The effect of ketoprofen creams on periodontal disease in rhesus monkeys

Ketoprofen creams were evaluated for the treatment of periodontal disease in a placebo-controlled, double-blind study in the rhesus monkeys, Macaca mulatta. Two formulations containing ketoprofen (1%), with or without vitamin E, were evaluated against appropriate controls (8 monkeys per group). Two weeks prior to treatment, the animals received prophylaxis on only the left side of the mouth (spontaneous model). Selected teeth on the right side of the mouth were ligated (ligature model). The creams were administered to the gingiva once daily at a standard dose of 1.8 ml per monkey for 6 months. Clinical assessments were made 2 wk before initiation, at baseline and 1, 2, 3 and 6 months post-treatment. The clinical parameters included plaque formation, gingival redness, edema, bleeding on probing and Ramfjord Attachment Level measurements (RAL). Radiographs were taken at 2 wk before initiation, baseline and at 3 and 6 months post-treatment. Digital subtraction radiography was used to measure vertical linear bone loss along the interproximal root surfaces of the left and right mandibular first molars. Gingival crevicular fluid (GCF) was collected for biochemical assays on PGE₂, TxB₂, LTB₄, IL-1β and TNFα. There were no significant differences among groups with respect to gingival indices. Radiographic data demonstrated significant positive effects on bone activity in both groups treated with ketoprofen formulations with improvement over time in the ligature model (0.01 ≤p≤ 0.04). The placebo group exhibited bone loss of 1.96±0.48 and 1.40±0.56 mm per site at 3 and 6 months, respectively. The group treated with ketoprofen cream showed an apparent bone gain of 0.28±0.41 and 0.78±0.47 mm per site at 3 and 6 months, respectively. The group treated with ketoprofen cream containing vitamin E showed a mean bone loss of 0.41–0.48 mm per site at 3 months with improvement to an apparent bone gain of 0.31±0.44 mm per site at 6 months. The biochemical data demonstrated early and significant suppression of GCF-LTB₄ by both ketoprofen formulations at 1 month, which preceded the significant suppression of GCF-PGE₂ at 2 and 3 months in the ligature model (p≤0.003) and at 2 to 6 months in the spontaneous model (p≤0.02). We conclude that ketoprofen at 1% level in suitable topical vehicles can effectively inhibit GCF-LTB₄ and GCF-PGE₂ and positively alter alveolar bone activity in the ligature-induced model of periodontitis in the monkey.     

Competitive adsorption versus surface complexation as models for the simultaneous adsorption of metal complexes and free ligands

A statistical thermodynamical approach to the study of anion-induced adsorption of Cd(II) from halide solutions is presented. The simultaneous adsorption of metal complex and ligand is introduced in the isotherms by considering two possible mechanisms — competitive adsorption and surface complexation. These isotherms have been tested for the system Cd(II) in KBr at several ionic strengths. The experimental surface excesses of Cd(II) calculated from single-step chronocoulometry can be simulated, giving an explanation for the desorption of the metal complex at positive potentials. Also, the change in ligand adsorption promoted by the adsorption of the metal complex has been calculated. Both approaches lead to the conclusion that the anionic tricoordinate metal complex CdBr₃^? and the tetracoordinate CdBr₄^2? are the absorbed species on the electrode surface, with CdBr₄^2? dominating at higher bromide concentrations.     

Measurement of proteases in human subgingival dental plaque by fluorescence polarization

Fluorescence polarization (FP) was examined as a rapid quantitative method to assay the proteases in subgingival plaque. Protease activity was measured by a decrease in FP at 0.5-min intervals over 5 min, using BODIPY®--casein, a protein substrate. To quantitate activity, the least absolute deviation (LAD) slope for each assay was determined. Protease activity increased with the quantity of plaque (r=0.416, P<0.001). Of the 208 subgingival plaque samples, 87 contained detectable protease activity, with a mean of about 4 μg trypsin equivalents above a general background of 1 μg per site. The mean plaque protease activity of 89 paired samples from 15 individuals had decreased by 1.1 μg trypsin equivalents per site when measured at 8 months after tooth scaling and root planing (P<0.01). Most isolates of Porphyromonas gingivalis, Treponema denticola, Prevotella nigrescens, and Prevotella intermedia implicated in the pathogenesis of adult periodontitis exhibited high activity in the FP assay. The assay is rapid, quantitative and requires only one-tenth of the plaque sampled using a single pass with a Gracey curette at a single tooth site.     

The influence of bone mechanical properties and implant fixation upon bone loading around oral implants.

Finite element models were created to study the stress and strain distribution around a solitary BAnemark implant. The influence of a number of clinically relevant parameters was examined: bone-implant interface (fixed bond versus frictionless free contact), bone elastic properties, unicortical versus bicortical implant fixation and the presence of a lamina dura. Bone loading patterns in the vicinity of the implant seem to be very sensitive to these parameters. Hence they should be integrated correctly in numerical models of in vivo behaviour of oral implants. This necessitates the creation of patient-dependent finite element models.     

In vitro resistance to fracture of porcelain inlays bonded to tooth

To assess the resistance to fracture of ceramic inlays, extracted sound human molars were restored with different ceramic materials and glass-ionomer or composite resin luting cement. Compression forces were applied to the specimens until they fractured, and maximal force developed, extent and location of fractures, and the micromorphology of the fractured surfaces were studied. Inlays made of Vitadur N with aluminum oxide profile and bonded with composite resin demonstrated the highest resistance among the restorative combinations, but their resistance was still lower than that of intact (control) teeth. The extent of fracture increased proportionally to the fracture resistance of the specimens. Scanning electron microscopic observation confirmed the effectiveness of the adhesion between composite resin and etched ceramic and the absence of sufficient micromechanical anchoring between glass-ionomer cement and etched ceramic.     

Studies on the castability of Co-Cr alloy for cast plates. Part 2: Effect of form of sprue attachment to the wax pattern

Co-Cr alloy is used more frequently than Ni-Cr alloy as a non-precious alloy for cast plates in Japan. However, since the melting point of Co-Cr alloy is very high, about 1300 degrees C, and since it oxidizes easily, a vacuum-pressure casting machine capable of melting this alloy in a reducing atmosphere has recently been developed. Using this vacuum-pressure casting machine, the authors studied the effects on the castability of Co-Cr alloy due to the form of sprue attachment to the wax pattern. The results clarified that in the vacuum-pressure casting method, the form of sprue attachment to the wax pattern has a significant effect (p less than 0.01) on the castability of Co-Cr alloy.     

Giant cell reaction to ameloblastoma: an immunohistochemical and ultrastructural study of a case

Histopathologic, immunohistochemical, and ultrastructural features of the giant cells appearing in the tissue surrounding an ameloblastoma are presented. These giant cells exhibited strong activity of a highly stable form of acid phosphatase, and were positive for alpha-1-antichymotrypsin. Ultrastructurally, the cytoplasm of the giant cells contained numerous mitochondria, and the cell membrane was comparatively smooth. Therefore, it is proposed that these multinucleated giant cells are reactive to the invasion of ameloblastoma and are histiocytic in origin.     

Repair of periodontal angular bony defects evaluated by one- and two-dimensional radiographic analysis

The healing of eighteen angular periodontal bony defects was evaluated radiographically 1 year after treatment. The pretreatment and posttreatment radiographs were exposed and processed in such a way that identical images were obtained. There was no correlation between one- and two-dimensional expressions of healing in such defects, and relative area changes were consistently larger than relative linear changes of the same defects. The area gain of bone was strongly correlated to the following morphologic variables: length of bone surface, original defect area, and height of the defect.