全文获取类型
收费全文 | 805篇 |
免费 | 55篇 |
国内免费 | 16篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 43篇 |
妇产科学 | 3篇 |
基础医学 | 54篇 |
口腔科学 | 8篇 |
临床医学 | 86篇 |
内科学 | 183篇 |
皮肤病学 | 7篇 |
神经病学 | 15篇 |
特种医学 | 231篇 |
外科学 | 52篇 |
综合类 | 32篇 |
预防医学 | 40篇 |
眼科学 | 6篇 |
药学 | 88篇 |
中国医学 | 3篇 |
肿瘤学 | 23篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 12篇 |
2015年 | 11篇 |
2014年 | 7篇 |
2013年 | 15篇 |
2012年 | 13篇 |
2011年 | 15篇 |
2010年 | 23篇 |
2009年 | 31篇 |
2008年 | 20篇 |
2007年 | 24篇 |
2006年 | 23篇 |
2005年 | 23篇 |
2004年 | 12篇 |
2003年 | 25篇 |
2002年 | 27篇 |
2001年 | 7篇 |
2000年 | 16篇 |
1999年 | 21篇 |
1998年 | 46篇 |
1997年 | 50篇 |
1996年 | 38篇 |
1995年 | 23篇 |
1994年 | 41篇 |
1993年 | 27篇 |
1992年 | 14篇 |
1991年 | 16篇 |
1990年 | 14篇 |
1989年 | 31篇 |
1988年 | 23篇 |
1987年 | 19篇 |
1986年 | 25篇 |
1985年 | 15篇 |
1984年 | 20篇 |
1983年 | 13篇 |
1982年 | 14篇 |
1981年 | 25篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1975年 | 10篇 |
1970年 | 2篇 |
排序方式: 共有876条查询结果,搜索用时 15 毫秒
21.
Kholoud K. Khoja Melanie-Jayne R. Howes Robert Hider Paul A. Sharp Iain W. Farrell Gladys O. Latunde-Dada 《Nutrients》2022,14(4)
Trigonella foenum-graecum L. (fenugreek), a member of the legume family (Fabaceae), is a promising source of bioactive phytochemicals, which explains its traditional use for a variety of metabolic disorders including cancer. The current study aimed to evaluate extracts of fenugreek seeds and sprouts, and some of their constituents, to compare their cytotoxic and antiproliferative activities in MCF-7 breast cancer cells. The extracts were chemically characterised using high-resolution accurate mass liquid chromatography-mass spectrometry to reveal the detection of compounds assigned as flavone C-glycosides including those derived from apigenin and luteolin, in addition to isoflavones. Five different flavones or their glycosides (apigenin, vicenin-2, vitexin, luteolin and orientin) and two isoflavones (daidzein and formononetin) were quantified in the fenugreek extracts. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using MCF-7 cells treated with fenugreek methanolic extracts showed dose- and time-dependent effects on cell viability. The MCF-7 cancer cells treated with the fenugreek methanolic extracts also displayed increased relative mitochondrial DNA damage as well as suppressed metastasis and proliferation. This study demonstrates the potential anti-cancer effects of fenugreek seeds and sprouts and reveals fenugreek sprouts as an untapped resource for bioactive compounds. 相似文献
22.
The mean maximum nuclear diameter (Dmax) in 21 cases of non-Hodgkin's lymphoma (NHL) has been determined, using the Reichert-Jung (Kontron) MOP-AMO3 user-controlled image analyser. Nuclear diameters of high-grade malignancy NHL were found to be considerably greater than those of low-grade malignancy lymphomas, although there was some overlap of their ranges. These findings confirm objectively subjective estimates of nuclear size in NHL. The relative usefulness of the user-controlled (interactive) image analyser for the measurement of nuclei in tissue sections is compared with that of a fully automatic machine. 相似文献
23.
Continuous Cardiac Monitoring around Atrial Fibrillation Ablation: Insights on Clinical Classifications and End Points 下载免费PDF全文
24.
First rotavirus vaccine licensed: Is there really a need? 总被引:6,自引:0,他引:6
RI Glass JS Bresee UD Parashar RC Holman JR Gentsch 《Acta paediatrica (Oslo, Norway : 1992)》1999,88(S426):2-8
The first rotavirus vaccine was licensed in the United States on 31 August 1998 for the prevention of severe rotavius diarrhea in children. Despite this landmark in new vaccines, many pediatricians and public health professionals in Europe are uncertain of the need for this vaccine for the routine immunization of infants. In Europe, ample evidence suggests that rotavirus is the most common cause of hospitalizations for severe diarrhea among children, but proper studies documenting the disease burden of rotavirus or th cost-effectiveness of a rotavirus immunization program have only been conducted in the United Kingdom following epidemiologic models used in the United States. All children are infected with rotavirus during their first few years of life, 30-50% of diarrheal hospitalizations among children <5 years are due to this agent, and, by the age of 5 years, between 1 in 40 and 1 in 77 children in Europe and the United States may be hospitalized for rotavirus. The first vaccine is a live, oral preparation combining four different serotypes of rotavirus and administered in three doses with other childhood immunizations. The good efficacy against severe rotavirus diarrhea, the low risk of adverse side effects and the positive costeffectiveness equation have led the two major immunization advisory groups in the U.S. to recommend this vaccine for routine use in American infants. European physicians and policymakers should re-examine the epidemiology and disease burden of rotavirus diarrhea now that an effective method of prevention is at hand. □ Childhood immunization, diseases, rotavirus, vaccination . 相似文献
25.
