近红外量子点单克隆抗体荧光探针对U14头颈部移植鳞癌的原位可视化成像研究

目的：观察近红外荧光量子点（Near-infrared fluorescent quantum dots,NIRF-QDs）表皮生长因子受体单克隆抗体（Epi－dermal growth factor receptor monoclonal antibody,EGFR mAb）荧光探针对U14头颈部移植鳞癌的原位可视化成像情况。方法：将EGFR mAb偶联发射波长为800 nm的近红外荧光量子点（Quantum dot,QD800）制备近红外量子点表皮生长因子受体单克隆抗体荧光靶向探针（QD800-EGFR mAb）,将高表达表皮生长因子受体的U14鳞癌细胞和QD800-EGFR mAb共培养,观察QD800-EGFR mAb与U14细胞表达的EGFR结合情况;建立U14头颈部移植鳞癌裸鼠模型,通过尾静脉注射QD800-EGFR mAb,观察对U14头颈鳞癌活体原位可视化动态成像情况。结果：体外实验证明：QD800-EGFR mAb能与U14细胞表达的EGFR特异性结合;体内活体成像显示：QD800-EGFR mAb静脉注射后能对U14头颈部移植鳞癌进行清楚的原位可视化荧光成像,荧光成像一直持续到24 h,在1～6 h时间段内肿瘤成像最完整和信噪比最高。结论：基于以EGFR为靶点的NIRF-QDs技术对头颈部鳞癌的发生发展、原位可视化成像和个体化治疗具有独特的优势和发展前景。     

构建区域医疗机构内训师培训体系的实践探索

为了更好地完成国家基本公共服务标准化试点项目,完善区域医疗机构内部人才培训标准化体系,广州医科大学附属第六医院以地市级三级甲等医院优势为依托,以第三方医院管理咨询公司为主要技术支持,与该区域卫生健康局建立长期合作关系,组建区域医疗机构全面质量管理内训师团队,实施标准化课程培训,构建和应用内训师形成性评价体系。逐步完善了区域医疗机构全面质量管理内训师培训标准化体系,培养了一支规模适当、结构合理、素质优良的内训师资队伍。     

预防艾滋病母婴传播抗病毒治疗副作用研究

目的 采用回顾性研究方法分析齐多夫定、奈韦拉平联合用药与单剂量奈韦拉平用药方案在预防艾滋病母婴传播中对肝功能和血象的影响.方法 于2005年5月至2006年10月,对人类免疫缺陷病毒感染的孕产妇109例、婴儿82例应用齐多夫定和奈韦拉平联合用药及单剂量奈韦拉平用药方案的情况进行分析;以第6版医学教材中的血象、肝功正常值为参照进行比较;以国际治疗艾滋病药物毒性指南判定肝功的损害程度.结果 在齐多夫定、奈韦拉平联合用药方案中,母亲服药前后血红蛋白、红细胞平均血红蛋白、总胆红素发生改变,新发贫血15例,但未见粒细胞减少病例;婴儿贫血3例、血小板减少2例、粒细胞减少4例;采用单剂量奈韦拉平用药方案中,母亲产时(服药前)和产后42天的总胆红素、直接胆红素、谷丙转氨酶发生改变;婴儿在出生后6个月出现贫血3例,血小板减少3例,粒细胞减少3例.两种方案进行比较,孕产妇产后42天和婴儿出生后3个月肝功各项指标及婴儿出生后6个月红细胞、血红蛋白、血小板均无统计学意义(P＞0.05).结论 两种方案均对母婴血液系统、肝功系统的影响不大.     

