全文获取类型
收费全文 | 404篇 |
免费 | 12篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 8篇 |
妇产科学 | 1篇 |
基础医学 | 92篇 |
口腔科学 | 4篇 |
临床医学 | 27篇 |
内科学 | 42篇 |
皮肤病学 | 3篇 |
神经病学 | 57篇 |
特种医学 | 2篇 |
外科学 | 39篇 |
预防医学 | 99篇 |
眼科学 | 3篇 |
药学 | 15篇 |
中国医学 | 1篇 |
肿瘤学 | 25篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 28篇 |
2020年 | 8篇 |
2019年 | 21篇 |
2018年 | 10篇 |
2017年 | 7篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 9篇 |
2013年 | 18篇 |
2012年 | 20篇 |
2011年 | 41篇 |
2010年 | 21篇 |
2009年 | 16篇 |
2008年 | 24篇 |
2007年 | 33篇 |
2006年 | 33篇 |
2005年 | 19篇 |
2004年 | 26篇 |
2003年 | 26篇 |
2002年 | 17篇 |
2001年 | 3篇 |
2000年 | 6篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1986年 | 1篇 |
排序方式: 共有419条查询结果,搜索用时 15 毫秒
61.
62.
63.
64.
65.
66.
67.
68.
69.
Kerjean A Couvert P Heams T Chalas C Poirier K Chelly J Jouannet P Paldi A Poirot C 《European journal of human genetics : EJHG》2003,11(7):493-496
In vitro folliculogenesis of cryopreserved ovarian tissue could be an effective method for insuring fertility for patients who receive gonadotoxic treatment. Although several culture systems have been described for growing female gametes in vitro, the production of competent oocytes for further development remains a considerable challenge. The purpose of our study was to determine whether maternal primary imprinting progresses normally during mouse oocyte growth in vitro. We analysed the DNA methylation status of differentially methylated regions of the imprinted genes H19, Mest/Peg1 and Igf2R using fully grown germinal vesicle-stage oocytes (fg oocytes) produced by in vitro folliculogenesis from early preantral follicles. When compared to fg oocytes removal from control females, we observed after in vitro development, a loss of methylation at the Igf2R locus in six out of seven independent experiments and Mest/Peg1 locus (one out of seven), and a gain of methylation at the H19 locus (one out of seven). These results provide insight into the dysregulation of the process of primary imprinting during oocyte growth in vitro and highlight the need for effective new biomarkers to identify complete nuclear reprogramming competence after in vitro folliculogenesis. 相似文献
70.
Bahi N Friocourt G Carrié A Graham ME Weiss JL Chafey P Fauchereau F Burgoyne RD Chelly J 《Human molecular genetics》2003,12(12):1415-1425
Previously, human genetics-based approaches allowed us to show that mutations in the IL-1 receptor accessory protein-like gene (IL1RAPL) are responsible for a non-specific form of X-linked mental retardation. This gene encodes a predicted protein of 696 amino acids that belongs to a novel class of the IL-1/Toll receptor family. In addition to the extracellular portion consisting of three Ig-like domains and the intracellular TIR domain characteristic of the IL-1/Toll receptor family, IL1RAPL contains a specific 150 amino acid carboxy terminus that has no significant homology with any protein of known function. In order to begin to elucidate the function of this IL-1/Toll receptor-like protein, we have assessed the effect of recombinant IL1RAPL on the binding affinity of type I IL-1R for its ligands IL-1alpha and beta and searched for proteins interacting with the specific carboxy terminus domain of IL1RAPL. Our results show that IL1RAPL is not a protein receptor for IL-1. In addition we present here the identification of Neuronal Calcium Sensor-1 (NCS-1) as an IL1RAPL interactor. Remarkably, although NCS-1 and its non-mammalian homologue, frequenin, are members of a highly conserved EF-hand Ca(2+) binding protein family, our data show that IL1RAPL interacts only with NCS-1 through its specific C-terminal domain. The functional relevance of IL1RAPL activity was further supported by the inhibitory effect on exocytosis in PC12 cells overexpressing IL1RAPL. Taken together, our data suggest that IL1RAPL may regulate calcium-dependent exocytosis and provide insight into the understanding of physiopathological mechanisms underlying cognitive impairment resulting from IL1RAPL dysfunction. 相似文献