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51.
Claudio Gustavo Stefanoff Rocío Hassan Ana Carolina Gonzalez Luiz Ant?nio B Andrade Daniel G Tabak Sérgio Romano Ilana R Zalcberg 《Diagnostic molecular pathology》2003,12(2):79-87
We herein present a technical strategy to optimize DNA isolation from paraffin-embedded tissue (PET). This includes the choice of adequate buffers for proteinase K digestion and multiplex PCR amplifications for assessing the appropriateness of DNA extracts for subsequent PCR assays for detecting clonality. We found that the association of proteinase K digestion in nonionic buffer and subsequent extract dilutions accounted for 79% of successful amplifications. A final efficiency of 88% was achieved by additional organic extractions and/or re-extractions. Comparisons were carried out with control DNA extracts from fresh samples to assess the efficiency of each clonality assay. Immunoglobulin CDRIII rearranged region amplification was more efficient for pregerminal center B-cell lymphomas in contrast to CDRII rearrangement detection, which was more effective for germinal and postgerminal lymphomas. T-cell clonality detection by TCRgamma PCR was less efficient in PET samples than in fresh tissues showing that DNA integrity is more critical for TCR than for IGH amplification. Two inconclusive cases without phenotypic markers and two other atypical lymphoproliferations masked by reactive T cells were diagnosed as plasmablastic lymphomas and as monoclonal B-proliferations, respectively, due to IGH rearrangements. 相似文献
52.
Models of hypersensitivity pneumonitis (HP) should exhibit progression of pulmonary histological abnormalities during continuing challenges. Strain II guinea-pigs were sensitized with Micropolyspora faeni and received 2, 4, or 8 weekly intratracheal (i.t.) particulate M. faeni challenges. Control animals received normal saline (NS). Four days after the last exposure, randomly selected microscopic fields of lung (200/animal) were judged to be normal or abnormal. If abnormal, the location and nature of the abnormalities were determined. Compared with NS treated guinea-pigs, those exposed to 2, 4 and 8 weekly M. faeni challenges exhibited more extensive (P less than 0.001) pulmonary histological abnormalities which involved both the intraalveolar and interstitial compartments. More extensive abnormalities in the 8 week group compared with the 4 week group were caused by increased extent of interstitial mononuclear cell infiltration. The extent of pulmonary interstitial histological abnormalities transiently (four challenges) decreases, but then increases, so that progressive pulmonary inflammation occurs during continuing challenges. 相似文献
53.
An immunoblotting technique was modified to detect and biochemically characterize HLA-DR antigens expressed on circulating human monocytes. Membrane proteins of peripheral blood monocytes were solubilised using the mildly anionic detergent, sodium deoxycholate. These solubilised proteins were resolved by SDS-PAGE and transferred electrophoretically to nitrocellulose. The HLA-DR antigen was detected using a polyclonal antiserum and two monoclonal anti-HLA-DR antibodies. Both immunoperoxidase and 125 I autoradiography techniques were used for visualisation of the antigen. The resolution of HLA-DR reactive material was increased when proteins were renatured with 4M urea after blotting. Immunoprobing of a sample of solubilized membrane proteins showed three bands of HLA-DR antigenic reactivity at molecular weights 65kDa, 55kDa and 46kDa. After storage at –70°C for 2 months, only the 46kDa HLA-DR antigen band was detectable. Nonetheless, the 2-chain HLA-DR molecule was found to be an extremely stable complex which could not be dissociated by boiling in sample buffer containing 5% 2-mercaptoethanol and 2% SDS. A stronger reducing agent, 25 mM dithiotreitol, was required to split the HLA-DR molecule into its alpha and beta subunit chains. Finally, in a study of circulating monocytes from normal subjects, the immunoblotting technique was shown to quantitate solubilised HLA-DR antigen in a reproducible manner. 相似文献
54.
Immunomodulation of autoimmune and inflammatory diseases with intravenous immunoglobulin 总被引:2,自引:0,他引:2
Ephrem A Misra N Hassan G Dasgupta S Delignat S Duong Van Huyen JP Chamat S Prost F Lacroix-Desmazes S Kavery SV Kazatchkine MD 《Clinical and experimental medicine》2005,5(4):135-140
Abstract Intravenous immunoglobulin (IVIg) has been used in the treatment of primary and secondary antibody deficiencies for over two
decades. Since the early 1980s, the therapeutic efficacy of IVIg has been established in idiopathic thrombocytopenic purpura,
Guillain-Barré syndrome, chronic inflammatory demyelinating polyneuropathy, myasthenia gravis, dermatomyositis and Kawasaki
syndrome, and the prevention of graft versus host disease in recipients of allogeneic bone marrow transplants. Its use has
also been reported in a large number of other autoimmune and systemic inflammatory conditions. In this review, we discuss
the mechanisms by which IVIg exerts immunomodulatory effects in immune pathologies. 相似文献
55.
