全文获取类型
收费全文 | 3511篇 |
免费 | 235篇 |
国内免费 | 23篇 |
专业分类
耳鼻咽喉 | 20篇 |
儿科学 | 132篇 |
妇产科学 | 32篇 |
基础医学 | 516篇 |
口腔科学 | 152篇 |
临床医学 | 322篇 |
内科学 | 879篇 |
皮肤病学 | 85篇 |
神经病学 | 272篇 |
特种医学 | 306篇 |
外科学 | 325篇 |
综合类 | 52篇 |
一般理论 | 1篇 |
预防医学 | 237篇 |
眼科学 | 37篇 |
药学 | 184篇 |
1篇 | |
中国医学 | 4篇 |
肿瘤学 | 212篇 |
出版年
2023年 | 14篇 |
2022年 | 22篇 |
2021年 | 55篇 |
2020年 | 31篇 |
2019年 | 43篇 |
2018年 | 46篇 |
2017年 | 38篇 |
2016年 | 46篇 |
2015年 | 95篇 |
2014年 | 72篇 |
2013年 | 116篇 |
2012年 | 158篇 |
2011年 | 158篇 |
2010年 | 121篇 |
2009年 | 122篇 |
2008年 | 161篇 |
2007年 | 175篇 |
2006年 | 173篇 |
2005年 | 175篇 |
2004年 | 184篇 |
2003年 | 180篇 |
2002年 | 121篇 |
2001年 | 98篇 |
2000年 | 61篇 |
1999年 | 48篇 |
1998年 | 114篇 |
1997年 | 99篇 |
1996年 | 70篇 |
1995年 | 70篇 |
1994年 | 52篇 |
1993年 | 54篇 |
1992年 | 52篇 |
1991年 | 52篇 |
1990年 | 42篇 |
1989年 | 71篇 |
1988年 | 62篇 |
1987年 | 56篇 |
1986年 | 46篇 |
1985年 | 50篇 |
1984年 | 36篇 |
1983年 | 34篇 |
1982年 | 22篇 |
1981年 | 28篇 |
1980年 | 29篇 |
1979年 | 28篇 |
1978年 | 27篇 |
1977年 | 22篇 |
1976年 | 23篇 |
1975年 | 18篇 |
1969年 | 13篇 |
排序方式: 共有3769条查询结果,搜索用时 0 毫秒
51.
Detecting pre-ovulatory luteinizing hormone surges in urine 总被引:2,自引:1,他引:2
Kesner JS; Knecht EA; Krieg EF Jr; Wilcox AJ; O'Connor JF 《Human reproduction (Oxford, England)》1998,13(1):15-21
The study objectives were to determine (i) if pre-ovulatory luteinizing
hormone (LH) surges, undetected in urine by two immunoradiometric assays
(IRMA), were detectable by an ultrasensitive immunofluorometric assay
(IFMA) and (ii) the influence of creatinine adjustment on the detection and
timing of the urinary LH surges. Daily urine specimens were contributed by
healthy 25-36 year old volunteers during 14 ovulatory menstrual cycles for
an epidemiological study conducted in 1983-1985. Specimens were selected as
having been previously assayed by two IRMA without consistently detecting
LH surges. These urine specimens were remeasured using an IFMA and adjusted
for creatinine concentration. IFMA measurements revealed unambiguous LH
surges in all cycles. Adjusting IRMA urinary LH values for creatinine
concentrations revealed previously undetected LH surges in four of eight
cycles. Creatinine adjustment also altered the timing of IRMA and IFMA LH
surges by 1-5 days. These results demonstrate an IFMA that detects pre-
ovulatory LH surges in unpreserved, frozen urine from cycles where such
surges were previously undetectable. Further, creatinine adjustment can
markedly affect detection and timing of the onset and peak of the urinary
LH surge. While our analysis suggests that this adjustment improves the
validity of the LH measure, this requires further investigation.
相似文献
52.
53.
54.
Children born into allergic families, with two allergic parents, are at high risk of developing allergy within the first 5 years of life. In order to observe possible external factors in the sensitization process, a prospective study of 13 such children was done, in which serial clinical and immunologic observations were made at 3- to 6-month intervals over a period of 1 to 4 yr. Eleven of these children are now clinically allergic; 5 have asthma. Immunologic evidence for allergic sensitization was observed in these 11 children by RAST, antigen-induced leukocyte histamine release, lymphoblastogenesis, and rise in serum IgE. Upper respiratory infections (URI) occurred in these 11 allergic children 1 to 2 months prior to the onset of allergic sensitization. In 10 of these 11 URI children, complement-fixing antibodies to viruses (parainfluenza, RSV, CMV) increased in the same blood samples in which immunologic allergic sensitization was first evidenced. This coincidence suggests that certain viruses may contribute to the allergic sensitization process. 相似文献
55.
Bloom's syndrome. XIX. Cytogenetic and population evidence for genetic heterogeneity 总被引:1,自引:0,他引:1
Cells with abnormally high rates of sister-chromatid exchange (SCE) are uniquely characteristic of Bloom's syndrome (BS). However, in one in five persons a minor population of cells with a low-SCE phenotype circulates in the blood. The origin and significance of the low-SCE cells in BS have never been understood, although they are assumed to arise by somatic mutation. In the present investigation, the enigmatic high-SCE/low-SCE mosaicism was investigated by comparing the incidence in several subpopulations of persons in the Bloom's Syndrome Registry who exhibit the two types of cells, and a striking negative correlation emerged: in persons with BS whose parents share a common ancestor, the case in approximately half of registered persons, low-SCE cells are found only rarely; conversely, the mosaicism occurs almost exclusively in persons with BS whose parents are not known to share a common ancestor. Because those who share a common ancestor are predominantly homozygous-by-descent at the mutated BS locus, the negative correlation is interpreted to mean that the emergence of low-SCE cells in BS in some way depends on the pre-existence of compound heterozygosity. A corollary to this is that BS is genetically heterogeneous. 相似文献
56.
