A virologic pilot study of valacyclovir in infectious mononucleosis.

BACKGROUND: Infectious mononucleosis decreases the productivity of many college students and Epstein-Barr virus (EBV) infection may result in long-term immune damage. OBJECTIVES: Evaluate the antiviral effect of valacyclovir during EBV-related acute infectious mononucleosis and explore potential clinical benefits. STUDY DESIGN: University students who presented during the first 7 days of illness were randomized to receive valacyclovir 3g/day for 14 days or not. The quantity of Epstein-Barr virus (EBV) DNA in oral and whole blood samples was determined by real-time (TaqMan) PCR. The primary outcome was the proportion of subjects with laboratory-confirmed primary EBV infection who had >or=2 log10 decrease in EBV copies/mL in oral washes during the treatment period. Secondary outcomes included clinical effects. RESULTS: Twenty subjects were studied. The proportion of valacyclovir recipients versus control subjects who had >or=2 log10 decrease in EBV copies was significantly greater for both oral wash fluid-derived cell pellet (P=0.03) and supernatant (P=0.001) samples. At the end of the treatment period, the number of reported symptoms (P=0.03) and the severity of illness (P=0.049) were reduced among valacyclovir recipients as compared with controls. CONCLUSIONS: Valacyclovir therapy caused a reduction of EBV excretion and possibly produced a clinical benefit in infectious mononucleosis. Because our study was small and not placebo-controlled, these results must be confirmed by a larger, placebo-controlled trial.     

Forensic age assessment by 3.0T MRI of the knee: proposal of a new MRI classification of ossification stages

Purpose

To explore the possibility of determining majority via a morphology-based examination of the epiphyseal-diaphyseal fusion by 3.0 T magnetic resonance imaging (MRI), a prospective cross-sectional study developing and applying a new stage classification was conducted.

Materials and methods

344 male and 350 female volunteers of German nationality between the ages of 12-24 years were scanned between May 2013 and June 2015. A 3.0 T MRI scanner was used, acquiring a T1-weighted (T1-w) turbo spin-echo sequence (TSE) and a T2-weighted (T2-w) TSE sequence with fat suppression by spectral pre-saturation with inversion recovery (SPIR). The gathered information was sifted and a five-stage classification was formulated as a hypothesis. The images were then assessed using this classification. The relevant statistics were defined, the intra- and interobserver agreements were determined, and the differences between the sexes were analysed.

Results

The application of the new classification made it possible to correctly assess majority in both sexes by the examination of the epiphyses of the knee joint. The intra- and interobserver agreement levels were very good (κ > 0.80). The Mann-Whitney-U Test implied significant sex-related differences for most stages.

Conclusion

Applying the presented MRI classification, it is possible to determine the completion of the 18th year of life in either sex by 3.0 T MRI of the knee joint.

Key points

? Based on prospective referential data a new MRI classification was formulated. ? The setting allows assessment of the age of an individual’s skeletal development. ? The classification scheme allows the reliable determination of majority in both sexes. ? The staging shows a high reproducibility for instructed and trained professional personnel. ? The proposed classification is likely to be adaptable to other long bone epiphyses.

     

Opioid sedation does not alter intracranial pressure in head injured patients

Purpose

This study aimed to examine the effects of sedative doses of morphine, fentanyl and sufentanil on intracranial pressure (ICP) in head-injured patients in whom changes in mean artenal pressure (MAP) were minimized.

Methods

Fifteen severely head-injured patients (G5C of ≤8) were randomly assigned to receive either fentanyl. sufentanil or morphine, titrating the drug to a maximal 10% decrease in MAP. The patients were subsequently given an infusion of the same opioid. For four hours, ICP MAP and heart rate were recorded. Results: In all groups, there were no increases in ICP. There was a decrease in MAP in the sufentanil group at 10 min (P < 0.05) and 45 min after the initial opioid bolus. These decreases in MAP were not associated with increases in ICP.

Conclusion

The study suggests that when opioids are titrated in head-injured patients, worsening intracranial pressure can be avoided.     

Anatomic shape variants of extremitas sternalis claviculae as collected from sternoclavicular thin-slice CT-studies of 2820 male borderline-adults

International Journal of Legal Medicine - Within medical age assessment practice, the indicator “medial clavicular ossification” constitutes crucial evidence capable of excluding age...     

