7～12岁儿童铁缺乏及补充对身体做功能力的影响

以１１６０名７～１２岁儿童为研究对象，共筛查出ＩＤＡ儿童２６９人，贫血患病率为２３．１９％。抽取６０名ＩＤＡ儿童分为二组（补铁组、安慰剂组），另选３０名正常儿童为对照组。补铁前后测Ｈｂ、ＦＥＰ、ＰＷＣ１７０、ＲＰＷＣ１７０、ＶＯ２ｍａｘ及血乳酸浓度值。补铁前，ＩＤＡ儿童反映ＰＷＣ大小的各项指标值与正常儿童之间存在显著性差异；经三个月补铁后，补铁组以上各指标值恢复正常水平。结果提示：轻度ＩＤＡ对７～１２岁儿童ＰＷＣ有明显影响，并能通过铁剂治疗得以矫正。     

特异性DNA倍增技术检测t-PA cDNA基因在表达细胞中的稳定性

利用特异性DNA倍增技术(polymerase chain Reaction,PCR)检测t-PA cDNA基因在表达细胞基因组中的稳定性,并对所得的PCR反应产物进行了限制性内切酶片段、分子杂交和核苷酸顺序分析等方面的研究,证实了t-PA cDNA基因已插入到表达细胞染色体中。这种方法快速、简便、灵敏度和特异性高,是检测基因工程表达细胞中cDNA基因稳定整合状况的好方法。     

子宫颈癌手术前放疗28例临床分析

我院１９８９年至１９９４年共行子宫颈癌广泛切除术和盆腔淋巴结清扫术３９例，其中２８例行术前放射治疗，１９例临床诊断为Ⅱｂ，随着手术技术的提高和术前放射治疗的配合，子宫颈癌的手术适应症的选择，可以扩大至Ⅱｂ，不增加手术的并发症，并具有一定的优点。     

断腕再植术后手内在肌挛缩

探讨断腕再植术后手内在肌挛缩的发生率、治疗方法及预防。方法:作者总结了近24年内进行断腕再植并得到随访的63例资料,对其中发生手内在肌挛缩41例的发病机理、治疗结果及预防进行了分析和讨论。结果:63例断腕再植术后有41例发生手内在肌挛缩,发生率为65％。缺血时间超过16小时与16小时内的断腕再植术后手内在肌挛缩的发生率,两者差异有显著性意义(t=3.81,p<0.05)。二期行手内在肌手术矫正者效果不理想。结论:断腕再植术后手内在肌挛缩的处理关键在于预防。清创后用肝素化全血对离断手进行灌注,早期行手内在肌筋膜间区减压,将会取得较好的效果。     

硒和维生素C对白血病细胞增殖及细胞毒性的研究

目的本文主要研究硒和维生素Ｃ影响髓系与非髓系白血病细胞增殖及其细胞毒性的异同。方法体外培养髓系白血病细胞株ＨＬ６０及非髓系细胞株Ｋ５６２，培养时分别设Ｓｅ（５μｍ、８μｍ、１１μｍ）及ＶｉｔＣ（５μｍ、１０μｍ、１００μｍ）实验组及对照组，培养２４ｈ、４８ｈ、７２ｈ后分别取样，利用台盼蓝拒染法进行细胞计数，绘制细胞生长曲线，并通过噻唑蓝比色分析法研究Ｓｅ及ＶｉｔＣ对ＨＬ６０、Ｋ５６２细胞毒性。结果细胞生长曲线显示：Ｓｅ（５～１１μｍ）及ＶｉｔＣ（５～１０μｍ）对ＨＬ６０、Ｋ５６２细胞增殖均有抑制作用。ＭＴＴ结果显示：Ｓｅ（５～１５μｍ）对两种细胞均有细胞毒性。ＶｉｔＣ（５～１０μｍ）对ＨＬ６０具有细胞毒性，而ＶｉｔＣ（５～１００μｍ）对于Ｋ５６２，短时间内毒性作用较明显，随作用时间延长而减弱。结论本实验结果提示：Ｓｅ及ＶｉｔＣ对髓系和非髓系白血病细胞增殖均有抑制作用，但ＶｉｔＣ对于ＨＬ６０和Ｋ５６２作用机制是不同的。这为白血病的化学预防和临床辅助治疗提供一定的理论依据。     

