全文获取类型
收费全文 | 1726篇 |
免费 | 142篇 |
国内免费 | 56篇 |
专业分类
耳鼻咽喉 | 28篇 |
儿科学 | 22篇 |
妇产科学 | 17篇 |
基础医学 | 138篇 |
口腔科学 | 59篇 |
临床医学 | 176篇 |
内科学 | 249篇 |
皮肤病学 | 26篇 |
神经病学 | 39篇 |
特种医学 | 82篇 |
外科学 | 285篇 |
综合类 | 334篇 |
一般理论 | 1篇 |
预防医学 | 134篇 |
眼科学 | 7篇 |
药学 | 163篇 |
2篇 | |
中国医学 | 95篇 |
肿瘤学 | 67篇 |
出版年
2024年 | 8篇 |
2023年 | 10篇 |
2022年 | 37篇 |
2021年 | 54篇 |
2020年 | 40篇 |
2019年 | 27篇 |
2018年 | 29篇 |
2017年 | 41篇 |
2016年 | 35篇 |
2015年 | 88篇 |
2014年 | 82篇 |
2013年 | 113篇 |
2012年 | 141篇 |
2011年 | 167篇 |
2010年 | 121篇 |
2009年 | 116篇 |
2008年 | 103篇 |
2007年 | 100篇 |
2006年 | 91篇 |
2005年 | 94篇 |
2004年 | 61篇 |
2003年 | 71篇 |
2002年 | 47篇 |
2001年 | 22篇 |
2000年 | 24篇 |
1999年 | 21篇 |
1998年 | 21篇 |
1997年 | 18篇 |
1996年 | 17篇 |
1995年 | 12篇 |
1994年 | 8篇 |
1993年 | 18篇 |
1992年 | 4篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 10篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1973年 | 2篇 |
1962年 | 2篇 |
排序方式: 共有1924条查询结果,搜索用时 234 毫秒
21.
彩色多普勒超声心动图诊断肺心病的临床价值 总被引:1,自引:1,他引:0
目的:阐述彩色多普勒超声心动图(CDE)诊断肺心病的临床价值。方法:通过CDE对临床上拟诊的肺心病患者进行肺动脉高压(PH)定性、定量的诊断、并观测由PH继发引起心脏在CDE上的异常改变。结果:CDE测定PH44例(88%),其肺动脉收缩压(SPAP)>36mmHg,6例(32mmHg≤SPAP<36mmHg,确诊CPM的临床符合率665(50/76)。结论作为无创性诊技术,CDE估测PH或诊断CPM准确简便,重复性强,对于指导临床治疗意义重大。 相似文献
22.
目的 为了了解不良妊娠中TORCH感染的影响。方法 应用酶联免疫吸附试验 (ELISA)对 31例不良妊娠及 43例正常妊娠的孕妇进行TORCH感染监测。结果 两组感染率最高均为CMV ,其次为HSVⅠ 。两组相比CMV有极显著性差异 (P <0 .0 0 5 ) ;HSVⅠ 有显著差异 (P <0 .0 5 )。两组IgG检出率最高均为CMV ,分别为 96 .77%及 6 9.77% ,最低均为TOXO ,分别为 2 .9%、9.3% ;两组的IgM检出率最高均为CMV ,分别为 35 .48%、16 .2 8%。两组易感染率最高均为TOXO。结论 CMV、HSVⅠ 是本地区TORCH感染中对妊娠影响最大的病原体 ,二者均为性传播疾病 ,对其危害不容忽视 相似文献
23.
目的:检测B7基因在肾细胞癌的表达及其与肾细胞癌分级、分期及预后的关系。方法:本文应用RTPCR和免疫组织化学技术检测了22例肾细胞癌组织B7的表达。结果:RTPCR5例阳性,免疫组化4例阳性,B7mRNA表达与肾癌病理分级、分期无关系;病理切片HE染色显示,B7阳性肿瘤组织内淋巴细胞浸润明显增多。结论:表达B7的肿瘤组织局部抗肿瘤免疫反应增强,提示B7表达可能与肿瘤预后有关。大部分泌尿系肿瘤不表达B7,为转B7基因治疗泌尿系肿瘤提供了理论依据。 相似文献
24.
蒙药忠论-5胶囊总生物碱测定及其体外溶出度研究 总被引:2,自引:0,他引:2
目的 考察蒙药忠伦-5胶囊中总生物碱含量及其体外溶出情况。方法 以苦参碱为对照品,利用酸性染料比色法测定总生物碱;以水为介质,用转篮法考察其体外溶出度。结果 含量测定方法精密度为RSD=1.89%,平均回收率为100.89%,RSD=3.49%。;苦参总生物碱相对累积溶出度与溶出时间相关显著(P<0.01),T50=24min,Td=31min,m=1.32。结论 用上法测定总生物碱含量及体外溶出度,对评估忠伦-5胶囊质量有实用价值。 相似文献
25.
