Mozart effect in preschool children?

In 1993, Rauscher et al. reported a temporary increase in spatial-temporal ability after listening to Mozart's music. This led to numerous replication and extension studies with mixed findings in the past decade. This study investigated the 'Mozart effect' in preschool children. Forty-one boys and girls, aged three to five, attempted a series of pencil-and-paper maze tests after each of three listening conditions: Mozart's Piano Concerto in A Major (K488), age-appropriate popular music and silence. Overall, there were no statistically significant differences among the three interventions. The results are discussed in relation to the findings of other investigations of the Mozart effect, and the need for further investigation of possible trends.     

重组腺相关病毒-阳离子脂质体载体的构建及评价

目的探讨重组腺病毒相关病毒（rAAV）-阳离子脂质体（LyoVec）复合载体的构建方法及其转染率评价。方法构建rAAv～GFP，rAAv—LyoVec复合载体及rAAV—GFP—LyoVec。倒置荧光显微镜观察，测定rAAV—GFP，LyoVec—GFP及rAAV—GFP-LyroVec对定量C6细胞的转染率，筛选出高转染率载体。结果rAAV—GFP组24、48、72和96h转染率分别为16％、23％、21％和9％；LyoVec-GFP组为28．5％、33％、32％和11％；rAAV—GFP—Lyovec组为49％、59％、64％和20％。rAAV-GFP—LyoVec纽分别在24、48、72和96h的转染率显著高于rAAV—GFP组（P〈0．01）及LyoVec—GFP组（P〈0．01）。结论新构建rAAV—LyoVec复合载体基因的转染效率远远高于rAAV或LyoVec独立转染。     

光子嫩肤仪配合激光治疗皮肤光老化疗效的观察

目的 探讨光子嫩肤仪配合755nm激光治疗皮肤光老化的疗效。方法 根据皮肤光老化者的肤质及疗效调节治疗方案，光子及激光交替进行治疗，每例治疗5次或5次以上。在治疗前和完成疗程后，由主治医师和接受治疗者共同对疗效做出评价。结果 本组178例，经治疗皮肤光老化症状均有不同程度改善，其中以肤色、毛细血管扩张、皮肤质地改善最为明显。临床观察表明，只要选择合适参数及完成疗程，治疗效果明显。结论 光子嫩肤仪配合755nm激光治疗皮肤光老化，是改善光老化表现的有效方法，并且比其他有损伤性治疗发生的合并症少，安全有效。     

凉粉草胶稀溶液的性质

研究了凉粉草胶多糖在20-40℃之间特性粘度对温度的敏感性，计算得其稀溶液的临界线团交叠值C^＊[η]平均为1．13，半稀释区斜率b为0．8～1．2，与其他多糖进行了比较。结果表明：凉粉草胶多糖的特性粘度在所测温度区域内的变化不敏感；凉粉草胶多糖在水溶液中，分子链构象可能为刚性杆状结构。     

牙冠延长术在残根残冠修复中的临床疗效

目的评价牙冠延长术在牙体缺损达龈下的残根残冠修复中的临床疗效。方法自2004年5月至2005年6月,对32例患者的36颗牙体缺损达龈下的患牙,术前按断端位于龈下最深距离分为A组(20颗患牙,距离<3.0mm)和B组(16颗患牙,3.0mm≤距离≤4.0mm),均采用牙冠延长术暴露龈下牙体断面,术后6周行桩冠修复。分别记录和比较手术前、后牙周探诊深度(PD)、菌斑指数(PLI)、龈沟出血指数(SBI)和牙齿松动度,以观察临床疗效。结果术后随访患者6个月,两组病例术后6周牙体断端均暴露较好、龈缘颜色正常,术后修复效果有效率为100%,满意率为77.78%。A组术后无松动,修复效果满意;B组术后50.00%出现Ⅰ度松动,修复效果欠佳,两组修复效果比较差异有显著意义(P<0.05)。术后各阶段两组的PD、PLI、SBI均明显优于术前(P<0.05),但两组术后牙周指数比较差异无显著意义(P>0.05)。结论牙冠延长术有利于残根残冠的保存和修复,但要注意适应证的选择。     