Defining epithelial cell progenitors in the human oxyntic mucosa 总被引:7,自引:0,他引:7
In the human stomach, the oxyntic epithelium includes numerous tubular invaginations consisting of short pits opening into long glands. The pit is lined by pit cells, whereas the gland is composed of three regions: the base, containing zymogenic cells; the neck, containing neck cells; and the isthmus, composed of little known immature cells and of parietal cells, which are also scattered through the neck and base. The aim of this study was to examine the ultrastructure of the immature cells and to determine their relation to mature cells. To do so, normal oxyntic mucosal biopsies from subjects ranging from 20-43 years old were fixed in aldehydes and postfixed in reduced osmium for electron microscopy and morphometric analysis. The immature cells were sorted out into four classes, whose roles were clarified by comparison with the thoroughly investigated mouse oxyntic epithelium. The first class was composed of the least differentiated immature cells, which were rare and characterized by minute, dense, or cored secretory granules and were accordingly named mini-granule cells. Their function was not clarified. The second class consisted of pre-pit cells, which were characterized by few dense mucous granules and give rise to pit cells that ascend the pit wall and, after reaching the luminal surface, die or are extruded. Both pre-pit and pit cells underwent continuous renewal and, therefore, together constituted a renewal system referred to as pit cell lineage. The third class, or pre-neck cells, characterized by cored secretory granules, give rise to neck cells that descend toward the base region and differentiate further into pre-zymogenic cells, which finally become zymogenic cells. The latter eventually degenerate and die. Thus pre-neck cells and their progeny constitute a renewing system, designated zymogenic cell lineage. The fourth class, or pre-parietal cells, characterized by long microvilli and few tubulovesicles, differentiate into parietal cells that descend along the neck and base regions and eventually degenerate and die. Pre-parietal and parietal cells represent a renewing system referred to as parietal cell lineage. While the origin of the last three classes of progenitor cells has not been elucidated, it is likely that they arise either from an unidentified multipotential stem cell, possibly the mini-granule cell itself, or from the mitotic activity of pre-pit and pre-neck cells. In conclusion, the human oxyntic epithelium is composed of continually renewing cells organized in distinct cell lineages. 相似文献
56.
Stanton WR Saleheen HN O'Riordan D Roy CR 《International journal of behavioral medicine》2003,10(4):285-298
Sun exposure in childhood is 1 of the risk factors for developing skin cancer, yet little is known about levels of exposure
at this age. This is particularly important in countries with high levels of ultraviolet radiation (UVR) such as Australia.
Among 49 children 3 to 5 years of age attending child care centers, UVR exposure was studied under 4 conditions in a repeated
measures design; sunny days, cloudy days, teacher’s instruction to stay in the shade, and a health professionals instruction
to apply sunscreen. Three different data collection methods were employed: (a) completion of questionnaire or diary by parents
and researcher, (b) polysulphone dosimeter readings, and (c) observational audits (video recording).
Results of this study indicated that more than half the children had been sunburnt (pink or red) and more than a third had
experienced painful sunburn (sore or tender) in the last summer. Most wore short sleeve shirts, short skirts or shorts and
cap, that do not provide optimal levels of skin protection. However, sunscreen was applied to all exposed parts before the
children went out to the playground. Over the period of 1 hr (9–10 a.m.) the average amount of time children spent in full
sun was 22 min. On sunny days there was more variation across children in the amount of sun exposure received. While the potential
amount of UVR exposure for young children during the hour they were outside on a sunny day was 1.45 MED (Minimum Erythemal
Dose), they received on average 0.35 MED, which is an insufficient amount to result in an erythemal response on fair skin
even without the use of sunscreen. 相似文献
57.