留守因素、家庭关系对留守中学生人格的影响

目的 了解留守因素、家庭关系对留守中学生人格的影响。方法 于2019-2022年采用《艾森克人格问卷》少年版和《家庭亲密度与适应性量表》对眉山市某县级中学1 424名中学生进行调查。结果 纳入的受访者中,616名为留守中学生(占43.26%)。除了人格的精神质维度外,留守与非留守中学生在人格的其余维度及家庭关系的亲密度、适应性两个维度得分比较,差异有统计学意义(P<0.05)。结构方程模型分析显示,在留守初中生中,母亲外出时个体年龄对家庭亲密度有正向影响,路径系数为0.169;家庭亲密度对精神质与神经质有正向影响,路径系数分别为0.310、0.296;父亲在外时间长短对神经质有负向影响,路径系数为-0.146(P<0.05)。在留守高中生中,父亲外出时个体年龄对人格中的内外向有负向影响,路径系数为-0.111;母亲外出时个体年龄对精神质有负向影响,路径系数为-0.125;父亲在外时间长短对家庭亲密度、家庭适应性有负向影响,路径系数分别为-0.146、-0.163;家庭亲密度对精神质、神经质有正向影响,路径系数分别为0.253、0.266;家庭适应性对内外向有负向影响,路径系数为-0.241(P<0.05)。结论 留守经历会影响中学生的人格和家庭关系,但留守初中生和高中生在留守因素、家庭关系对人格特征的影响有所不同。     

江苏省曼氏迭宫绦虫宿主感染调查

目的 了解江苏省曼氏迭宫绦虫宿主感染情况,为曼氏裂头蚴病防控提供科学依据.方法 2018-2019年在江苏省随机抽取9个县(市、区)作为调查点.各调查点随机抽取100名常规健康体检者作为调查对象,进行曼氏迭宫绦虫血清学及病原学检查;在野外环境中调查猫、犬等终宿主及剑水蚤等中间宿主曼氏迭宫绦虫感染情况.结果 江苏省9个调...     

The clinical significance of thyroid hormone-responsive in thyroid carcinoma and its potential regulatory pathway

The study aimed to evaluate the clinical significance of thyroid hormone-responsive (THRSP) and explore its relevant pathways in thyroid carcinoma (THCA).The gene expression data of THRSP were obtained and the prognostic significance of THRSP in THCA was analyzed through various bioinformatics databases. Then, the factors influencing THRSP mRNA expression were explored, and the function of THRSP in predicting the lymph node metastasis (LNM) stage was determined. We further performed the enrichment analysis and constructed a protein–protein interaction (PPI) network to examine potential regulatory pathways associated with THRSP.THRSP gene expression was significantly increased in THCA compared with the normal tissues. High THRSP mRNA expression had a favorable overall survival (OS) in THCA patients (P < .05). Additionally, the mRNA expression of THRSP was related to stage, histological subtype, and methylation among THCA patients (all P < .05). Besides, THRSP served as a potent predictor in discriminating the LNM stage of thyroid cancer patients. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA) on THRSP-associated genes, THRSP was positively related to metabolic pathways.The upregulation of THRSP predicted a good OS in THCA patients. Furthermore, THRSP might inhibit THCA progression through positive regulation of metabolism-associated pathways.     

QRS破碎波与急性心肌梗死患者冠状动脉病变相关参数及病变严重程度相关性研究

目的探讨QRS破碎波(fQRS)与急性心肌梗死(AMI)患者冠状动脉病变相关参数及病变程度的相关性,为临床冠状动脉病变的诊断及治疗提供参考。方法选取自2017年2月至2020年5月亳州市人民医院收治的102例AMI患者为研究对象,根据标准12导联心电图检查是否检出fQRS将患者分为无fQRS组(n=37)与fQRS组(n=65)。记录并比较两组患者心功能指标、实验室检查指标、Gensini评分、病变支数及病变程度,并分析fQRS与冠脉病变相关参数的相关性。结果 fQRS组患者的左室舒张末期内径、肌酸激酶同工酶(CK-MB)、肌钙蛋白I(cTnI)和低密度脂蛋白胆固醇(LDL-C)/高密度脂蛋白胆固醇(HDL-C)均高于无fQRS组,左室射血分数、血流储备分数(FFR)低于无fQRS组,差异有统计学意义(P<0.05)。fQRS组患者的Gensini评分高于无fQRS组;fQRS组患者的双支、多支冠状动脉病变患者比例高于无fQRS组,单支冠状动脉病变患者比例低于无fQRS组;fQRS组患者的中度、重度狭窄患者比例高于无fQRS组,轻度狭窄患者比例低于无fQRS组,差异均有统计学意义...     

