高压氧预处理对宫内缺血缺氧大鼠脑组织及血浆神经肽Y含量的影响

目的 :探讨高压氧 (HBO)预处理对宫内缺血缺氧大鼠脑组织及血浆神经肽Y含量的影响。方法 :采用放射免疫法测定 2 5例正常胎鼠 (对照组 )、2 7例宫内缺血缺氧胎鼠 (缺血缺氧组 )及 1 9例经高压氧预处理宫内缺血缺氧胎鼠 (HBO组 )中枢及外周血神经肽Y的含量。结果 :HBO组胎鼠脑及外周血神经肽Y的含量分别为 52 .64±1 2 .1 6pg/mg及 1 99.51± 49.36ng/L ,缺血缺氧组胎鼠脑及外周血神经肽Y的含量分别为87.2 5± 2 3.63pg/mg及 2 51 .53±55 .2 8ng/L ,对照组胎鼠神经肽Y含量为 48.63± 1 0 .72pg/mg及 1 4 7.35± 31 .49ng/L ;与对照组相比 ,缺血缺氧组胎鼠中枢及外周神经肽Y含量均显著升高 (P <0 .0 1 ) ;HBO组神经肽Y含量显著低于缺血缺氧组 (P <0 .0 5)。结论 :神经肽Y与围生期缺血缺氧的发生密切相关 ,HBO预处理具有改善宫内缺血缺氧的作用 ,其机理可能与抑制神经肽Y的生成有关     

解密抑郁症

来自美国、瑞典及法国的科学家发现，有一种蛋白可能在抑郁症的发病过程中起着极为重要的作用。他们希望这一发现将有助于明确抑郁症的病因，找到新的治疗方法（Svenningsson P et al．Sc／ence．2006：311：77—80）。     

CCD二极管阵列检测-流动注射分析尿中的肌酐

目的：研究流动注射-CCD二极管阵列检测分光光度法测定尿中肌酐含量的方法。方法：基于经典的Jaffe反应，肌酐能与碱性苦味酸生成橙红色复合物，利用化学动力学反应，采用CCD二极管阵列检测装置快速测定尿中肌酐的含量。本实验探讨了最佳反应条件和流动注射分析参数。结果：本方法的线性范围为1μg／ml～33μg／ml，检出限为1μg／ml。样品的加标回收率在81．63％～119．7％之间，精密度为2．74％～3．96％。将本方法与临床自动生化仪的测定结果比较，经t检验（t=0．179，P〉0．05），两种方法测定结果无统计学差异。结论：本方法采样频率为20次／11，简便快速、分析成本低，实现了尿中肌酐含量的批样分析，获得了满意的结果。     

实验动物和脏器标本的摄影方法

文章结合实践介绍了实验动物和脏器标本的摄影方法及注意事项,为从事这方面工作的科技人员提供了技术支持。     

Expression of cytochrome p450 and other biotransformation genes in fetal and adult human nasal mucosa.

Despite recent progress in the identification and characterization of numerous nasal biotransformation enzymes in laboratory animals, the expression of biotransformation genes in human nasal mucosa remains difficult to study. Given the potential role of nasal biotransformation enzymes in the metabolism of airborne chemicals, including fragrance compounds and therapeutic agents, as well as the potential interspecies differences between laboratory animals and humans, it would be highly desirable to identify those biotransformation genes that are expressed in human nasal mucosa. In this study, a global gene expression analysis was performed to compare biotransformation enzymes expressed in human fetal and adult nasal mucosa to those expressed in liver. The identities of a list of biotransformation genes with apparently nasal mucosa-selective expression were subsequently confirmed by RNA-polymerase chain reaction (PCR) and DNA sequencing of the PCR products. Further quantitative RNA-PCR experiments indicated that, in the fetus, aldehyde dehydrogenase 6 (ALDH6), CYP1B1, CYP2F1, CYP4B1, and UDP glucuronosyltransferase 2A1 are expressed preferentially in the nasal mucosa and that ALDH7, flavin-containing monooxygenase 1, and glutathione S-transferase P1 are at least as abundant in the nasal mucosa as in the liver. The nasal mucosal expression of CYP2E1 was also detected. These findings provide a basis for further explorations of the metabolic capacity of the human nasal mucosa for xenobiotic compounds.     

穿心莲内酯微囊的制备及溶出度考察

目的:研究以丙烯酸树脂Ⅳ号为囊材的穿心莲内酯微囊的制备方法及其体外溶出特点.方法:采用液中干燥法制备穿心莲内酯微囊,并按<中国药典>方法测定体外溶出度.结果:该法制备的穿心莲内酯微囊,在水中药物几乎没有溶出,而在酸性溶出介质中则可快速释放.结论:该方法所制备微囊不仅可以有效掩盖穿心莲内酯的苦味,而且在胃液中能够快速释放.     

德国柏林Pflgewerk养老机构学习见闻分享

2019年9—12月,笔者参与了苏州大学与德国柏林Pflgewerk养老机构针对失能老年人居家照护课程研发与智慧化养老的交流项目,在学习与交流期间,德国完善的居家照护体系,先进适老辅具的研发与运用,系统规范的照护员培训课程,浓厚的人文关怀气氛与多学科团队合作的安宁疗护中心令笔者印象深刻。本文结合学习交流活动的亲身经历,...     

The altering in sensory sensitivity: a current issue of female breast surgery

Breast surgery is an important treatment for women with malignant breast diseases. In addition to breast appearance, the integrity of breast function is increasing in patients with breast diseases. As the basis of breast physiological function, breast skin sensitivity is important to the quality of life of patients after surgery. Breast skin sensitivity gives the patient a “real” breast feeling. The sensory recovery after breast surgery has also become one of the important goals of breast surgery. In this review, we aim to discuss the research progress on recovery of breast skin sensitivity after different treatment modalities for breast disease.     

Catechins as a Potential Dietary Supplementation in Prevention of Comorbidities Linked with Down Syndrome

Plant-derived polyphenols flavonoids are increasingly being recognized for their medicinal potential. These bioactive compounds derived from plants are gaining more interest in ameliorating adverse health risks because of their low toxicity and few side effects. Among them, therapeutic approaches demonstrated the efficacy of catechins, a major group of flavonoids, in reverting several aspects of Down syndrome, the most common genomic disorder that causes intellectual disability. Down syndrome is characterized by increased incidence of developing Alzheimer’s disease, obesity, and subsequent metabolic disorders. In this focused review, we examine the main effects of catechins on comorbidities linked with Down syndrome. We also provide evidence of catechin effects on DYRK1A, a dosage-sensitive gene encoding a protein kinase involved in brain defects and metabolic disease associated with Down syndrome.