Up-regulation of cell division cycle (cdc) 2 kinase in neurons with early stage Alzheimer's disease neurofibrillary degeneration

The major component of Alzheimer's disease (AD) neurofibrillary tangles (NFTs) is abnormally hyperphosphorylated tau aggregated as paired helical filaments (PHFs). Cell division cycle (cdc) 2 kinase is one of the main candidate kinases that phosphorylates normal tau in vitro at several sites seen in PHF-tau. Using brains staged according to Braak and Braak criteria, we investigated the role of cdc2 in neurofibrillary changes in the hippocampal formation, and the entorhinal and temporal cortices. Neurons with tangle-like inclusions positive for active cdc2 were found to appear first in the Pre-alpha layer of the entorhinal cortex, and then extend to other brain regions co-incident with the progressive sequence of neurofibrillary changes. This predictable progressive pattern is not associated with amyloid. The intraneuronal accumulation of active cdc2 appeared to precede the deposition of PHF-tau phosphorylated at Ser 202/Thr 205 sites. These data are consistent with the notion that cdc2 might be involved in the abnormal hyperphosphorylation of tau and consequently aggregation of tau into PHF at an early stage and that increased cdc2 activity is not consequent to the deposition of beta-amyloid in AD brain.     

延期手术对新生儿重症膈疝疗效的初步探讨

目的　探讨延期手术对新生儿重症膈疝的疗效和意义。方法　本文回顾 1985～ 2 0 0 3年复旦大学附属儿科医院收治的 2 0例重症新生儿先天性膈疝 (CDH)病例 ,其中 11例患儿进行了延期手术 ,最短 12h ,最长 5 4h ,平均2 5 3h ,3例围术期吸入一氧化氮 (NO)。以急诊手术 9例作为对照 ,对一般情况、围术期动脉血气、超声心动图结果、手术疗效和预后随访进行对照分析。结果　急诊手术组入院pH(7 19± 0 0 3)、PCO2 (18 4 3± 1 0 5 )kPa ,术后pH(7 2 4± 0 0 4 )、PCO2 (9 0 4± 4 6 6 )kPa ,术后平均呼吸机使用时间 4 0 7d ,其中 4例存活 ,5例死亡 ;延期手术组入院pH((7 19± 0 5 9)、PCO2 (6 89± 1 12 )kPa ,术前pH(7 37± 0 15 )、PCO2 (5 4 8± 2 2 6 )kPa ,都较入院有明显改善 ,术后pH(7 4 5± 0 0 2 )、PCO2 (5 0 5± 2 13)kPa ,恢复较急诊组理想 ,患者平均术后呼吸机使用时间 4 5 7d。延期组中患儿 10例存活 ,1例因术后严重肺炎无法脱离呼吸机 ,家属放弃治疗。结论　延期手术以及NO吸入等措施对于改善围手术期管理和稳定术前血流动力学指标及内环境有一定的作用 ,对降低新生儿先天性膈疝的病死率有积极的意义。     

Study on Remodeling of Astrocytes in Facial Neuclus after Peripheral Injury

To observe the glial reactions surrounding facial motor neurons following facial nerve anastomosis. At 1,7,21 and 60 d following facial nerve anastomosis, the recovery process of facial movement was observed, the glial fibrillary acidic protein （GFAP） immunoreactivity was analyzed by a combined method of fluorescent retrograde tracing and immunofluorescent histochemical staining, and the uhrastructure of astrocytes were observed under a transmission electron microscope （TEM）, respectively. Postoperatively the function of facial muscles could not return to normal, often accompanied with hyperkinetic syndromes such as synkinesis at the late stage. Motor neurons in every facial subnucleus could be retrogradely labeled by fluoro-gold （FG）, and displayed an evident somatotopic organization. Normally there was a considerable number of GFAP-positive cells in nonnucleus regions but few inside the facial nucleus region. Postoperatively the GFAP immunoreactivity in the anastomotic side increased significantly, but gradually decreased at the late stage. The ultrastructure of astrocytes in our experiment showed that the sheet-like process of astrocytes invested and protected the injured facial motor neurons. The present study shows that reactive astrocytes undergo some characteristic changes during the process of facial nerve injury and regeneration. The plastic change at the late stage may be involved in the mechanism of synkinesis.     

A Survey on Mechanisms of the Anti-aging Effects of Acupuncture and Moxibustion

TCM holds that the growth, development and aging of human beings are closely related with the state of the zang-fu organs and with the exuberance or depletion of qi and blood.     