团块状灰度变化减轻子宫良性疾病聚焦超声消融术中的远场不良反应

目的：评估团块状灰度变化能否减轻子宫良性疾病聚焦超声消融术中的远场不良反应。方法：开展单中心回顾性临床观察研究,纳入重庆医科大学附属第二医院行聚焦超声消融术的40例子宫良性疾病。比较团块状灰度变化出现前后,在相同能量水平（最小不可耐受能量）下,术中即刻骶尾部或臀部疼痛、放射痛、会阴部痛、肛门胀痛的发生率及不良反应程度。结果：在相同能量水平（最小不可耐受能量）下,团块状灰度变化出现后术中即刻骶尾部或臀部疼痛、放射痛、会阴部痛、肛门胀痛的发生率及不良反应程度明显低于团块状灰度变化出现前（P<0.05）。结论：团块状灰度变化能减轻子宫良性疾病聚焦超声消融术中的远场不良反应。     

A hybrid composite of H2V3O8 and graphene for aqueous lithium-ion batteries with enhanced electrochemical performance

Aqueous rechargeable lithium-ion batteries (ARLBs) are regarded as a competitive challenger for large-scale energy storage systems because of their high safety, modest cost, and green nature. A kind of modified composite material composed of H₂V₃O₈ nanorods and graphene sheets (HVO/G) has been effectively made by a one-step hydrothermal method and following calcination at 523 K. XRD, SEM, TEM, and TG are used to determine the phase structures and morphologies of the composite materials. Owing to the advantage of the layered structure of H₂V₃O₈ nanorods, the excellent conductivity of the graphene sheets, and the 3D network structure of the modified composite, the ARLBs with HVO/G can deliver an adequate specific capacity of 271 mA h g⁻¹ at 200 mA g⁻¹ and have a retention rate of 73.4% after 50 cycles. The average discharge capacity of ARLB with HVO/G as anode has a considerable improvement over that of HVO/CNTs and HVO, whatever the current rate used. Moreover, we find that the diffusion coefficient of lithium-ion increases by an order of magnitude through the theoretical calculation for HVO/G ARLB. The new ARLB with HVO/G electrode is a potential energy storage system with great advantages, such as simple preparation, easy assembly process, excellent safety and low-cost environmental protection.

Aqueous rechargeable lithium-ion batteries (ARLBs) are regarded as a competitive challenger for large-scale energy storage systems because of their high safety, modest cost, and green nature.     

Molecular insights into the ‘defects’ network in the thermosets and the influence on the mechanical performance

The introduction of ‘defects’ to the thermoset crosslinking network is one of the most applicable strategies for improving the modulus and toughness simultaneously. However, the reinforcement effect disappears when the ‘defects’ proportion exceeds the threshold. The speculated mechanism was that the aggregation and entanglement of the ‘defects’ chains changed the matrix topology, making the stacking structure more compact. However, the ‘defects’ are hardly directly observed in the experiment. As the result, the relationship between the ‘defects’ proportion and the package state of the matrix, and the effect on the material''s mechanical performance was not explored. Herein, the network of bisphenol-A diglycidyl (DGEBA) with diethyltoluenediamine (DETDA) as the hardener was constructed using MD simulation, and n-butylamine was decorated on the matrix by replacing a proportion of DETDA acting as the ‘defects’. The results indicated that the aliphatic chains aggregated and entangled at a low concentration, occupying the voids in the rigid aromatic crosslinking structure, thus lowering the free volume. The strong non-bonding interactions drew the matrix segments close together, thus reinforcing the resin. However, the microphases formed by the aliphatic chains no longer filled the voids but created a new free volume and loosened the network when the content increased, which reduced the mechanical performance of the material. The experimental results were consistent with the findings in the simulations. The moduli of the resin increased with the increase in the n-butylamine content first and then declined. The maximum moduli of the thermosets was 3.4 GPa in S₃₀, which was about 25% higher compared with the control; the corresponding elongation at break was 8.9%, which was about 46% improved compared with the control.

The introduction of ‘defects’ to the thermoset crosslinking network is one of the most applicable strategies for improving the modulus and toughness simultaneously.