全文获取类型
收费全文 | 433篇 |
免费 | 18篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 17篇 |
妇产科学 | 9篇 |
基础医学 | 54篇 |
口腔科学 | 6篇 |
临床医学 | 31篇 |
内科学 | 116篇 |
皮肤病学 | 7篇 |
神经病学 | 20篇 |
特种医学 | 10篇 |
外科学 | 19篇 |
综合类 | 4篇 |
预防医学 | 40篇 |
眼科学 | 10篇 |
药学 | 50篇 |
中国医学 | 2篇 |
肿瘤学 | 49篇 |
出版年
2024年 | 1篇 |
2023年 | 11篇 |
2022年 | 43篇 |
2021年 | 49篇 |
2020年 | 18篇 |
2019年 | 26篇 |
2018年 | 24篇 |
2017年 | 16篇 |
2016年 | 19篇 |
2015年 | 14篇 |
2014年 | 22篇 |
2013年 | 30篇 |
2012年 | 30篇 |
2011年 | 47篇 |
2010年 | 14篇 |
2009年 | 7篇 |
2008年 | 14篇 |
2007年 | 15篇 |
2006年 | 4篇 |
2005年 | 9篇 |
2004年 | 7篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有453条查询结果,搜索用时 15 毫秒
71.
Ewelina Jamrz Agnieszka Cabaj Lesaw Juszczak Joanna Tkaczewska Magorzata Zimowska Agnieszka Cholewa-Wjcik Pawe Krzyciak Pavel Kopel 《Materials》2021,14(22)
A novel, eco-friendly, and biocompatible method was applied to form silver nanoparticles (AgNPs) in great water dock (Lapathi radix) (KB) and pu-erh (Camellia sinensis) (PE) extracts. The surface plasma resonance peak of green synthesized AgNPs at 451.8 nm for AgNPs+KB and 440.8 nm for AgNPs+PE was observed via spectral analysis of UV absorbance. In this study, double-layered biopolymer films (FUR/CHIT+HGEL) with AgNPs incorporated in KB solution (AgNPs+KB) and AgNPs in PE solution (AgNPs+PE), were successfully prepared using the casting method. The SEM, XRD, zeta potential and size analyses confirmed the presence of AgNP in the films. The addition of AgNPs in plant extracts improved antimicrobial and antioxidant activity and thermal stability, whereas WVTR experienced a decrease. The nanocomposite films’ orange-brown colour may aid in the protection of food products against UV rays. The composite films demonstrated antibacterial activity against food-borne pathogens and may offer potential in food packaging applications. 相似文献
72.
Tarasiuk J Majewska E Seksek O Rogacka D Antonini I Garnier-Suillerot A Borowski E 《Biochemical pharmacology》2004,68(9):1815-1823
The appearance of multidrug resistance (MDR) of tumour cells to a wide array of antitumour drugs, structurally diverse and having different mechanisms of action, constitutes the major obstacle to the successful treatment of cancer. Our approach to search for non-cross resistant antitumour agents is based on the rational design of derivatives, which have a high kinetics of passive cellular uptake rendering their active efflux by MDR exporting pumps inefficient. Recently, two families of acridine cytotoxic agents were obtained, pyrazoloacridines (PACs) and pyrazolopyrimidoacridines (PPACs). The aim of this study was to examine molecular basis of the reported differences in retaining cytotoxic activity of these derivatives at cellular level against resistant erythroleukaemia K562/DOX (overexpressing P-glycoprotein) cell line. The study was performed using a spectrofluorometric method, which allows continuous monitoring of the uptake and efflux of fluorescent molecules by living cells. It was demonstrated that the presence of two additional rings, pyrazole and pyrimidine, fused to the acridine chromophore structure (PPAC) favoured more rapid cellular diffusion than the presence of only one additional pyrazole ring (PAC). The presence of hydrophobic substituent OCH3 markedly favoured the cellular uptake of pyrazoloacridines and pyrazolopyrimidoacridines while compounds having hydrophilic substituent OH exhibited very low kinetics of cellular uptake. In contrast, it was found that neither structure of the ring system nor the hydrophobic/hydrophilic character of examined substituents determined the rate of active efflux of these compounds by P-glycoprotein. Our data showed that a nearly linear relation exists between the resistance factor (RF) and lnV+ reflecting the impact of the cellular uptake rate (V+) on the ability of these compounds to overcome MDR. 相似文献
73.
