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Jiwon Kim Spencer Krichevsky Lola Xie Maria Chiara Palumbo Sara Rodriguez-Diego Brian Yum Lillian Brouwer Richard T. Silver Andrew I. Schafer Ellen K. Ritchie Maria Mia Yabut Claudia Sosner Evelyn M. Horn Richard B. Devereux Joseph M. Scandura Jonathan W. Weinsaft 《Journal of the American Society of Echocardiography》2019,32(12):1574-1585
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Frank Daumann Robin Heinze Benedikt R?mmelt Anne Wunderlich 《Journal of urban health》2015,92(2):217-229
With an increasing percentage of the global population living in cities and the concurrent decrease in physical activity in daily life, public health issues for urban development have arisen. This study responds to that trend by presenting an approach to measure city-wide physical activity levels. Comparing of city indices for active sports and the active transportation shows differences between subject cities and activity level of age groups in sports as well as walking and cycling. Therefore, our study lends itself to implications for urban development towards creating a healthier city. 相似文献
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Reliability of tissue microarrays in detecting protein expression and gene amplification in breast cancer. 总被引:5,自引:0,他引:5
DaoHai Zhang Manuel Salto-Tellez Thomas Choudary Putti Elaine Do Evelyn Siew-Chuan Koay 《Modern pathology》2003,16(1):79-84
Tissue microarrays allow high throughput molecular profiling of diagnostic or predictive markers in cancer specimens and rapid validation of novel potential candidates identified from genomic and proteomic analyses in a large number of tumor samples. To validate the use of tissue microarray technology for all the main biomarkers routinely used to decide breast cancer prognostication and postsurgical adjuvant therapy, we constructed a tissue microarray from 97 breast tumors, with a single 0.6 mm core per specimen. Immunostaining of tissue microarray sections and conventional full sections of each tumor were performed using well-characterized prognostic markers (estrogen receptor ER, progesterone receptor PR and c-erbB2). The full section versus tissue microarray concordance for these stains was 97% for ER, 98% for PR, and 97% for c-erbB2, respectively, with a strong statistical association (kappa value more than 0.90). Fluorescence in situ hybridization analysis for HER-2/neu gene amplification from the single-core tissue microarray was technically successful in about 90% (87/97) of the cases, with a concordance of 95% compared with parallel analyses with the full sections. The correlation with other pathological parameters was not significantly different between full-section and array-based results. It is concluded that the constructed tissue microarray with a single core per specimen ensures full biological representativeness to identify the associations between biomarkers and clinicopathological parameters, with no significant associated sampling bias. 相似文献
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