Acute hypervolemia does not improve arterial oxygenation in maximally exercising thoroughbred horses
Recently, it was reported that acute hypervolemia improves arterial oxygen tension in human athletes known to experience exercise-induced arterial hypoxemia. Since exercise-induced arterial hypoxemia is routinely observed in racehorses and is known to limit performance, we examined whether pre-exercise induction of acute hypervolemia would similarly benefit arterial oxygenation in maximally exercising thoroughbred horses. Two sets of experiments, namely, placebo [intravenous (IV) physiological saline] and acute hypervolemia (IV 7.2% NaCl, causing an 18.2% expansion of plasma volume) studies were carried out in random order on 13 healthy, exercise-trained thoroughbred horses, 7 days apart. An incremental exercise protocol leading to 120 s of galloping at 14 m s–1 on a 3.5% uphill incline was used. Galloping at this workload elicited maximal heart rate and induced pulmonary hemorrhage in all horses in both treatments. In the placebo study, arterial oxygen tension decreased to 76.1 (2) mmHg (P<0.0001) at 30 s of maximal exertion, but further significant changes did not occur as exercise duration increased to 120 s [arterial oxygen tension 72.4 (2) mmHg]. A significant arterial hypoxemia also developed in galloping horses in the acute hypervolemia study [arterial oxygen tension at 30 and 120 s was 76.7 (1.7) and 71.9 (1.6) mmHg, respectively], but significant differences between treatments could not be demonstrated. In both treatments, a similar desaturation of arterial hemoglobin was also observed at 30 s of maximal exercise, which intensified with increasing exercise duration as hyperthermia, acidosis and hypercapnia intensified. Thus, acute expansion of plasma volume did not benefit arterial oxygenation in maximally exercising thoroughbred horses. 相似文献
58.
Toxicologic analysis cannot supplant physician skills in the diagnosis and management of poisoning; however, it is a useful adjunct when properly used. Laboratory use should reflect critical consideration of clinical contribution as well as insight into institutional capability. A detailed historic review and interview of multiple sources may provide more useful and expeditious information than the blind "drug screen." Test ordering should be limited to that which directly contributes to clinical patient management.16 Similarly, test results must be interpreted in the clinical context of patient presentation. The reported units of measurement must be carefully scrutinized; and the potential for laboratory error must be appreciated. Most importantly, communication and cooperation must be maintained between the physician and laboratory personnel if the resource is to be optimally used. 相似文献
59.
Ladislav Novotný Hassan Farghali Miloš Ryba Ivo Janků Jiří Beránek 《Cancer chemotherapy and pharmacology》1984,13(3):195-199
Summary Both transport and biotransformation processes for a series of pyrimidine nucleobases, ribonucleosides, 2-deoxyribonucleosides, and acetyl and 5-substituted derivatives of the cancerostatic agent araC were studied in the isolated everted rat jejunum with a continuous perfusion technique. Metabolic alterations during penetration were assessed by HPLC. 5-Halogeno and 5-deoxy derivatives of cytosine nucleosides exhibited higher transport rates and higher stability towards the deamination reaction than did unsubstituted derivatives. Octanol-buffer partition coefficients were estimated for the study compounds, and fragmental constants for the sugar moieties of nucleosides were assessed. With the present study compounds there was no correlation between lipophilicity and transport rate, as previously reported, but there was a correlation between lipophilicity and metabolic alteration of araC derivatives (r=0.99, n=5). 相似文献
60.
A rapid assay is described for the simultaneous determination of emetine and cephaeline in ipecacuanha and its preparations, based upon the different fluorescence intensities of the alkaloids at pH 1 and pH 12. The assay involves the measurement of fluorescence at 317 nm of dilutions of the sample in 0.1 M hydrochloric acid (pH 1) and 0.01 M sodium hydroxide (pH 12) with an excitation wavelength of 283 nm. Concentrations of the individual alkaloids are calculated using two simultaneous equations derived from the experimentally determined coefficients of fluorescence of solutions (1 μg/ml) of emetine and cephaeline at pH 1 and pH 12. The procedure, which has been shown to be accurate, precise and sensitive, requires only 1 ml of a liquid sample and less than 0.5 g of powdered root. There was reasonable agreement between the total concentrations of alkaloids in tinctures and liquid extracts of ipecacuanha determined by the spectrofluorimetric method and by the titrimetric procedure of the British Pharmacopoeia. The fluorimetric procedure gave higher levels of alkaloids in the powdered root than did the B.P. method; this difference is explained by incomplete extraction of the alkaloids in the B.P. procedure for powdered ipecacuanha root. 相似文献