音乐疗法对慢性精神分裂症病人疗效观察

精神分裂症是以基本个性改变,思维、情感、行为的分裂,精神活动的不协调为主要特征的一类最常见的精神症,病因尚示阐明,多在青壮年起病,病程迁延,缓慢进展,有发展为衰退的可能。其临床症状多表现为思维联想障碍、情感障碍、意志行为障碍、幻觉和感知综合障碍、妄想、紧张综合征     

缺血预处理对缺血骨骼肌收缩功能的影响

背景缺血预处理能有效提高骨骼肌缺血耐受性,减轻骨骼肌缺血再灌注期间的坏死范围,但缺血预处理对骨骼肌收缩功能的影响文献报道不多.目的探讨缺血预处理对骨骼肌缺血再灌注期间收缩功能的影响.设计以实验动物为研究对象的随机对照研究.单位华中科技大学同济医学院及解放军第二五二医院.材料实验地点为解放军第二五二医院中心实验室.选用健康雄性SD大鼠14只.方法采用大鼠后肢缺血再灌注模型,将14只大鼠随机分为对照组和实验组.对照组持续缺血4 h,再灌注1 h;实验组缺血5 min,再灌注5 min,重复3次后,持续缺血4 h再灌注1 h.测定缺血再灌注期间腓肠肌收缩功能变化及再灌注1 h后,血清磷酸激酶(CK),丙二醛和腓肠肌99锝m亚甲基二磷酸钠(99TcmMDP)吸收量变化.主要观察指标缺血预处理对腓肠肌收缩力及对血清CK,丙二醛及99TcmMDP吸收量的影响.结果实验组腓肠肌收缩力在缺血4h时为(14.32±5.05)g,再灌注1 h时为(25.71±7.58)g,对照组分别为(0,4 73±2.05)g,两者相比差异有显著性意义(P＜0.05),实验组血清CK为(104.85±9.84)nkat/L,丙二醛为(3988.60±455.92)nmol/L,99TcmMDP吸收量为(56.0±8.1)mBq/g·mir,对照组CK为(136.36±14.50)nkat/L,丙二醛为(6 542.90±536.72)nmol/L,99TcmMDP吸收量为(97.3±5.8)mBq/g·min,两者相比差异有显著性意义(P＜0.05,P＜0.01).结论缺血预处理能有效改善缺血再灌注期间骨骼肌的收缩力,减轻骨骼肌坏死程度.因此,缺血预处理对缺血骨骼肌的收缩功能具有保护作用.     

彩色多普勒血流显像检测妊娠高血压综合征患者及其胎儿肾动脉阻力指数的意义

本文应用CDFI检测63例妊高征治疗前、不同治疗后母体及其胎儿肾动脉血流阻力指数（RI）并计算个体间的差值百分比。结果显示：①单纯吸氧治疗后胎儿肾动脉RI下降幅度较母体肾动脉RI下降幅度大（P＜0.01），表明妊高征时胎儿肾动脉阻力增高与缺氧关系密切;②单纯解痉治疗后，母体肾动脉RI较胎儿肾动脉RI下降幅度大（P＜0.01）。表明妊高征时母体肾动脉阻力增高的主要原因是血管痉挛。     

血游离甲状腺激素测定对甲亢的诊断价值

作者用放免法测定了57例甲亢患者血中游离甲状腺激素(FT_3、FT_4)水平,并与总甲状腺激素(TT_3、TT_4)测定结果进行了比较。结果表明:FT_3诊断符合率达100％,FT_3及FT_4升高的幅度比TT_3及TT_4大,对TT_3及TT_4升高不明显或在正常高限的病人,测FT_3及FT_4有诊断价值;对不典型甲亢的诊断及甲亢治疗期代谢控制情况的判断,FT_3及FT_4优于TT_3及TT_4。     

Multiple occipital defects caused by arachnoid granulations: Emphasis on T2 mapping

