蒲芩消炎片的质量标准研究

目的:建立蒲芩消炎片(黄芩,蒲公英,苦地丁等)的质量标准.方法:采用TLC法对蒲公英、苦地丁进行鉴别;用高效液相色谱法测定制剂中黄芩苷的含量.结果:TLC鉴别方法专属性强.黄芩苷对照品在0.060～0.660μg范围内呈线性关系,r=0.99991,加样回收率为97.29%,RSD为2.13%.含量限度黄芩苷为不少于9mg·片-1.本方法操作简便,结果准确,灵敏度高,重复性好.结论:本质量标准可有效地控制蒲芩消炎片的质量.     

竹沥胶囊与氨溴索对支气管炎患者祛痰作用的临床随机对照研究

目的比较口服竹沥胶囊与氨溴索对支气管炎患者祛痰治疗的临床疗效和安全性。方法 238例支气管炎的患者,按照随机数字表分为2组,实验组124例口服竹沥胶囊3粒/次,3次/d;对照组114例口服氨溴索片30 mg/次,3次/d。2组疗程均为14 d。结果实验组和对照组的临床控制率分别为54.0%和55.2%,总有效率分别为88.6%和87.5%。实验组痰液性质、痰量、咳痰难易程度的临床控制率分别为75.8%,83.8%和72.5%;总有效率分别为89.5%,93.5%和83.8%。对照组临床控制率分别为75.4%,79.8%和74.5%,临床总有效率分别为88.3%,92.2%和87.6%。2组比较无显著性差异(P均>0.05)。实验组与对照组的不良反应发生率分别为0.8%和8.7%,2组比较有显著性差异(P<0.05)。结论口服竹沥胶囊对支气管炎祛痰效果与氨溴索等效,安全性好于氨溴索。     

Pre-B cells and other possible precursor lymphoid cell lines derived from patients with X-linked agammaglobulinemia

A group of unique Epstein-Barr virus-containing cell lines was derived from the bone marrow of three patients with X-linked agammaglobulinemia. Efforts to obtain cell lines from the peripheral blood of these patients were uniformly unsuccessful. Immunofluorescence analyses as well as biosynthetic studies with [(35)S]methionine indicated unusual patterns of Ig synthesis in many of these bone marrow derived lines. Seven of the lines were of particular interest in that two produced no Ig of any type; two others showed no Ig by fluorescence but small amounts by [(35)S]methionine labeling; one expressed only cytoplasmic μ chains without any evidence of light chain synthesis, and two produced primarily μ chains with only slight amounts of light chains. One of the lines without membrane or cytoplasmic Ig studied in detail grew like a typical lymphoid line and was carried in intermittent culture over a period of 2 yr without Ig expression. One line grew quite differently and resembled the round cell type described previously, which has been obtained from a variety of sources. The cell line with cytoplasmic μ chains and no light-chain expression had the characteristic properties of pre-B cells. Three normal type Ig-producing cell lines also were obtained from the patients. The accumulated evidence obtained in the present study indicates that these unusual cell lines represent normal precursor cells of the B-cell lineage; these grew out in these cases because of the virtual absence of mature B cells that ordinarily overgrow the culture system. However, the possibility that in certain instances they reflect abnormal Ig synthesis characteristic of the disease has not been ruled out.     

五大连池矿泉单次浴对人体某些生理机能的影响

目的观察矿泉浴对人体生理机能的影响。方法对６０例患消化系统疾病和皮肤病患者，进行温浴（将冷矿泉水加热至３７℃左右）冷浴（天然矿泉水，水温５～８℃），分别于浴前、浴后即刻，浴后３０分钟检测皮温、血压和脑血流图。结果皮温：温浴略有升高；冷浴即刻下降，３０分钟后升高明显（Ｐ＜０．０１）。血压：温浴下降；冷浴即刻有所上升，３０分钟后降压显著（Ｐ＜０．０５）。脑血流图：温浴和冷浴均无显著变化（Ｐ＞０．０５）。结论冷矿泉浴优于温矿泉浴     

Effects of corn stalk cultivation substrate on the growth of the slippery mushroom (Pholiota microspora)

