全文获取类型
收费全文 | 90篇 |
免费 | 3篇 |
专业分类
儿科学 | 1篇 |
妇产科学 | 1篇 |
基础医学 | 17篇 |
口腔科学 | 7篇 |
临床医学 | 7篇 |
内科学 | 26篇 |
皮肤病学 | 1篇 |
神经病学 | 5篇 |
特种医学 | 1篇 |
外科学 | 4篇 |
预防医学 | 9篇 |
药学 | 11篇 |
中国医学 | 1篇 |
肿瘤学 | 2篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 11篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2014年 | 5篇 |
2013年 | 3篇 |
2012年 | 5篇 |
2011年 | 11篇 |
2010年 | 4篇 |
2009年 | 2篇 |
2008年 | 5篇 |
2007年 | 5篇 |
2006年 | 2篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 3篇 |
2002年 | 1篇 |
2001年 | 4篇 |
1998年 | 1篇 |
1991年 | 1篇 |
1988年 | 3篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有93条查询结果,搜索用时 15 毫秒
81.
82.
Anna Popova Julia Kzhyshkowska Dinara Nurgazieva Sergij Goerdt Alexei Gratchev 《Immunobiology》2011,216(1-2):164-172
Macrophages play a key role in inflammation, tissue regeneration and tolerance. Their differentiation is regulated by tissue cells derived CSF-1 (M-CSF). The ability of macrophages to use autocrine M-CSF to control their differentiation and function remained controversial. In this study we investigated the regulation of M-CSF production by Th1 and Th2 cytokines (IFN-γ and IL-4) and tolerogenic stimuli – glucocorticoid dexamethasone in primary human monocyte derived macrophages. We show that IFN-γ and IL-4 efficiently induce production of M-CSF while glucocorticoid inhibited it in a dose dependent manner. Since glucocorticoid inhibits production of inflammatory cytokines we tested whether this effect is a result of inhibited M-CSF production. We showed that exogenous M-CSF rescues the ability of glucocorticoid-treated macrophages to produce TNF and IL-6 in response to LPS. These data indicate that glucocorticoid-treated macrophages retain the ability to respond to M-CSF. Analyzing the mechanism of this responsiveness, we showed that dexamethasone up-regulates surface expression of M-CSF receptor – CSF-1R. We conclude that the ability of macrophages to produce M-CSF secures macrophage differentiation under Th1 and Th2 conditions if tissue cells are unable to supply enough M-CSF. Increased surface expression of CSF-1R in tolerogenic conditions guarantees response to minute amounts of exogenous M-CSF. 相似文献
83.
84.
Hasija R Pistorio A Ravelli A Demirkaya E Khubchandani R Guseinova D Malattia C Canhao H Harel L Foell D Wouters C De Cunto C Huemer C Kimura Y Mangge H Minetti C Nordal EB Philippet P Garozzo R Martini A Ruperto N;Pediatric Rheumatology International Trials Organization 《Arthritis and rheumatism》2011,63(10):3142-3152
85.
86.
Joaquim S. Costa Júnior Alexandre B.F. Ferraz Taciana O. Sousa Romézio A.C. Silva Sidney G. De Lima Chistiane M. Feitosa Antônia M.G.L. Citó Ana A.C. Melo Cavalcante Rivelilson M. Freitas Angelo R. Moura Sperotto Valéria F. Péres Dinara J. Moura Jenifer Saffi 《Basic & clinical pharmacology & toxicology》2013,112(1):34-41
Platonia insignis Mart., a native species of the Brazilian Amazon more commonly known as bacuri, is a member of the Clusiaceae family. In this study, we evaluated the chemical composition and the antioxidant and toxicity activities of the dichloromethane and ethyl acetate fractions from P. insignis seed ethanolic extract using different experimental models. Our results demonstrate in vitro antioxidant effects, by 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid) diammonium salt and 1,1‐diphenyl‐2‐picryl‐hydrazyl assays, as well as in vivo effects in antioxidant‐defective Saccharomyces cerevisiae strains to both fractions. Toxicity was evaluated against the micro‐crustaceous Artemia salina Leach. and promastigote Leishmania amazonensis. The dichloromethane fraction was the most active fraction evaluated on A. salina and promastigote L. amazonensis (IC50 = 24.89 μg/mL and 2.84 μg/mL, respectively). In addition, a slight cytotoxicity was observed in mammalian V79 cells using ethyl acetate and dichloromethane fractions with MTT assays. Both fractions displayed genotoxicity up to 25 μg/mL (dichloromethane) and 10 μg/mL (ethyl acetate) in V79 cells, as evaluated by the alkaline comet assay. Thus, in this study, we demonstrate for the first time that ethyl acetate and dichloromethane fractions from P. insignis seeds display antioxidant effects, a toxic effect against A. salina and L. amazonensis and induce genotoxicity in V79 mammalian cells. The observed activities can be attributed to the phenolic compounds present in these fractions and to the presence of xanthones (alpha‐ and gamma‐mangostin). 相似文献
87.
