EMMPRIN和MMP-2的表达与大肠癌临床病理学的关系及意义

目的:探讨大肠癌组织EMMPRIN和MMP-2蛋白的表达意义.方法:大肠癌患者108例,以正常结、大肠黏膜30例作为对照,应用SABC免疫组化方法检测EMMPRIN和MMP-2蛋白的表达.结果:大肠癌组织EMMPRIN和MMP2蛋白的阳性表达率分别为81.5%(88/108)和86.1%(93/108),明显高于正常大肠黏膜组织(6.7%和20.0%,P<0.01).EMMPRIN和MMP2在中、低分化癌中的强阳性表达率高于高分化癌(P<0.05);浆膜浸润及淋巴结转移组的强阳性率高于无浸润及淋巴结转移组(P<0.01);Dukes C-D期的强阳性表达率高于Dukes A-B期(P<0.01).EMM-PRIN和MMP2的表达一致率为91.4%(85/93),Spearman等级相关性检验分析表明大肠癌组织中EMMPRIN的表达和MMP2的表达之间呈正相关(r=0.608,P<0.01).结论:EMMPRIN和MMP-2与大肠癌的发生、浸润、转移有关,可作为新的大肠癌标记物用于联合检测,具有重要的临床意义.     

HPLC法测定清感穿心莲片中穿心莲内酯和脱水穿心莲内酯的含量

【目的】建立清感穿心莲片中穿心莲内酯和脱水穿心莲内酯的含量测定方法。【方法】采用高效液相色谱法:色谱柱为资生堂CAPCELL PAK C18柱(4.6 mm×250 mm,5μm);流动相:甲醇∶水(52∶48);检测波长:254 nm;流速:1.0 ml/min;柱温:40℃。【结果】穿心莲内酯在0.2~3μg(r=0.9999)、脱水穿心莲内酯在0.2~3μg(r=0.9999)范围内线性关系良好。穿心莲内酯和脱水穿心莲内酯的平均回收率分别为99.45%(RSD=0.19%,n=6),99.57%(RSD=0.19%,n=6)。【结论】该方法简便、准确、重复性好,可用于该制剂中有效成分的含量测定。     

ProTaper镍钛根管锉在根管预备中的应用体会

根管治疗术是目前临床上治疗牙髓炎最成熟的方法，ProTaper镍钛根管锉因其具有弹性好，切割力强，对根尖刺激小，术后急性反应小而倍受口腔医生青睐。我们就两年来临床上使用ProTaper镍钛根管锉与K型锉预备根管后的反应比较，现报告如下。     

2003-2007年重症监护病房革兰阴性杆菌的变迁与耐药性监测

目的 调查近5年重症监护病房(ICU)革兰阴性杆菌分离率及对常用抗菌药物的耐药性变化趋势,以指导临床合理使用抗菌药物.方法 分析2003年1月-2007年12月ICU分离出的革兰阴性杆菌,采用纸片扩散法进行药敏检测,并用WHONET5.3软件进行数据分析.结果 650株革兰阴性杆菌中占前3位的为不动杆菌属39.1%、铜绿假单胞菌20.9%及大肠埃希菌14.7%;不动杆菌属对亚胺培南、头孢哌酮/舒巴坦和哌拉西林/他唑巴坦的耐药率最低,分别为3.7%、28.3%和42.9%;近2年铜绿假单胞菌对亚胺培南的耐药率明显升高(58.0%);除嗜麦芽寡养单胞菌外,亚胺培南对其他革兰阴性杆菌保持强大的抗菌活性,嗜麦芽寡养单胞菌对头孢他啶的耐药率仍最低19.4%;革兰阴性杆菌对头孢哌酮/舒巴坦与哌拉西林/他唑巴坦的耐药率＜50.0%,对其他抗菌药物高度耐药,5年中耐药率无明显变化.结论 非发酵菌在ICU革兰阴性杆菌中占有较高比例,亚胺培南(除嗜麦芽寡养单胞菌外)、头孢哌酮/舒巴坦与哌拉西林/他唑巴坦对革兰阴性杆菌具有很好的敏感性,对其他抗菌药物耐药率较高.     

某高校大学新生艾滋病防治知识健康教育效果评价

目的探索适合大学生艾滋病防治的健康教育模式及途径,为有效预防和控制艾滋病在高校的流行提供依据。方法随机选取某高校选修和未选修健康教育课新生各900人为实验组和对照组,比较干预前后大学新生艾滋病相关知识、态度及行为状况。结果实验组AIDS相关知识知晓率从干预前的71.7%提高到干预后1个月、8个月的95.0%和95.5%,干预前、后及与对照组(80.6%)相比,差异均有统计学意义(P值均<0.01);正向态度率从干预前52.2%提高到干预后1个月、8个月的66.4%和67.7%,干预前、后及与对照组(57.7%)相比,差异均有统计学意义(P值均<0.01);正向行为率从干预前70.0%提高到干预后1个月、8个月的80.0%和80.2%,干预前、后及与对照组(71.5%)相比,差异均有统计学意义(P值均<0.01)。结论高校艾滋病防治应以大一新生为重点,强调性行为、性道德教育,专题授课、播放VCD、图片展等健康教育模式值得推广。     