游离腓骨皮瓣移植Ⅰ期修复胫骨创伤性骨髓炎骨皮缺损

[目的]探讨游离腓骨皮瓣移植Ⅰ期修复胫骨创伤性骨髓炎合并骨皮缺损的方法和疗效。[方法]对156例胫骨创伤性慢性骨髓炎合并骨皮缺损患者，在行病灶清除的基础上行游离腓骨皮瓣移植，Ⅰ期修复骨皮缺损。[结果]经术后6—60个月的随访，皮瓣全部成活，发生静脉危象6例，再次吻合成功；伤口Ⅰ期愈合140例，Ⅱ期愈合13例，遗留窦道3例；骨髓炎复发2例。移植腓骨全部愈合，愈合时间2—6个月，平均3．2个月。[结论]慢性骨髓炎合并骨皮缺损可应用显微外科技术Ⅰ期修复。但是在感染的基础上进行手术，有一定的风险，严格掌握适应证。     

Evaluation of corticospinal tract injury with three-dimensional diffusion tensor tract in patients with acute cerebral infarction

Three-dimensional diffusion tensor tract (DTT) is the newest imaging to describe the structure of white matter fiber in three-dimensions, it has great significance in dividing the concrete anatomic site of gray and white matter lesions, displaying the correlation with fibrous band and judging clinical prognosis, which is incomparable by other imagings.OBJECTTVE: To observe the conditions of corticospinal tract (CST) in acute cerebral ischemic stroke patients,and analyze the relationship between motor function and the severity of CST injury.DESIGN: A case-control observation.SETTTNG: Department of Medical Imaging, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.PARTTCTPANTS: Fifteen patients with acute cerebral infarction were selected from Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from February to December in 2005. They all suffered from acute attack and motor disorder of hemiplegic limbs to different extent, and were conformed by CT or MRI.There were 9 males and 6 females, aging 16-87 years old, the median age was 51.7 years, and all were right handed. Fifteen right-handed normal subjects, who were matched by age and sex with the patients in the cerebral infarction group, were selected from the relatives of patients and physicians of the Imaging Department as the control group. All the subjects were informed and agreed with the study.METHODS: The patients with acute cerebral infarction and subjects in the control group received MR diffusion tensor imaging (DTI) with GE 1.5 T nuclear magnetic resonance system, fiber tracking with the software of dTV- Ⅱ. Fractional anisotropy (FA) maps and three-dimensional tractography of bilateral CST of all patients were created. Displacement, continuity and destroy of fibrous bands were observed. At the same time, muscle strength of ipsilateral hand of patients with cerebral infarction was measured with Brunnstrom standard. The correlation between the severity of CST injury and the muscle strength of ipsilateral hand was analyzed with spearman correlation analysis.MAIN OUTCOME MEASURES: ① FA values in the infarcted sites and those in the contralateral corresponding sites of patients with cerebral infarction; ② CST manifestations in the patients with cerebral infarction and the control group.RESULTS: All the 30 testees were involved in the analysis of results. ① The FA values in infarcted sites of white matter were significantly lower than those in the contralateral ones (t =4.570, P ＜ 0.001 ). ② In the control group, bilateral CST were reconstructed, they originated from precentral gyrus, went downwards to internal capsule, and extended to pontine and medulla oblongata, each fiber had good uniformity in continuous form. In the patients with cerebral infarction, the forms of contralateral CST were consistent with those in the control group with good continuity. Due to the involvement by the infarcted site to different extents, the ipsilateral CST manifested as continuous interruption and loss of uniformity in anatomic structure and form. The CST involvements were divided into three grades: integrated CST for grade I (n =2); integrated CST but compressed or displaced for grade 2 (n =5); interrupted CST for grade 3 (n =8). ③ The severity of CST injury was obviously correlated with the muscle strength of the ipsilateral hand (r=0.888, P＜ 0.05＝.CONCLUSION: ① CST is injured to different extents in patients with acute cerebral infarction, and the severity of injury is associated with muscle strength. It is indicated that it can be used to judge the prognosis of rehabilitative treatment. ②DTT can directly display the status of pyramidal tract more three-dimensionally.     