PTEN is inversely correlated with the cell survival factor Akt/PKB and is inactivated via multiple mechanismsin haematological malignancies 总被引:7,自引:1,他引:7
26.
P Rump RC Niessen KT Verbruggen OF Brouwer M de Raad R Hordijk 《Clinical genetics》2011,79(2):183-188
Rump P, Niessen RC, Verbruggen KT, Brouwer OF, de Raad M, Hordijk R. A novel mutation in MED12 causes FG syndrome (Opitz–Kaveggia syndrome). Opitz–Kaveggia syndrome is a rare X‐linked multiple congenital anomalies and intellectual disability disorder caused by the recurrent p.R961W mutation in the MED12 gene. Twenty‐three affected males from 10 families with this mutation in the MED12 gene have been described so far. Here we report on a new family with three affected cousins, in which we identified a novel MED12 mutation (p.G958E). This is the first demonstration that other mutations in this gene can also lead to Opitz–Kaveggia syndrome. The clinical phenotype of these three new cases is reviewed in detail and compared with the previous reported cases. 相似文献
27.
KJ Champion C Bunag AL Estep JR Jones CH Bolt RC Rogers KA Rauen DB Everman 《Clinical genetics》2011,79(5):468-474
Champion KJ, Bunag C, Estep AL, Jones JR, Bolt CH, Rogers RC, Rauen KA, Everman DB. Germline mutation in BRAF codon 600 is compatible with human development: de novo p.V600G mutation identified in a patient with CFC syndrome. BRAF, the protein product of BRAF, is a serine/threonine protein kinase and one of the direct downstream effectors of Ras. Somatic mutations in BRAF occur in numerous human cancers, whereas germline BRAF mutations cause cardio‐facio‐cutaneous (CFC) syndrome. One recurrent somatic mutation, p.V600E, is frequently found in several tumor types, such as melanoma, papillary thyroid carcinoma, colon cancer, and ovarian cancer. However, a germline mutation affecting codon 600 has never been described. Here, we present a patient with CFC syndrome and a de novo germline mutation involving codon 600 of BRAF, thus providing the first evidence that a pathogenic germline mutation involving this critical codon is not only compatible with development but can also cause the CFC phenotype. In vitro functional analysis shows that this mutation, which replaces a valine with a glycine at codon 600 (p.V600G), leads to increased ERK and ELK phosphorylation compared to wild‐type BRAF but is less strongly activating than the cancer‐associated p.V600E mutation. 相似文献
28.
Laura A Hawryluck William RC Harvey Louise Lemieux-Charles Peter A Singer 《BMC medical ethics》2002,3(1):3-9
Background
Intensivists must provide enough analgesia and sedation to ensure dying patients receive good palliative care. However, if it is perceived that too much is given, they risk prosecution for committing euthanasia. The goal of this study is to develop consensus guidelines on analgesia and sedation in dying intensive care unit patients that help distinguish palliative care from euthanasia.Methods
Using the Delphi technique, panelists rated levels of agreement with statements describing how analgesics and sedatives should be given to dying ICU patients and how palliative care should be distinguished from euthanasia. Participants were drawn from 3 panels: 1) Canadian Academic Adult Intensive Care Fellowship program directors and Intensive Care division chiefs (N = 9); 2) Deputy chief provincial coroners (N = 5); 3) Validation panel of Intensivists attending the Canadian Critical Care Trials Group meeting (N = 12).Results
After three Delphi rounds, consensus was achieved on 16 statements encompassing the role of palliative care in the intensive care unit, the management of pain and suffering, current areas of controversy, and ways of improving palliative care in the ICU.Conclusion
Consensus guidelines were developed to guide the administration of analgesics and sedatives to dying ICU patients and to help distinguish palliative care from euthanasia. 相似文献29.
30.
Joyce J.C. Kroot Erwin H.J.M. Kemna Sukhvinder S. Bansal Mark Busbridge Natascia Campostrini Domenico Girelli Robert C. Hider Vasiliki Koliaraki Avgi Mamalaki Gordana Olbina Naohisa Tomosugi Chris Tselepis Douglas G. Ward Tomas Ganz Jan C.M. Hendriks Dorine W. Swinkels 《Haematologica》2009,94(12):1748-1752
The recently discovered iron regulatory peptide hormone hepcidin holds promise as a novel biomarker in iron metabolism disorders. To date, various mass spectrometry and immunochemical methods have been developed for its quantification in plasma and urine. Differences in methodology and analytical performance hinder the comparability of data. As a first step towards method harmonization, several hepcidin assays were compared. Worldwide eight laboratories participated in a urinary and plasma round robin in which hepcidin was analyzed. For both urine and plasma: (i) the absolute hepcidin concentrations differed widely between methods, (ii) the between-sample variation and the analytical variation of the methods are similar. Importantly, the analytical variation as percentage of the total variance is low for all methods, indicating their suitability to distinguish hepcidin levels of different samples. Spearman correlations between methods were generally high. The round robin results inform the scientific and medical community on the status and agreement of the current hepcidin methods. Ongoing initiatives should facilitate standardization by exchanging calibrators and representative samples. 相似文献