气血瘀滞型原发性痛经中医身心同治的临床疗效观察与分析

目的：观察基于中医理论的身心同治方法对气血瘀滞型原发性痛经的临床疗效。方法：将120例气血瘀滞型原发性痛经的门诊患者随机分为治疗组和对照组各60例,2组均给予中药治疗,治疗组在中药治疗的基础上进行心理干预,3个月后观察2组有效率。结果：治疗组总有效率为93.33%,对照组总有效率为80.00%,治疗组总有效率高于对照组,且具有统计学意义（P<0.05）。结论：采用身心同治的综合干预方法治疗气血瘀滞型原发性痛经临床效果显著。     

中国不同地区三棱的化学特征比较

Objective: To compare the chemical characters of Sparganii Rhizoma from different areas via chromatographic analysis and to establish a sensitive LC/MS method for quality assessment of Sparganii Rhizoma.Methods: Under the optimised HPLC-PDA chromatographic conditions,twenty batches of Sparganii Rhizoma were analyzed by HPLC fingerprints.Principal component analysis(PCA),orthogonal projections to latent structures discriminant analysis(OPLS-DA)and hierarchical cluster analysis(HCA)were performed based on all peak areas of Sparganii Rhizoma fingerprints.Meanwhile,part of common peaks were subsequently quantified by UFLC-QTRAP-MS.Results: The similarity values of HPLC fingerprints fluctuated in a wide range of 0.511–0.973,which showed variable differences of chemical characters among Sparganii Rhizoma from twenty habitats.PCA,OPLS-DA and HCA indicated that samples could be divided into five groups with different chemical characters,which generally corresponded with their geographical distributions.A total of 31 peaks in HPLC fingerprints were marked,and eight of them were identified and quantified.The quantitative result was generally in agreement with the classifications based on HPLC fingerprints,which indicated that Sparganii Rhizoma samples from eastern China mostly contained more contents including phenolic acids and flavonoids.Conclusion: This study not only proved that there were relationships between geographic distributions and internal chemical compositions of plants,which could provide evidence to the traditional Chinese medicine concept "geo-authentic",but also supplied a sensitive and rapid simultaneous quantitive method for the quality estimation of Sparganii Rhizoma.     

《黄帝内经》经脉理论与络脉理论是各自独立的理论

经脉理论与络脉理论是各自不同的理论,不能合并起来研究,经络研究就是因为选择了经脉和络脉不分的经络概念为研究对象,并误解为经络系统研究,实际是以经络取代经脉,以穴位连结线取代经脉循行线进行检测,抛弃经脉理论研究而陷入找经络系统形态结构及相关物质的研究误区,所以无法取得科学的结论。     

基于网络药理学探讨“黄芪-当归-大枣”角药补气养血的分子机制＊

目的 运用网络药理学探讨“黄芪-当归-大枣”角药补气养血的分子机制。方法 利用中药系统药理学TCMSP数据库收集黄芪、当归和大枣的化学成分，并预测作用靶点。通过STRING数据库和Cytoscape软件构建蛋白互作PPI网络，获得关键靶点。借助DAVID数据库对筛选靶点进行GO功能和KEGG通路富集分析。进一步构建气血两虚小鼠模型，灌胃“黄芪-当归-大枣”水煎剂，检测各组PTGS₂ mRNA表达水平与肿瘤坏死因子-α(TNF-α)含量，完成验证实验。结果 筛选得到“黄芪-当归-大枣”角药化学成分47个，预测作用靶点43个，关键靶点10个，生物功能主要定位在内质网膜和线粒体，通过介导癌症信号通路，TNF、P53、Foxo、HIF-1信号通路，钙信号通路等发挥补气养血作用。动物实验显示“黄芪-当归-大枣”角药可调控气血两虚小鼠中PTGS₂ mRNA表达，降低TNF-α释放，进而抑制TNF信号通路的激活，进一步证实了预测的可靠性。结论 本研究深入诠释“黄芪-当归-大枣”角药补气养血作用多成分、多靶点和多通路的复杂机制，为其进一步临床应用和功能食品开发提供科学依据。     

头孢地尼与其他6种β-内酰胺类的体外抗菌活性研究

目的　比较头孢地尼与头孢克肟、头孢克洛、头孢呋辛、头孢拉定、头孢氨苄、阿莫西林对临床常见致病菌的体外抗菌活性。方法　采用琼脂平板稀释法测定最低抑菌浓度 (MIC)。结果　头孢地尼对金黄色葡萄球菌、表皮葡萄球菌的敏感率分别为 73 3%、90 % ,抗菌活性显著强于头孢克肟 ;对肺炎克雷伯氏菌的活性与头孢克肟相似 ,强于受试第一代、二代头孢菌素 ;对大肠埃希氏菌活性强于其他 6种抗生素 ;对变形菌活性与头孢克肟相似。阴沟肠杆菌、柠檬酸杆菌属对受试药物耐药性均较高 ,其中头孢地尼和头孢克肟的敏感率 40 %左右 ,较其他 5种抗生素为强。结论　头孢地尼对大部分革兰氏阳性及革兰氏阴性菌具有较强的抗菌活性。     