A 56-year-old man presented with a 6-mo history of headache. Although neurological and laboratory examinations were normal, computed tomography (CT) scan was performed which revealed multiple occipital osteolytic lesions, which were suspected to be multiple myeloma. Subsequently nuclear magnetic resonance imaging (MRI) showed that these lesions presented with a cerebrospinal fluid (CSF)-like signal intensity, no diffusional restriction and intrinsic mass-like enhancement on conventional sequences were seen. T2 relaxation time was similar to that of CSF in the ventricles and adjacent subarachnoid space on T2-mapping. Single photon emission CT with 99m Tc-Methyl diphosphonate was performed which revealed no increased radiotracing accumulation. Finally, these lesions were diagnosed as mutiple arachnoid granulations (AGs). The headache was treated symptomatically with medical therapy. On follow up examination after 6 mo no evidence of tumor was detected. This report aimed to illustrate the appearance and differentiation of occipital defects caused by multiple AGs on CT and MRI, with emphasis on the findings from T2 mapping.     

北方汉族人原发性高血压易感性与肿瘤坏死因子α和转化生长因子β基因型多态性的关联

目的：探讨中国北方地区汉族人肿瘤坏死因子α和转化生长因子β基因型的多态性与原发性高血压发病的相关性。 方法：①选择2004-06／2005—01哈尔滨市第一医院门诊和住院原发性高血压患者36例为高血压组，男20例，女16例。选择2004—04／2005-12哈尔滨市第一医院健康体检者40人为对照组，男23人，女17人。均为北方汉族，且均对检测项目知情同意。②采用顺序特异性引物聚合酶链反应方法检测肿瘤坏死因子α和转化生长因子β基因型别多态性变化。③计数资料差异性比较采用χ^2检验。 结果：原发性高血压患者36例和健康者40人均进入结果分析。①原发性高血压组肿瘤坏死因子α（308A）等位基因频率明显高于对照组（42％，10％，P〈0．01）。②两组转化生长因子β基因频率差异不明显（P〉0．05）。 结论：中国北方地区汉族人肿瘤坏死因子α（308h）等位基因位点的多态性可能与原发性高血压的易感性相关联。     

Short-term Rosuvastatin Treatment for the Prevention of Contrast-induced Acute Kidney Injury in Patients Receiving Moderate or High Volumes of Contrast Media: A Sub-analysis of the TRACK-D Study

Background:

Current randomized trials have demonstrated the effects of short-term rosuvastatin therapy in preventing contrast-induced acute kidney injury (CIAKI). However, the consistency of these effects on patients administered different volumes of contrast media is unknown.

Methods:

In the TRACK-D trial, 2998 patients with type 2 diabetes and concomitant chronic kidney disease (CKD) who underwent coronary/peripheral arterial angiography with or without percutaneous intervention were randomized to short-term (2 days before and 3 days after procedure) rosuvastatin therapy or standard-of-care. This prespecified analysis compared the effects of rosuvastatin versus standard therapy in patients exposed to (moderate contrast volume [MCV], 200–300 ml, n = 712) or (high contrast volume [HCV], ≥300 ml, n = 220). The primary outcome was the incidence of CIAKI. The secondary outcome was a composite of death, dialysis/hemofiltration or worsened heart failure at 30 days.

Results:

Rosuvastatin treatment was associated with a significant reduction in CIAKI compared with the controls (2.1% vs. 4.4%, P = 0.050) in the overall cohort and in patients with MCV (1.7% vs. 4.5%, P = 0.029), whereas no benefit was observed in patients with HCV (3.4% vs. 3.9%, P = 0.834). The incidence of secondary outcomes was significantly lower in the rosuvastatin group compared with control group (2.7% vs. 5.3%, P = 0.049) in the overall cohort, but it was similar between the patients with MCV (2.0% vs. 4.2%, P = 0.081) or HCV (5.1% vs. 8.8%, P = 0.273).

Conclusions:

Periprocedural short-term rosuvastatin treatment is effective in reducing CIAKI and adverse clinical events for patients with diabetes and CKD after their exposure to a moderate volume of contrast medium.     