Dmitriy Devetiarov Ulia Semenova Svetlana Usova Alexey Tomskiy Vladimir Tyurnikov Dinara Nizametdinova Artem Gushcha Elena Belova Alexey Sedov 《Clinical neurophysiology》2017,128(12):2482-2490
Objective
The aim of this study was to distinguish neuronal activity patterns in the human ventral thalamus and reveal common and disease-specific features in patients with Parkinson’s disease (PD) and cervical dystonia (CD).Methods
Single unit activity of neurons was recorded during microelectrode-guided thalamotomies. We classified neurons of surgical target and surrounding area into patterns and compared their characteristics and responsiveness to voluntary movement between PD and CD patients.Results
We distinguished five patterns of neuronal activity: single, LTS burst, mixed, non-LTS burst and longburst patterns. The burst and mixed patterns showed significant differences in several basic and burst characteristics. We showed that there were no disease-specific patterns or significant differences in pattern distribution between studied patients. However, burst patterns had an unbalanced distribution between disease conditions. In addition, we found difference in LTS burst characteristics between surgical targets and surrounding nuclei. All identified patterns, except the long burst pattern, were reactive to the motor tasks and to contraction of the pathological muscles.Conclusions
The ventral thalamus was characterised by common neuronal activity patterns which differed in characteristics between PD and CD.Significance
Our findings highlight patterns of neuronal activity of the human ventral thalamus and specific pathological features. 相似文献88.
X-ray image of changes in facial skull bones and temporomandibular articulation in 75 children and adolescents with a history of traumatic or inflammatory diseases or surgery for benign mandibular tumors in childhood is described. The deformation type did not present the specific features indicating the cause of the defect; its severity depended on the length of the defect existence and the depth of the injury to the mandibular growth area. Deformations of the lower third of the facial skull commonly develop soon after the underlying disease; changes in the upper segments of the skull develop later. If skin plasty was carried out simultaneously with tumor removal no facial skull deformations developed or these deformations were but negligible. The longer was the existence of the defect, the more coarse and vast were the changes in facial skull bones, that needed a combined surgical and orthodontic management. 相似文献
89.
Gilyazova DG Rosenkranz AA Gulak PV Lunin VG Sergienko OV Khramtsov YV Timofeyev KN Grin MA Mironov AF Rubin AB Georgiev GP Sobolev AS 《Cancer research》2006,66(21):10534-10540
A major problem in the treatment of cancer is the specific targeting of drugs to these abnormal cells. Ideally, such a drug should act over short distances to minimize damage to healthy cells and target subcellular compartments that have the highest sensitivity to the drug. We describe the novel approach of using modular recombinant transporters to target photosensitizers to the nucleus, where their action is most pronounced, of cancer cells overexpressing ErbB1 receptors. We have produced a new generation of the transporters consisting of (a) epidermal growth factor as the internalizable ligand module to ErbB1 receptors, (b) the optimized nuclear localization sequence of SV40 large T-antigen, (c) a translocation domain of diphtheria toxin as an endosomolytic module, and (d) the Escherichia coli hemoglobin-like protein HMP as a carrier module. The modules retained their functions within the transporter chimera: they showed high-affinity interactions with ErbB1 receptors and alpha/beta-importin dimers and formed holes in lipid bilayers at endosomal pH. A photosensitizer conjugated with the transporter produced singlet oxygen and (*)OH radicals similar to the free photosensitizer. Photosensitizers-transporter conjugates have >3,000 times greater efficacy than free photosensitizers for target cells and were not photocytotoxic at these concentrations for cells expressing a few ErbB1 receptors per cell, in contrast to free photosensitizers. The different modules of the transporters, which are highly expressed and easily purified to retain full activity of each of the modules, are interchangeable, meaning that they can be tailored for particular applications. 相似文献
90.
Sarybaev AS Palasiewicz G Usupbaeva DA Plywaczewski R Maripov AM Sydykov AS Mirrakhimov MM Le Roux H Kadyrov T Zielinski J 《High altitude medicine & biology》2003,4(4):455-463
Chronic alveolar hypoxia due to disease or low atmospheric pressure at high altitude results in the development of hypoxic pulmonary hypertension. The effects of intermittent hypoxia on pulmonary hemodynamics in healthy men have not been studied. We aimed to investigate, prospectively, pulmonary hemodynamics in workers commuting between an elevation of 3700 and 4200 m (4-week working shift) and lowland, below 500 m (4 weeks of holiday). Pulmonary hemodynamics has been investigated by Doppler echocardiography in 26 healthy Caucasian males, mean age 42 +/- 9 yr. First at lowland (760 m) and next during the fourth week of work at high altitude. Investigations were repeated in 21 subjects 1 year later at the end of the high-altitude exposure. The third series of investigations was performed 2 yr after the initial ones in 10 subjects who earlier had shown the strongest hypoxic vasoconstriction. At lowland, subjects presented with normal pulmonary hemodynamics. At high altitude, mean pulmonary artery pressure (PAPm) rose from 14.7 +/- 2.7 mmHg to 25.8 +/- 8.3 mmHg. One year later the PAPm remained unchanged in hypoxic conditions (25.0 +/- 7.3 mmHg). At the end of a 2-year follow-up of 10 "hyperreactors," PAPm measured at the end of the hypoxic exposure was the same as at the initial investigation, averaging 28 +/- 4.0, 28 +/- 3.5, and 29 +/- 2.5 mmHg at the beginning and at 1 and after 2 yr of intermittent exposure to high altitude. We concluded that intermittent exposure to 4000 m lasting 3 yr does not lead to development of permanent pulmonary hypertension. 相似文献