RP-HPLC法测定钩吻生物碱

用RP-HPLC法分离和测定了钩吻碱甲(G),钩吻碱戊(F),胡蔓藤碱丙(D)、胡蔓藤碱乙(B)及胡蔓藤碱甲(A)。用常山碱为内标。分析柱C₁₈,甲醇—水—正丁胺(78:22:0.1 V/V)为流动相,流速1.0 ml/min,检测波长256 nm。线性范围0.02～0.12μg,相关系数r=0.9835～0.9977,回收率95.01～99.70%。本方法简便、灵敏、重现性好,为生产及临床用药提供质量控制依据。     

Trauma of the extremities: prospective comparison of digital and conventional angiography

Digital subtraction angiography (DSA) and conventional film angiography (CFA) were compared prospectively in 50 patients with trauma of the extremities. Each patient underwent both procedures with comparable imaging parameters and injections. Three angiographers reviewed the angiograms for the presence of seven angiographic signs of arterial injury. The two modalities showed pseudoaneurysms, arteriovenous fistulas, vessel displacement, occlusion, and focal narrowing equally well. CFA was superior to DSA in delineating intimal dissection; DSA was better for demonstrating extravasation. Clinical follow-up demonstrated that both procedures had a sensitivity of 100%; the specificity was 94% for DSA and 97% for CFA. Neither procedure produced a false-negative result.     

苦木中生物碱测定方法的研究

本文报道了在高效硅胶薄层上用氯仿(水饱和)—甲醇(40:0.5)展开系统,分离了苦木中三种咔巴林生物碱(Ⅰ,Ⅲ,Ⅳ)及一种铁屎米酮生物碱(Ⅱ)。用甲酸处理后,薄层扫描法测定。本法决速、灵敏,检出限度可达1×10^-8g,各生物碱的线性范围为0.05～0.3μg,并用此法测定了十一个不同地区及不同部位的苦木生药中的生物碱含量。     

An unusual consequence of stereotactic colloid cyst aspiration: Case report

The removal of a colloid cyst of the third ventricle by stereotactic cyst aspiration is frequently performed as an alternative to a transcortical-transventricular or transcallosal operative route. A consequence of a CT-guided stereotactic aspiration, where residual colloid cyst material has been dislodged into the lateral ventricle and likened to an intraventricular mouse, is described.     

A randomized controlled trial of an intervention designed to improve the care given in general practice to Type II diabetic patients: patient outcomes and professional ability to change behaviour

AIM: Our objective was to evaluate the effect of training in a patient- centred intervention for GPs and practice nurses on outcomes for patients with Type II diabetes. METHODS: We carried out a randomized controlled trial within general practices as the basis for randomization and a before-and-after design for measures of patient outcome. A parallel process study examined the use of the method by professionals. The study was carried out in 29 general practices in South Glamorgan who had participated for at least 2 years in a local scheme of audit and CME in relation to Type II diabetes care. The subjects were 252 Type II diabetic patients recruited by 15 experimental and 14 control practices. The main outcome measures were changes in glycosylated haemoglobin, patient satisfaction with care and treatment, functional health status and professional ability to apply the intervention. RESULTS: Professionals adopted the innovative method with enthusiasm, but after 2 years only 19% continued to apply the method systematically. The trial was, therefore, unable to demonstrate significant biochemical or functional improvements. This highlights the need to understand the factors associated with professional uptake and subsequent ability to sustain changes in behaviour. CONCLUSIONS: The efficacy of this behavioural intervention remains unproved, despite its acceptability to professional staff. Detailed and prolonged development and testing of behavioural interventions is an essential first step before embarking on randomized controlled trials which involve complex behavioural changes in professionals or patients.      

RP－HPLC法测定牛蒡子中木脂素的含量

用ＲＰ－ＨＰＬＣ法分离并测定了牛蒡子中５种２，３－二苄基丁内酯型木脂素——牛蒡甙（Ⅰ）、牛蒡酚Ａ（Ⅱ）、牛蒡酚Ｆ（Ⅲ）、牛蒡甙元（Ⅳ）、牛蒡素Ｂ（Ⅴ）。以安定为内标，分析柱Ｃ１８，甲醇－水－乙腈－四氢呋喃（５７：４９：１１：１）为流动相，梯度流速，１．０～１．５ｍ１／ｍｉｎ。检测波长２２０ｎｍ，线性范围０．０２９～０．２４２μｇ，相关系数ｒ＝０．９９９３～０．９９９９，回收率９６．１４～１０４．９９％。本法简便、快速、灵敏。     

RP-HPLC法测定牛蒡子中木脂素的含量

用RP-HPLC法分离并测定了牛蒡子中5种2,3-二苄基丁内酯型木脂素——牛蒡甙(Ⅰ)、牛蒡酚A(Ⅱ)、牛蒡酚F(Ⅲ)、牛蒡甙元(Ⅳ)、牛蒡素B(Ⅴ)。以安定为内标,分析柱C₁₈,甲醇-水-乙腈-四氢呋喃(57:49:11:1)为流动相,梯度流速,1.0～1.5m1/min。检测波长220nm,线性范围0.029～0.242μg,相关系数r=0.9993～0.9999,回收率96.14～104.99%。本法简便、快速、灵敏。     

Manpower, entrepreneurs, and antitrust

The problem of nonradiologists practicing radiology is discussed. The trend toward moving the practice of radiology out of the hospital and into the office setting favors the entrepreneur, radiologist and nonradiologist alike. Many clinicians are setting up offices with radiological facilities. Because of the threat of antitrust litigation, radiological societies may be discouraged from speaking out against this practice.