3.0T磁共振SPACE技术在脊柱侧弯中的应用

目的:探讨3.0TMRI SPACE技术在脊柱侧弯中的应用价值。方法:对21例普通X光平片显示脊柱侧弯的患者应用MRI SPACE扫描技术,采用3D抑脂自旋回波各项同性的T1、T2加权序列,完成冠状位扫描,沿着脊髓的中心进行曲面重建完成一幅完整的显示椎管内脊髓全貌图像。结果:21例均可在一幅图像上完整显示扭曲的脊柱及椎管内脊髓的全貌,了解脊柱侧弯的程度、椎体骨质异常及椎管内脊髓受压变形等改变。结论:磁共振SPACE技术的应用,大大提高了对脊柱畸形诊断的直观性和准确性,对手术治疗有极大的指导作用。     

二维电子伤票的制作和应用

目的：目的：解决指战员基本信息的存贮、携带和信息更新问题，方便信息采集。方法：采用二维图像技术信息存贮，以卡片形式携带。结果：在大规模演练中使用，效果满意。结论：解决了一维伤票无信息载体和信息更新的问题，它有利于野战医疗所或基地医院对伤员信息的获取。     

Estimates of the chromium(VI) reducing capacity in human body compartments as a mechanism for attenuating its potential toxicity and carcinogenicity

Estimates of the overall reducing capacity of hexavalent chromium(VI) in some human body compartments were made by relating the specific reducing activity of body fluids, cell populations or organs to their average volume, number, or weight. Although these data do not have absolute precision or universal applicability, they provide a rationale for predicting and interpreting the health effects of chromium(VI). The available evidence strongly indicates that chromium(VI) reduction in body fluids and long-lived non-target cells is expected to greatly attenuate its potential toxicity and genotoxicity, to imprint a threshold character to the carcinogenesis process, and to restrict the possible targets of its activity. For example, the chromium(VI) sequestering capacity of whole blood (187-234 mg per individual) and the reducing capacity of red blood cells (at least 93-128 mg) explain why this metal is not a systemic toxicant, except at very high doses, and also explain its lack of carcinogenicity at a distance from the portal of entry into the organism. Reduction by fluids in the digestive tract, e.g. by saliva (0.7-2.1 mg/day) and gastric juice (at least 84- 88 mg/day), and sequestration by intestinal bacteria (11-24 mg eliminated daily with feces) account for the poor intestinal absorption of chromium(VI). The chromium(VI) escaping reduction in the digestive tract will be detoxified in the blood of the portal vein system and then in the liver, having an overall reducing capacity of 3300 mg. These processes give reasons for the poor oral toxicity of chromium(VI) and its lack of carcinogenicity when introduced by the oral route or swallowed following reflux from the respiratory tract. In terminal airways chromium(VI) is reduced in the epithelial lining fluid (0.9-1.8 mg) and in pulmonary alveolar macrophages (136 mg). The peripheral lung parenchyma has an overall reducing capacity of 260 mg chromium(VI), with a slightly higher specific activity as compared to the bronchial tree. Therefore, even in the respiratory tract, which is the only consistent target of chromium(VI) carcinogenicity in humans (lung and sinonasal cavities), there are barriers hampering its carcinogenicity. These hurdles could be only overwhelmed under conditions of massive exposure by inhalation, as it occurred in certain work environments prior to the implementation of suitable industrial hygiene measures.      

同种移植排斥反应中T细胞间接识别途径作用的研究

目的　CD+ 4T细胞通过间接识别途径识别MHC Ⅰ类移植抗原而活化已在离体实验中得到证实 ,但体内实验尚未见报道。方法　bml小鼠 (H 2Kbm1∶Kbm1,Db,Ⅰ Ab)的皮肤片分别移植到①B6小鼠 (H 2 b∶Kb,Db,Ⅰ Ab,n =8) ;②B6CD / 8小鼠 (H 2 b∶Kb,Db,Ⅰ Ab,CD / 8,n =8) ;③用抗CD4 抗体处理的B6CD / 8小鼠体上 (n =8)。观察移植皮肤的生长时间 ;用流式细胞仪 (FACScan)观察各组小鼠内T细胞亚群的变化。各组数据用分组或配对t检验进行数据统计。结果　尽管CD / 8小鼠体内未发现CD+ 8T细胞存在 ,但很快排斥了bm1皮肤移植片 (2 1 9d± 1 9d) ;而用抗CD4 抗体处理的B6CD / 8小鼠却长时间接受bm1皮肤移植片 (>47 5d± 3 8d) ;用流式细胞仪发现CD+ 4T细胞明显增殖活化并被抗CD4 抗体抑制。结论　CD+ 4T细胞通过间接识别途径活化并导致同种相异MHC Ⅰ类移植物的排斥。     

生长激素治疗生长激素缺乏症时心脏结构和功能变化

目的评价用人重组生长激素（r－hGH）治疗原发性生长激素缺乏症患者时心脏结构和功能变化。方法对9例确诊为原发性生长激素缺乏患者用r－hGH治疗前后及对20例年龄、性别相匹配的正常青少年通过一维和二维心超检查。结果治疗后患儿的空间隔厚度、左室后壁厚度及心肌重量指数明显上升,与正常相比,仍有一定差异,但差异比治疗前明显缩小。结论生长激素缺乏患者心脏的结构已经受累,但功能尚未有影响,经r-hGH治疗后,心肌重量指数明显上升,提示r-hGH对改善生长激素缺乏患者心脏结构有一定作用。