榆柏烧烫伤喷雾剂的药效学研究

目的 进行榆柏烧烫伤喷雾剂与其功能主治有关的药效学研究.方法 采用小鼠扭体法和甩尾法分别观察榆柏烧烫伤喷雾剂的镇痛作用;运用二甲苯致小鼠耳廓肿胀模型、血管通透性试验分别评价榆柏烧烫伤喷雾剂的抗炎作用.建立Ⅱ度烫伤模型,记录小鼠背部皮肤结痂时间和创面愈合时间,并进行病理组织学观察.结果 榆柏烧烫伤喷雾剂高、中、低3个剂量组与对照组比较,均具有良好的镇痛作用和抗炎作用,能明显延长小鼠的痛反应潜伏期,显著降低毛细血管通透性,减少炎症渗出,减轻水肿;具有显著提高烫伤小鼠愈合率、缩短愈合时间、提高创面愈合率、促进创面愈合的作用,对烫伤小鼠病理组织学观察的3个方面均有不同程度的改善.结论 榆柏烧烫伤喷雾剂对烧烫伤皮肤有促愈合作用,且具有抗炎、镇痛作用.     

Photocatalytic Hydrogen Evolution from Artificial Seawater Splitting over Amorphous Carbon Nitride: Optimization and Process Parameters Study via Response Surface Modeling

Photocatalytic water splitting has garnered tremendous attention for its capability to produce clean and renewable H₂ fuel from inexhaustible solar energy. Until now, most research has focused on scarce pure water as the source of H₂, which is not consistent with the concept of sustainable energy. Hence, the importance of photocatalytic splitting of abundant seawater in alleviating the issue of pure water shortages. However, seawater contains a wide variety of ionic components which have unknown effects on photocatalytic H₂ production. This work investigates photocatalytic seawater splitting conditions using environmentally friendly amorphous carbon nitride (ACN) as the photocatalyst. The individual effects of catalyst loading (X₁), sacrificial reagent concentration (X₂), salinity (X₃), and their interactive effects were studied via the Box–Behnken design in response surface modeling towards the H₂ evolution reaction (HER) from photocatalytic artificial seawater splitting. A second-order polynomial regression model is predicted from experimental data where the variance analysis of the regressions shows that the linear term (X₁, X₂), the two-way interaction term X₁X₂, and all the quadratic terms (X₁₂, X₂₂, X₂₃) pose significant effects towards the response of the HER rate. Numerical optimization suggests that the highest HER rate is 7.16 µmol/h, achievable by dosing 2.55 g/L of ACN in 45.06 g sea salt/L aqueous solution containing 17.46 vol% of triethanolamine. Based on the outcome of our findings, an apparent effect of salt ions on the adsorption behavior of the photocatalyst in seawater splitting with a sacrificial reagent has been postulated.     

提纯孕马血清促性腺激素的中试生产研究

孕马血清经偏磷酸、乙醇分级沉淀得比活为409IU／mg的粗品，回收率为47．3％。粗品经离子交换层析得到比活为1455IU／mg的精品，回收率达97.4％。再过柱层析得比活为2400IU／mg的产品。     

人参皂苷Rg_1通过作用于炎性因子和糖皮质激素受体减少金葡菌对肺上皮细胞的损伤(英文)

本文旨在探讨人参皂苷Rg_1体外对金葡菌感染肺上皮细胞所致病变的作用及其可能的机制。实验采用体外大鼠原代肺上皮细胞感染模型。RT-PCR和Western blot方法观察肺细胞中integrinβ1,NF-κB和糖皮质激素受体(GR)等相关因子的表达。结果表明人参皂苷Rg_1可以明显抑制金葡菌感染后肺上皮细胞整合素integrinβ1的表达,下调炎性因子NF-κB,ICAM-1,TNF-α,IL-2和IL-6,上调GR的表达。人参皂苷Rg_1可以明显抑制金葡菌感染肺上皮细胞后的炎性因子,从而对肺脏起到一定的保护作用。     

四种无创心电检查方法诊断冠心病的评价

目的　探讨普通心电图(ECG)、平板运动试验(TMT)、电话传输远程心电监测(TTM)、动态心电图(Holtermonitor)对冠心病(CHD)的诊断价值。方法　选择6 0例疑诊CHD的患者,将其ECG、TMT、TTM、Holter和冠状动脉造影(CA)检查结果进行对比分析,以CA为金标准计算以上四种无创心电检查方法的灵敏度、特异度等指标。结果　ECG、TMT、TTM、Holter的灵敏度分别为5 5 . 0 0 %、71 .4 3%、72 . 5 0 %、70 .0 0 % ;特异度分别为6 0 .0 0 %、76 .92 %、75 . 0 0 %、75 . 0 0 % ;准确度分别为5 6 .6 7%、74 . 0 7%、73. 33%、71. 88%。将TTM和TMT联合试验可使灵敏度提高到85 . 71% ,特异度提高到84 . 6 2 %。结论　ECG、TMT、TTM、Holter是诊断CHD的常用且重要的无创检查方法。