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71.
Piyush Jain Weizhen Liu Siyu Zhu Christine Yao-Yun Chang Jeff Melkonian Fulton E. Rockwell Duke Pauli Ying Sun Warren R. Zipfel N. Michele Holbrook Susan Jean Riha Michael A. Gore Abraham D. Stroock 《Proceedings of the National Academy of Sciences of the United States of America》2021,118(23)
Leaf water potential is a critical indicator of plant water status, integrating soil moisture status, plant physiology, and environmental conditions. There are few tools for measuring plant water status (water potential) in situ, presenting a critical barrier for developing appropriate phenotyping (measurement) methods for crop development and modeling efforts aimed at understanding water transport in plants. Here, we present the development of an in situ, minimally disruptive hydrogel nanoreporter (AquaDust) for measuring leaf water potential. The gel matrix responds to changes in water potential in its local environment by swelling; the distance between covalently linked dyes changes with the reconfiguration of the polymer, leading to changes in the emission spectrum via Förster Resonance Energy Transfer (FRET). Upon infiltration into leaves, the nanoparticles localize within the apoplastic space in the mesophyll; they do not enter the cytoplasm or the xylem. We characterize the physical basis for AquaDust’s response and demonstrate its function in intact maize (Zea mays L.) leaves as a reporter of leaf water potential. We use AquaDust to measure gradients of water potential along intact, actively transpiring leaves as a function of water status; the localized nature of the reporters allows us to define a hydraulic model that distinguishes resistances inside and outside the xylem. We also present field measurements with AquaDust through a full diurnal cycle to confirm the robustness of the technique and of our model. We conclude that AquaDust offers potential opportunities for high-throughput field measurements and spatially resolved studies of water relations within plant tissues.Plant life depends on water availability. In managing this demand, irrigated agriculture accounts for of all human water use (1). Physiologically, the process of transpiration () dominates this demand for water (Fig. 1A): Solar thermal radiation and the unsaturated relative humidity in the atmosphere drive evaporation from the wet internal surfaces of leaves; this water loss pulls water up through the plant’s vascular tissue (xylem) and out of the soil. This flow occurs along a gradient in the chemical potential of water, or water potential, [MPa] (2). Studies of water relations and stress physiology over the past decades have found that values of along the path of (the soil–plant–atmosphere continuum [SPAC]) correlate with plant growth, crop yield and quality, susceptibility to disease, and the balance between water loss due to and the uptake and assimilation of carbon dioxide (water-use efficiency) (3–5).Open in a separate windowFig. 1.AquaDust as an in situ reporter of water potential (). (A) Schematic representation of a maize plant undergoing transpiration () in a dynamic environment driven by solar thermal radiation () and photosynthetically active radiation (), wind speed (), temperature (), vapor pressure deficit (), and soil water potential (). Water flows through the plant (blue arrows) along a gradient in water potential (). Zones on the leaves infiltrated with AquaDust serve as reporters of the local leaf water potential, , via a short ( s), minimally invasive measurement of FRET efficiency () with a leaf clamp. (B) Schematic representations of infiltration of a suspension of AquaDust and of the distribution of AquaDust within the cross-section of a leaf. AquaDust passes through the stomata and localizes in the apoplastic spaces within the mesophyll; the particles are excluded from symplastic spaces and the vascular bundle. (C) Schematic diagrams showing mechanism of AquaDust response: The swollen, “wet” state when water potential in its local environment, (i.e., no stress condition), results in low FRET between donor (green circles) and acceptor (yellow circles) dye (Upper); and the shrunken, “dry” state when (i.e., stressed condition) results in high FRET between fluorophores, thereby altering the emission spectra (Lower). (D) Fluorescent dyes were chosen to minimize reabsorption of AquaDust emission from chlorophyll; comparison of representative fluorescent emission from AquaDust (donor peak at 520 nm and acceptor peak at 580 nm) with the absorption spectra of chlorophyll and autofluorescence of maize leaf.Due to the recognized importance of water potential in controlling plant function, plant scientists have spent considerable effort devising accurate and reliable methods to measure water potential of the soil, stem, and leaf (6). Of these, plant water potentials, and particularly leaf water potential (), represent valuable indicators of plant water status because they integrate both environmental conditions (e.g., soil water availability and evaporative demand) and plant physiological processes (e.g., root water uptake, xylem transport, and stomatal regulation) (7, 8). To date, techniques to measure remain either slow, destructive, or indirect. The current tools (e.g., Scholander pressure chamber, psychrometer, and pressure probe) involve disruption of the tissue, the microenvironment, or both (9–11). For example, the widely used pressure chamber requires excision of leaves or stems for the measurement of . Other techniques, such as stem and leaf psychrometry, require intimate contact with the tissue, and accurate and repeatable measurements are difficult to obtain (9, 12). These limitations have hindered the study of spatiotemporal water-potential gradients along the SPAC and the development of high-throughput strategies to phenotype based on tissue water potential (13). Additionally, current methods for measuring provide averages over tissues in the leaf. This characteristic makes the dissection of water relations on subleaf scales challenging, such that important questions remain, for example, about the partitioning of hydraulic resistances within leaves between the xylem and mesophyll (14–16).These outstanding challenges in the measurement of water status in planta motivated us to develop the measurement strategy presented here, AquaDust, with the following characteristics: 1) Minimally disruptive: Compatible with simple, rapid measurements on intact leaves. Fig. 1A presents our approach, in which AquaDust reporters infiltrated into the mesophyll of the leaf provide an externally accessible optical signal that correlates with the local water potential. 