外信号调节蛋白激酶1/2在正常小鼠脑内的免疫细胞化学定位

细胞外信号调节蛋白激酶(ERK)1/2是重要的信号转导分子。现已知在正常动物的中枢神经系统内有其活化形式即磷酸化的ERK1/2(pERK1/2)分子的存在,但其在小鼠脑内的分布目前还没有全面的观察。本研究用免疫组织化学技术(ABC法)研究了pERK1/2样免疫反应阳性产物在脱臼处死的正常小鼠全脑内的分布。结果发现pERK1/2在正常小鼠脑内有广泛的表达,阳性产物主要存在于神经元,亦见于个别白质内的胶质细胞,脑膜及室管膜细胞也有表达。强阳性表达的核团主要有:岛皮质、视听皮质、边缘前皮质、扣带前皮质、海马垂直部、弓状核、蓝斑和小脑Purkinje细胞;中等阳性表达的核团主要有:感觉运动皮质、外侧隔区、内侧杏仁核、皮质杏仁核和外侧杏仁核、丘脑室旁核前部、视交叉上核、穹隆下器、终板血管器、前腹侧视前核和下丘脑背内侧核;弱阳性表达的核团主要有:视上核、下丘脑室旁核大细胞部、下丘脑后区、顶盖前区、室周灰质腹外侧柱、A5区、孤束核和延髓腹外侧网状结构等。本文结果观察到pERK1/2在正常小鼠脑内广泛存在,提示pERK1/2作为重要的信号转导分子,可能参与许多脑区在正常状态下的功能活动,揭示其分布特点为了解其在脑内的多样性功能提供了形态学依据。     

Analysis of glioma cell lines for amplification and overexpression of MDM2

Recently, amplification of the gene encoding a p53 binding protein, MDM2, was determined in 8% of the cases constituting a large series of glioblastomas. Here we have utilized Southern blot analysis to examine 30 cell lines established from such tumors, and our investigation has revealed large increases in MDM2 gene dosage in two cases, one of which showed coamplification of the CDK4 gene that resides in close proximity to MDM2 in chromosomal region 12q13–14. Northern analysis demonstrated overexpression of MDM2 mRNA in the two cell lines with gene amplification, and overexpression of MDM2 protein was evident in each of these by immunohistochemical and Western blot analysis. Analysis of TP53 cDNAs revealed normal TP53 sequences in the cell lines with MDM2 amplification; these results are consistent with those of previous studies suggesting that MDM2 amplification occurs only in tumors expressing wild-type p53. In total, these data suggest that MDM2 amplification in glioblastoma cell lines occurs at a frequency (6.7%) comparable to that determined in primary tumors; occurs in cell lines expressing wild-type p53; and can involve the coamplification of additional genes.