原子吸收光谱法测定糖尿病患者血清锌、镁、钙含量的研究

采用原子吸收光谱法测定了30例糖尿病患者血清锌、镁、钙的含量,并与30例正常人相比较。结果表明,其锌和镁较正常人为低(P<0.05),但钙与正常人无显著性差别。另外,本文还对锌、镁与糖尿病的关系进行了探讨。     

血管缺损断指的回植

某些断指的伤情复杂，常合并血管缺损，回植难度大。我们在微血管移植动物实验和尸体手指显微解剖的基础上，据情分别采用了１２种方法，修复断指中血管缺损共２０７例（２６１指），成活２４０指，成活率９２％。我们认为精湛的显微外科技术和正确选择处理方法，是手术成功的关键。     

EVALUATION OF INDOCYANINE GREEN RETENTION AND AMINOPYRINE BREATH TESTS IN PATIENTS WITH MALIGNANT BILIARY OBSTRUCTION

Impaired hepatic function is a major contributory factor to the high incidence of postoperative morbidity and mortality in patients with malignant biliary obstruction. Dynamic hepatic function tests such as indocyanine green (ICG) retention and aminopyrine breath tests were evaluated in such patients to define whether they were clinically useful for prediction of postoperative morbidity and mortality. Forty-four patients with malignant biliary obstruction undergoing surgery for relief of obstructive jaundice were recruited into the study. Indocyanine green retention and aminopyrine breath tests were carried out in all patients pre-operatively and repeated in 36 patients postoperatively. The ICG retention was abnormal in all patients before surgery and there was significant improvement 2 weeks after surgery (32.8 ± 2.5%vs 18.3 ± 2.8%, P= 0.001). The change in ICG retention levels correlated with the serum bilirubin levels but the pre-operative ICG retention value could not predict postoperative morbidity and mortality. The aminopyrine breath test was abnormal in all but one patient. It correlated with pre-operative prothrombin time of the patients before surgery but it did not improve significantly after surgery and was not predictive of postoperative outcome. It is concluded that both ICG retention and aminopyrine breath tests have limited clinical value in the pre-operative evaluation of patients with malignant biliary obstruction.     

脆性X综合征中不稳定的DNA序列和异常甲基化研究

采用ＰＣＲ结合序列胶分析的方法，对８２条正常中国人Ｘ染色体ＦＲＡＸＡ位点（ＣＧＧ）ｎ重复序列拷贝数的多态性进行了测定，其范围为２１～３１，高峰为２７。并通过Ｓｏｕｔｈｅｒｎ杂交分析了来自６个Ｆｒａ（Ｘ）家系的１５名成员（ＣＧＧ）ｎ的拷贝数与该重复序列上游ＣｐＧ岛的甲基化状态。Ｆｒａ（Ｘ）患者（ＣＧＧ）ｎ大量扩增并伴随ＣｐＧ岛异常甲基化。Ｆｒａ（Ｘ）携带者女性（ＣＧＧ）ｎ扩增较少，有嵌合现象。其子代或产生更大扩增，或保持原来状态，呈动态突变遗传特征。     

1998-2002年《中国防痨杂志》来稿与刊登情况分析

目的　分析中国防痨杂志来稿及刊登情况,进一步提高杂志质量,加快防痨学术交流。方法 以编辑部1998—2002年稿件登记本和1998—2003年出版的《中国防痨杂志》29册为样本,统计来稿和刊登稿件的数量以及出版时滞。结果 5年共来稿2592篇,年平均518.4篇,刊出759篇,平均刊登率为30.4%。但出版时滞稍长。结论 中国防痨杂志的稿源比较充足,但来稿质量仍需提高。出版时滞尚需改进。     

褪黑素对大鼠脑缺血再灌注损伤及胞浆型磷脂酶A_2蛋白表达的影响

目的观察褪黑素在脑损伤时对脑细胞凋亡和胞浆型磷脂酶A2(cPLA2)蛋白的表达及相关介质的影响。方法采用线栓法制作大脑中动脉缺血再灌注模型,将78只雄性Wister大鼠随机分为假手术组(6只)、模型组(36只)、治疗组(36只)。用HE染色和TUNEL法检测各组海马CA1区神经细胞凋亡;用免疫组织化学法检测海马CA1区神经细胞中cPLA2蛋白表达;用ELISA法检测血清中TNF-α和前列腺素E2(PGE2)水平。结果与假手术组比较,模型组不同时间点凋亡细胞及cPLA2蛋白表达增强,且血清中TNF-α和PGE2水平也显著增高(P<0.01)。与模型组比较,治疗组能明显降低海马CA1区细胞凋亡数和cPLA2蛋白表达,同时也能降低血清中TNF-α和PGE2水平(P<0.01)。结论褪黑素对大鼠大脑中动脉缺血再灌注脑损伤有良好的保护作用:降低缺血再灌注后血清中TNF-α和PGE2水平,抑制缺血再灌注后海马CA1区cPLA2蛋白表达。     

正常人在静态和运动状态下"死腔/潮气"估测值和实测值的比较

目的研究正常人静态及运动状态下死腔/潮气(VD/VT)估测值和实测值的关系.方法23名受试者行心肺运动试验,同步实时测定摄氧量和二氧化碳排出量,分别在运动前及运动高峰时抽取动脉血,根据Bohr的公式,得出VD/VT实测值,同时再用呼气末CO2分压(PetCO2)代替PaCO2,计算VD/VT,得出VD/VT估测值.结果根据病史、体检、肺功能和运动前后的心电图判断23名受试者均为正常受试者.静态时的VD/VT估测值和VD/VT实测值分别为0.359±0.109和0.354±O.106,两者无显著性差异(P=0.710),相关分析显示两者高度相关(r=0.911,P＜0.001).运动高峰时的VD/VT估测值和VD/VT实测值分别为0.234±0.070和0.248±0.094,两者无显著性差异(P=0.748),相关分析表明两者显著相关(r=0.783,P＜0.001).与静态时比较,运动时估测和实测VD/VT均明显下降.结论对于正常成人,无论是在静态或最大运动状态下,可用无创方法计算VD/VT代替VD/VT实测值.     

Conners教师评定量表的中国城市常模和信度研究

目的　建立Conners教师评定量表 (TRS)的中国城市常模 ,并对其进行信度检验。方法　在全国 14个城市对 35 76名儿童年进行Conners教师量表的评定 ,对其中 4 0名儿童间隔二周进行重测。结果　TRS各条目分与量表总评分之间的相关系数在 0 2 6 5～ 0 6 89;总问卷Cronbachα系数为 0 94 1,Spearman brown分半信度为 0 896 ,间隔二周量表总分的重测相关系数为 0 5 4 4 ,4个因子的重测相关系数在 0 391～ 0 6 71(P <0 0 1)。结论　Conners教师评定量表的中国城市常模符合我国国情 ,具有良好的信度 ,值得推广和应用。     

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