2) Localized: allowing for access to the values of water potential at a well-defined location along the path of transpiration in the leaf tissue. Fig. 1B shows a schematic representation of AquaDust particles localized in the apoplastic volume within the mesophyll, at the end of the hydraulic path for liquid water within the plant. 3) Sensitive and specific: capable of resolving water potentials across the physiologically relevant range ( MPa) and with minimal sensitivity to other physical (e.g., temperature) and chemical (e.g., pH) variables. Fig. 1C presents a schematic representation of an AquaDust particle formed of hydrogel, a highly tunable material that undergoes a structural response to changes in local water potential (swollen when wet; collapsed when dry). We couple the swelling behavior of the particle to an optical signal via the incorporation of fluorescence dyes (green and yellow circles in Fig. 1C) that undergo variable Förster Resonance Energy Transfer (FRET) as a function of spatial separation. Fig. 1D presents typical AquaDust spectra at high (wet; green curve) and low (dry; yellow curve) water potentials. A change in water potential leads to a change in the relative intensity of the two peaks in the AquaDust spectrum, such that the relative FRET efficiency, , can serve as a measure of water potential. 4) Inert: nondisruptive of the physiological properties of the leaf (e.g., photosynthetic capacity, transpiration rate, etc.).In this paper, we present the development, characterization, and application of AquaDust. We show that AquaDust provides a robust, reproducible response of its fluorescence spectra to changes in leaf water potential in situ and across the usual physiological range. We apply our approach to quantify the spatial gradients of water potential along individual leaves undergoing active transpiration and across a range of soil water potentials. With these measurements, we show that the localization of AquaDust in the mesophyll allows us to quantify the importance of hydraulic resistances outside the xylem. We further use AquaDust to measure the diurnal dynamics of under field conditions, with repeated measurements on individual, intact leaves. These measurements demonstrate the field-readiness of our techniques and validate the leaf hydraulic model we have developed. We conclude that AquaDust offers a powerful basis for tracking, spatially and temporally, water potential in planta to study the mechanisms by which it couples to both biological and physical processes to define plant function. 相似文献
72.
Jeff SW Wong Calvin SH Ng Tak Wai Lee Anthony PC Yim 《Canadian respiratory journal》2006,13(4):219-221
The present report describes a case of severe airway obstruction caused by endobronchial tuberculosis in an 11-year-old girl who was successfully treated by bronchoscopic balloon dilation. This case illustrates the insidious presentation and the increasingly important role of bronchoscopic intervention in the management of endobronchial tuberculosis. In addition, a brief literature review of the condition in the pediatric age group is included. 相似文献
73.
Woodberry T Suscovich TJ Henry LM Martin JN Dollard S O'Connor PG Davis JK Osmond D Lee TH Kedes DH Khatri A Lee J Walker BD Scadden DT Brander C 《The Journal of infectious diseases》2005,192(4):622-629
Cellular immune responses to Kaposi sarcoma-associated herpesvirus (KSHV), the etiological agent of KS and several other malignancies, are incompletely characterized. We assessed KSHV-specific interferon- gamma enzyme-linked immunospot responses in a cohort of 154 individuals, using overlapping peptide sets spanning the KSHV-encoded latency-associated nuclear antigen (ORF73) and the minor capsid glycoprotein (ORF65). Among KSHV-seropositive subjects, ORF73-specific responses dominated over responses to ORF65 and were preferentially detected in human immunodeficiency virus-coinfected individuals who had elevated levels of cell-associated KSHV DNA, indicating that the viral antigen burden may have been driving these responses. Responses to both ORF73 and ORF65 were also detected in several KSHV-seronegative subjects who were at increased risk for KSHV infection, which demonstrates that cellular immunity can be found in the absence of detectable humoral responses. These data have implications for the reliable identification of KSHV infection and may help guide the design of immune-based therapeutic and prophylactic interventions. 相似文献
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Estimating genome conservation between crop and model legume species 总被引:31,自引:0,他引:31
Choi HK Mun JH Kim DJ Zhu H Baek JM Mudge J Roe B Ellis N Doyle J Kiss GB Young ND Cook DR 《Proceedings of the National Academy of Sciences of the United States of America》2004,101(43):15289-15294
Legumes are simultaneously one of the largest families of crop plants and a cornerstone in the biological nitrogen cycle. We combined molecular and phylogenetic analyses to evaluate genome conservation both within and between the two major clades of crop legumes. Genetic mapping of orthologous genes identifies broad conservation of genome macrostructure, especially within the galegoid legumes, while also highlighting inferred chromosomal rearrangements that may underlie the variation in chromosome number between these species. As a complement to comparative genetic mapping, we compared sequenced regions of the model legume Medicago truncatula with those of the diploid Lotus japonicus and the polyploid Glycine max. High conservation was observed between the genomes of M. truncatula and L. japonicus, whereas lower levels of conservation were evident between M. truncatula and G. max. In all cases, conserved genome microstructure was punctuated by significant structural divergence, including frequent insertion/deletion of individual genes or groups of genes and lineage-specific expansion/contraction of gene families. These results suggest that comparative mapping may have considerable utility for basic and applied research in the legumes, although its predictive value is likely to be tempered by phylogenetic distance and genome duplication. 相似文献
77.
Lingfan Xu Yu Yin Yanjing Li Xufeng Chen Yan Chang Hong Zhang Juan Liu James Beasley Patricia McCaw Haoyue Zhang Sarah Young Jeff Groth Qianben Wang Jason W. Locasale Xia Gao Dean G. Tang Xuesen Dong Yiping He Daniel George Hailiang Hu Jiaoti Huang 《Proceedings of the National Academy of Sciences of the United States of America》2021,118(13)
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