This study was performed to investigate whether the toxic effects of Loxosceles gaucho venom on cells might be exerted via stimulators of TNF-alpha release generated by sphingomyelinase D--a major component of the venom. It was demonstrated that L. gaucho venom alone is unable to induce TNF-alpha release by J774A.1 cells, while in the presence of exogenous sphingomyelin it induces a high level of TNF-alpha release which is significantly increased by incubation with non-inactivated serum. Ceramide phosphate also induces TNF-alpha release in J774A.1 cells, but (unlike sphingomyelin/sphingomyelinase) the level of release is not influenced by the presence or otherwise of non-inactivated serum. L. gaucho venom does not induce proliferation of J774A.1 cells and even at high concentrations it does not affect their viability. J774A.1 cells, which prior to venom treatment were elongated and clumped, round up after venom treatment, but, revert to their original morphology after incubation with fresh medium. TNF-alpha resistant MRC-5 cells and TNF-alpha sensitive MCF-7 cells are susceptible to the toxic effect of both L. gaucho venom and ceramide phosphate. The results obtained in this study demonstrate that exogenous sphingomyelin can modulate, in vitro, the release of TNF-alpha induced by L. gaucho venom in mouse macrophages. In addition, the results also indicate that ceramide phosphate and L. gaucho venom are toxic to several different cell types, via a variety of mechanisms, some, but not all, of which may involve TNF-alpha as an intermediary. 相似文献
26.
27.
Pediatric renal transplantation in Santa Cruz hospital 总被引:2,自引:0,他引:2
Branco P Jorge C Gaspar A Domingos F Machado D Neto A Serra Sousa J Pina A Messias H 《Transplantation proceedings》2000,32(8):2615-2616
28.
S Bydder NA Spry DRH Christie D Roos BH Burmeister H Krawitz S Davis DJ Joseph M Poulsen M Berry 《Journal of Medical Imaging and Radiation Oncology》2003,47(3):284-288
The purpose of this study was to prospectively examine the effectiveness and tolerability of a simple radiotherapy technique for the palliation of symptomatic liver metastases. Twenty‐eight patients with symptomatic liver metastases were enrolled from seven centres, and received targeted (partial or whole) liver irradiation consisting of 10 Gy in two fractions over 2 days. Symptoms at baseline were hepatic pain (27 patients), abdominal distension (19), night sweats (12), nausea (18) and vomiting (eight). Twenty‐two patients (76%) had failed previous treatment with chemotherapy, hormonal therapy and/or high‐dose steroids. Symptoms and potential toxicities were prospectively assessed at the time of treatment, then 2, 6 and 10 weeks later. Individual symptom response rates were 53?66% at 2 weeks. Partial or complete global symptomatic responses were noted in 15 patients (54%) overall. The treatment was well tolerated with two patients (7%) experiencing grade 3 toxicity (one vomiting and one diarrhoea); however, four patients reported temporary worsening of pain shortly after treatment. This simple and well‐tolerated treatment achieves useful palliation. 相似文献
29.
Aberrant crypt focus promotion and glucose intolerance: correlation in the rat across diets differing in fat, n-3 fatty acids and energy 总被引:1,自引:0,他引:1
Koohestani N; Chia MC; Pham NA; Tran TT; Minkin S; Wolever TM; Bruce WR 《Carcinogenesis》1998,19(9):1679-1684
McKeown-Eyssen (Cancer Epidemiol. Biomarkers Prevent., 3, 687-695, 1994)
and Giovannucci (Cancer Causes Control, 6, 164-179, 1995), noting the
striking similarity in lifestyle risk factors for colorectal cancer and
insulin resistance, proposed that the hyperinsulinemia, glycemia and
hypertriglyceridemia associated with insulin resistance promotes colon
cancer. To compare the effect of diet on colon cancer promotion and insulin
resistance in the F344 rat, we assessed the effect of fat, n-3 fatty acids
and energy in pairwise comparisons on average size of aberrant crypt foci
(ACF) and on glucose intolerance in the same animals in a single
experiment. Diets high in fat and energy increased and diets with increased
n-3 fatty acids and calorie restriction decreased both ACF growth and
glucose intolerance compared with control diets. The measures of promotion
of colon cancer and insulin resistance were strongly correlated (n = 98, r
= 0.67, P < 0.001). In addition, both were highly correlated with daily
energy intake (r = 0.62 and 0.66) and were also correlated with basal
(post-prandial) insulin, glucose and triglycerides (r = 0.31-0.53, P <
0.01). We concluded that ACF growth and glucose intolerance are correlated
for a wide range of diets and that increased circulating energy (glucose
and triglycerides) may lead to both colon cancer promotion and insulin
resistance.
相似文献
30.
目的在β-受体激动情况下,研究多非替利衍生物CPU 228对电刺激触发心肌细胞的胞浆Ca2+浓度([Ca2+]i)变化及钙瞬变动力学参数的影响.方法游离单个大鼠心室肌细胞,Fluo-3/AM负载,电刺激诱发心室肌细胞钙瞬变,定量测定心室肌细胞内Ca2+浓度变化,异丙肾上腺素(isoproterenol, ISO)100nmol·L-1激动β-受体,观察CPU 228 1 μmol·L-1对钙瞬变动力学参数、[Ca2+]i及钙负荷水平的影响.结果CPU 228 1 μmol·L-1对大鼠心室肌细胞[Ca2+]i的影响不明显(P>0.05);ISO 100 nmol·L-1显著升高大鼠心室肌细胞[Ca2+]i和细胞内钙负荷水平,缩短钙瞬变时程,并且增加钙瞬变的消除速率.CPU 228 1 μmol·L-1显著抑制ISO的上述作用.结论在β-受体激动情况下,CPU 228可以降低心肌细胞[Ca2+]i,使ISO改变的钙瞬变动力学参数恢复到正常水平. 相似文献