X连锁无汗性外胚叶发育不全家系ED1基因的突变检测

目的探讨国内X连锁无汗性外胚叶发育不全家系中ED1基因的突变情况，为该病的遗传咨询、产前诊断、确诊携带者提供依据。方法收集2个X连锁无汗性外胚叶发育不全家系及1个散发患者的外周血样本，盐析法提取基因组DNA，采用聚合酶链反应（polymerase chain reaction，PCR）和直接测序对ED1基因进行突变检测。结果家系一患者ED1基因第9外显子发生错义突变（1045G〉A），家系二和散发患者ED1基因第3外显子发生错义突变，分别为467G〉A和466C〉T。结论ED1基因的错义突变可导致X连锁无汗性外胚叶发育不全。这3个突变与国外学者的报道一致。     

口腔鳞癌癌周淋巴管生成与颈淋巴结微转移

目的　探讨口腔鳞癌癌周淋巴管生成与颈淋巴结微转移的关系。方法　对 4 7例口腔鳞癌组织、10例正常口腔黏膜进行酶组织化学染色 ,检测癌周微淋巴管密度 ;对 10例正常淋巴结、4 7例口腔鳞癌患者的 35 5个淋巴结以高分子量细胞角蛋白AE3单抗为抗体的免疫组织化学方法测定。结果　口腔鳞癌癌周淋巴管密度为 (14 0 4± 6 92 )个 / 2 0 0倍视野下 ,显著高于健康对照组 (5 4 8±2 6 2 )个 / 2 0 0倍视野下 (P <0 0 0 1) ;4 7例患者中角蛋白阳性 2 3例 ,阳性率为 4 8 9% (2 3/ 4 7) ;以淋巴微转移阳性、阴性分组 ,阳性组淋巴管密度为 (16 94± 5 4 3)个 / 2 0 0倍视野下 ,阴性组淋巴管密度为 (11 2 6± 5 0 0 )个 / 2 0 0倍视野下 ,阳性组微淋巴管密度显著高于阴性组 (P <0 0 0 1)。结论　癌周淋巴管密度增高在颈淋巴结微转移中起着非常重要的作用。     

根管预备并发症及充填质量的临床评价

目的：对根管预备并发症及充填质量进行临床评价。方法：从Digora图像系统中抽取我科2002—04—05所完成的根管治疗病例805例，分别对前牙、前磨牙和磨牙根管预备并发症的发生和充填质量进行分析。结果：形成台阶和侧穿的比例在前磨牙分别为4％，而在磨牙中则分别为33％和5％。前牙、前磨牙和磨牙适充的比例分别为74％、69％和50％。结论：磨牙根管预备和充填的质量仍有待进一步提高。     

口腔白斑、扁平苔藓细胞凋亡相关蛋白Bax表达的研究

目的 :探讨口腔白斑、扁平苔藓的癌变机理及发病机制。方法 :采用免疫组化法检测 10例正常口腔黏膜上皮 ,18例扁平苔藓 ,2 3例白斑 ,2 2例口腔鳞癌上皮组织中凋亡相关蛋白Bax的表达水平。结果 :白斑中上皮单纯增生、轻度、中度不典型增生和低分化鳞癌及糜烂型扁平苔藓的Bax蛋白显过度表达 ,与正常口腔黏膜上皮相比有显著性差异。结论 :Bax参与了口腔白斑癌变的早期过程 ,Bax的表达水平可作为临床监测白斑癌变倾向的参考指标。扁平苔藓的发病机制可能与细胞免疫亢进刺激Bax过度表达诱导角朊细胞凋亡有关 ,扁平苔藓的癌变机理有待进一步研究     