Lesbianism in China

Lesbianism in China has a long but usually hidden history. This paper examines the historical and literary sources of the past to illustrate the history of lesbianism and then turns to a survey of lesbianism in the China of today. As in the past, lesbianism remains more or less hidden, and comes to light only occasionally. Data for contemporary China comes from a visit to an institution for delinquent young women, recent police records, and contemporary fiction. It has only been in the past 4 or 5 years that it was possible to talk about lesbianism and most lesbians are fearful of becoming identified.     

肾移植后细菌败血症治疗过程中内毒素血症和菌血症的动态变化

为研究败血症时细菌溶解的程度对内毒素血症的影响,本文对肾移植术后怀疑为革兰氏阴性杆菌败血症患者的血中内毒素和细菌进行了定量检查。13名患者中有4人血中细菌定量>50cfu/ml,其血中内毒素水平由开始检查到最高峰时相差2～35倍,有一名患者在使用抗生素治疗过程中突然发生休克,最后由于呼吸、循环衰竭而死亡,其血中内毒素明显升高。本文结果表明:对于感染发病率很高的肾移植患者,如何控制和处理抗生素治疗后的高内毒素血症是一个值得研究的问题。血中内毒素和细菌的定量检查可以为及时采取针对性措施,防治内毒素血症提供依据。     

聚甲氧基硅氧烷及其功能化产物研究Ⅱ.分子结构表征

通过正硅酸甲酯(TMOS)的水解缩合反应制备了聚甲氧基硅氧烷(PMOS)，其分子式可表示为：[SiOa(OH)b(OCH3)c]n。用丙烯酸-2-羟乙酯(HEA)进行酯交换后，制的功能化产物聚丙烯酰氧基甲基硅氧：烷(PAMOS)，其分子式可表示为：[SiOd(OH)c(OCH3)f(OCH2CH2OCOCH=CH2)g]k。通过二氧化硅分析、红外光谱(FTIR)、凝胶渗透色谱(GPC)等方法确定了分子式中a、b、c、n及d、e、f、g、k的值，是一种比较方便、快捷且准确的方法。     

Involvement of Cerulospinal Glutamatergic Neurotransmission in Fentanyl-induced Muscular Rigidity in the Rat

Background: Investigators in the authors' laboratory previously established the critical participation of the cerulospinal noradrenergic pathway in muscular rigidity elicited by fentanyl. The identification of colocalization of glutamate with tyrosine hydroxylase in most locus ceruleus neurons suggests a role for cerulospinal glutamatergic neurotransmission in fentanyl-induced muscular rigidity. This suggestion and the subtype(s) of glutamate receptors involved were investigated here.

Methods: Electromyographic signals activated by bilateral microinjection of 2.5 micro gram fentanyl into the locus ceruleus were recorded differentially from the left sacrococcygeus dorsi lateralis muscle of adult male Sprague-Dawley rats. The effect of intrathecal administration at the lower lumbar spinal cord of various N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists or agonists on this index of muscular rigidity was studied. Rats were under mechanical ventilation, and intravenous infusion of ketamine (30 mg [center dot] kg sup -1 [center dot] h sup -1) was maintained until 10 min before fentanyl was administered.

Results: Microinjection of fentanyl bilaterally into the locus ceruleus increased the root mean square and decreased the mean power frequency values of electromyographic signals. The efficacy of fentanyl to elicit muscular rigidity in this manner was significantly reduced by previous intrathecal administration of either 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK-801), D-(-)-2-amino-5-phosphonovaleric acid (AP5), or (+/- (CPP). Intrathecal administration of kainic acid or NMDA also resulted in significant electromyographic activation.