高转换型肾性骨病中骨保护素及其配体的表达和形态计量学观察

目的 观察高转换型肾性骨病中骨保护素及其配体 (OPG，RANKL)的表达，并与骨形态计量学指标进行相关分析。 方法 选择10例慢性肾衰尿毒症患者和3例正常人进行髂骨活检术，获得骨组织标本。采用免疫组化方法检测OPG和RANKL蛋白质的表达。采用全自动图像分析系统进行骨组织形态计量学测定。结果 10例慢性肾衰尿毒症患者经骨病理学检查证实均为高转换型骨病，以破骨细胞活化形成骨吸收陷窝伴或不伴骨矿化不全为特点。免疫组化显示尿毒症患者骨组织中以RANKL阳性表达为主。与正常对照相比，RANKL阳性表达细胞数目显著增加，OPG阳性表达细胞数目显著减少。尿毒症患者RANKL的阳性表达细胞数目与骨吸收面积和破骨细胞数目呈显著正相关。结论 高转换型肾性骨病中，PTH的溶骨作用可能是通过OPG/RANKL/RANK系统介导的。     

暴发流行的肺炎衣原体肺炎影像学表现分析

目的研究肺炎衣原体肺炎暴发流行的胸部影像学特征表现。方法对经过痰和咽试子标本,PCR,MIF检测证实的15例暴发流行的肺炎衣原体肺炎住院患者胸部X线和高分辨CT表现进行分析。结果本组暴发流行的肺炎衣原体肺炎患者均有发热,头痛,全身肌肉酸痛,干咳,声音嘶哑,咽痛等症状。肺部呼吸音减低或细湿啰音4例(26.7%),出现明显肺部影像学表现10例(66.7%)。10例暴发流行的肺炎衣原体肺炎常表现为多发或单发以小叶为中心阴影和腺泡状结节影(100%),病变以两中、下肺叶,外、中带分布;以小叶分布的气腔实变和磨玻璃样阴影(分别为100%和40%)和支气管血管束增厚(90%)。无肺门或纵隔淋巴结增大和胸腔积液。结论暴发流行的肺炎衣原体肺炎具有群体发病,临床和影像学表现有相似的特征,早期CT检查更能真实地反映病变大小、多少和分布范围。     

神经纤维瘤蛋白在先天性脊柱侧凸患者成骨细胞和软骨细胞中的表达

目的：检测神经纤维瘤蛋白在先天性脊柱侧凸患者成骨细胞和软骨细胞中的表达。方法：6例先天性脊柱侧凸患者，在后路手术时取髂骨及髂骨生长板，分离、培养成骨细胞和软骨细胞，分别行碱性磷酸酶染色和甲苯胺蓝染色。逆转录-多聚酶链反应（RT—PCR）检测神经纤维瘤蛋白mRNA．间接免疫荧光和Westemblot检测神经纤维瘤蛋白在成骨细胞和软骨细胞中的表达。结果：先天性脊柱侧凸患者成骨细胞和软骨细胞中存在Ⅱ型神经纤维瘤蛋白表达，该蛋白主要分布在细胞浆，所表达蛋白为三磷酸鸟苷酶活化蛋白（GAP）活性较弱的Ⅱ型异构体。结论：先天性脊柱侧凸患者成骨细胞和软骨细胞中存在神经纤维瘤蛋白表达，但该蛋白是否通过对成骨细胞和软骨细胞的影响导致骨骼系统异常还有待于进一步研究。