Corn stalks are a major source of agricultural waste in China that have the potential for more efficient utilisation. In this study, we designed substrate formulas with different proportions of corn stalks to cultivate Pholiota microspora. The substrate formula for P. microspora cultivation that could partially or completely replace sawdust with corn stalks was selected through the analysis of mycelial growth rates, fruiting body traits, yield, biological efficiency, nutrients, and mineral composition. Our results showed that the substrate formula T2 (38% wood chips and 38% corn stalks) resulted in the highest yield of 275.66 ± 2.87 g per bag, which was 6.60% higher than that of formula CK, and the highest biological efficiency of 90.75 ± 0.04%, which was 4.58% higher than that of CK, with no significant differences from CK in terms of fruiting body traits, nutrients, or mineral composition. The substrate formula T1 (19% corn stalks) led to mushroom yields with the highest mineral and amino acid contents and was thus more suitable for the cultivation of medicinal P. microspora. Therefore, substrates comprising a mixture of corn stalks and sawdust can be used as a novel, inexpensive, and high-yield alternative for the cultivation of P. microspora.

Corn stalks are a major source of agricultural waste in China that have the potential for more efficient utilisation. In this study, we designed substrate formulas with different proportions of corn stalks to cultivate the Pholiota microspora.     

糖克煎剂对糖尿病大鼠肾组织TGF-β_1表达的影响

目的:观察并探讨具有祛痰化瘀作用的中药复方糖克煎剂对实验性糖尿病大鼠肾组织中转化生长因子β1(TGF-β1)表达的影响。方法:雄性Wistar大鼠78只,一组予普通饲料喂养,其余5组予高糖高脂饲料喂养,1个月后经尾静脉1次性注射链脲佐菌素(STZ)造成糖尿病及痰浊血瘀中医证候模型。应用具祛痰化瘀作用的中药复方糖克煎剂分别在造模成功及成模后4周灌胃给药,并设立洛汀新组为西药对照组。检测大鼠肾组织中TGF-β1mRNA的表达。结果:糖克煎剂可抑制模型大鼠肾皮质TGF-β1mRNA的基因表达,糖克预防组疗效优于糖克治疗组。结论:中药复方糖克煎剂能够预防和治疗大鼠早期糖尿病肾病,其机制可能与抑制组织中TGF-β1的过度表达有关。     

论医药卫生法学专业学生实务能力的培养

加强医药卫生法学专业学生实务能力的培养,是实现医药卫生法学专业培养目标的需要.在教学中,通过运用案例教学法、讨论式教学法,开展模拟法庭、第二课堂活动,组织临床见习、毕业实习等途径,培养即有法律知识又有医药卫生知识的复合型人才.     

经方药对研究与应用的思路与方法

研究与应用经方(即张仲景方)药对既不同于<中药学>所研究的单味药,也不同于<方剂学>所研究的固定方药,药对虽与<中药学>、<方剂学>有一定的潜在关系,但也有其本质不同.研究药对只有认清药对与<中药学>、<方剂学>所研究内容不同,才能认清药对对指导临床合理组方用药具有重要作用,才能认清药对在临床治疗中所起治疗作用举足轻重.又,研究与探索经方药对必须懂得药对既是经方组成的基本结构单元,又要认清药对是指导临证科学组方用药的理论依据及提高临床治疗效果的必备要素.     

论六经辨证

研究六经辨证主要包括六经生理、六经病理、六经病证与六经辨证。六经生理是探索六经辨证的物质基础;六经病理是研究六经生理异常变化;六经病证是研究六经所系脏腑及经脉气血阴阳病理变化在临床中的反映即症状、体征及脉象;六经辨证是根据六经病证而进行归纳、分析、总结、判断,以得出的诊断结论,六经辨证有本证辨证、兼证辨证及类似证辨证。     

原发性小肠肿瘤87例临床分析

目的 探讨原发性小肠肿瘤的诊断与治疗。方法 回顾性分析1993年6月至2004年7月诊治的87例原发性小肠肿瘤的资料。结果 原发性小肠肿瘤发病男女之比1．8：1，50～60岁为发病高峰，良、恶性肿瘤之比1：4．8。主要临床表现为腹痛、消化道出血、贫血、恶心、呕吐。30例十二指肠肿瘤中，20例（66．7％）术前确定病变部位；57例空回肠肿瘤中，17例（29．8％）术前确定病变部位。十二指肠肿瘤手术切除率76．7％（23／30），空回肠肿瘤手术切除率98．2％（56／57）。结论 小肠肿瘤术前确诊率仍较低，新的诊断方法尚未成为常规，手术切除是小肠肿瘤的最有效治疗。