颈动脉手术围术期预测脑侧支循环的方法评价

To prediet the pateney of the cirele of Willis and cerebral ischemia during earotid artery surgery (ligation, resection, revascularization) is of rtmost importance both for the surgeons and the patients. Many methods have been proposed in the literature as simple and accurate means of evaluation the adequacy of cullateral hemispheric blood flow to compensate for a potentially resectable earoted artery. including Matas test, DSA, intraoperative measurement of stump pressure or back pressure, preoperative and intraoperative EEC monitoring, ocular plethysmograph (OPG),temporary balloon neelusion (TBO) alone or with single photon emission computerized tomography (SPECT). venon-enhanced computed tomography cerebral blood flow (Xe/CT CBF), and transcranial color Doppler (TCD). However, there is no single review and elinical studies showed that the combination of preoperative TBO and SPECT cerebral blood flow imaging significantly increases the safety of ICA/CCA resection, which can be used rontinely to predict the patients‘tolerance for earotid artery sacrifiee sithout developing neurologie deficits. Supported by Research Fund of the Ministry of Public Health (Grant No.96-1-340) and Sustentation Plan for Excellent Aeademic Leader of Shanghai Munieipality (Grant No.96XD14013).     

Acupuncture Anesthesia for Radiofrequency Catheter Ablation in Treatment of Persistent Atrial Fibrillation: A Case Report

Atrial fibrillation(AF)is a clinically common arrhythmia,which is prone to cerebral infarction and heart failure.Radiofrequency catheter ablation(RFCA)is currently considered the standard first-line treatment.During RFCA,cells are dehydrated and necrotized through heat,thereby achieving pulmonary electrical isolation;patients may experience pain and discomfort in the process.     

Identification of Boseongicola sediminum sp. nov., a Novel Decabromodiphenyl Ether (BDE-209)-tolerant Strain Isolated from Coastal Sediment in Xiamen,China

Polybrominated diphenyl ethers (PBDEs) are a group of aromatic organic bromine compounds, which have been used in a broad array of polymeric materials (plastics, foams, resins, and adhesives) as brominated flame retardants. They are used in commercial and household products, including textiles, electronic equipment, airplanes, and automobiles, especially in China and the USA, because they are inexpensive with excellent flame-retardant effects[1]. During the past decade, over 14,000 tonnes of PBDEs have entered the market, and have become ubiquitous environmental contaminants[2].     

急性大出血患者介入治疗的护理

笔报道急性大出血患介入治疗的护理，治疗前做好术前准备及局部麻醉的护理，术中密切配合术完成股动脉穿刺，保持呼吸道及输液通畅，监测生命体征等；术后观察穿刺点，穿刺侧肢体的皮肤颜色，足背动脉搏动及有无误栓重要脏器，做好相应的护理。     

联合术式合并异体肌腱重建圆韧带治疗小儿发育性髋关节脱位

目的探讨联合术式合并异体肌腱重建圆韧带治疗小儿发育性髋关节脱位(DDH)的手术方法及疗效。方法对48例小儿DDH患者(56髋)行软组织松解、股骨上段截骨、Salter或Pemberton髂骨截骨及异体肌腱移植、重建圆韧带术治疗,测定并对比手术前后的AI、CE角等指标,采用Severin影像学及McKay临床疗效评价标准评价疗效。结果 AI由术前36.2°~58.1°降低至11.4°~21.3°,CE角由术前-10°~-50°提高至12°~45°,差异均有统计学意义(P0.05)。患儿均获随访,时间1~4年。根据Severin X线评定标准:优30髋(53.6%),良21髋(37.5%),可5髋(8.9%)。根据McKay临床疗效评定标准:优29髋(51.8%),良20髋(35.7%),可4髋(7.1%),差3髋(5.4%)。结论联合术式结合异体肌腱重建圆韧带治疗DDH有利于提高成功率、减少并发症,但须强调联合化及个体化原则。     

膜生物反应器的设计及运行优化

膜生物反应器(MBR)是集生物降解和膜分离于一体的高效的新型水处理技术。膜生物反应器运行的经济性及处理废水的效率决定于膜生物反应器设计及运行参数的的合理性。文章从膜生物反应器的设计及运行参数优化方面予以综述。