Magnetic resonance imaging of the iliac crest: age estimation in under-20 soccer players

Age assessment of living individuals represents a valuable tool in both forensic medicine and sports medicine. In soccer, age-related tournaments play an important role in guaranteeing equal chances to the competitors. However, age estimations in this field should not rely on imaging methods that include exposure to radiation. Therefore, the present study investigates the possibilities of magnetic resonance imaging (MRI) of the iliac crest apophysis for the purpose of evaluating skeletal maturation in under-20 (U-20) soccer players. To this end, gradient echo 3D sequences of the whole pelvis of 152 male tournament soccer players between 18 and 22 years of age were prospectively evaluated. A four stage classification system was applied for the assessment of the apophyseal ossification. Reliable stage determination was possible in all cases. Further sub-classification did not appear feasible due to the limitations of MRI. Analysis of the statistical parameters showed that age medians increased steadily from stage to stage. However, they did not allow for further differentiation of skeletal maturity in the cohort studied. Thus, MRI of the iliac crest appears to be generally suitable in age diagnostics of living individuals, but further investigations, especially in a cohort of individuals aged between 10 and 20 years, are needed in order to establish this method as novel criterion in sports or forensic medicine.     

Dental age diagnostics by means of radiographical evaluation of the growth stages of lower wisdom teeth

The main criterion for dental age estimation in living adolescents and young adults is mineralization of third molars. A total of 1,260 orthopantomograms of 669 female and 591 male subjects aged between 15 and 22 years was examined. Of the orthopantomograms, 1,137 were from patients with Swiss citizenship and 123 were from other European countries. The mineralization status of lower third molars was evaluated based on Demirjian's classification of stages. For the statistical assessment of data, logistic regression and mean value testing were used. The findings show that completed growth in wisdom teeth is observable at ages less than 18. Male individuals were approximately 1 year ahead of females at stage H, and South East Europeans were approximately 6 months ahead of Swiss (Central Europe) at stage H. Mean values led to false conclusions regarding the question of the attained age of 18. Therefore, regression analysis should be used for the statistical assessment of data in dental age diagnostics.     

Beurteilung der Weisheitszahneruption

A criterion in dental age diagnostics is assessment of the eruption stage of the third molars. As the reliability of an age diagnosis is crucially dependent on the stage classification used, the most suitable system should be implemented for age estimation purposes. The objective of the present study was to compare the reliability of two classifications for radiological assessment of third molar eruption described by Olze et al. (Int J Legal Med 2007, 121:445?C448; Arch Kriminol 2012, 229:145?C153). To that end 216 conventional orthopantomograms of German males and females in the age group between 15 and 25 years were evaluated by two observers (one dentist with 20 years professional experience and one dentist with 6 years professional experience). The weighted kappa coefficient was used to determine intraobserver and interobserver agreement, whilst the eta coefficient was determined as a measure of the correlation between age and eruption stage. Based on the synopsis of the calculated kappa and eta coefficients it was concluded that both stage classifications for determining the developmental status of third molar eruption under study can, in principle, be recommended for age estimation purposes as they both command similar high levels of precision and accuracy. Stage classification II appears particularly suitable for inexperienced examiners and for assessing X-ray images on which the gingiva is not clearly definable.     

Beurteilung des Ossifikationsstadiums der medialen Klavikulaepiphysenfuge

In forensic age estimation of the living in criminal proceedings the radiological examination of the ossification status of the medial clavicular epiphysis permits an assessment with regard to the completion of 21 years of age. In the present study the authors prospectively comparatively analyzed 8 prepared samples of the sternoclavicular region of individuals aged 15–17 years obtained during autopsy by means of projectional radiography, thin-slice computed tomography and magnetic resonance imaging. In 6 out of 15 examined sternoclavicular joints the ossification stage of the medial clavicular epiphysis was in agreement in each of the three imaging methods used. In the remaining cases the ossification stage was assessed as either one stage higher or lower in one of the imaging methods than in the other two techniques. In five cases compared to CT, and seven cases compared to MR, projectional radiography showed a higher ossification stage than CT and MR imaging. In two cases projectional radiography resulted in the determination of a lower stage than in CT and MR imaging. MR scans showed a less advanced ossification stage than CT-based images in two cases. In the practice of age estimation modality-specific reference studies based on projectional radiography and computed tomography are to be applied in order to guarantee an adequate assessment of the ossification stage of the medial clavicular epiphysis.