恬尔心与西地兰静注治疗阵发性房颤过快心室率疗效对比观察

目的 观察静脉注射恬尔心与西地兰控制阵发性心房颤动心室率过快的效果。方法 将患者64例随机分为A、B两组各32例；A组恬尔心10mg加入0．9％NS10mliv st，B组西地兰0．4mg加入10％GS10mliv st，均在5min内注完，观察记录2—4h内HR、BP等变化情况。结果 两组治疗后心室率均降至正常，与治疗前比较p〈0．01：A组起效迅速，最大效应时间（8±2）min，与B组（52±24）min比较p〈0．01（t=10．335）。结论 恬尔心与西地兰对阵发性房颤心室率过速均有显著疗效，前者起效时间较后者迅速，但恬尔心不宜用于心衰的患者。     

丙谷胺对ERCP术后胰腺功能影响的评价

目的探导丙谷胺对逆行胰胆管造影(ERCP)术后胰腺功能的影响。方法比较对照组(S组)、奥曲肽组(O组)、丙谷胺组(P组)急性胰腺炎的发生率、死亡率、血浆胆囊收缩素八肽(CCK-OP)及血清淀粉酶含量的变化。结果S组并发急性胰腺炎3例(13.6%,3/22),O组并发急性胰腺炎1例(5%,1/20),P组并发急性胰腺炎1例(4.0%,1/25),S组明显高于O组及P组(P<0.05)。术后6h及24hP组及O组血清淀粉酶与S组比较差异均有显著性(P<0.05),但P组与O组比较差异无显著性(P>0.05)。术后6hP组血浆CCK-OP明显低于O组及S组,差异有显著性(P<0.05)。术后24hP组与O组及S组比较差异均无显著性(P>0.05)。结论丙谷胺可抑制CCK-OP的作用,与奥曲肽一样能有效地保护ERCP术后胰腺的功能,减轻酶血症,预防急性胰腺炎的发生。     

单项医疗设备经济效益分析方法初探

医疗设备的经济效益情况是医院经济管理的重要论题.单项医疗设备经济效益分析方法的研究对于提高医疗设备使用效率,降低运行成本具有实际意义.在这里,我们以我院医疗设备使用和管理的实际情况为背景,以简单可行作为出发点,总结一套单项医疗设备经济效益分析方法的研究方案,并从工作基础和条件、研究内容和途径、预期目标和工作步骤几个方面解析论述,力求找到一条简明的数据统计和分析的思路,希望在实际运用和理论分析的反复过程中,能够不断完善医疗设备经济效益分析方法的实用性和科学性,使之更有效应用于实际工作.     

三妙汤加味外洗治疗尖锐湿疣64例观察

目的:观察三妙汤加味外洗治疗尖锐湿疣的疗效。方法:治疗组64例用三妙汤外洗、对照组64例用疣必治外涂,均治疗1周。结果:总有效率治疗组96.88%、对照组为95.31%,两组比较无明显差异(P>0.05)。半年复发率治疗组10.41%、对照组32.00%,两组比较有显著性差异(P<0.05)。结论:三妙汤加味外洗治疗尖锐湿疣疗效好、复发率低。     

高聚生联合顺铂心包腔内灌注治疗肺癌致心包液的临床观察

Objective： To evaluate the therapeutic efficacy of injecting highly agglutinative staphylococcin （HASL） and cisplatin into pericardial cavity of lung cancer patients with pericardial effusion. Methods： 81 patients were randomized into two groups： 45 in the experimental group （HASL and Cisplatin） and 36 in the control group （Cisplatin）. At first pericardial effusion was drained out from a intrapericardial catheter and then different drugs were infused, respectively. 24 h after perfusion the draining continued again until drainage quantity was less than 30 mL every day. The draining lasted 10-15 days. Results： The response rate was 91.1% for the experimental group and 80.6% for the control group. There was no significant difference between the two groups （P〉0.05）. The complete remission was 77.8% for the experimental group and 52.8% for the control group, which was statistically significant difference （P〈0.05）. The adverse effects were myelosuppression and nausea and vomiting, which were 35.6% and 40.0% in the experimental group and 72.2% and 66.7% in the control group, respectively （P〈0.01, P〈0.05）. Conclusion： inject- ing HASL and cisplatin into pericardial cavity may be a better way to control pericardial effusion of lung cancer.