Hanna Ławnicka Gabriela Mełeń-Mucha Ewelina Motylewska Sławomir Mucha Henryk Stępień 《Pharmacological reports : PR》2012,64(4):951-959
BackgroundThe risk of different cancers seems to be associated with obesity. Moreover, low ghrelin levels observed in obese people may be implicated in cancer development and progression. The aim of this study was to examine the direct effects of both forms of ghrelin (acylated and unacylated) and ghrelin receptor type 1a antagonist (D-Lys-GHRP-6) on the growth of murine colon cancer MC38 and human prostate cancer DU145 cell lines in vitro.MethodsThe cells were cultured for 72 h in the presence of rat or human acylated ghrelin (rG, hG), human unacylated ghrelin (hUAG), D-Lys-GHRP-6 (GHS-RA) applied either alone or jointly. The cell line growth was assessed by the colorimetric Mosmann method.ResultshUAG (10?6, 10?7 and 10?10 M) inhibited MC38 cancer cell growth and, at some concentrations (10?8, 10?9, 10?10 M), enhanced the antineoplastic effect of GHS-RA (10?4 M). In turn, GHS-RA evoked a biphasic effect on MC38 cancer growth: inhibitory at 10?4 M and stimulatory at 10?5 and 10?6 M. Moreover, GHS-RA at the highest examined concentration (10?4 M) enhanced the cytostatic effect of FU. Human acylated and unacylated ghrelin and GHS-RA inhibited DU145 cancer growth with moderate and different potencies. A dose-response effect was observed for the inhibitory action of hG together with the synergistic effect of hUAG and GHS-RA.ConclusionThe obtained results indicate an involvement of the ghrelin axis in the growth regulation of colon and prostate cancers and may suggest new therapeutic options for these neoplasms. 相似文献
74.
Matrix solid-phase dispersion (MSPD) is a simple and cheap sample preparation procedure allowing for the reduction of organic solvent consumption, exclusion of sample component degradation, improvement of extraction efficiency and selectivity, elimination of additional sample clean-up and pre-concentration step before chromatographic analysis. 相似文献
75.
76.
Ewelina Kowalewska Joshua B. Grubbs Marc N. Potenza Mateusz Gola Małgorzata Draps Shane W. Kraus 《Current Sexual Health Reports》2018,10(4):255-264
Purpose of Review
The current review summarizes the latest findings concerning neurobiological mechanisms of compulsive sexual behavior disorder (CSBD) and provides recommendations for future research specific to the diagnostic classification of the condition.Recent Findings
To date, most neuroimaging research on compulsive sexual behavior has provided evidence of overlapping mechanisms underlying compulsive sexual behavior and non-sexual addictions. Compulsive sexual behavior is associated with altered functioning in brain regions and networks implicated in sensitization, habituation, impulse dyscontrol, and reward processing in patterns like substance, gambling, and gaming addictions. Key brain regions linked to compulsive sexual behavior features include the frontal and temporal cortices, amygdala, and striatum, including the nucleus accumbens.Summary
Despite much neuroscience research finding many similarities between CSBD and substance and behavioral addictions, the World Health Organization included CSBD in the ICD-11 as an impulse-control disorder. Although previous research has helped to highlight some underlying mechanisms of the condition, additional investigations are needed to fully understand this phenomenon and resolve classification issues surrounding CSBD.77.
Klaus W. Lange Joachim Hauser Katharina M. Lange Ewelina Makulska-Gertruda Yukiko Nakamura Andreas Reissmann Yuko Sakaue Tomoyuki Takano Yoshihiro Takeuchi 《Current psychiatry reports》2017,19(2):8
Attention-deficit hyperactivity disorder (ADHD) is a common behavioral disorder in children and adolescents and may persist into adulthood. Insufficient nutritional supply of long-chain polyunsaturated fatty acids (LC-PUFAs) and other components including various minerals has been suggested to play a role in the development of ADHD symptoms. This review presents the evidence regarding the role of nutritional PUFA, zinc, iron, and magnesium supplements in the treatment of ADHD with a focus on the critical evaluation of the relevant literature published from 2014 to April 2016. The evaluation of therapeutic nutritional LC-PUFA supplementation in ADHD has shown mixed and inconclusive results and at best marginal beneficial effects. The benefits of PUFAs are much smaller than the effect sizes observed for traditional pharmacological treatments of ADHD. The effectiveness of PUFA supplements in reducing medication dosage has been suggested but needs to be confirmed. Zinc, iron, and magnesium supplementation may reduce ADHD symptoms in children with or at high risk of deficiencies in these minerals. However, convincing evidence in this regard is lacking. 相似文献
78.
79.
80.
Kiryk A Mochol G Filipkowski RK Wawrzyniak M Lioudyno V Knapska E Gorkiewicz T Balcerzyk M Leski S Leuven FV Lipp HP Wojcik DK Kaczmarek L 《Current Alzheimer research》2011,8(8):883-892
In the present study, we used a new training paradigm in the intelliCage automatic behavioral assessment system to investigate cognitive functions of the transgenic mice harboring London mutation of the human amyloid precursor protein (APP.V717I). Three groups of animals: 5-, 12- and 18-24-month old were subjected to both Water Maze training and the IntelliCage-based appetitive conditioning. The spatial memory deficit was observed in all three groups of transgenic mice in both behavioral paradigms. However, the APP mice were capable to learn normally when co-housed with the wild-type (WT) littermates, in contrast to clearly impaired learning observed when the transgenic mice were housed alone. Furthermore, in the transgenic mice kept in the Intellicage alone, the cognitive deficit of the young animals was modulated by the circadian rhythm, namely was prominent only during the active phase of the day. The novel approach to study the transgenic mice cognitive abilities presented in this paper offers new insight into cognitive dysfunctions of the Alzheimer's disease mouse model. 相似文献