Proteomic analysis of exosomes derived from human lymphoma cells

Background

Exosomes secreted by tumor cells contain specific antigens that may have immunotherapeutic purposes. The aim of this study was to characterize the proteomic content of lymphoma cell-derived exosomes (LCEXs).

Methods

In this study, exosomes derived from Raji cells (EXO_Raji) were purified and proteins of EXO_Raji were separated by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein bands were identified by mass spectrometry. The protein components of EXO_Raji were analyzed using shotgun technology, and the function proteins of EXO_Raji were defined and described using the Gene Ontology (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.

Results

A total of 197 proteins were identified in EXO_Raji; 139 proteins were also identified in Raji cells, showing an overlap of 70.56% of the total proteins in EXO_Raji. Interestingly, the remaining 58 proteins were unique to EXO_Raji. The GO database and KEGG were used to define and describe the function of proteins. The data showed that some important proteins involved in antigen procession and presentation as well as cell migration and adhesion were also identified in EXO_Raji, such as MHC-I and II, HSC70, HSP90, and ICMA-1.

Conclusions

LCEXs express a discrete set of proteins involved in antigen presentation and cell migration and adhesion, suggesting that LCEXs play an important role in the regulation of immunity and interaction between lymphoma cells and their microenvironment. LCEXs harbor most of the proteins of lymphoma cells and could be one of the sources of lymphoma-associated antigens for immunotherapeutic purposes.     

High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats

Background: The failure of intestinal mucosal barrier may induce multiple organ dysfunction and systemic inflammatory response syndrome, but little work has been done on whether hypobaric hypoxia related to the failure of intestinal mucosal barrier. Aims: To study the expression of hypoxia-inducible factor 1α (HIF-1α), inducible nitric oxide synthase (iNOS) and morphological changes of intestinal mucosa in albino rats at different altitude. Methods: 30 male Wistar rats raised in plain for one month were randomly divided into 3 groups: Plain 500 m group (n=10), High-altitude (HA) 3842 m group (n=10) and HA4767 m group (n=10). Each group was delivered to different altitude area at the same shipping time and executed after 3 days’ exposure to different altitude. Intestinal segments with the same location of all rats were removed for morphological analyses. Morphologic parameters (villous height, crypt depth, mucosal wall thickness and villous surface area) were measured by optical and scanning electron microscope. The expression of iNOS and HIF-1α were detected by immunohistochemistry. Results: Morphological indexes in higher altitude groups were exacerbated obviously compared with those of lower altitude groups. While the expression of iNOS and HIF-1α in higher altitude groups were significantly increased than those of lower altitude groups. Linear correlation analysis showed that the expression of iNOS was positively correlated with that of HIF-1α. Conclusions: Hypobaric hypoxia increases the expression of HIF-1α and iNOS in intestinal mucosa, however exacerbates the mucous morphologic parameters with altitude increasing. HIF-1α may regulate the expression of iNOS and be involved in the damage of intestinal mucosa.     

Inhibition of SALL4 suppresses carcinogenesis of colorectal cancer via regulating Gli1 expression

Background: SALL4 is a novel oncogene mediating tumorigenesis in multiple carcinomas. However, its actual role and mechanisms participating in the development of colorectal cancer remains unclear. Methods: Immunohistochemical staining and Western blot were conducted to detect the expression of SALL4 and other molecules. siRNA of SALL4 was transfected to silence SALL4 expression in Caco-2 cell line. Flow cytometry was used for cell cycle and apoptosis analysis. Wound healing and transwell assay were used for invasion test. CCK-8 test was employed for cell proliferation and drug sensitivity assessment. Results: By inhibition of SALL4 expression, the proliferation, invasiveness and drug resistance were dramatically reduced while apoptosis rate was up-regulated. Gli1 was found to decrease its expression in SALL4 silencing cells. Moreover, the inhibition on tumorigenesis of Caco-2 by SALL4 silencing was antagonized by Gli1 up-regulation, suggesting Gli1 as a downstream target of SALL4 in cancer development. Conclusion: SALL4 inhibition limited oncogenesis on colorectal cancer by reducing Gli1 expression.