Retrospective multicentre study on mechanical and enzymatic preparation of fresh and cryopreserved testicular biopsies 总被引:1,自引:0,他引:1
Baukloh V;German Society for Human Reproductive Biology 《Human reproduction (Oxford, England)》2002,17(7):1788-1794
BACKGROUND: Isolation of sperm suitable for ICSI from fresh or frozen-thawed testicular sperm extraction (TESE) can be facilitated by mechanical or enzymatic processing of the samples. METHODS: A retrospective multicentre study was initiated to compare these two approaches. Eleven German centres provided data on their TESE cycles performed during the period 1996/1997. Quality of retrieved sperm, fertilization rates of injected oocytes, embryo quality, resulting pregnancy rates and evolution of pregnancies were evaluated. RESULTS: The percentage of cycles with at least some motile sperm available for injection was higher after mechanical preparation. Independent of the preparation method, fertilization rates were higher for motile compared with immotile sperm or elongated spermatids in all groups and in general higher for cryopreserved versus fresh samples. Embryo quality was significantly better after injection of motile sperm for all treatments and in particular after enzymatic versus mechanical processing of biopsies. Pregnancy rates were identical for embryos derived from sperm prepared mechanically or enzymatically from fresh or cryopreserved testicular samples. The abortion rate (32/172, 18.6%) and the rate of multiple implantations (32/140, 22.9%) were not different from results reported in the literature for ICSI using ejaculated sperm. CONCLUSION: In this retrospective multicentre study, no unequivocal advantage of one over the other preparation method could be identified in 839 ICSI cycles using testicular sperm from 549 patients. 相似文献
57.
The Bloom's Syndrome Registry was published in this journal in 1977. Now, in the first in a series of progress reports, recent accessions to the Registry are recorded, new instances of neoplasia are listed, and recent clinical observations and experimental results of general interest are cited. 相似文献
58.
Lei Li Christine Eng Robert J. Desnick James German Nathan A. Ellis 《Molecular genetics and metabolism》1998,64(4):286-290
Bloom syndrome is more common in individuals of Ashkenazi Jewish descent than in any other population, and one particular mutation in the Bloom syndrome gene,blmAsh,is homozygous in nearly all Ashkenazi Jewish persons with Bloom syndrome. We have determined the frequency ofblmAshin 1491 Ashkenazi Jewish persons with no known history of Bloom syndrome and found that 1 in 107 persons was heterozygous. Although not common, genetic screening for Bloom syndrome is feasible in this population. 相似文献
59.
Schulz R Mittelmark M Kronmal R Polak JF Hirsch CH German P Bookwala J 《Journal of aging and health》1994,6(4):419-447
Baseline data on the perceived health status of participants (N = 5,201) in the Cardiovascular Health Study of the Elderly (CHS) are reported. The authors examined the predictive utility of health-related factors representing eight different domains, assessed gender differences in the prediction of perceived health, and tested a hypothesis regarding the role of known clinical conditions versus subclinical disease in predicting perceived health. Multivariate analyses showed that the majority of the explained variance in self-assessed health is accounted for by variables that fall into four general categories. Although gender differences were small, the analysis showed that the relative importance of several predictor variables did vary by gender. 相似文献
60.
J L Donovan J R Bell S Kasim-Karakas J B German R L Walzem R J Hansen A L Waterhouse 《The Journal of nutrition》1999,129(9):1662-1668
Flavonoids are components of fruits, vegetables and wines. An abundance of flavonoids in the diet is correlated with reduced heart disease mortality, suggesting that they act as protective nutrients. However, little is known about the absorption and metabolism of flavonoids after normal foods are consumed. This study measured the levels of one abundant flavonoid, (+)-catechin, and its metabolites in plasma after five male and four female volunteers consumed 120 mL of red wine (RW) one day and de-alcoholized red wine (DRW) on a separate day. Each wine sample contained 35 +/- 1 mg catechin (mean +/- SEM). Plasma levels of catechin and its metabolite 3'-O-methylcatechin (3'MC) were determined by gas chromatography-mass spectrometry (GC-MS) of the trimethylsilylated (TMS) derivatives. Glucuronide and sulfate conjugates were determined after enzymatic hydrolysis. Before RW or DRW consumption, plasma levels of catechin, 3'MC and all conjugates were <2 nmol/L. After 1 h, average levels of catechin, 3'MC and all conjugates increased to 91 +/- 14 nmol/L (RW) and 81 +/- 11 nmol/L (DRW). At 1 h, 21 +/- 1% of the metabolites were methylated and <2% of catechin and 3'MC were unconjugated. Catechin was present as both a sulfate conjugate and a conjugate containing both glucuronide and sulfate residues. 3'MC was present primarily as a glucuronide conjugate. At every time point, catechin was present almost exclusively as metabolites, and these levels were independent of ethanol. Therefore, if flavonoids are protective nutrients, the active forms are likely to be metabolites, which are far more abundant in plasma than the forms that exist in foods. 相似文献